Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g21853 FvH4_3g21921 FvH4_3g21922 |
| prunus_persica | Prupe.4G025900_v2.0.a1 |
| pyrus_communis | pycom03g15910 |
| rosa_roxburghii | Rroxscaffold_6G00411980 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AbsI | CCTCGAGG | 1 cut(s) | 152 |
| Acc36I | ACCTGC | 1 cut(s) | 464 |
| AccI | GTMKAC | 1 cut(s) | 37 |
| AccII | CGCG | 2 cut(s) | 29, 558 |
| AccIII | TCCGGA | 1 cut(s) | 448 |
| AciI | CCGC | 4 cut(s) | 268, 550, 556, 558 |
| AclWI | GGATC | 1 cut(s) | 190 |
| AcoI | YGGCCR | 1 cut(s) | 265 |
| AfaI | GTAC | 1 cut(s) | 546 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 205, 313, 363, 433 |
| AflIII | ACRYGT | 1 cut(s) | 27 |
| AgsI | TTSAA | 1 cut(s) | 161 |
| AjnI | CCWGG | 2 cut(s) | 312, 426 |
| AluBI | AGCT | 4 cut(s) | 165, 256, 393, 484 |
| AluI | AGCT | 4 cut(s) | 165, 256, 393, 484 |
| Alw21I | GWGCWC | 1 cut(s) | 395 |
| Alw26I | GTCTC | 2 cut(s) | 87, 329 |
| AlwI | GGATC | 1 cut(s) | 190 |
| Ama87I | CYCGRG | 3 cut(s) | 152, 257, 485 |
| Aor13HI | TCCGGA | 1 cut(s) | 448 |
| AoxI | GGCC | 1 cut(s) | 265 |
| ApeKI | GCWGC | 2 cut(s) | 253, 481 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 361, 393 |
| AseI | ATTAAT | 1 cut(s) | 18 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 213 |
| AspS9I | GGNCC | 2 cut(s) | 451, 498 |
| AsuC2I | CCSGG | 1 cut(s) | 364 |
| AsuHPI | GGTGA | 1 cut(s) | 139 |
| AvaI | CYCGRG | 3 cut(s) | 152, 257, 485 |
| AvaII | GGWCC | 2 cut(s) | 451, 498 |
| BanII | GRGCYC | 1 cut(s) | 395 |
| BbsI | GAAGAC | 1 cut(s) | 374 |
| Bbv12I | GWGCWC | 1 cut(s) | 395 |
| BbvI | GCAGC | 2 cut(s) | 265, 493 |
| BccI | CCATC | 2 cut(s) | 152, 485 |
| BceAI | ACGGC | 1 cut(s) | 280 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 238, 272 |
| BciT130I | CCWGG | 2 cut(s) | 314, 428 |
| BcnI | CCSGG | 1 cut(s) | 364 |
| BcoDI | GTCTC | 2 cut(s) | 87, 329 |
| BfuAI | ACCTGC | 1 cut(s) | 464 |
| BglII | AGATCT | 1 cut(s) | 114 |
| BisI | GCNGC | 3 cut(s) | 254, 268, 482 |
| BlsI | GCNGC | 3 cut(s) | 255, 269, 483 |
| Bme1390I | CCNGG | 3 cut(s) | 314, 364, 428 |
| Bme18I | GGWCC | 2 cut(s) | 451, 498 |
| BmeT110I | CYCGRG | 3 cut(s) | 152, 257, 485 |
| BmgT120I | GGNCC | 2 cut(s) | 451, 498 |
| BmiI | GGNNCC | 1 cut(s) | 500 |
| BmrFI | CCNGG | 3 cut(s) | 314, 364, 428 |
| BmsI | GCATC | 1 cut(s) | 99 |
| BpiI | GAAGAC | 1 cut(s) | 374 |
| BpuEI | CTTGAG | 1 cut(s) | 89 |
| BpuMI | CCSGG | 1 cut(s) | 364 |
| BsaBI | GATNNNNATC | 1 cut(s) | 113 |
| BsaJI | CCNNGG | 3 cut(s) | 5, 328, 556 |
| BsaWI | WCCGGW | 2 cut(s) | 134, 448 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 537, 567 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 205, 313, 363, 433 |
| Bse118I | RCCGGY | 1 cut(s) | 245 |
| Bse8I | GATNNNNATC | 1 cut(s) | 113 |
