Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0011071

Physical Location & Seq

Genomic Coordinates Reverse (-)

12123829 .. 12128257

Gene Structure 4429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1554 bp

ATGATCAGTACGGCCAACGGTATGATGCAAACGGCGTCGTCTAGCAACGCTCAGTCGCCGGGTCTCAAGACCTATTTCAAAACCCCTGAGGGAAAGTACAAGCTTCAGTACGAGAAGACGCATCCTTCGGGGCCCCTTCACTACACCCACGGCAAAACCGTCACTCAGGCAACATTGGCAAATCTTAAGGATAAACCTGCTCCATCAACCCCGACCGCGCCATCTTCAAGCTTCAGCACTGGAGGTGGCGTACGTTCAGCTGCAGCAAGGTTGTTGGGTGGTGGTAATGGGACCCGGGCGCTTAGCTTTGTTGCAGGGAATGGAGGAGGTAAGAGTGTCAGTGCGAGTAGTAGAGTTGGGTCTTTGGTGGCTTCCAGTTCGAGTACTTCGACTTCTACTTCGAATTTTGATGGGAAAGGGAATTATTTGATCTTCAATGTGGGGGATGCGATCTTCATCAGTGATTTGAATTCTCAAGACAAGGATCCAATAAAGTCTATACATTTCAGTAATTCAAACCCTGTCTGCCACGCATTTGATCAAGATGCGAAGGATGGTCATGATTTGATTATTGGGTTGAGTTGCGGTGATGTCTACTCAGTGTCACTGAGACAGCAATTGCAGGATGTTGGAAAGAAGCTTGTAGGTGCTCAGCATTATAACAAAGATGGCTCTGTTAGTAACAGCCGTTGTACTAGTATTGCATGGGTTCCTGGTGGTGACGGTGCTTTTGTTGTCGCTTATGCCGATGGGAATCTGTATGTATATGAAAAGAGCAAAGATGGGGCAGGCGATTCCTCATTCCCTGTTATCAAAGATCCAGCTCAATTTTCAGTTTCGCATGCACGTTATAATAAGAGTAACCCAACTGCCAGATGGCATATCTGCCAAGGTTCAGTAAATAGTATTGCTTTCTCGACTGATGGGTCCTATTTAGCGACTGTTGGAAGAGATGGTTATCTACGTGTTTTTGACTACTCAAAACAGCAACTTATATGTGGTGGCAAAAGTTACTATGGGGCCCTTTTATGTTGCACTTGGAGTATGGATGGAAAATACATACTGACAGGAGGTGAAGATGATCGAGTTCAAGTTTGGAGCATGGAAGATCGGAAAGTTGTAGCATGGGGAGAGGGGCACAACTCTTGGGTTAGTGGAGTGGCGTTTGATTCGTATTGGTCGTCACCAAATTCAGATGGTATGGGGGAGACTGTCATGTACCGGTTTGGCTCTGTCGGTCAGGACACACAGTTGCTTCTGTGGGACCTGGAAATGGATGAGATTGTGGTGCCATTGCGGCGATGTCCTCCTGGCGGCTCCCCCACATATAGTGCTGGAAGCCAGTCATCTCATTGGGACAATGCACTTCCAGTAGGTACTCTGCAGCCTGCTCCAAGCTTGCGAGATGTTCCAAAATTATCACCGCTGGTTGCTCACCGGGTACACACTGAACCCCTCTCTGGCGTGATGTTTACCCAAGAATCCGTTCTTACTGTCTGCCGAGACGGGCATATAAAAGTTTGGGTGAGACCTGGGGTTTCAGAAACCCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006355 GO:0007154 GO:0007584 GO:0008150 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0042221 GO:0045013 GO:0045892 GO:0045934 GO:0045990 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061984 GO:0061985 GO:0065007 GO:0070887 GO:0071496 GO:0080090 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

518

Amino Acids

55.24

Weight (kDa)

7.2

Isoelectric Point (pI)

