pycom04g16110
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0018532
Physical Location & Seq
Reverse (-)
18840321 .. 18841478
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16110.2

Sequence Viewer

Length: 906 bp
ATGCAGGGAGGCCTCGAGTTCAAGACTGAAGTCGAGTTGCTCTCTCGCGTTCATCACAAAAATGTCGTTGGTCTTTTGGGATTCTGTTTCGAACAAGGAGAGCAGATGCTGGTTTACGAGTTTATGCCTAATGGAACACTTAGGGAAAGCTTGTCAGGGCGATCTGGTATTCATCTCGATTGGAAGAGGAGACTCCGAATCACTCTTGGCTCGGCAAGAGGACTGGCTTACCTACATGAGCTTGCAAATCCTCCTATAATTCACAGAGATGTGAAGTCCACCAATATTCTATTAGATGAACATTTAACAGCGAAGGTTGCAGATTTTGGCCTGTCGAAGCTCGTCAATGACAGCGGAAAAGGACATGTCTCGACTCAGGTCAAGGGAACAATGGGCTATCTGGATCCTGAGTACTACATGACTCAACAATTGACCGAGAAGAGCGATGTATACAGCTTTGGAGTCGTTATGCTTGAACTGATAACTGCTAGGCATCCAATCGAGAAGGGAAAATACATTGTCCGCGAGGTACAGTTGGTGATGGACAAGAATGACGAAGAGCATTACGGTTTGAGGGAGTTAATAGATCGAAACATTCGAAACTCAGGAACTCTTATTGGGTTTGGGAGGTTCTTGGAGCTGGCCATGCAATGTGTCGAAGAATCAGCTGCAGATCGTCCCATGATGAGTGAAGTTGTGAAGGCTATCGAAACCATTTTGCAGAACGATGGGATGAACACAAACTCAACATCAGCATCTTCATCAGCCACAGGGTTTGCAGCATCAAAAGGTGCTCCGAAACATCCCTACAACGATGGCTTGCCGAAAAAGGAATTCAATGACAGCACCGGCTCCCTTGATTATAGCGGTGGACACATTGTTTCAGCGAAAATCGAACCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Pkinase_Tyr

Protein Analysis

302

Amino Acids

33.42

Weight (kDa)

6.14

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018532)

