pycom03g10230

pollen Ole e 1 allergen and extensin family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015315
Physical Location & Seq
Forward (+)
9335863 .. 9336971
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGCGCTTTCCTGGATTCTCCCAGCACTTCTCTTTGCTTTGGTTCTTGCAAATGTTGAACTCTCCACATGCCAAGTTCTGAAGGCCAAGGTCACTTGCCTTGACTGCCATCAAAATTATGATTTCTCAGGAATCAAGGTTTTGGTGAAGTGTGATAAAGTGAAAAAGATGGCAATGGCAATAACAGAAAATGACGGCTCTTTTGAAGCAAAGCTCCCCTCAAACAACAACACTAAATCTCCACATGCTAAGAACTGCTTTGCCAGGATTCTTGGAGGACCAGATCAGCTCTATGCCTCCAGGAAGTTCATGGCATCTGAGATTGTCAAGACCCAAGACTCCAACTCTTATACCACAACAACTCCTCTTGGCTTCTCCACATCATGCCCTTTGAACATCAAAGATGCAGCCTGTAAAGCCATGAACAAGTTTGGCTCGTCCAAGACTATCAATCTACCTCTGCCACCAGAGTGGGGTTTGGCACCTTCAAGCTATTATATTCCTTTCATCCCCATCATTGGGATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

177

Amino Acids

19.17

Weight (kDa)

8.95

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 95 1.6e-06 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G22430
fragaria_vesca FvH4_3g25370 FvH4_3g25370
malus_domestica MD03G1148100.v1.1 MD11G1167600.v1.1
prunus_persica Prupe.6G134300_v2.0.a1
pyrus_communis pycom03g10230
rosa_laevigata RLG00000034436
rosa_multiflora Rmu_sc0017747.1_g000005
rosa_roxburghii Rroxscaffold_1G00034660
rosa_rugosa Rorug05G0231100
rosa_samantha Rh5AG310900 Rh5BG319300 Rh5CG345200 Rh5DG329900
rosa_wichuraiana Rw5G029010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 481
AcuI CTGAAG 1 cut(s) 101
AfiI CCNNNNNNNGG 3 cut(s) 473, 518, 519
AgsI TTSAA 4 cut(s) 59, 206, 394, 489
AjnI CCWGG 3 cut(s) 11, 263, 299
AleI CACNNNNGTG 1 cut(s) 469
AluBI AGCT 3 cut(s) 214, 289, 492
AluI AGCT 3 cut(s) 214, 289, 492
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 185, 217
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 157
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 481
BbvI GCAGC 1 cut(s) 419
BccI CCATC 3 cut(s) 117, 163, 521
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 3 cut(s) 13, 265, 301
BciVI GTATCC 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 516
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 3 cut(s) 13, 265, 301
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 1 cut(s) 483
BmrFI CCNGG 3 cut(s) 13, 265, 301
BmsI GCATC 2 cut(s) 323, 394
BpmI CTGGAG 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 87
BsaXI ACNNNNNCTCC 4 cut(s) 223, 253, 346, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 473, 518, 519
Bse3DI GCAATG 1 cut(s) 180
BseBI CCWGG 3 cut(s) 13, 265, 301
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 507
BseLI CCNNNNNNNGG 3 cut(s) 473, 518, 519
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 141, 309
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 419
BseYI CCCAGC 1 cut(s) 22
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 481
BslI CCNNNNNNNGG 3 cut(s) 473, 518, 519
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 283
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 140, 310
BspLI GGNNCC 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 283
BssT1I CCWWGG 1 cut(s) 87
Bst2UI CCWGG 3 cut(s) 13, 265, 301
BstDEI CTNAG 3 cut(s) 127, 249, 318
BstF5I GGATG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 2 cut(s) 105, 416
BstNI CCWGG 3 cut(s) 13, 265, 301
BstNSI RCATGY 2 cut(s) 72, 248
BstSCI CCNGG 3 cut(s) 11, 263, 299
BstV1I GCAGC 1 cut(s) 419
BstXI CCANNNNNNTGG 1 cut(s) 471
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 1 cut(s) 507
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 278
CviAII CATG 5 cut(s) 69, 245, 310, 384, 421
CviJI RGCY 9 cut(s) 86, 198, 214, 289, 372, 410, 419, 435, 492
CviKI_1 RGCY 9 cut(s) 86, 198, 214, 289, 372, 410, 419, 435, 492
DdeI CTNAG 3 cut(s) 127, 249, 318
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 101
EcoRII CCWGG 3 cut(s) 11, 263, 299
EcoT14I CCWWGG 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 5 cut(s) 72, 248, 313, 387, 424
FaiI YATR 9 cut(s) 70, 120, 246, 294, 311, 351, 385, 422, 498
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 5 cut(s) 68, 244, 309, 383, 420
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 1 cut(s) 494
Fsp4HI GCNGC 1 cut(s) 408
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 86
HhaI GCGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 72, 248, 313, 387, 424
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 16, 132, 268, 338
HphI GGTGA 1 cut(s) 157
Hpy188I TCNGA 2 cut(s) 81, 319
Hpy188III TCNNGA 2 cut(s) 129, 328
HpyAV CCTTC 2 cut(s) 76, 495
HpyCH4V TGCA 2 cut(s) 50, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 416
HpyF3I CTNAG 3 cut(s) 127, 249, 318
Hsp92II CATG 5 cut(s) 72, 248, 313, 387, 424
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 219
Lsp1109I GCAGC 1 cut(s) 419
LweI GCATC 2 cut(s) 323, 394
MaeIII GTNAC 1 cut(s) 91
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MluCI AATT 1 cut(s) 115
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 5 cut(s) 229, 269, 307, 375, 468
MseI TTAA 1 cut(s) 529
MslI CAYNNNNRTG 1 cut(s) 469
MspR9I CCNGG 3 cut(s) 13, 265, 301
MvaI CCWGG 3 cut(s) 13, 265, 301
MwoI GCNNNNNNNGC 2 cut(s) 105, 416
NdeII GATC 1 cut(s) 283
NlaIII CATG 5 cut(s) 72, 248, 313, 387, 424
NlaIV GGNNCC 1 cut(s) 483
NmuCI GTSAC 1 cut(s) 91
NspI RCATGY 2 cut(s) 72, 248
OliI CACNNNNGTG 1 cut(s) 469
PfeI GAWTC 3 cut(s) 16, 132, 268
PfoI TCCNGGA 2 cut(s) 11, 299
PkrI GCNGC 1 cut(s) 409
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Psp6I CCWGG 3 cut(s) 11, 263, 299
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 3 cut(s) 11, 263, 299
PspN4I GGNNCC 1 cut(s) 483
PspPI GGNCC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 469
SaqAI TTAA 1 cut(s) 529
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 1 cut(s) 278
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 13, 265, 301
SetI ASST 8 cut(s) 93, 141, 216, 291, 460, 487, 494, 529
SfaNI GCATC 2 cut(s) 323, 394
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 469
Sse9I AATT 1 cut(s) 115
StyD4I CCNGG 3 cut(s) 11, 263, 299
StyI CCWWGG 1 cut(s) 87
TasI AATT 1 cut(s) 115
TfiI GAWTC 3 cut(s) 16, 132, 268
Tru1I TTAA 1 cut(s) 529
Tru9I TTAA 1 cut(s) 529
TseFI GTSAC 1 cut(s) 91
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 298, 437, 496
VpaK11BI GGWCC 1 cut(s) 278
XceI RCATGY 2 cut(s) 72, 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.