pycom04g08680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0020366
Physical Location & Seq
Reverse (-)
10597524 .. 10599042
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g08680.1

Sequence Viewer

Length: 1149 bp
ATGGTTGACCCTATTCATCAATATTCAATCATCTCTATTTACATCACTATTCAATCATCACCTTACCCATCATTTTTTCAATTAACACAGATTTTTGAGACCATCCCTCTCCACAAAACTAGCCACCCTATAAGAGCAAGCCCAGCGTGGGCTATAGCTCGGGGGACAGTGGACAGAACAAGGGCCCGGGGGCTGGTGGGTCAACTCCAGCGTGGGCTAGCCCGAACGAAGGTGGAGCCTGAGGGCCATGTGACGTCAGCCTGGCGTCAGGCAATATTAAATTTTTTTTGTGTCAGGCACGTGCACCCCACTCGCGCGTGGGGGCGCGTCGCTGACACAAAATCAGAAAAAAAAAAAAACCTTTGTCACTCTCAACGGTTATTTAATTTGGACCGTTCGATCTCAACGATCAAAAAAAATAAAAAAGTGTTATTTAATATCCACCGTTCGATCCAAGATCAACGGTGGATATTAAAATGCTTTATAAATAAAAAAATAAAAGAAAAAACAGTTATAAAATTAAGAAAAGTTACCGTTGTGACACGTGGCACAATCTGGAGTGTTGGAATTCAAATTTTTTTATATCCAACGTCAAAAAAATTCAAAAAAAAAAAATTTGAAAAATCTGAAAAAAATTGTAAAAAAAATTGTTTTTACTTTTCTATAAATACCTTCTTATTATCATCTACCTTACACAACAATTTCATATTTTCTCAACTACTTTCAATCACATTCCTTTCTTTGTCTCAAAGTTTGAATCCATTTTTTTTTCAACAAAATGACCACTGGTGCAGGTACGAATTGGTCTTTCTCTTATTGAAGATGTTGCGTTGTGTACTATCTGGGTTGAAGTTACTCATAGTTCGCTTACGGGTAATGAGATGCAGTTGCGAGAAATGTGGAGTCTTATTCATACCAATTTTCTTGAGAAAATTGGTGGGAAAAGAACCAAAGAATCGATATCCAGTCGTTGAAAATTACTTAGCCAATCGTTTAGTACGTGGAGAGACACCTTGGCACAAGCTAGTAGTAATATTCAAAGTGGGGAAAATTACTCGGATCAGTATAATTATGTTGTTTGTATTATTTATTTGTTACCTACATAATTTATTATTTATTGTTTGCATTATTTATTTTTTCATTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

383

Amino Acids

44.85

Weight (kDa)

10.36

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020366)