Species Orthologous Gene IDs
pyrus_communis pycom04g16110
rosa_chinensis RchiOBHm_Chr5g0042201
rosa_multiflora Rmu_co8085702.1_g000001
rosa_samantha Rh5BG289200 Rh5BG289800 Rh5BG290000 Rh5CG320600 Rh5CG322000 Rh5CG322300 Rh5DG299200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 450
AccII CGCG 2 cut(s) 48, 525
AciI CCGC 3 cut(s) 354, 523, 867
AclWI GGATC 2 cut(s) 398, 411
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 2 cut(s) 413, 531
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 22, 476, 838
AluBI AGCT 6 cut(s) 150, 241, 340, 456, 640, 668
AluI AGCT 6 cut(s) 150, 241, 340, 456, 640, 668
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 2 cut(s) 184, 373
AlwI GGATC 2 cut(s) 398, 411
AlwNI CAGNNNCTG 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 14
AoxI GGCC 3 cut(s) 10, 328, 642
ApeKI GCWGC 2 cut(s) 668, 779
ApoI RAATTY 1 cut(s) 833
AsuHPI GGTGA 1 cut(s) 550
AsuII TTCGAA 2 cut(s) 90, 598
AvaI CYCGRG 1 cut(s) 14
BalI TGGCCA 1 cut(s) 644
BamHI GGATCC 1 cut(s) 403
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 2 cut(s) 655, 791
BccI CCATC 3 cut(s) 535, 722, 809
BcoDI GTCTC 2 cut(s) 184, 373
BfaI CTAG 1 cut(s) 489
BfmI CTRYAG 1 cut(s) 669
BisI GCNGC 2 cut(s) 669, 780
BlsI GCNGC 2 cut(s) 670, 781
BmcAI AGTACT 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 14
BmiI GGNNCC 2 cut(s) 405, 853
BmsI GCATC 4 cut(s) 96, 502, 764, 791
BoxI GACNNNNGTC 2 cut(s) 29, 377
BplI GAGNNNNNCTC 2 cut(s) 26, 58
Bpu14I TTCGAA 2 cut(s) 90, 598
Bse118I RCCGGY 1 cut(s) 848
Bse1I ACTGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 656
BseGI GGATG 3 cut(s) 493, 738, 802
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 3 cut(s) 389, 399, 618
BseNI ACTGG 1 cut(s) 228
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 2 cut(s) 655, 791
Bsh1236I CGCG 2 cut(s) 48, 525
BshFI GGCC 3 cut(s) 12, 330, 644
BsiHKAI GWGCWC 1 cut(s) 796
BsiHKCI CYCGRG 1 cut(s) 14
BsiSI CCGG 1 cut(s) 849
BslFI GGGAC 1 cut(s) 663
BsmAI GTCTC 2 cut(s) 184, 373
BsmFI GGGAC 1 cut(s) 663
BsnI GGCC 3 cut(s) 12, 330, 644
BsoBI CYCGRG 1 cut(s) 14
Bsp119I TTCGAA 2 cut(s) 90, 598
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 4 cut(s) 161, 403, 586, 673
BspACI CCGC 3 cut(s) 354, 523, 867
BspANI GGCC 3 cut(s) 12, 330, 644
BspCNI CTCAG 3 cut(s) 388, 400, 617
BspFNI CGCG 2 cut(s) 48, 525
BspLI GGNNCC 2 cut(s) 405, 853
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 2 cut(s) 398, 411
BspQI GCTCTTC 2 cut(s) 434, 552
BspT104I TTCGAA 2 cut(s) 90, 598
BsrDI GCAATG 1 cut(s) 656
BsrFI RCCGGY 1 cut(s) 848
BsrI ACTGG 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 848
BssMI GATC 4 cut(s) 161, 403, 586, 673
BssNAI GTATAC 1 cut(s) 451
Bst1107I GTATAC 1 cut(s) 451
Bst4CI ACNGT 2 cut(s) 534, 569
Bst6I CTCTTC 3 cut(s) 179, 434, 552
BstBI TTCGAA 2 cut(s) 90, 598
BstC8I GCNNGC 3 cut(s) 243, 642, 821
BstDEI CTNAG 4 cut(s) 140, 375, 408, 604
BstF5I GGATG 3 cut(s) 493, 738, 802
BstFNI CGCG 2 cut(s) 48, 525
BstKTI GATC 4 cut(s) 164, 406, 589, 676
BstMAI GTCTC 2 cut(s) 184, 373
BstMBI GATC 4 cut(s) 161, 403, 586, 673
BstMWI GCNNNNNNNGC 2 cut(s) 317, 646
BstNSI RCATGY 1 cut(s) 368
BstPAI GACNNNNGTC 2 cut(s) 29, 377
BstSFI CTRYAG 1 cut(s) 669
BstUI CGCG 2 cut(s) 48, 525
BstV1I GCAGC 2 cut(s) 655, 791
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BstZ17I GTATAC 1 cut(s) 451
BsuRI GGCC 3 cut(s) 12, 330, 644
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 3 cut(s) 493, 738, 802
Cac8I GCNNGC 3 cut(s) 243, 642, 821
CaiI CAGNNNCTG 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 848
Csp6I GTAC 2 cut(s) 412, 530
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 5 cut(s) 236, 365, 418, 646, 682
CviQI GTAC 2 cut(s) 412, 530
DdeI CTNAG 4 cut(s) 140, 375, 408, 604
DpnI GATC 4 cut(s) 163, 405, 588, 675
DpnII GATC 4 cut(s) 161, 403, 586, 673
EaeI YGGCCR 1 cut(s) 642
Eam1104I CTCTTC 3 cut(s) 179, 434, 552
EarI CTCTTC 3 cut(s) 179, 434, 552