41.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_RIG_2nd	PF23775	141 - 358	1e-05	RIG second beta-propeller
Beta-prop_Nup120_160	PF11715	166 - 333	6.5e-07	Nucleoporin Nup120/160, beta-propeller domain
Beta-prop_WDR3_1st	PF25173	285 - 428	1.4e-12	WDR3 first beta-propeller domain
WDR55	PF24796	285 - 390	7.3e-08	WDR55
Beta-prop_TEP1_2nd	PF25047	294 - 388	2e-08	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	297 - 400	4e-14	WDR5 beta-propeller domain
WD40	PF00400	297 - 325	5.9e-06	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	303 - 390	1.1e-07	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	344 - 427	1.9e-06	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0011071)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37160 AT2G37160 AT3G53390
fragaria_vesca	FvH4_6g15710 FvH4_6g15710
malus_domestica	MD04G1099900.v1.1 MD12G1120000.v1.1
prunus_persica	Prupe.6G238200_v2.0.a1
pyrus_communis	pycom04g09500 pycom12g11890
rosa_chinensis	RchiOBHm_Chr3g0469411
rosa_laevigata	RLG00000024351
rosa_multiflora	Rmu_sc0005274.1_g000009
rosa_roxburghii	Rroxscaffold_6G00411910
rosa_rugosa	Rorug03G0101600
rosa_samantha	Rh3AG150600 Rh3BG174300 Rh3CG165300 Rh3DG201100
rosa_wichuraiana	Rw3G014200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	660, 852
AasI	GACNNNNNNGTC	1 cut(s)	925
Acc36I	ACCTGC	1 cut(s)	205
AccB1I	GGYRCC	1 cut(s)	1288
AccI	GTMKAC	1 cut(s)	594
AccII	CGCG	1 cut(s)	218
AciI	CCGC	5 cut(s)	216, 585, 1297, 1314, 1424
AclWI	GGATC	3 cut(s)	479, 492, 812
AcoI	YGGCCR	1 cut(s)	12
AcsI	RAATTY	3 cut(s)	403, 469, 1189
AcuI	CTGAAG	2 cut(s)	89, 217
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	9 cut(s)	10, 98, 110, 252, 385, 694, 1220, 1378, 1443
AfiI	CCNNNNNNNGG	3 cut(s)	1273, 1313, 1460
AflII	CTTAAG	1 cut(s)	185
AflIII	ACRYGT	1 cut(s)	964
AgeI	ACCGGT	1 cut(s)	1221
AgsI	TTSAA	6 cut(s)	79, 228, 436, 469, 516, 1091
AhlI	ACTAGT	1 cut(s)	695
AjnI	CCWGG	4 cut(s)	712, 1266, 1309, 1531
AjuI	GAANNNNNNNTTGG	2 cut(s)	1470, 1502
AluBI	AGCT	7 cut(s)	103, 231, 260, 306, 640, 824, 1398
AluI	AGCT	7 cut(s)	103, 231, 260, 306, 640, 824, 1398
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	5 cut(s)	68, 604, 1202, 1497, 1522
AlwI	GGATC	3 cut(s)	479, 492, 812
Ama87I	CYCGRG	1 cut(s)	294
AoxI	GGCC	3 cut(s)	12, 131, 1020
ApaI	GGGCCC	2 cut(s)	135, 1024
ApeKI	GCWGC	3 cut(s)	260, 263, 1384
ApoI	RAATTY	3 cut(s)	403, 469, 1189
ArsI	GACNNNNNNTTYG	6 cut(s)	109, 141, 382, 414, 713, 745
AsiGI	ACCGGT	1 cut(s)	1221