Species Orthologous Gene IDs
malus_domestica MD03G1066700.v1.1
pyrus_communis pycom04g08680 pycom06g21610 pycom11g02830 pycom15g17860 pycom15g29930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 485, 515
AatII GACGTC 1 cut(s) 257
Acc36I ACCTGC 1 cut(s) 783
AccII CGCG 3 cut(s) 315, 317, 327
AclWI GGATC 2 cut(s) 445, 1067
AcsI RAATTY 5 cut(s) 280, 567, 573, 599, 614
AcvI CACGTG 2 cut(s) 301, 545
AcyI GRCGYC 2 cut(s) 254, 265
AfaI GTAC 3 cut(s) 797, 837, 999
AfiI CCNNNNNNNGG 3 cut(s) 148, 193, 229
AflIII ACRYGT 1 cut(s) 542
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 2 cut(s) 158, 1024
AluI AGCT 2 cut(s) 158, 1024
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 3 cut(s) 92, 750, 1001
Alw44I GTGCAC 1 cut(s) 302
AlwI GGATC 2 cut(s) 445, 1067
Ama87I CYCGRG 2 cut(s) 159, 186
AoxI GGCC 2 cut(s) 183, 244
ApaI GGGCCC 1 cut(s) 187
ApaLI GTGCAC 1 cut(s) 302
ApoI RAATTY 5 cut(s) 280, 567, 573, 599, 614
AspLEI GCGC 2 cut(s) 317, 327
AspS9I GGNCC 4 cut(s) 183, 184, 244, 391
AsuC2I CCSGG 2 cut(s) 187, 188
AsuHPI GGTGA 1 cut(s) 51
AsuNHI GCTAGC 1 cut(s) 217
AvaI CYCGRG 2 cut(s) 159, 186
AvaII GGWCC 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 240
BaeGI GKGCMC 2 cut(s) 187, 306
BanII GRGCYC 1 cut(s) 187
BbrPI CACGTG 2 cut(s) 301, 545
Bbv12I GWGCWC 1 cut(s) 306
BccI CCATC 2 cut(s) 76, 110
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 2 cut(s) 187, 188
BcoDI GTCTC 3 cut(s) 92, 750, 1001
BfaI CTAG 3 cut(s) 120, 218, 1025
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 1 cut(s) 783
Bme1390I CCNGG 3 cut(s) 187, 188, 262
Bme18I GGWCC 1 cut(s) 391
BmeT110I CYCGRG 2 cut(s) 159, 186
BmgT120I GGNCC 4 cut(s) 183, 184, 244, 391
BmiI GGNNCC 2 cut(s) 185, 237
BmrFI CCNGG 3 cut(s) 187, 188, 262
BmsI GCATC 1 cut(s) 872
BmtI GCTAGC 1 cut(s) 221
BpmI CTGGAG 2 cut(s) 191, 577
BpuEI CTTGAG 1 cut(s) 946
BpuMI CCSGG 2 cut(s) 187, 188
Bsa29I ATCGAT 1 cut(s) 958
BsaAI YACGTR 3 cut(s) 301, 545, 1001
BsaHI GRCGYC 2 cut(s) 254, 265
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 186, 187, 1013
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 193, 229
Bse1I ACTGG 2 cut(s) 791, 965
Bse21I CCTNAGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 262
BseCI ATCGAT 1 cut(s) 958
BseDI CCNNGG 3 cut(s) 186, 187, 1013
BseGI GGATG 1 cut(s) 102
BseLI CCNNNNNNNGG 3 cut(s) 148, 193, 229
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 791, 965
BseSI GKGCMC 2 cut(s) 187, 306
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 811
Bsh1236I CGCG 3 cut(s) 315, 317, 327
BshFI GGCC 2 cut(s) 185, 246
BshVI ATCGAT 1 cut(s) 958
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 159, 186
BsiSI CCGG 1 cut(s) 187
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 3 cut(s) 148, 193, 229
BsmAI GTCTC 3 cut(s) 92, 750, 1001
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 2 cut(s) 185, 246
Bso31I GGTCTC 1 cut(s) 92
BsoBI CYCGRG 2 cut(s) 159, 186
Bsp120I GGGCCC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 187, 306