| BseAI | TCCGGA | 1 cut(s) | 448 |
| BseBI | CCWGG | 2 cut(s) | 314, 428 |
| BseDI | CCNNGG | 3 cut(s) | 5, 328, 556 |
| BseGI | GGATG | 3 cut(s) | 288, 496, 516 |
| BseJI | GATNNNNATC | 1 cut(s) | 113 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 205, 313, 363, 433 |
| BseX3I | CGGCCG | 1 cut(s) | 265 |
| BseXI | GCAGC | 2 cut(s) | 265, 493 |
| Bsh1236I | CGCG | 2 cut(s) | 29, 558 |
| Bsh1285I | CGRYCG | 1 cut(s) | 268 |
| BshFI | GGCC | 1 cut(s) | 267 |
| BsiEI | CGRYCG | 1 cut(s) | 268 |
| BsiHKAI | GWGCWC | 1 cut(s) | 395 |
| BsiHKCI | CYCGRG | 3 cut(s) | 152, 257, 485 |
| BsiSI | CCGG | 4 cut(s) | 135, 246, 363, 449 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 205, 313, 363, 433 |
| BsmAI | GTCTC | 2 cut(s) | 87, 329 |
| BsmBI | CGTCTC | 2 cut(s) | 87, 329 |
| BsmI | GAATGC | 1 cut(s) | 441 |
| BsnI | GGCC | 1 cut(s) | 267 |
| BsoBI | CYCGRG | 3 cut(s) | 152, 257, 485 |
| Bsp1286I | GDGCHC | 1 cut(s) | 395 |
| Bsp13I | TCCGGA | 1 cut(s) | 448 |
| Bsp143I | GATC | 3 cut(s) | 114, 195, 465 |
| BspACI | CCGC | 4 cut(s) | 268, 550, 556, 558 |
| BspANI | GGCC | 1 cut(s) | 267 |
| BspEI | TCCGGA | 1 cut(s) | 448 |
| BspFNI | CGCG | 2 cut(s) | 29, 558 |
| BspLI | GGNNCC | 1 cut(s) | 500 |
| BspMI | ACCTGC | 1 cut(s) | 464 |
| BspPI | GGATC | 1 cut(s) | 190 |
| BsrFI | RCCGGY | 1 cut(s) | 245 |
| BssAI | RCCGGY | 1 cut(s) | 245 |
| BssECI | CCNNGG | 3 cut(s) | 5, 328, 556 |
| BssMI | GATC | 3 cut(s) | 114, 195, 465 |
| BssT1I | CCWWGG | 1 cut(s) | 5 |
| Bst2UI | CCWGG | 2 cut(s) | 314, 428 |
| BstC8I | GCNNGC | 3 cut(s) | 89, 247, 251 |
| BstDSI | CCRYGG | 2 cut(s) | 328, 556 |
| BstF5I | GGATG | 3 cut(s) | 288, 496, 516 |
| BstFNI | CGCG | 2 cut(s) | 29, 558 |
| BstKTI | GATC | 3 cut(s) | 117, 198, 468 |
| BstMAI | GTCTC | 2 cut(s) | 87, 329 |
| BstMBI | GATC | 3 cut(s) | 114, 195, 465 |
| BstMCI | CGRYCG | 1 cut(s) | 268 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 53 |
| BstNI | CCWGG | 2 cut(s) | 314, 428 |
| BstSCI | CCNGG | 3 cut(s) | 312, 362, 426 |
| BstUI | CGCG | 2 cut(s) | 29, 558 |
| BstV1I | GCAGC | 2 cut(s) | 265, 493 |
| BstV2I | GAAGAC | 1 cut(s) | 374 |
| BstX2I | RGATCY | 2 cut(s) | 114, 195 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 379 |
| BstYI | RGATCY | 2 cut(s) | 114, 195 |
| BstZI | CGGCCG | 1 cut(s) | 265 |
| BsuRI | GGCC | 1 cut(s) | 267 |
| BtgI | CCRYGG | 2 cut(s) | 328, 556 |
| BtsCI | GGATG | 3 cut(s) | 288, 496, 516 |
| BveI | ACCTGC | 1 cut(s) | 464 |
| Cac8I | GCNNGC | 3 cut(s) | 89, 247, 251 |
| Cfr10I | RCCGGY | 1 cut(s) | 245 |
| Cfr13I | GGNCC | 2 cut(s) | 451, 498 |
| Cfr42I | CCGCGG | 1 cut(s) | 559 |
| CsiI | ACCWGGT | 1 cut(s) | 312 |
| Csp6I | GTAC | 1 cut(s) | 545 |
| CviQI | GTAC | 1 cut(s) | 545 |
| DpnI | GATC | 3 cut(s) | 116, 197, 467 |
