pycom03g14340

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0020342
Physical Location & Seq
Forward (+)
15667443 .. 15667850
408 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGCCCTTTCGTTGTCAAAAGATAGCTTTGAGTTGCTGTTGTACACGGTGTGGGGCATCTGGACTGACCGAAACTCTTTATTATGGTGGGGTGTAGTCTCGAGTCTTGGAGATATTCGTTTCAGAGTGCAGTCGTGGCAGACAGAATATAAAAAGTGGCATGGTAGTGAACTAGGCTACAAGATGAAAGAGCCTCAGAGGTGGTGTAAACCAGATCAAGCGTGGCTGAAAATTAATTTTGACGGGGCATGGGATGAGCATCGTCAAATGGGTGGCTGCGGAGTGGTGGTAAGGGACACAAGGGGGAAGTTTGCGGAGAAGCTTGGGGATGCCCAGCGGCAACTAGAAGGAGATGCCTTAATGGTGGTGGCAAGTATTCAAAAGGAGGCTAGCGCCAATTTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

136

Amino Acids

15.6

Weight (kDa)

7.76

Isoelectric Point (pI)

24.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020342)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g47542
pyrus_communis pycom03g14340
rosa_laevigata RLG00000021637
rosa_roxburghii Rroxscaffold_2G00084690
rosa_samantha Rh2AG590700 Rh2DG613500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 279, 314, 337
AdeI CACNNNGTG 1 cut(s) 51
AfaI GTAC 1 cut(s) 44
AgsI TTSAA 1 cut(s) 380
AluBI AGCT 2 cut(s) 27, 322
AluI AGCT 2 cut(s) 27, 322
Alw26I GTCTC 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 100
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 276
AseI ATTAAT 1 cut(s) 234
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 4
AsuNHI GCTAGC 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 100
BbvI GCAGC 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 3 cut(s) 173, 344, 390
BfoI RGCGCY 1 cut(s) 396
BisI GCNGC 2 cut(s) 277, 338
BlsI GCNGC 2 cut(s) 278, 339
BmeT110I CYCGRG 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 4
BmsI GCATC 4 cut(s) 66, 268, 319, 343
BmtI GCTAGC 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bse8I GATNNNNATC 1 cut(s) 258
BseGI GGATG 2 cut(s) 259, 334
BseJI GATNNNNATC 1 cut(s) 258
BseMII CTCAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 263
BseYI CCCAGC 1 cut(s) 333
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 1 cut(s) 5
BsiHKCI CYCGRG 1 cut(s) 100
BslFI GGGAC 1 cut(s) 308
BsmAI GTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 100
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 1 cut(s) 214
BspACI CCGC 3 cut(s) 279, 314, 337
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 393
BsrGI TGTACA 1 cut(s) 42
BssMI GATC 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 49
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 1 cut(s) 195
BstF5I GGATG 2 cut(s) 259, 334
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 1 cut(s) 217
BstMAI GTCTC 1 cut(s) 103
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 263
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 259, 334
Cac8I GCNNGC 1 cut(s) 391
CfoI GCGC 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 43
CviAII CATG 2 cut(s) 161, 249
CviJI RGCY 8 cut(s) 5, 27, 177, 193, 226, 276, 322, 389
CviKI_1 RGCY 8 cut(s) 5, 27, 177, 193, 226, 276, 322, 389
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
DraIII CACNNNGTG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 100
FaeI CATG 2 cut(s) 164, 252
FaiI YATR 4 cut(s) 85, 150, 162, 250
FaqI GGGAC 1 cut(s) 308
FatI CATG 2 cut(s) 160, 248
Fnu4HI GCNGC 2 cut(s) 277, 338
FokI GGATG 2 cut(s) 266, 341
Fsp4HI GCNGC 2 cut(s) 277, 338
FspBI CTAG 3 cut(s) 173, 344, 390
GlaI GCGC 1 cut(s) 394
GluI GCNGC 2 cut(s) 277, 338
GsaI CCCAGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 395
Hin1II CATG 2 cut(s) 164, 252
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 1 cut(s) 103
Hpy166II GTNNAC 3 cut(s) 45, 170, 209
Hpy188I TCNGA 2 cut(s) 125, 198
Hpy188III TCNNGA 2 cut(s) 61, 100
Hpy8I GTNNAC 3 cut(s) 45, 170, 209
HpyAV CCTTC 1 cut(s) 341
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 2 cut(s) 164, 252
HspAI GCGC 1 cut(s) 393
Kzo9I GATC 1 cut(s) 214
LpnPI CCDG 3 cut(s) 46, 225, 347
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 4 cut(s) 66, 268, 319, 343
MaeI CTAG 3 cut(s) 173, 344, 390
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MluCI AATT 3 cut(s) 231, 235, 397
MlyI GAGTC 1 cut(s) 112
MnlI CCTC 3 cut(s) 192, 204, 379
MseI TTAA 3 cut(s) 234, 359, 406
MslI CAYNNNNRTG 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 136
NdeII GATC 1 cut(s) 214
NheI GCTAGC 1 cut(s) 389
NlaIII CATG 2 cut(s) 164, 252
PaeR7I CTCGAG 1 cut(s) 100
PkrI GCNGC 2 cut(s) 278, 339
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PshBI ATTAAT 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 165
SaqAI TTAA 3 cut(s) 234, 359, 406
SatI GCNGC 2 cut(s) 277, 338
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 112
SetI ASST 3 cut(s) 29, 203, 324
SfaNI GCATC 4 cut(s) 66, 268, 319, 343
Sfr274I CTCGAG 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 100
SmoI CTYRAG 1 cut(s) 100
Sse9I AATT 3 cut(s) 231, 235, 397
SsiI CCGC 3 cut(s) 279, 314, 337
SspMI CTAG 3 cut(s) 173, 344, 390
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 1 cut(s) 101
TaqII GACCGA 1 cut(s) 84
TasI AATT 3 cut(s) 231, 235, 397
TatI WGTACW 1 cut(s) 42
TauI GCSGC 1 cut(s) 340
Tru1I TTAA 3 cut(s) 234, 359, 406
Tru9I TTAA 3 cut(s) 234, 359, 406
TseI GCWGC 1 cut(s) 276
TspDTI ATGAA 1 cut(s) 200
VspI ATTAAT 1 cut(s) 234
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 100
XspI CTAG 3 cut(s) 173, 344, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.