pycom04g12650

Domain of unknown function (DUF1929)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0017541
Physical Location & Seq
Forward (+)
15608116 .. 15609723
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g12650.1

Sequence Viewer

Length: 1608 bp
ATGCCTTCTGTTTCTCAGCAATGGCTGCTACTGCTTCTAATAACCACAGTACTAACAGTCTTCGCACGTGCCGACTTACCAGGCACGTGGGAGCTCCTCGTGGAGAACGCCGGCGTAGCCTCCATGCACACCGCCGTCACACGCTTCAATACCGTGGTGCTCCTCGACCGCACCAGCATCGGCCCTTCCCGCAAAATGCTCCGCAGGGGCCACTGCCGCAACGACCCCTACGACGCGGTTCTGAAGCGCGACTGCTACGCCCACTCCGTGCTCTTCGACCTCCAGACAAAGCAAATCCGCCCGCTCATGATCCAGACCGACACGTGGTGCTCATCCGGGCAGTTCCTCCCGGACGGCACGTTATTACAAACGGGTGGTGACCTGGACGGGGTGAAAAAGATCCGAAAATTCGTCCCGTGCGAGGCAACCGGGTCGTGCGACTGGGTGGAGCTTGCTGACGTGGAATTGGCCAACGGGAGGTGGTACGCCACGAATCAGATTCTGCCGGACGGGTCGGTGGCTATAGTCGGCGGGAGAGCGGCCAACACGGTGGAGTATTACCCGCCGAGAAAAGGCGCCGTTGCGCTCAATTTCCTTGCTGACGTGGAGGACAACCAAATGGACAATTTATACCCGTACGTGCATCTCCTCCCCAACGGCCACCTCTTCATCTTTGCTAACAACAAAGCGGTGTCGTACGATCACAACGTGGATAAAATCGTGAGGGAATATCCACCGTTGGATGGCGGCCCACGTAATTACCCGTCCGCCGGATCATCCGCAATGCTGGCGTTAGAAGGCGACTACTCAACGGCTGTGATTTTGGTTTGCGGCGGGGCCCCGTACGGCGCTTTCATCGCGAGGATGACCGACACCCCGGCGCACGGTAGCTGCGGGCGCATCGTAGCGACGTCGCCCGACCCGGTTTGGGAAATGGAGGACATGCCGTTCGGGAGGATTATGGGGGACATGGTCATGCTTCCAAGCGGCGACGTTTTGGTCATCAACGGAGCGCAAGCTGGGACCCAGGGCTTCGAGGCCGCATCCAACCCGTGCTTGTACCCGCTTCTTTACCGATCCGACCAGCCGGTCGGCTTGCGGTTCATGACTTTAAACCCAGGAACCGTGCCCCGATTGTACCACTCCACCGCGAATCTCTTACCCGACGGGCGGGTATTAATCGCGGGAAGCAACCCGCATTATTTCTACAGATTCGACATCGAGTTTCCCACGGAGTTACGAATCGAAGCGTTTTCGCCCGAGTATTTGAGTCCGGACCGGGCGAATCTCCGACCCATTATCGAGGGGATACCCGAAATGGTGCGCTACGGCGAGAGTTTTGACGTTTCGGTGTCGGTTCCGCTGTCCGTGGTGGGGGTTGTGGAGGTTAATTTGGGGAACGCGCCTTTTGCCACGCATTCGTTTTCGCAGGGTCAGCGGCTGGTCAAACTGGCCGTTACGCCGTCGGTGCCGGACGGGGAGGGGCGTTACAGGATTAGCTGTGTGGCGCCGCCGAATGGAATGGTTGCTCCGCCAGGTTATTACATGGCATTTGCGGTGAATCAGGGCGTGCCAAGTGTTGCGCGCTGGATACATCTGGTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

536

Amino Acids

58.44

Weight (kDa)

5.5

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 42 - 422 5.8e-168 Glyoxal oxidase N-terminus
GO-like_E_set PF09118 431 - 532 1.9e-27 Galactose oxidase-like, Early set domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017541)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53950
fragaria_vesca FvH4_6g12780
malus_domestica MD04G1138400.v1.1
prunus_persica Prupe.6G264000_v2.0.a1
pyrus_communis pycom04g12650
rosa_chinensis RchiOBHm_Chr3g0464771
rosa_laevigata RLG00000024673
rosa_multiflora Rmu_sc0007114.1_g000012
rosa_roxburghii Rroxscaffold_6G00415430
rosa_rugosa Rorug03G0074000