Asp700I	GAANNNNTTC	1 cut(s)	1485
AspLEI	GCGC	2 cut(s)	220, 301
AspS9I	GGNCC	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
AsuC2I	CCSGG	4 cut(s)	60, 295, 296, 1439
AsuHPI	GGTGA	7 cut(s)	599, 731, 1085, 1176, 1413, 1427, 1537
AsuII	TTCGAA	1 cut(s)	401
AvaI	CYCGRG	1 cut(s)	294
AvaII	GGWCC	3 cut(s)	291, 927, 1264
AxyI	CCTNAGG	1 cut(s)	87
BaeGI	GKGCMC	3 cut(s)	135, 1024, 1140
BamHI	GGATCC	1 cut(s)	484
BanI	GGYRCC	1 cut(s)	1288
BanII	GRGCYC	2 cut(s)	135, 1024
BarI	GAAGNNNNNNTAC	2 cut(s)	376, 408
BbsI	GAAGAC	1 cut(s)	122
Bbv12I	GWGCWC	1 cut(s)	652
BbvI	GCAGC	3 cut(s)	247, 275, 1396
BceAI	ACGGC	4 cut(s)	27, 48, 166, 672
BciT130I	CCWGG	4 cut(s)	714, 1268, 1311, 1533
BclI	TGATCA	2 cut(s)	3, 538
BcnI	CCSGG	4 cut(s)	60, 295, 296, 1439
BcoDI	GTCTC	5 cut(s)	68, 604, 1202, 1497, 1522
BcuI	ACTAGT	1 cut(s)	695
BfaI	CTAG	2 cut(s)	42, 696
BfmI	CTRYAG	2 cut(s)	261, 1382
BfoI	RGCGCY	1 cut(s)	302
BfrI	CTTAAG	1 cut(s)	185
BfuAI	ACCTGC	1 cut(s)	205
BglI	GCCNNNNNGGC	1 cut(s)	1297
BisI	GCNGC	5 cut(s)	261, 264, 1298, 1315, 1385
BlpI	GCTNAGC	2 cut(s)	302, 651
BlsI	GCNGC	5 cut(s)	262, 265, 1299, 1316, 1386
BmcAI	AGTACT	1 cut(s)	385
Bme1390I	CCNGG	8 cut(s)	60, 295, 296, 714, 1268, 1311, 1439, 1533
Bme18I	GGWCC	3 cut(s)	291, 927, 1264
BmeT110I	CYCGRG	1 cut(s)	294
BmgT120I	GGNCC	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
BmrFI	CCNGG	8 cut(s)	60, 295, 296, 714, 1268, 1311, 1439, 1533
BmsI	GCATC	4 cut(s)	15, 130, 436, 535
BpiI	GAAGAC	1 cut(s)	122
BpmI	CTGGAG	1 cut(s)	261
Bpu1102I	GCTNAGC	2 cut(s)	302, 651
Bpu14I	TTCGAA	1 cut(s)	401
BpuEI	CTTGAG	2 cut(s)	50, 459
BpuMI	CCSGG	4 cut(s)	60, 295, 296, 1439
BsaAI	YACGTR	1 cut(s)	965
BsaBI	GATNNNNATC	3 cut(s)	455, 753, 957
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	2 cut(s)	68, 1522
BsaJI	CCNNGG	4 cut(s)	148, 294, 889, 1532
BsaWI	WCCGGW	1 cut(s)	1221
BsaXI	ACNNNNNCTCC	2 cut(s)	234, 264
Bsc4I	CCNNNNNNNGG	3 cut(s)	1273, 1313, 1460
Bse118I	RCCGGY	1 cut(s)	1221
Bse1I	ACTGG	4 cut(s)	244, 375, 1342, 1370
Bse21I	CCTNAGG	1 cut(s)	87
Bse3DI	GCAATG	1 cut(s)	1292
Bse8I	GATNNNNATC	3 cut(s)	455, 753, 957
BseBI	CCWGG	4 cut(s)	714, 1268, 1311, 1533
BseDI	CCNNGG	4 cut(s)	148, 294, 889, 1532
BseGI	GGATG	6 cut(s)	121, 451, 559, 631, 1054, 1282
BseJI	GATNNNNATC	3 cut(s)	455, 753, 957
BseLI	CCNNNNNNNGG	3 cut(s)	1273, 1313, 1460
BseMI	GCAATG	1 cut(s)	1292
BseMII	CTCAG	6 cut(s)	65, 78, 179, 599, 612, 665
BseNI	ACTGG	4 cut(s)	244, 375, 1342, 1370
BseRI	GAGGAG	1 cut(s)	339
BseSI	GKGCMC	3 cut(s)	135, 1024, 1140
