pycom04g07010

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0020196
Physical Location & Seq
Reverse (-)
7309832 .. 7310982
1151 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGAGATTCCAAATAAGGATGTTGTGTCTTGGAATTCCATGGTTTCGGGGTATGCGAGGATGGGAAATATGGACAAGGCATATTTCTTGTTTCAACAAATTCCACAGAGGAGTTTGGCTTCTTGGAATAGGCATTTTTGATGCCATGCCGTTAAGAAATAATGTTTCTTGGATCACGATGATTAGCGGGTACTCAAAATGCGGCAATGTTGAGTCCGCTCGTCAACTCTTTGATCAGATGAGTGAAAGAGATGTGCTCTTGTTCAATGCCATGATAGCTTGCTATGCACAAAATAGCCAACCAAAGGAGGCCATTGAACTGTTCAACCAGATGCTTAAACTGGAAGTGAATATTCAACCAAATGAGACGACTTTGGCTAGTGTTATATCTGCTAGTTCTCAACTGGGAGATATGAAATTTGGGTTGTGGGCTGAGTTCTATATGAGTGAGCACGGAATTGAATTAGATGATCATTTGGCTACTGCTTTAGTTGATTTGTACACAAAATGCGGAGACATTGATAGGGCATATAAGTTGTTTCATGGCTTGAAAAAGAGAGATGTAGTTGCATATTCTGCAATGATCTTGGGATGCGGCATAAATGGTAAGGCAGTTGAAGCAACCAAGTTGTTTGAAGATATGGTAAATGCCGACATTTGCCCCAATGTGGTAACATACACTGGACTGCTAACTGCCTATAACCACGCCGGTCTAGTTGAAGAAGGCTACCGATGCTTTAATTCCATGAAAGAACATGGACTTGAGCCATCGGGCGATCATTATGGAATCATGGTCGACCTTTTAGGCAGAGCAGGACAGTTGGACAAAGCAGTTGAGCTAATAAGAAGCATGCCAATGCAGCCTCAAGCCGGGGTTTGGGGAGCTTTGCTTCTTGCTTGCTCATTACACAACAATGTTGAGCTTGGGGAGACTGCTGCTCGGAATTGCTTTAAGTTGGAGCCTGATAAAAACGGTTATTACGTTCTTCTTGCCAACATTTATGCTTCTGCTGCGAGATGGGACGATGAGAGGAGATTGAGAAAGGTCATGGAGAAGAAGGGGTTCGCCAAGATTCCAGGCTGTAGCTGGATGGATTCCATGTGA

Protein Analysis

368

Amino Acids

41.4

Weight (kDa)