rosa_samantha Rh3BG136500
rosa_wichuraiana Rw3G011170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 998, 1088
AatII GACGTC 1 cut(s) 914
AccB1I GGYRCC 3 cut(s) 575, 1468, 1507
AccBSI CCGCTC 2 cut(s) 304, 539
AccII CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
AccIII TCCGGA 1 cut(s) 1273
AclWI GGATC 4 cut(s) 304, 394, 781, 1071
AcoI YGGCCR 4 cut(s) 468, 540, 658, 1452
AcsI RAATTY 1 cut(s) 407
AcuI CTGAAG 1 cut(s) 263
AcvI CACGTG 3 cut(s) 68, 87, 324
AcyI GRCGYC 3 cut(s) 576, 911, 1508
AdeI CACNNNGTG 3 cut(s) 268, 327, 709
AfaI GTAC 7 cut(s) 51, 485, 638, 698, 845, 1061, 1139
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 1 cut(s) 148
AjiI CACGTC 2 cut(s) 460, 604
AjnI CCWGG 5 cut(s) 79, 381, 1026, 1117, 1534
AloI GAACNNNNNNTCC 2 cut(s) 932, 964
AluBI AGCT 5 cut(s) 94, 451, 891, 1019, 1500
AluI AGCT 5 cut(s) 94, 451, 891, 1019, 1500
Alw21I GWGCWC 4 cut(s) 96, 162, 273, 332
AlwI GGATC 4 cut(s) 304, 394, 781, 1071
AlwNI CAGNNNCTG 2 cut(s) 502, 1441
Ama87I CYCGRG 1 cut(s) 1259
Aor13HI TCCGGA 1 cut(s) 1273
AoxI GGCC 9 cut(s) 181, 208, 468, 540, 658, 748, 837, 1038, 1452
ApaI GGGCCC 1 cut(s) 841
ApeKI GCWGC 2 cut(s) 25, 891
ApoI RAATTY 1 cut(s) 407
ArsI GACNNNNNNTTYG 2 cut(s) 932, 964
AseI ATTAAT 1 cut(s) 1178
AspS9I GGNCC 7 cut(s) 182, 208, 749, 837, 838, 1023, 1276
AsuC2I CCSGG 6 cut(s) 337, 350, 430, 878, 923, 1280
AsuHPI GGTGA 3 cut(s) 389, 403, 1570
AvaI CYCGRG 1 cut(s) 1259
AvaII GGWCC 2 cut(s) 1023, 1276
BaeGI GKGCMC 2 cut(s) 841, 1131
BalI TGGCCA 1 cut(s) 470
BanI GGYRCC 3 cut(s) 575, 1468, 1507
BanII GRGCYC 2 cut(s) 96, 841
BauI CACGAG 1 cut(s) 98
BbrPI CACGTG 3 cut(s) 68, 87, 324
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 4 cut(s) 96, 162, 273, 332
BbvI GCAGC 2 cut(s) 12, 878
BccI CCATC 1 cut(s) 737
BcgI CGANNNNNNTGC 4 cut(s) 160, 194, 414, 448
BciT130I CCWGG 5 cut(s) 81, 383, 1028, 1119, 1536
BciVI GTATCC 2 cut(s) 1302, 1584
BcnI CCSGG 6 cut(s) 337, 350, 430, 878, 923, 1280
BfmI CTRYAG 2 cut(s) 522, 1207
BfoI RGCGCY 3 cut(s) 579, 852, 1511
BfuI GTATCC 2 cut(s) 1302, 1584
BmcAI AGTACT 1 cut(s) 51
Bme18I GGWCC 2 cut(s) 1023, 1276
BmeT110I CYCGRG 1 cut(s) 1259
BmgBI CACGTC 2 cut(s) 460, 604
BmgT120I GGNCC 7 cut(s) 182, 208, 749, 837, 838, 1023, 1276
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 4 cut(s) 186, 652, 909, 1052
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 1 cut(s) 52
BpmI CTGGAG 1 cut(s) 266
BpuMI CCSGG 6 cut(s) 337, 350, 430, 878, 923, 1280
BsaAI YACGTR 5 cut(s) 68, 87, 324, 640, 755
BsaHI GRCGYC 3 cut(s) 576, 911, 1508
BsaJI CCNNGG 7 cut(s) 153, 876, 1026, 1027, 1117, 1230, 1368