BseXI	GCAGC	3 cut(s)	247, 275, 1396
Bsh1236I	CGCG	1 cut(s)	218
Bsh1285I	CGRYCG	1 cut(s)	216
BshFI	GGCC	3 cut(s)	14, 133, 1022
BshNI	GGYRCC	1 cut(s)	1288
BshTI	ACCGGT	1 cut(s)	1221
BsiEI	CGRYCG	1 cut(s)	216
BsiHKAI	GWGCWC	1 cut(s)	652
BsiHKCI	CYCGRG	1 cut(s)	294
BsiSI	CCGG	4 cut(s)	59, 295, 1222, 1438
BsiWI	CGTACG	1 cut(s)	250
BslFI	GGGAC	3 cut(s)	304, 1277, 1370
BslI	CCNNNNNNNGG	3 cut(s)	1273, 1313, 1460
BsmAI	GTCTC	5 cut(s)	68, 604, 1202, 1497, 1522
BsmBI	CGTCTC	1 cut(s)	1497
BsmFI	GGGAC	3 cut(s)	304, 1277, 1370
BsnI	GGCC	3 cut(s)	14, 133, 1022
Bso31I	GGTCTC	2 cut(s)	68, 1522
BsoBI	CYCGRG	1 cut(s)	294
Bsp119I	TTCGAA	1 cut(s)	401
Bsp120I	GGGCCC	2 cut(s)	131, 1020
Bsp1286I	GDGCHC	4 cut(s)	135, 652, 1024, 1140
Bsp143I	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
Bsp1720I	GCTNAGC	2 cut(s)	302, 651
BspACI	CCGC	5 cut(s)	216, 585, 1297, 1314, 1424
BspANI	GGCC	3 cut(s)	14, 133, 1022
BspCNI	CTCAG	6 cut(s)	64, 79, 178, 600, 611, 664
BspFNI	CGCG	1 cut(s)	218
BspHI	TCATGA	1 cut(s)	559
BspMAI	CTGCAG	2 cut(s)	265, 1386
BspMI	ACCTGC	1 cut(s)	205
BspPI	GGATC	3 cut(s)	479, 492, 812
BspT104I	TTCGAA	1 cut(s)	401
BspT107I	GGYRCC	1 cut(s)	1288
BspTI	CTTAAG	1 cut(s)	185
BspTNI	GGTCTC	2 cut(s)	68, 1522
BsrDI	GCAATG	1 cut(s)	1292
BsrFI	RCCGGY	1 cut(s)	1221
BsrI	ACTGG	4 cut(s)	244, 375, 1342, 1370
BssAI	RCCGGY	1 cut(s)	1221
BssECI	CCNNGG	4 cut(s)	148, 294, 889, 1532
BssMI	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	1 cut(s)	889
Bst2UI	CCWGG	4 cut(s)	714, 1268, 1311, 1533
Bst4CI	ACNGT	7 cut(s)	20, 160, 725, 943, 1213, 1251, 1495
Bst6I	CTCTTC	1 cut(s)	943
BstACI	GRCGYC	1 cut(s)	35
BstAFI	CTTAAG	1 cut(s)	185
BstBAI	YACGTR	1 cut(s)	965
BstBI	TTCGAA	1 cut(s)	401
BstC8I	GCNNGC	4 cut(s)	790, 843, 1389, 1400
BstDEI	CTNAG	7 cut(s)	51, 87, 165, 302, 598, 608, 651
BstDSI	CCRYGG	1 cut(s)	148
BstF5I	GGATG	6 cut(s)	121, 451, 559, 631, 1054, 1282
BstFNI	CGCG	1 cut(s)	218
BstH2I	RGCGCY	1 cut(s)	302
BstHHI	GCGC	2 cut(s)	220, 301
BstKTI	GATC	8 cut(s)	6, 432, 453, 487, 541, 820, 1084, 1111
BstMAI	GTCTC	5 cut(s)	68, 604, 1202, 1497, 1522
BstMBI	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
BstMCI	CGRYCG	1 cut(s)	216
BstMWI	GCNNNNNNNGC	2 cut(s)	176, 1297
BstNI	CCWGG	4 cut(s)	714, 1268, 1311, 1533
BstNSI	RCATGY	1 cut(s)	845
BstSCI	CCNGG	8 cut(s)	58, 293, 294, 712, 1266, 1309, 1437, 1531
BstSFI	CTRYAG	2 cut(s)	261, 1382
BstSLI	GKGCMC	3 cut(s)	135, 1024, 1140
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	3 cut(s)	247, 275, 1396