6.32

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 53 - 85 5.7e-07 PPR repeat family
PPR PF01535 55 - 84 6.1e-08 PPR repeat
PPR_long PF17177 70 - 185 2.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 84 - 131 9e-09 PPR repeat family
PPR PF01535 88 - 114 1e-06 PPR repeat
PPR_2 PF13041 186 - 233 2.3e-11 PPR repeat family
PPR_long PF17177 190 - 281 3.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 209 - 269 8.9e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 221 - 266 1.9e-08 PPR repeat family
E_motif PF20431 303 - 364 1.2e-19 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020196)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22760
malus_domestica MD04G1078100.v1.1
prunus_persica Prupe.5G080600_v2.0.a1
pyrus_communis pycom04g07010 pycom06g06320 pycom06g06340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 218
AccI GTMKAC 1 cut(s) 795
AciI CCGC 5 cut(s) 186, 201, 216, 510, 594
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 3 cut(s) 33, 98, 416
AfaI GTAC 2 cut(s) 191, 500
AfiI CCNNNNNNNGG 4 cut(s) 304, 667, 869, 876
AjnI CCWGG 1 cut(s) 1075
AluBI AGCT 5 cut(s) 278, 838, 884, 922, 1086
AluI AGCT 5 cut(s) 278, 838, 884, 922, 1086
Alw21I GWGCWC 2 cut(s) 258, 453
Alw26I GTCTC 3 cut(s) 359, 507, 923
AlwI GGATC 1 cut(s) 179
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 3 cut(s) 859, 935, 1010
ApoI RAATTY 3 cut(s) 33, 98, 416
Asp700I GAANNNNTTC 1 cut(s) 1061
AsuC2I CCSGG 1 cut(s) 871
Bbv12I GWGCWC 2 cut(s) 258, 453
BbvI GCAGC 3 cut(s) 871, 922, 997
BccI CCATC 4 cut(s) 54, 775, 1011, 1084
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 1 cut(s) 1077
BclI TGATCA 2 cut(s) 232, 469
BcnI CCSGG 1 cut(s) 871
BcoDI GTCTC 3 cut(s) 359, 507, 923
BfaI CTAG 3 cut(s) 378, 393, 713
BfmI CTRYAG 1 cut(s) 1081
BisI GCNGC 5 cut(s) 202, 595, 860, 936, 1011
BlsI GCNGC 5 cut(s) 203, 596, 861, 937, 1012
Bme1390I CCNGG 2 cut(s) 871, 1077
BmiI GGNNCC 1 cut(s) 960
BmrFI CCNGG 2 cut(s) 871, 1077
BmrI ACTGGG 1 cut(s) 413
BmsI GCATC 4 cut(s) 130, 321, 581, 722
BmuI ACTGGG 1 cut(s) 413
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpuEI CTTGAG 2 cut(s) 782, 849
BpuMI CCSGG 1 cut(s) 871
BsaJI CCNNGG 2 cut(s) 38, 870
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 667, 869, 876
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 3 cut(s) 345, 408, 685
Bse3DI GCAATG 2 cut(s) 211, 585
BseBI CCWGG 1 cut(s) 1077
BseDI CCNNGG 2 cut(s) 38, 870
BseGI GGATG 4 cut(s) 24, 65, 596, 1095
BseLI CCNNNNNNNGG 4 cut(s) 304, 667, 869, 876
BseMI GCAATG 2 cut(s) 211, 585
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 3 cut(s) 345, 408, 685
BseRI GAGGAG 2 cut(s) 123, 1045
BseXI GCAGC 3 cut(s) 871, 922, 997
BshFI GGCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 258, 453
BsiSI CCGG 2 cut(s) 708, 870
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 4 cut(s) 304, 667, 869, 876
BsmAI GTCTC 3 cut(s) 359, 507, 923
BsmBI CGTCTC 1 cut(s) 359
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 258, 453
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 5 cut(s) 171, 232, 469, 582, 775
Bsp19I CCATGG 1 cut(s) 38
BspACI CCGC 5 cut(s) 186, 201, 216, 510, 594
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 960
BspPI GGATC 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 218
BsrDI GCAATG 2 cut(s) 211, 585
BsrFI RCCGGY 1 cut(s) 707
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 3 cut(s) 345, 408, 685
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 2 cut(s) 38, 870
BssMI GATC 5 cut(s) 171, 232, 469, 582, 775
BssT1I CCWWGG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 3 cut(s) 321, 819, 974
BstAPI GCANNNNNTGC 1 cut(s) 575
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 280, 851, 898
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 4 cut(s) 24, 65, 596, 1095
BstKTI GATC 5 cut(s) 174, 235, 472, 585, 778
BstMAI GTCTC 3 cut(s) 359, 507, 923
BstMBI GATC 5 cut(s) 171, 232, 469, 582, 775
BstMWI GCNNNNNNNGC 7 cut(s) 275, 284, 575, 617, 732, 859, 1010
BstNI CCWGG 1 cut(s) 1077
BstNSI RCATGY 1 cut(s) 853
BstSCI CCNGG 2 cut(s) 869, 1075
BstSFI CTRYAG 1 cut(s) 1081
BstV1I GCAGC 3 cut(s) 871, 922, 997
BsuRI GGCC 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 4 cut(s) 24, 65, 596, 1095