BsaWI WCCGGW 1 cut(s) 1273
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bse118I RCCGGY 2 cut(s) 110, 1087
Bse1I ACTGG 2 cut(s) 446, 1455
Bse3DI GCAATG 2 cut(s) 26, 789
BseAI TCCGGA 1 cut(s) 1273
BseBI CCWGG 5 cut(s) 81, 383, 1028, 1119, 1536
BseDI CCNNGG 7 cut(s) 153, 876, 1026, 1027, 1117, 1230, 1368
BseGI GGATG 5 cut(s) 332, 748, 776, 870, 1043
BseMI GCAATG 2 cut(s) 26, 789
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 2 cut(s) 446, 1455
BsePI GCGCGC 1 cut(s) 1583
BseRI GAGGAG 3 cut(s) 86, 152, 638
BseSI GKGCMC 2 cut(s) 841, 1131
BseXI GCAGC 2 cut(s) 12, 878
BseYI CCCAGC 1 cut(s) 1019
Bsh1236I CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
Bsh1285I CGRYCG 2 cut(s) 169, 1092
BshFI GGCC 9 cut(s) 183, 210, 470, 542, 660, 750, 839, 1040, 1454
BshNI GGYRCC 3 cut(s) 575, 1468, 1507
BsiEI CGRYCG 2 cut(s) 169, 1092
BsiHKAI GWGCWC 4 cut(s) 96, 162, 273, 332
BsiHKCI CYCGRG 1 cut(s) 1259
BsiWI CGTACG 3 cut(s) 636, 696, 843
BslFI GGGAC 3 cut(s) 398, 980, 1036
BsmFI GGGAC 3 cut(s) 398, 980, 1036
BsmI GAATGC 1 cut(s) 1417
BsnI GGCC 9 cut(s) 183, 210, 470, 542, 660, 750, 839, 1040, 1454
BsoBI CYCGRG 1 cut(s) 1259
Bsp120I GGGCCC 1 cut(s) 837
Bsp1286I GDGCHC 6 cut(s) 96, 162, 273, 332, 841, 1131
Bsp13I TCCGGA 1 cut(s) 1273
Bsp143I GATC 5 cut(s) 309, 399, 700, 773, 1076
Bsp68I TCGCGA 1 cut(s) 860
BspANI GGCC 9 cut(s) 183, 210, 470, 542, 660, 750, 839, 1040, 1454
BspCNI CTCAG 1 cut(s) 28
BspEI TCCGGA 1 cut(s) 1273
BspFNI CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
BspHI TCATGA 2 cut(s) 306, 1104
BspPI GGATC 4 cut(s) 304, 394, 781, 1071
BspQI GCTCTTC 1 cut(s) 278
BspT107I GGYRCC 3 cut(s) 575, 1468, 1507
BsrBI CCGCTC 2 cut(s) 304, 539
BsrDI GCAATG 2 cut(s) 26, 789
BsrFI RCCGGY 2 cut(s) 110, 1087
BsrI ACTGG 2 cut(s) 446, 1455
BssAI RCCGGY 2 cut(s) 110, 1087
BssECI CCNNGG 7 cut(s) 153, 876, 1026, 1027, 1117, 1230, 1368
BssHII GCGCGC 1 cut(s) 1583
BssMI GATC 5 cut(s) 309, 399, 700, 773, 1076
BssNI GRCGYC 3 cut(s) 576, 911, 1508
BssSI CACGAG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 5 cut(s) 81, 383, 1028, 1119, 1536
Bst4CI ACNGT 7 cut(s) 49, 58, 154, 550, 738, 887, 1126
Bst6I CTCTTC 2 cut(s) 278, 671
BstACI GRCGYC 3 cut(s) 576, 911, 1508
BstAPI GCANNNNNTGC 1 cut(s) 25
BstBAI YACGTR 5 cut(s) 68, 87, 324, 640, 755
BstC8I GCNNGC 9 cut(s) 112, 302, 453, 789, 896, 1017, 1097, 1571, 1585
BstDEI CTNAG 1 cut(s) 15
BstDSI CCRYGG 3 cut(s) 153, 1230, 1368
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 5 cut(s) 332, 748, 776, 870, 1043
BstFNI CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