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	2 cut(s)	484, 817
BstYI	RGATCY	2 cut(s)	484, 817
Bsu36I	CCTNAGG	1 cut(s)	87
BsuRI	GGCC	3 cut(s)	14, 133, 1022
BtgI	CCRYGG	1 cut(s)	148
BtgZI	GCGATG	1 cut(s)	1315
BtsCI	GGATG	6 cut(s)	121, 451, 559, 631, 1054, 1282
BtsIMutI	CAGTG	6 cut(s)	237, 346, 466, 605, 606, 1446
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	4 cut(s)	790, 843, 1389, 1400
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	2 cut(s)	220, 301
Cfr10I	RCCGGY	1 cut(s)	1221
Cfr13I	GGNCC	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
Cfr9I	CCCGGG	1 cut(s)	294
CseI	GACGC	2 cut(s)	24, 127
Csp6I	GTAC	9 cut(s)	9, 97, 109, 251, 384, 693, 1219, 1377, 1442
CspAI	ACCGGT	1 cut(s)	1221
CviAII	CATG	6 cut(s)	560, 705, 842, 1102, 1125, 1216
CviQI	GTAC	9 cut(s)	9, 97, 109, 251, 384, 693, 1219, 1377, 1442
DdeI	CTNAG	7 cut(s)	51, 87, 165, 302, 598, 608, 651
DpnI	GATC	8 cut(s)	5, 431, 452, 486, 540, 819, 1083, 1110
DpnII	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
DrdI	GACNNNNNNGTC	1 cut(s)	925
DseDI	GACNNNNNNGTC	1 cut(s)	925
EaeI	YGGCCR	1 cut(s)	12
Eam1104I	CTCTTC	1 cut(s)	943
EarI	CTCTTC	1 cut(s)	943
Eco130I	CCWWGG	1 cut(s)	889
Eco24I	GRGCYC	2 cut(s)	135, 1024
Eco31I	GGTCTC	2 cut(s)	68, 1522
Eco47I	GGWCC	3 cut(s)	291, 927, 1264
Eco57I	CTGAAG	2 cut(s)	89, 217
Eco81I	CCTNAGG	1 cut(s)	87
Eco88I	CYCGRG	1 cut(s)	294
EcoO109I	RGGNCCY	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
EcoRI	GAATTC	1 cut(s)	469
EcoRII	CCWGG	4 cut(s)	712, 1266, 1309, 1531
EcoT14I	CCWWGG	1 cut(s)	889
EcoT38I	GRGCYC	2 cut(s)	135, 1024
ErhI	CCWWGG	1 cut(s)	889
Esp3I	CGTCTC	1 cut(s)	1497
FaeI	CATG	6 cut(s)	563, 708, 845, 1105, 1128, 1219
FaqI	GGGAC	3 cut(s)	304, 1277, 1370
FatI	CATG	6 cut(s)	559, 704, 841, 1101, 1124, 1215
FbaI	TGATCA	2 cut(s)	3, 538
FblI	GTMKAC	1 cut(s)	594
Fnu4HI	GCNGC	5 cut(s)	261, 264, 1298, 1315, 1385
FokI	GGATG	6 cut(s)	108, 458, 566, 638, 1061, 1289
FriOI	GRGCYC	2 cut(s)	135, 1024
Fsp4HI	GCNGC	5 cut(s)	261, 264, 1298, 1315, 1385
FspBI	CTAG	2 cut(s)	42, 696
GlaI	GCGC	2 cut(s)	219, 300
GluI	GCNGC	5 cut(s)	261, 264, 1298, 1315, 1385
GsuI	CTGGAG	1 cut(s)	261
HaeII	RGCGCY	1 cut(s)	302
HaeIII	GGCC	3 cut(s)	14, 133, 1022
HapII	CCGG	4 cut(s)	59, 295, 1222, 1438
HgaI	GACGC	2 cut(s)	24, 127
HhaI	GCGC	2 cut(s)	220, 301
Hin1I	GRCGYC	1 cut(s)	35
Hin1II	CATG	6 cut(s)	563, 708, 845, 1105, 1128, 1219
Hin6I	GCGC	2 cut(s)	218, 299
HinP1I	GCGC	2 cut(s)	218, 299
HindIII	AAGCTT	4 cut(s)	101, 229, 638, 1396
