pycom04g17310

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007733
Physical Location & Seq
Forward (+)
19741695 .. 19744724
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1671 bp
ATGAACTTCAACAGGCACTTTCGTGGCGAGGGTGACGAATCGAAAAGGAGACCTACTTTTGCCAATGTTGTTAGGGATGCGATGAGGGATGCCAGACTGAATGGCGAGTTGGAGCGCTTCTTTCGAAGAATAGTGCGAGATGAGTTGGAGGGCCATATTCTTCCCTTCTTCATGGCATCTCCAAGGCCCTCAAACGAAAGTGGGAGTACATCTGGAGGAAGCGGCTTGCAGTTGCGTTTTATCAACAAGCTGCCAACTACCATATTCACAGGCAGTAGAGTGGAAGCAGAGAACCGCGAGCCTCTTCAAATTGAACTGGTTGATGCCAGTACTGGTGCTATAGTCCATTCCGGTCCCCTATCTTCACTAAAGGTTGAACTTTTTGTGCTGAATGGTGAGTTTGGGTCAGATGATCAAGACGATTGGACCGAAAAAGAACTCAACACATACATTGTGCGAGAAAGGGAAGGCAAAAGGCCATTGGTGACGGGTGACAGACATGTTACACTGAAAGAGGGAGTTGGTTCTCTCGGTGATGTCATGTTTACCGACAACTCAAGCTGGATAAGATGCCGAAAGTTCAGACTAGCAGCTCGAGTTGTTGCCAAAGCTCGGAGTGAAGTTCGGATTAGGGAAGCTACAAGTGAACCGTTTGTGGTTAAAGATCACCGCGGAGAATTGTATAAGAAACACCACCCCCCACACCCAAAGGACGAAATATGGCGCCTGGAAAAGATATCAAAAGACGGTGCCCTTCATAAGAGACTCCACGAGAATGGAATCAGCACCGTGGAGGACTTCCTGCAGTCGTACATGAAAGATGCATCCTCGCTACGAAATGTTTTGCGTGGGATCTCGAACAAGATATGGGAAAGAATCGTACAGCATGCTATGGCCTGTAAGTTGGATGATCATAAGTTCTATGCCTACCACAGAGCTGAGCAGGACGAAAGTCTCCTGTTCAATTCCATCCACGGGCTTGAAGGGGCAATACTCAATGGCCAGTTCTGTTCTTTGGATGAACTCGACGCGCATCAGAAGATGCTGGTGGAAGCTTTGAAGCAGCAGGCTTATCGAAATGTAAGAGATTTGGAGCCCGTTGATGCTTCGACTATGTTTGGCCTTTCGAAGCCTTTGCCAGTTCTACAAGCTGATTCATTCACTTGTCCAAACCCTGACCTGCGGCAAAGTGAATTTCAGTTAACACATCAAGATGAACTCCCGAAACAACTAGGTTCCAACCATGCATCAACTTCAACCTCATGTCCCTACCAAGCAGAAGGTAGCAGCAATCAGCTAATGGTTTCTGTGGAACAACCTAGCCAGCCAATACAACTCCGAAGCAGCAGCTTTTCGATGGAGGATCTTAACTACATTATGTGCAACGGAGAAAGCAGTTGGTCGCCTCTCGGTGGTTTTGAGGCGCCAATTGTCCCAACTGGACTTTTAGGCACAGAAAACCCTTTCCAATTTCAAGCAGCAACTTGTTCCCCTACAAACCCAACATGGGGACAAGCAGGCGGCTTCTGCTTCGGTAGCGCAGGGACTAGACGGAAACCCAGGGCAAGCTGGCGCAAGCTTCGTGCCGCCATTAAGGGGTCGGTTTCGGTTAGGAGGGATGTGGCTGCTAAAAGAATGGCAAGGCGTCCTCCTGTGTATCTGAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0002229 GO:0002237 GO:0002239 GO:0002376 GO:0002682 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006725 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009696 GO:0009697 GO:0009787 GO:0009789 GO:0009814 GO:0009816 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010646 GO:0010647 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0031347 GO:0032101 GO:0032350 GO:0032787 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0046189 GO:0046394 GO:0046885 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070482 GO:0070887 GO:0071216 GO:0071219 GO:0071453 GO:0071456 GO:0071704 GO:0072330 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901421 GO:1901576 GO:1901615 GO:1901617 GO:1902584 GO:1903506 GO:1905957 GO:1905959 GO:2000070 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

557

Amino Acids

62.62

Weight (kDa)