BstH2I RGCGCY 3 cut(s) 579, 852, 1511
BstKTI GATC 5 cut(s) 312, 402, 703, 776, 1079
BstMBI GATC 5 cut(s) 309, 399, 700, 773, 1076
BstMCI CGRYCG 2 cut(s) 169, 1092
BstNI CCWGG 5 cut(s) 81, 383, 1028, 1119, 1536
BstNSI RCATGY 1 cut(s) 946
BstPI GGTNACC 1 cut(s) 377
BstSFI CTRYAG 2 cut(s) 522, 1207
BstSLI GKGCMC 2 cut(s) 841, 1131
BstUI CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
BstV1I GCAGC 2 cut(s) 12, 878
BstV2I GAAGAC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 399
BstXI CCANNNNNNTGG 2 cut(s) 87, 550
BstYI RGATCY 1 cut(s) 399
BsuI GTATCC 2 cut(s) 1302, 1584
BsuRI GGCC 9 cut(s) 183, 210, 470, 542, 660, 750, 839, 1040, 1454
BtgI CCRYGG 3 cut(s) 153, 1230, 1368
BtgZI GCGATG 1 cut(s) 841
BtrI CACGTC 2 cut(s) 460, 604
BtsCI GGATG 5 cut(s) 332, 748, 776, 870, 1043
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 211
BtuMI TCGCGA 1 cut(s) 860
Cac8I GCNNGC 9 cut(s) 112, 302, 453, 789, 896, 1017, 1097, 1571, 1585
CaiI CAGNNNCTG 2 cut(s) 502, 1441
CciI TCATGA 2 cut(s) 306, 1104
Cfr10I RCCGGY 2 cut(s) 110, 1087
Cfr13I GGNCC 7 cut(s) 182, 208, 749, 837, 838, 1023, 1276
CpoI CGGWCCG 1 cut(s) 1276
CseI GACGC 1 cut(s) 242
Csp6I GTAC 7 cut(s) 50, 484, 637, 697, 844, 1060, 1138
CspI CGGWCCG 1 cut(s) 1276
CviAII CATG 7 cut(s) 124, 307, 943, 970, 976, 1105, 1546
CviQI GTAC 7 cut(s) 50, 484, 637, 697, 844, 1060, 1138
DdeI CTNAG 1 cut(s) 15
DinI GGCGCC 2 cut(s) 577, 1509
DpnI GATC 5 cut(s) 311, 401, 702, 775, 1078
DpnII GATC 5 cut(s) 309, 399, 700, 773, 1076
DraI TTTAAA 1 cut(s) 1113
DraIII CACNNNGTG 3 cut(s) 268, 327, 709
DrdI GACNNNNNNGTC 2 cut(s) 998, 1088
DseDI GACNNNNNNGTC 2 cut(s) 998, 1088
EaeI YGGCCR 4 cut(s) 468, 540, 658, 1452
Eam1104I CTCTTC 2 cut(s) 278, 671
EarI CTCTTC 2 cut(s) 278, 671
EciI GGCGGA 3 cut(s) 287, 757, 1521
Ecl136II GAGCTC 1 cut(s) 94
Eco24I GRGCYC 2 cut(s) 96, 841
Eco47I GGWCC 2 cut(s) 1023, 1276
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 263
Eco72I CACGTG 3 cut(s) 68, 87, 324
Eco88I CYCGRG 1 cut(s) 1259
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 3 cut(s) 837, 838, 1023
EcoO65I GGTNACC 1 cut(s) 377
EcoRII CCWGG 5 cut(s) 79, 381, 1026, 1117, 1534
EcoT38I GRGCYC 2 cut(s) 96, 841
EgeI GGCGCC 2 cut(s) 577, 1509
EheI GGCGCC 2 cut(s) 577, 1509
FaeI CATG 7 cut(s) 127, 310, 946, 973, 979, 1108, 1549
FaqI GGGAC 3 cut(s) 398, 980, 1036
FatI CATG 7 cut(s) 123, 306, 942, 969, 975, 1104, 1545
FokI GGATG 5 cut(s) 319, 755, 763, 877, 1030
FriOI GRGCYC 2 cut(s) 96, 841
GsaI CCCAGC 1 cut(s) 1023
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 3 cut(s) 579, 852, 1511