HinfI	GANTC	4 cut(s)	754, 794, 1169, 1481
HpaII	CCGG	4 cut(s)	59, 295, 1222, 1438
HphI	GGTGA	7 cut(s)	599, 731, 1085, 1176, 1413, 1427, 1537
Hpy166II	GTNNAC	3 cut(s)	595, 1444, 1473
Hpy188I	TCNGA	3 cut(s)	1113, 1195, 1543
Hpy188III	TCNNGA	6 cut(s)	67, 476, 542, 560, 916, 1241
Hpy8I	GTNNAC	3 cut(s)	595, 1444, 1473
Hpy99I	CGWCG	1 cut(s)	40
HpyAV	CCTTC	3 cut(s)	135, 146, 544
HpyCH4III	ACNGT	7 cut(s)	20, 160, 725, 943, 1213, 1251, 1495
HpyCH4IV	ACGT	3 cut(s)	253, 847, 964
HpyCH4V	TGCA	9 cut(s)	28, 263, 314, 622, 704, 845, 1035, 1364, 1384
HpyF10VI	GCNNNNNNNGC	2 cut(s)	176, 1297
HpyF3I	CTNAG	7 cut(s)	51, 87, 165, 302, 598, 608, 651
HpySE526I	ACGT	3 cut(s)	253, 847, 964
Hsp92I	GRCGYC	1 cut(s)	35
Hsp92II	CATG	6 cut(s)	563, 708, 845, 1105, 1128, 1219
HspAI	GCGC	2 cut(s)	218, 299
KflI	GGGWCCC	1 cut(s)	291
Ksp22I	TGATCA	2 cut(s)	3, 538
Kzo9I	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
LmnI	GCTCC	4 cut(s)	205, 1098, 1322, 1396
Lsp1109I	GCAGC	3 cut(s)	247, 275, 1396
LweI	GCATC	4 cut(s)	15, 130, 436, 535
MaeI	CTAG	2 cut(s)	42, 696
MaeII	ACGT	3 cut(s)	253, 847, 964
MaeIII	GTNAC	7 cut(s)	160, 603, 680, 719, 860, 1010, 1182
MalI	GATC	8 cut(s)	5, 431, 452, 486, 540, 819, 1083, 1110
MboI	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
MboII	GAAGA	7 cut(s)	127, 216, 424, 445, 960, 1088, 1118
MfeI	CAATTG	1 cut(s)	617
MflI	RGATCY	2 cut(s)	484, 817
MhlI	GDGCHC	4 cut(s)	135, 652, 1024, 1140
MluCI	AATT	8 cut(s)	403, 421, 469, 511, 617, 827, 1189, 1415
MmeI	TCCRAC	2 cut(s)	610, 925
MnlI	CCTC	9 cut(s)	82, 236, 317, 320, 808, 1064, 1126, 1317, 1466
MroXI	GAANNNNTTC	1 cut(s)	1485
MseI	TTAA	1 cut(s)	186
MspA1I	CMGCKG	2 cut(s)	260, 1426
MspCI	CTTAAG	1 cut(s)	185
MspI	CCGG	4 cut(s)	59, 295, 1222, 1438
MspR9I	CCNGG	8 cut(s)	60, 295, 296, 714, 1268, 1311, 1439, 1533
MunI	CAATTG	1 cut(s)	617
MvaI	CCWGG	4 cut(s)	714, 1268, 1311, 1533
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	2 cut(s)	176, 1297
NciI	CCSGG	4 cut(s)	60, 295, 296, 1439
NdeII	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
NlaIII	CATG	6 cut(s)	563, 708, 845, 1105, 1128, 1219
NmeAIII	GCCGAG	1 cut(s)	1526
NmuCI	GTSAC	4 cut(s)	160, 603, 719, 1182
NspI	RCATGY	1 cut(s)	845
NspV	TTCGAA	1 cut(s)	401
PaeI	GCATGC	1 cut(s)	845
PagI	TCATGA	1 cut(s)	559
PcsI	WCGNNNNNNNCGW	4 cut(s)	156, 386, 744, 1178
PdmI	GAANNNNTTC	1 cut(s)	1485
PfeI	GAWTC	4 cut(s)	754, 794, 1169, 1481
Pfl23II	CGTACG	1 cut(s)	250
PinAI	ACCGGT	1 cut(s)	1221
PkrI	GCNGC	5 cut(s)	262, 265, 1299, 1316, 1386