| DpnII | GATC | 3 cut(s) | 114, 195, 465 |
| EaeI | YGGCCR | 1 cut(s) | 265 |
| EagI | CGGCCG | 1 cut(s) | 265 |
| EciI | GGCGGA | 1 cut(s) | 539 |
| Ecl136II | GAGCTC | 1 cut(s) | 393 |
| EclXI | CGGCCG | 1 cut(s) | 265 |
| Eco130I | CCWWGG | 1 cut(s) | 5 |
| Eco24I | GRGCYC | 1 cut(s) | 395 |
| Eco47I | GGWCC | 2 cut(s) | 451, 498 |
| Eco52I | CGGCCG | 1 cut(s) | 265 |
| Eco53kI | GAGCTC | 1 cut(s) | 393 |
| Eco88I | CYCGRG | 3 cut(s) | 152, 257, 485 |
| EcoICRI | GAGCTC | 1 cut(s) | 393 |
| EcoO109I | RGGNCCY | 1 cut(s) | 498 |
| EcoRII | CCWGG | 2 cut(s) | 312, 426 |
| EcoT14I | CCWWGG | 1 cut(s) | 5 |
| EcoT22I | ATGCAT | 1 cut(s) | 58 |
| EcoT38I | GRGCYC | 1 cut(s) | 395 |
| ErhI | CCWWGG | 1 cut(s) | 5 |
| Esp3I | CGTCTC | 2 cut(s) | 87, 329 |
| FaiI | YATR | 3 cut(s) | 69, 71, 338 |
| FblI | GTMKAC | 1 cut(s) | 37 |
| Fnu4HI | GCNGC | 3 cut(s) | 254, 268, 482 |
| FokI | GGATG | 3 cut(s) | 275, 503, 503 |
| FriOI | GRGCYC | 1 cut(s) | 395 |
| Fsp4HI | GCNGC | 3 cut(s) | 254, 268, 482 |
| GluI | GCNGC | 3 cut(s) | 254, 268, 482 |
| HaeIII | GGCC | 1 cut(s) | 267 |
| HapII | CCGG | 4 cut(s) | 135, 246, 363, 449 |
| HinfI | GANTC | 2 cut(s) | 299, 527 |
| HpaII | CCGG | 4 cut(s) | 135, 246, 363, 449 |
| HphI | GGTGA | 1 cut(s) | 139 |
| Hpy166II | GTNNAC | 3 cut(s) | 38, 303, 531 |
| Hpy188I | TCNGA | 2 cut(s) | 169, 397 |
| Hpy188III | TCNNGA | 5 cut(s) | 106, 292, 352, 449, 520 |
| Hpy8I | GTNNAC | 3 cut(s) | 38, 303, 531 |
| HpyAV | CCTTC | 5 cut(s) | 203, 289, 489, 517, 545 |
| HpyCH4V | TGCA | 4 cut(s) | 56, 112, 340, 473 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 53 |
| Kpn2I | TCCGGA | 1 cut(s) | 448 |
| KroI | GCCGGC | 1 cut(s) | 245 |
| KroNI | GCCGGC | 1 cut(s) | 247 |
| KspI | CCGCGG | 1 cut(s) | 559 |
| Kzo9I | GATC | 3 cut(s) | 114, 195, 465 |
| LmnI | GCTCC | 2 cut(s) | 170, 398 |
| Lsp1109I | GCAGC | 2 cut(s) | 265, 493 |
| LweI | GCATC | 1 cut(s) | 99 |
| MabI | ACCWGGT | 1 cut(s) | 312 |
| MalI | GATC | 3 cut(s) | 116, 197, 467 |
| MboI | GATC | 3 cut(s) | 114, 195, 465 |
| MboII | GAAGA | 2 cut(s) | 337, 379 |
| MflI | RGATCY | 2 cut(s) | 114, 195 |
| MhlI | GDGCHC | 1 cut(s) | 395 |
| MluCI | AATT | 1 cut(s) | 15 |
| MluI | ACGCGT | 1 cut(s) | 27 |
| MlyI | GAGTC | 2 cut(s) | 308, 536 |
| MmeI | TCCRAC | 2 cut(s) | 192, 420 |
| Mph1103I | ATGCAT | 1 cut(s) | 58 |
| MroI | TCCGGA | 1 cut(s) | 448 |
| MroNI | GCCGGC | 1 cut(s) | 245 |
| MroXI | GAANNNNTTC | 1 cut(s) | 213 |
| MseI | TTAA | 2 cut(s) | 18, 59 |
| MspA1I | CMGCKG | 1 cut(s) | 558 |
| MspI | CCGG | 4 cut(s) | 135, 246, 363, 449 |
| MspR9I | CCNGG | 3 cut(s) | 314, 364, 428 |
| Mva1269I | GAATGC | 1 cut(s) | 441 |
| MvaI | CCWGG | 2 cut(s) | 314, 428 |
| MvnI | CGCG | 2 cut(s) | 29, 558 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 53 |