HaeIII GGCC 9 cut(s) 183, 210, 470, 542, 660, 750, 839, 1040, 1454
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 3 cut(s) 576, 911, 1508
Hin1II CATG 7 cut(s) 127, 310, 946, 973, 979, 1108, 1549
HinfI GANTC 8 cut(s) 493, 499, 1153, 1212, 1242, 1270, 1285, 1561
HphI GGTGA 3 cut(s) 389, 403, 1570
Hpy188I TCNGA 5 cut(s) 243, 404, 498, 1081, 1292
Hpy188III TCNNGA 9 cut(s) 283, 307, 313, 721, 859, 952, 1105, 1274, 1605
Hpy99I CGWCG 6 cut(s) 236, 913, 916, 995, 1169, 1468
HpyAV CCTTC 3 cut(s) 15, 195, 791
HpyCH4III ACNGT 7 cut(s) 49, 58, 154, 550, 738, 887, 1126
HpyCH4V TGCA 2 cut(s) 127, 643
HpyF3I CTNAG 1 cut(s) 15
Hsp92I GRCGYC 3 cut(s) 576, 911, 1508
Hsp92II CATG 7 cut(s) 127, 310, 946, 973, 979, 1108, 1549
KasI GGCGCC 2 cut(s) 575, 1507
KflI GGGWCCC 1 cut(s) 1023
Kpn2I TCCGGA 1 cut(s) 1273
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 5 cut(s) 309, 399, 700, 773, 1076
LguI GCTCTTC 1 cut(s) 278
LmnI GCTCC 7 cut(s) 91, 99, 165, 204, 448, 1010, 1534
Lsp1109I GCAGC 2 cut(s) 12, 878
LweI GCATC 4 cut(s) 186, 652, 909, 1052
MaeIII GTNAC 5 cut(s) 136, 377, 1236, 1456, 1487
MalI GATC 5 cut(s) 311, 401, 702, 775, 1078
MbiI CCGCTC 2 cut(s) 304, 539
MboI GATC 5 cut(s) 309, 399, 700, 773, 1076
MboII GAAGA 3 cut(s) 52, 265, 658
MflI RGATCY 1 cut(s) 399
MhlI GDGCHC 6 cut(s) 96, 162, 273, 332, 841, 1131
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 6 cut(s) 407, 464, 589, 625, 757, 1390
MluNI TGGCCA 1 cut(s) 470
Mly113I GGCGCC 2 cut(s) 576, 1508
MlyI GAGTC 1 cut(s) 1279
MmeI TCCRAC 4 cut(s) 720, 1071, 1104, 1315
Mox20I TGGCCA 1 cut(s) 470
MreI CGCCGGCG 1 cut(s) 110
MroI TCCGGA 1 cut(s) 1273
MroNI GCCGGC 1 cut(s) 110
MscI TGGCCA 1 cut(s) 470
MseI TTAA 3 cut(s) 1112, 1178, 1389
MslI CAYNNNNRTG 1 cut(s) 974
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 2 cut(s) 1363, 1438
Mva1269I GAATGC 1 cut(s) 1417
MvaI CCWGG 5 cut(s) 81, 383, 1028, 1119, 1536
MvnI CGCG 7 cut(s) 236, 249, 860, 1151, 1184, 1403, 1585
NaeI GCCGGC 1 cut(s) 112
NarI GGCGCC 2 cut(s) 576, 1508
NciI CCSGG 6 cut(s) 337, 350, 430, 878, 923, 1280
NdeII GATC 5 cut(s) 309, 399, 700, 773, 1076
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 7 cut(s) 127, 310, 946, 973, 979, 1108, 1549
NmeAIII GCCGAG 1 cut(s) 591
NmuCI GTSAC 2 cut(s) 136, 377
NruI TCGCGA 1 cut(s) 860
NspI RCATGY 1 cut(s) 946
PagI TCATGA 2 cut(s) 306, 1104
PasI CCCWGGG 1 cut(s) 1027
PauI GCGCGC 1 cut(s) 1583
PciSI GCTCTTC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 5 cut(s) 69, 228, 264, 417, 705
PctI GAATGC 1 cut(s) 1417
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 7 cut(s) 493, 499, 1153, 1212, 1242, 1285, 1561