Eco147I AGGCCT 1 cut(s) 12
Eco57I CTGAAG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 14
EcoRI GAATTC 1 cut(s) 833
FaeI CATG 5 cut(s) 239, 368, 421, 649, 685
FaqI GGGAC 1 cut(s) 663
FatI CATG 5 cut(s) 235, 364, 417, 645, 681
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 669, 780
FokI GGATG 3 cut(s) 480, 745, 789
Fsp4HI GCNGC 2 cut(s) 669, 780
FspBI CTAG 1 cut(s) 489
GluI GCNGC 2 cut(s) 669, 780
HaeIII GGCC 3 cut(s) 12, 330, 644
HapII CCGG 1 cut(s) 849
Hin1II CATG 5 cut(s) 239, 368, 421, 649, 685
HindIII AAGCTT 1 cut(s) 148
HinfI GANTC 7 cut(s) 81, 192, 198, 373, 421, 462, 662
HpaII CCGG 1 cut(s) 849
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 4 cut(s) 115, 279, 451, 872
Hpy188I TCNGA 2 cut(s) 197, 798
Hpy188III TCNNGA 7 cut(s) 22, 176, 370, 401, 407, 502, 606
Hpy8I GTNNAC 4 cut(s) 115, 279, 451, 872
HpyAV CCTTC 3 cut(s) 307, 499, 694
HpyCH4III ACNGT 2 cut(s) 534, 569
HpyCH4V TGCA 7 cut(s) 4, 245, 320, 649, 671, 721, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 646
HpyF3I CTNAG 4 cut(s) 140, 375, 408, 604
Hsp92II CATG 5 cut(s) 239, 368, 421, 649, 685
Kzo9I GATC 4 cut(s) 161, 403, 586, 673
LguI GCTCTTC 2 cut(s) 434, 552
LmnI GCTCC 3 cut(s) 637, 799, 857
Lsp1109I GCAGC 2 cut(s) 655, 791
LweI GCATC 4 cut(s) 96, 502, 764, 791
MaeI CTAG 1 cut(s) 489
MalI GATC 4 cut(s) 163, 405, 588, 675
MboI GATC 4 cut(s) 161, 403, 586, 673
MboII GAAGA 5 cut(s) 196, 451, 569, 671, 750
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 796
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 3 cut(s) 258, 428, 833
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 4 cut(s) 186, 367, 415, 471
MnlI CCTC 7 cut(s) 23, 180, 212, 261, 520, 567, 621
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 2 cut(s) 305, 581
MslI CAYNNNNRTG 2 cut(s) 60, 267
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 2 cut(s) 354, 668
MspI CCGG 1 cut(s) 849
MunI CAATTG 1 cut(s) 428
MvnI CGCG 2 cut(s) 48, 525
MwoI GCNNNNNNNGC 2 cut(s) 317, 646
NdeII GATC 4 cut(s) 161, 403, 586, 673
NlaIII CATG 5 cut(s) 239, 368, 421, 649, 685
NlaIV GGNNCC 2 cut(s) 405, 853
NmeAIII GCCGAG 1 cut(s) 191
NspI RCATGY 1 cut(s) 368
NspV TTCGAA 2 cut(s) 90, 598
PaeR7I CTCGAG 1 cut(s) 14
PceI AGGCCT 1 cut(s) 12
PciI ACATGT 1 cut(s) 364
PciSI GCTCTTC 2 cut(s) 434, 552
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PfeI GAWTC 3 cut(s) 81, 198, 662
PkrI GCNGC 2 cut(s) 670, 781
PleI GAGTC 4 cut(s) 186, 367, 415, 470
PpsI GAGTC 4 cut(s) 186, 367, 415, 470
PscI ACATGT 1 cut(s) 364
PshAI GACNNNNGTC 2 cut(s) 29, 377
PspN4I GGNNCC 2 cut(s) 405, 853
PspXI VCTCGAGB 1 cut(s) 14
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 403
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 413, 531
RsaNI GTAC 2 cut(s) 412, 530
RseI CAYNNNNRTG 2 cut(s) 60, 267
SapI GCTCTTC 2 cut(s) 434, 552
SaqAI TTAA 2 cut(s) 305, 581
SatI GCNGC 2 cut(s) 669, 780
Sau3AI GATC 4 cut(s) 161, 403, 586, 673
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 4 cut(s) 186, 367, 415, 471
SduI GDGCHC 1 cut(s) 796
SfaNI GCATC 4 cut(s) 96, 502, 764, 791
SfcI CTRYAG 1 cut(s) 669
Sfr274I CTCGAG 1 cut(s) 14
SfuI TTCGAA 2 cut(s) 90, 598
SlaI CTCGAG 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 60, 267
SmlI CTYRAG 1 cut(s) 14
SmoI CTYRAG 1 cut(s) 14
Sse9I AATT 3 cut(s) 258, 428, 833
SseBI AGGCCT 1 cut(s) 12
SsiI CCGC 3 cut(s) 354, 523, 867
SspI AATATT 1 cut(s) 286
SspMI CTAG 1 cut(s) 489
StuI AGGCCT 1 cut(s) 12
TaaI ACNGT 2 cut(s) 534, 569
TaqII GACCGA 1 cut(s) 449
TasI AATT 3 cut(s) 258, 428, 833
TatI WGTACW 1 cut(s) 411
TfiI GAWTC 3 cut(s) 81, 198, 662
Tru1I TTAA 2 cut(s) 305, 581
Tru9I TTAA 2 cut(s) 305, 581
TseI GCWGC 2 cut(s) 668, 779
TspDTI ATGAA 5 cut(s) 41, 161, 312, 749, 750
XapI RAATTY 1 cut(s) 833
XceI RCATGY 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 14
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 1 cut(s) 489
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.