| NaeI | GCCGGC | 1 cut(s) | 247 |
| NciI | CCSGG | 1 cut(s) | 364 |
| NdeII | GATC | 3 cut(s) | 114, 195, 465 |
| NgoMIV | GCCGGC | 1 cut(s) | 245 |
| NlaIV | GGNNCC | 1 cut(s) | 500 |
| NsiI | ATGCAT | 1 cut(s) | 58 |
| PaeR7I | CTCGAG | 3 cut(s) | 152, 257, 485 |
| PctI | GAATGC | 1 cut(s) | 441 |
| PdiI | GCCGGC | 1 cut(s) | 247 |
| PdmI | GAANNNNTTC | 1 cut(s) | 213 |
| PflFI | GACNNNGTC | 2 cut(s) | 182, 410 |
| PfoI | TCCNGGA | 1 cut(s) | 426 |
| PkrI | GCNGC | 3 cut(s) | 255, 269, 483 |
| PleI | GAGTC | 2 cut(s) | 307, 535 |
| PpsI | GAGTC | 2 cut(s) | 307, 535 |
| PpuMI | RGGWCCY | 1 cut(s) | 498 |
| PshBI | ATTAAT | 1 cut(s) | 18 |
| Psp124BI | GAGCTC | 1 cut(s) | 395 |
| Psp5II | RGGWCCY | 1 cut(s) | 498 |
| Psp6I | CCWGG | 2 cut(s) | 312, 426 |
| PspGI | CCWGG | 2 cut(s) | 312, 426 |
| PspN4I | GGNNCC | 1 cut(s) | 500 |
| PspPI | GGNCC | 2 cut(s) | 451, 498 |
| PspPPI | RGGWCCY | 1 cut(s) | 498 |
| PspXI | VCTCGAGB | 3 cut(s) | 152, 257, 485 |
| PsuI | RGATCY | 2 cut(s) | 114, 195 |
| PsyI | GACNNNGTC | 2 cut(s) | 182, 410 |
| RsaI | GTAC | 1 cut(s) | 546 |
| RsaNI | GTAC | 1 cut(s) | 545 |
| SacI | GAGCTC | 1 cut(s) | 395 |
| SacII | CCGCGG | 1 cut(s) | 559 |
| SaqAI | TTAA | 2 cut(s) | 18, 59 |
| SatI | GCNGC | 3 cut(s) | 254, 268, 482 |
| Sau3AI | GATC | 3 cut(s) | 114, 195, 465 |
| Sau96I | GGNCC | 2 cut(s) | 451, 498 |
| SchI | GAGTC | 2 cut(s) | 308, 536 |
| ScrFI | CCNGG | 3 cut(s) | 314, 364, 428 |
| SduI | GDGCHC | 1 cut(s) | 395 |
| SetI | ASST | 9 cut(s) | 159, 167, 214, 258, 315, 395, 464, 478, 486 |
| SexAI | ACCWGGT | 1 cut(s) | 312 |
| SfaNI | GCATC | 1 cut(s) | 99 |
| Sfr274I | CTCGAG | 3 cut(s) | 152, 257, 485 |
| Sfr303I | CCGCGG | 1 cut(s) | 559 |
| SgrBI | CCGCGG | 1 cut(s) | 559 |
| SinI | GGWCC | 2 cut(s) | 451, 498 |
| SlaI | CTCGAG | 3 cut(s) | 152, 257, 485 |
| SmlI | CTYRAG | 4 cut(s) | 104, 152, 257, 485 |
| SmoI | CTYRAG | 4 cut(s) | 104, 152, 257, 485 |
| Sse9I | AATT | 1 cut(s) | 15 |
| SsiI | CCGC | 4 cut(s) | 268, 550, 556, 558 |
| SstI | GAGCTC | 1 cut(s) | 395 |
| StyD4I | CCNGG | 3 cut(s) | 312, 362, 426 |
| StyI | CCWWGG | 1 cut(s) | 5 |
| TaqI | TCGA | 3 cut(s) | 153, 258, 486 |
| TasI | AATT | 1 cut(s) | 15 |
| TatI | WGTACW | 1 cut(s) | 544 |
| TauI | GCSGC | 1 cut(s) | 270 |
| Tru1I | TTAA | 2 cut(s) | 18, 59 |
| Tru9I | TTAA | 2 cut(s) | 18, 59 |
| TseI | GCWGC | 2 cut(s) | 253, 481 |
| TspDTI | ATGAA | 1 cut(s) | 527 |
| TspGWI | ACGGA | 3 cut(s) | 69, 311, 317 |
| Tth111I | GACNNNGTC | 2 cut(s) | 182, 410 |
| VpaK11BI | GGWCC | 2 cut(s) | 451, 498 |
| VspI | ATTAAT | 1 cut(s) | 18 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 310 |
| XhoI | CTCGAG | 3 cut(s) | 152, 257, 485 |
| XmiI | GTMKAC | 1 cut(s) | 37 |
| XmnI | GAANNNNTTC | 1 cut(s) | 213 |
| Zsp2I | ATGCAT | 1 cut(s) | 58 |