Bsp143I GATC 5 cut(s) 399, 408, 450, 457, 1059
BspANI GGCC 2 cut(s) 185, 246
BspCNI CTCAG 1 cut(s) 232
BspDI ATCGAT 1 cut(s) 958
BspFNI CGCG 3 cut(s) 315, 317, 327
BspLI GGNNCC 2 cut(s) 185, 237
BspMI ACCTGC 1 cut(s) 783
BspOI GCTAGC 1 cut(s) 221
BspPI GGATC 2 cut(s) 445, 1067
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 2 cut(s) 791, 965
BssECI CCNNGG 3 cut(s) 186, 187, 1013
BssMI GATC 5 cut(s) 399, 408, 450, 457, 1059
BssNI GRCGYC 2 cut(s) 254, 265
BssT1I CCWWGG 1 cut(s) 1013
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 7 cut(s) 169, 378, 395, 446, 465, 511, 535
BstACI GRCGYC 2 cut(s) 254, 265
BstBAI YACGTR 3 cut(s) 301, 545, 1001
BstC8I GCNNGC 2 cut(s) 139, 219
BstDEI CTNAG 2 cut(s) 240, 982
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 3 cut(s) 315, 317, 327
BstHHI GCGC 2 cut(s) 317, 327
BstKTI GATC 5 cut(s) 402, 411, 453, 460, 1062
BstMAI GTCTC 3 cut(s) 92, 750, 1001
BstMBI GATC 5 cut(s) 399, 408, 450, 457, 1059
BstMWI GCNNNNNNNGC 1 cut(s) 143
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 185, 186, 260
BstSFI CTRYAG 1 cut(s) 153
BstSLI GKGCMC 2 cut(s) 187, 306
BstUI CGCG 3 cut(s) 315, 317, 327
Bsu15I ATCGAT 1 cut(s) 958
Bsu36I CCTNAGG 1 cut(s) 240
BsuRI GGCC 2 cut(s) 185, 246
BsuTUI ATCGAT 1 cut(s) 958
BtsCI GGATG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 174, 784
BveI ACCTGC 1 cut(s) 783
Cac8I GCNNGC 2 cut(s) 139, 219
CfoI GCGC 2 cut(s) 317, 327
Cfr13I GGNCC 4 cut(s) 183, 184, 244, 391
Cfr9I CCCGGG 1 cut(s) 186
ClaI ATCGAT 1 cut(s) 958
CseI GACGC 2 cut(s) 254, 316
Csp6I GTAC 3 cut(s) 796, 836, 998
CviAII CATG 1 cut(s) 248
CviQI GTAC 3 cut(s) 796, 836, 998
DdeI CTNAG 2 cut(s) 240, 982
DpnI GATC 5 cut(s) 401, 410, 452, 459, 1061
DpnII GATC 5 cut(s) 399, 408, 450, 457, 1059
Eco130I CCWWGG 1 cut(s) 1013
Eco24I GRGCYC 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 962
Eco47I GGWCC 1 cut(s) 391
Eco72I CACGTG 2 cut(s) 301, 545
Eco81I CCTNAGG 1 cut(s) 240
Eco88I CYCGRG 2 cut(s) 159, 186
EcoO109I RGGNCCY 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 1 cut(s) 260
EcoRV GATATC 1 cut(s) 962
EcoT14I CCWWGG 1 cut(s) 1013
EcoT38I GRGCYC 1 cut(s) 187
ErhI CCWWGG 1 cut(s) 1013
FaeI CATG 1 cut(s) 251
FaqI GGGAC 1 cut(s) 178
FatI CATG 1 cut(s) 247
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 187
FspBI CTAG 3 cut(s) 120, 218, 1025
GlaI GCGC 2 cut(s) 316, 326
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 2 cut(s) 191, 577
HaeIII GGCC 2 cut(s) 185, 246
HapII CCGG 1 cut(s) 187
HgaI GACGC 2 cut(s) 254, 316
HhaI GCGC 2 cut(s) 317, 327
Hin1I GRCGYC 2 cut(s) 254, 265
Hin1II CATG 1 cut(s) 251
Hin6I GCGC 2 cut(s) 315, 325
HinP1I GCGC 2 cut(s) 315, 325
HincII GTYRAC 2 cut(s) 7, 203
HindII GTYRAC 2 cut(s) 7, 203
HinfI GANTC 3 cut(s) 757, 903, 955
HpaII CCGG 1 cut(s) 187
HphI GGTGA 1 cut(s) 51
Hpy166II GTNNAC 5 cut(s) 7, 172, 203, 304, 836
Hpy188I TCNGA 3 cut(s) 346, 628, 1059
Hpy188III TCNNGA 2 cut(s) 556, 925
Hpy8I GTNNAC 5 cut(s) 7, 172, 203, 304, 836
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 2 cut(s) 223, 682