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 3 cut(s) 280, 851, 898
Cfr10I RCCGGY 1 cut(s) 707
Csp6I GTAC 2 cut(s) 190, 499
CviQI GTAC 2 cut(s) 190, 499
DdeI CTNAG 1 cut(s) 432
DpnI GATC 5 cut(s) 173, 234, 471, 584, 777
DpnII GATC 5 cut(s) 171, 232, 469, 582, 775
Eco130I CCWWGG 1 cut(s) 38
EcoRI GAATTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 1075
EcoT14I CCWWGG 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 359
FaqI GGGAC 1 cut(s) 1034
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 2 cut(s) 232, 469
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 5 cut(s) 202, 595, 860, 936, 1011
FokI GGATG 3 cut(s) 31, 72, 603
Fsp4HI GCNGC 5 cut(s) 202, 595, 860, 936, 1011
FspBI CTAG 3 cut(s) 378, 393, 713
GluI GCNGC 5 cut(s) 202, 595, 860, 936, 1011
HaeIII GGCC 1 cut(s) 311
HapII CCGG 2 cut(s) 708, 870
HincII GTYRAC 2 cut(s) 224, 796
HindII GTYRAC 2 cut(s) 224, 796
HinfI GANTC 5 cut(s) 6, 212, 786, 1072, 1094
HpaII CCGG 2 cut(s) 708, 870
Hpy166II GTNNAC 3 cut(s) 224, 501, 796
Hpy188I TCNGA 2 cut(s) 237, 942
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 3 cut(s) 224, 501, 796
HpyAV CCTTC 2 cut(s) 716, 1051
HpyCH4III ACNGT 3 cut(s) 321, 819, 974
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 4 cut(s) 287, 569, 578, 859
HpyF10VI GCNNNNNNNGC 7 cut(s) 275, 284, 575, 617, 732, 859, 1010
HpyF3I CTNAG 1 cut(s) 432
HpySE526I ACGT 1 cut(s) 981
Ksp22I TGATCA 2 cut(s) 232, 469
Kzo9I GATC 5 cut(s) 171, 232, 469, 582, 775
LmnI GCTCC 2 cut(s) 881, 958
Lsp1109I GCAGC 3 cut(s) 871, 922, 997
LweI GCATC 4 cut(s) 130, 321, 581, 722
MaeI CTAG 3 cut(s) 378, 393, 713
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 1 cut(s) 670
MalI GATC 5 cut(s) 173, 234, 471, 584, 777
MbiI CCGCTC 1 cut(s) 218
MboI GATC 5 cut(s) 171, 232, 469, 582, 775
MboII GAAGA 4 cut(s) 647, 731, 977, 1066
MhlI GDGCHC 2 cut(s) 258, 453
MluCI AATT 7 cut(s) 33, 98, 416, 456, 461, 739, 943
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 2 cut(s) 801, 936
MnlI CCTC 5 cut(s) 50, 101, 301, 873, 1023
MroXI GAANNNNTTC 1 cut(s) 1061
MseI TTAA 4 cut(s) 152, 336, 738, 951
MslI CAYNNNNRTG 2 cut(s) 854, 912
MspI CCGG 2 cut(s) 708, 870
MspR9I CCNGG 2 cut(s) 871, 1077
MvaI CCWGG 1 cut(s) 1077
MwoI GCNNNNNNNGC 7 cut(s) 275, 284, 575, 617, 732, 859, 1010
NciI CCSGG 1 cut(s) 871
NcoI CCATGG 1 cut(s) 38
NdeII GATC 5 cut(s) 171, 232, 469, 582, 775
NlaIV GGNNCC 1 cut(s) 960
NspI RCATGY 1 cut(s) 853
PaeI GCATGC 1 cut(s) 853
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 978
PdmI GAANNNNTTC 1 cut(s) 1061
PfeI GAWTC 4 cut(s) 6, 786, 1072, 1094
PkrI GCNGC 5 cut(s) 203, 596, 861, 937, 1012
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 1 cut(s) 960
RsaI GTAC 2 cut(s) 191, 500
RsaNI GTAC 2 cut(s) 190, 499
RseI CAYNNNNRTG 2 cut(s) 854, 912
SalI GTCGAC 1 cut(s) 794
SaqAI TTAA 4 cut(s) 152, 336, 738, 951
SatI GCNGC 5 cut(s) 202, 595, 860, 936, 1011
Sau3AI GATC 5 cut(s) 171, 232, 469, 582, 775
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 2 cut(s) 871, 1077
SduI GDGCHC 2 cut(s) 258, 453
SetI ASST 8 cut(s) 280, 801, 840, 886, 924, 984, 1047, 1088
SfaNI GCATC 4 cut(s) 130, 321, 581, 722
SfcI CTRYAG 1 cut(s) 1081
SmiMI CAYNNNNRTG 2 cut(s) 854, 912
SmlI CTYRAG 2 cut(s) 761, 864
SmoI CTYRAG 2 cut(s) 761, 864
SphI GCATGC 1 cut(s) 853
Sse9I AATT 7 cut(s) 33, 98, 416, 456, 461, 739, 943
SsiI CCGC 5 cut(s) 186, 201, 216, 510, 594
SspI AATATT 1 cut(s) 352
SspMI CTAG 3 cut(s) 378, 393, 713
StyD4I CCNGG 2 cut(s) 869, 1075
StyI CCWWGG 1 cut(s) 38
TaaI ACNGT 3 cut(s) 321, 819, 974
TaiI ACGT 1 cut(s) 984
TaqI TCGA 1 cut(s) 795
TasI AATT 7 cut(s) 33, 98, 416, 456, 461, 739, 943
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 204, 597
TfiI GAWTC 4 cut(s) 6, 786, 1072, 1094
Tru1I TTAA 4 cut(s) 152, 336, 738, 951
Tru9I TTAA 4 cut(s) 152, 336, 738, 951
TscAI CASTG 1 cut(s) 685
TseI GCWGC 3 cut(s) 859, 935, 1010
TspDTI ATGAA 3 cut(s) 428, 530, 761
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 685
XapI RAATTY 3 cut(s) 33, 98, 416
XceI RCATGY 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 1083
XmiI GTMKAC 1 cut(s) 795
XmnI GAANNNNTTC 1 cut(s) 1061
XspI CTAG 3 cut(s) 378, 393, 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.