Ppu21I	YACGTR	1 cut(s)	965
PpuMI	RGGWCCY	3 cut(s)	291, 927, 1264
PsiI	TTATAA	2 cut(s)	660, 852
Psp5II	RGGWCCY	3 cut(s)	291, 927, 1264
Psp6I	CCWGG	4 cut(s)	712, 1266, 1309, 1531
PspGI	CCWGG	4 cut(s)	712, 1266, 1309, 1531
PspLI	CGTACG	1 cut(s)	250
PspOMI	GGGCCC	2 cut(s)	131, 1020
PspPI	GGNCC	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
PspPPI	RGGWCCY	3 cut(s)	291, 927, 1264
PstI	CTGCAG	2 cut(s)	265, 1386
PsuI	RGATCY	2 cut(s)	484, 817
PvuII	CAGCTG	1 cut(s)	260
RsaI	GTAC	9 cut(s)	10, 98, 110, 252, 385, 694, 1220, 1378, 1443
RsaNI	GTAC	9 cut(s)	9, 97, 109, 251, 384, 693, 1219, 1377, 1442
SaqAI	TTAA	1 cut(s)	186
SatI	GCNGC	5 cut(s)	261, 264, 1298, 1315, 1385
Sau3AI	GATC	8 cut(s)	3, 429, 450, 484, 538, 817, 1081, 1108
Sau96I	GGNCC	7 cut(s)	131, 132, 291, 927, 1020, 1021, 1264
ScaI	AGTACT	1 cut(s)	385
ScrFI	CCNGG	8 cut(s)	60, 295, 296, 714, 1268, 1311, 1439, 1533
SduI	GDGCHC	4 cut(s)	135, 652, 1024, 1140
SfaNI	GCATC	4 cut(s)	15, 130, 436, 535
SfcI	CTRYAG	2 cut(s)	261, 1382
SfuI	TTCGAA	1 cut(s)	401
SinI	GGWCC	3 cut(s)	291, 927, 1264
SmaI	CCCGGG	1 cut(s)	296
SmlI	CTYRAG	3 cut(s)	65, 185, 474
SmoI	CTYRAG	3 cut(s)	65, 185, 474
SpeI	ACTAGT	1 cut(s)	695
SphI	GCATGC	1 cut(s)	845
Sse9I	AATT	8 cut(s)	403, 421, 469, 511, 617, 827, 1189, 1415
SsiI	CCGC	5 cut(s)	216, 585, 1297, 1314, 1424
SspMI	CTAG	2 cut(s)	42, 696
StyD4I	CCNGG	8 cut(s)	58, 293, 294, 712, 1266, 1309, 1437, 1531
StyI	CCWWGG	1 cut(s)	889
TaaI	ACNGT	7 cut(s)	20, 160, 725, 943, 1213, 1251, 1495
TaiI	ACGT	3 cut(s)	256, 850, 967
TaqI	TCGA	5 cut(s)	380, 389, 401, 917, 1084
TaqII	GACCGA	1 cut(s)	1226
TasI	AATT	8 cut(s)	403, 421, 469, 511, 617, 827, 1189, 1415
TatI	WGTACW	3 cut(s)	96, 383, 692
TauI	GCSGC	2 cut(s)	1300, 1317
TfiI	GAWTC	4 cut(s)	754, 794, 1169, 1481
Tru1I	TTAA	1 cut(s)	186
Tru9I	TTAA	1 cut(s)	186
TscAI	CASTG	6 cut(s)	244, 346, 466, 606, 612, 1453
TseFI	GTSAC	4 cut(s)	160, 603, 719, 1182
TseI	GCWGC	3 cut(s)	260, 263, 1384
Tsp45I	GTSAC	4 cut(s)	160, 603, 719, 1182
TspDTI	ATGAA	2 cut(s)	445, 783
TspGWI	ACGGA	1 cut(s)	1474
TspMI	CCCGGG	1 cut(s)	294
TspRI	CASTG	6 cut(s)	244, 346, 466, 606, 612, 1453
Vha464I	CTTAAG	1 cut(s)	185
VpaK11BI	GGWCC	3 cut(s)	291, 927, 1264
XapI	RAATTY	3 cut(s)	403, 469, 1189
XceI	RCATGY	1 cut(s)	845
XcmI	CCANNNNNNNNNTGG	1 cut(s)	873
XmaI	CCCGGG	1 cut(s)	294
XmiI	GTMKAC	1 cut(s)	594
XmnI	GAANNNNTTC	1 cut(s)	1485
XspI	CTAG	2 cut(s)	42, 696
ZrmI	AGTACT	1 cut(s)	385

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.