HpyCH4III ACNGT 7 cut(s) 169, 378, 395, 446, 465, 511, 535
HpyCH4IV ACGT 5 cut(s) 254, 300, 544, 590, 1000
HpyCH4V TGCA 4 cut(s) 304, 792, 885, 1125
HpyF10VI GCNNNNNNNGC 1 cut(s) 143
HpyF3I CTNAG 2 cut(s) 240, 982
HpySE526I ACGT 5 cut(s) 254, 300, 544, 590, 1000
Hsp92I GRCGYC 2 cut(s) 254, 265
Hsp92II CATG 1 cut(s) 251
HspAI GCGC 2 cut(s) 315, 325
Kzo9I GATC 5 cut(s) 399, 408, 450, 457, 1059
LmnI GCTCC 1 cut(s) 235
LweI GCATC 1 cut(s) 872
MaeI CTAG 3 cut(s) 120, 218, 1025
MaeII ACGT 5 cut(s) 254, 300, 544, 590, 1000
MaeIII GTNAC 6 cut(s) 250, 365, 529, 538, 852, 1094
MalI GATC 5 cut(s) 401, 410, 452, 459, 1061
MboI GATC 5 cut(s) 399, 408, 450, 457, 1059
MboII GAAGA 1 cut(s) 832
MhlI GDGCHC 2 cut(s) 187, 306
MlyI GAGTC 1 cut(s) 912
MmeI TCCRAC 2 cut(s) 544, 611
MnlI CCTC 2 cut(s) 117, 235
MseI TTAA 6 cut(s) 83, 278, 384, 435, 473, 521
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 3 cut(s) 187, 188, 262
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 3 cut(s) 315, 317, 327
MwoI GCNNNNNNNGC 1 cut(s) 143
NciI CCSGG 2 cut(s) 187, 188
NdeII GATC 5 cut(s) 399, 408, 450, 457, 1059
NheI GCTAGC 1 cut(s) 217
NlaIII CATG 1 cut(s) 251
NlaIV GGNNCC 2 cut(s) 185, 237
NmuCI GTSAC 3 cut(s) 250, 365, 538
PcsI WCGNNNNNNNCGW 2 cut(s) 404, 997
PfeI GAWTC 2 cut(s) 757, 955
PleI GAGTC 1 cut(s) 911
PmaCI CACGTG 2 cut(s) 301, 545
PmlI CACGTG 2 cut(s) 301, 545
PpsI GAGTC 1 cut(s) 911
Ppu21I YACGTR 3 cut(s) 301, 545, 1001
PsiI TTATAA 2 cut(s) 485, 515
Psp6I CCWGG 1 cut(s) 260
PspCI CACGTG 2 cut(s) 301, 545
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 185, 237
PspOMI GGGCCC 1 cut(s) 183
PspPI GGNCC 4 cut(s) 183, 184, 244, 391
RsaI GTAC 3 cut(s) 797, 837, 999
RsaNI GTAC 3 cut(s) 796, 836, 998
SaqAI TTAA 6 cut(s) 83, 278, 384, 435, 473, 521
Sau3AI GATC 5 cut(s) 399, 408, 450, 457, 1059
Sau96I GGNCC 4 cut(s) 183, 184, 244, 391
SchI GAGTC 1 cut(s) 912
ScrFI CCNGG 3 cut(s) 187, 188, 262
SduI GDGCHC 2 cut(s) 187, 306
SfaNI GCATC 1 cut(s) 872
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 1 cut(s) 391
SmaI CCCGGG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 925
SmoI CTYRAG 1 cut(s) 925
SspI AATATT 3 cut(s) 23, 276, 1035
SspMI CTAG 3 cut(s) 120, 218, 1025
StyD4I CCNGG 3 cut(s) 185, 186, 260
StyI CCWWGG 1 cut(s) 1013
TaaI ACNGT 7 cut(s) 169, 378, 395, 446, 465, 511, 535
TaiI ACGT 5 cut(s) 257, 303, 547, 593, 1003
TaqI TCGA 3 cut(s) 398, 449, 958
TatI WGTACW 1 cut(s) 835
TfiI GAWTC 2 cut(s) 757, 955
Tru1I TTAA 6 cut(s) 83, 278, 384, 435, 473, 521
Tru9I TTAA 6 cut(s) 83, 278, 384, 435, 473, 521
TscAI CASTG 2 cut(s) 174, 791
TseFI GTSAC 3 cut(s) 250, 365, 538
Tsp45I GTSAC 3 cut(s) 250, 365, 538
TspDTI ATGAA 4 cut(s) 5, 694, 901, 1129
TspMI CCCGGG 1 cut(s) 186
TspRI CASTG 2 cut(s) 174, 791
VneI GTGCAC 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 5 cut(s) 280, 567, 573, 599, 614
XmaI CCCGGG 1 cut(s) 186
XspI CTAG 3 cut(s) 120, 218, 1025
ZraI GACGTC 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.