Pfl23II CGTACG 3 cut(s) 636, 696, 843
PflFI GACNNNGTC 1 cut(s) 971
PfoI TCCNGGA 1 cut(s) 348
PleI GAGTC 1 cut(s) 1278
PluTI GGCGCC 2 cut(s) 579, 1511
PmaCI CACGTG 3 cut(s) 68, 87, 324
PmlI CACGTG 3 cut(s) 68, 87, 324
PpsI GAGTC 1 cut(s) 1278
Ppu21I YACGTR 5 cut(s) 68, 87, 324, 640, 755
PpuMI RGGWCCY 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 1178
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 1023
Psp6I CCWGG 5 cut(s) 79, 381, 1026, 1117, 1534
PspCI CACGTG 3 cut(s) 68, 87, 324
PspEI GGTNACC 1 cut(s) 377
PspFI CCCAGC 1 cut(s) 1019
PspGI CCWGG 5 cut(s) 79, 381, 1026, 1117, 1534
PspLI CGTACG 3 cut(s) 636, 696, 843
PspOMI GGGCCC 1 cut(s) 837
PspPI GGNCC 7 cut(s) 182, 208, 749, 837, 838, 1023, 1276
PspPPI RGGWCCY 1 cut(s) 1023
PstNI CAGNNNCTG 2 cut(s) 502, 1441
PsuI RGATCY 1 cut(s) 399
PsyI GACNNNGTC 1 cut(s) 971
PteI GCGCGC 1 cut(s) 1583
RruI TCGCGA 1 cut(s) 860
RsaI GTAC 7 cut(s) 51, 485, 638, 698, 845, 1061, 1139
RsaNI GTAC 7 cut(s) 50, 484, 637, 697, 844, 1060, 1138
RseI CAYNNNNRTG 1 cut(s) 974
Rsr2I CGGWCCG 1 cut(s) 1276
RsrII CGGWCCG 1 cut(s) 1276
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 278
SaqAI TTAA 3 cut(s) 1112, 1178, 1389
Sau3AI GATC 5 cut(s) 309, 399, 700, 773, 1076
Sau96I GGNCC 7 cut(s) 182, 208, 749, 837, 838, 1023, 1276
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 1 cut(s) 1279
SduI GDGCHC 6 cut(s) 96, 162, 273, 332, 841, 1131
SfaNI GCATC 4 cut(s) 186, 652, 909, 1052
SfcI CTRYAG 2 cut(s) 522, 1207
SfoI GGCGCC 2 cut(s) 577, 1509
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 2 cut(s) 1023, 1276
SmiMI CAYNNNNRTG 1 cut(s) 974
Sse9I AATT 6 cut(s) 407, 464, 589, 625, 757, 1390
SspDI GGCGCC 2 cut(s) 575, 1507
SstI GAGCTC 1 cut(s) 96
TaaI ACNGT 7 cut(s) 49, 58, 154, 550, 738, 887, 1126
TaqI TCGA 7 cut(s) 165, 276, 1035, 1215, 1221, 1245, 1302
TaqII GACCGA 2 cut(s) 332, 884
TasI AATT 6 cut(s) 407, 464, 589, 625, 757, 1390
TatI WGTACW 1 cut(s) 49
TauI GCSGC 8 cut(s) 219, 542, 750, 834, 990, 1043, 1441, 1513
TfiI GAWTC 7 cut(s) 493, 499, 1153, 1212, 1242, 1285, 1561
Tru1I TTAA 3 cut(s) 1112, 1178, 1389
Tru9I TTAA 3 cut(s) 1112, 1178, 1389
TscAI CASTG 1 cut(s) 218
TseFI GTSAC 2 cut(s) 136, 377
TseI GCWGC 2 cut(s) 25, 891
Tsp45I GTSAC 2 cut(s) 136, 377
TspDTI ATGAA 3 cut(s) 658, 844, 1093
TspGWI ACGGA 4 cut(s) 256, 1023, 1247, 1357
TspRI CASTG 1 cut(s) 218
Tth111I GACNNNGTC 1 cut(s) 971
VpaK11BI GGWCC 2 cut(s) 1023, 1276
VspI ATTAAT 1 cut(s) 1178
XapI RAATTY 1 cut(s) 407
XceI RCATGY 1 cut(s) 946
ZraI GACGTC 1 cut(s) 912
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.