8.5

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 76 - 223 2.4e-60 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 236 - 300 4.7e-27 Calmodulin binding protein central domain
Calmod_bind_C PF20452 306 - 367 4.8e-16 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1188
AccB1I GGYRCC 3 cut(s) 723, 749, 1423
AccII CGCG 3 cut(s) 297, 672, 1031
AciI CCGC 7 cut(s) 222, 295, 670, 672, 1183, 1521, 1587
AclWI GGATC 2 cut(s) 860, 1371
AcoI YGGCCR 1 cut(s) 1000
AcsI RAATTY 1 cut(s) 1193
AcyI GRCGYC 3 cut(s) 724, 1424, 1645
AfaI GTAC 4 cut(s) 208, 331, 812, 882
AfeI AGCGCT 1 cut(s) 116
AfiI CCNNNNNNNGG 6 cut(s) 612, 975, 1412, 1507, 1508, 1596
AflIII ACRYGT 1 cut(s) 499
AgsI TTSAA 9 cut(s) 10, 308, 314, 377, 964, 983, 1060, 1257, 1475
AjnI CCWGG 2 cut(s) 726, 1559
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
AleI CACNNNNGTG 1 cut(s) 21
Alw26I GTCTC 3 cut(s) 43, 757, 959
AlwI GGATC 2 cut(s) 860, 1371
Ama87I CYCGRG 1 cut(s) 594
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 6 cut(s) 151, 185, 476, 894, 1000, 1120
ApeKI GCWGC 8 cut(s) 250, 590, 1063, 1287, 1344, 1347, 1478, 1625
ApoI RAATTY 1 cut(s) 1193
AspLEI GCGC 6 cut(s) 117, 726, 1033, 1426, 1541, 1575
AspS9I GGNCC 4 cut(s) 151, 186, 353, 426
AsuHPI GGTGA 6 cut(s) 44, 407, 496, 503, 545, 659
AsuII TTCGAA 2 cut(s) 124, 1127
AvaI CYCGRG 1 cut(s) 594
AvaII GGWCC 2 cut(s) 353, 426
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 1 cut(s) 1002
BanI GGYRCC 3 cut(s) 723, 749, 1423
BanII GRGCYC 1 cut(s) 1098
BarI GAAGNNNNNNTAC 2 cut(s) 975, 1007
BauI CACGAG 1 cut(s) 770
BbvI GCAGC 8 cut(s) 237, 602, 1075, 1299, 1356, 1359, 1490, 1612
BccI CCATC 2 cut(s) 977, 1351
BcgI CGANNNNNNTGC 4 cut(s) 1055, 1089, 1117, 1151
BciT130I CCWGG 2 cut(s) 728, 1561
BclI TGATCA 2 cut(s) 412, 910
BcoDI GTCTC 3 cut(s) 43, 757, 959
BfaI CTAG 5 cut(s) 587, 1232, 1320, 1548, 1669
BfmI CTRYAG 2 cut(s) 339, 803
BfoI RGCGCY 3 cut(s) 118, 727, 1427
BfuAI ACCTGC 1 cut(s) 1188
BlpI GCTNAGC 1 cut(s) 939
BmcAI AGTACT 1 cut(s) 331
Bme1390I CCNGG 2 cut(s) 728, 1561
Bme18I GGWCC 2 cut(s) 353, 426
BmeT110I CYCGRG 1 cut(s) 594
BmgT120I GGNCC 4 cut(s) 151, 186, 353, 426
BmiI GGNNCC 6 cut(s) 355, 725, 751, 1095, 1237, 1425
BmrFI CCNGG 2 cut(s) 728, 1561
BoxI GACNNNNGTC 1 cut(s) 951
BpmI CTGGAG 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 2 cut(s) 124, 1127
BpuEI CTTGAG 1 cut(s) 541
BsaHI GRCGYC 3 cut(s) 724, 1424, 1645
BsaI GGTCTC 1 cut(s) 43
BsaJI CCNNGG 6 cut(s) 182, 670, 789, 973, 1559, 1560
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 6 cut(s) 612, 975, 1412, 1507, 1508, 1596
Bse1I ACTGG 6 cut(s) 321, 327, 337, 1003, 1139, 1444
BseBI CCWGG 2 cut(s) 728, 1561
BseDI CCNNGG 6 cut(s) 182, 670, 789, 973, 1559, 1560
BseGI GGATG 7 cut(s) 82, 94, 824, 913, 969, 1024, 1624
BseLI CCNNNNNNNGG 6 cut(s) 612, 975, 1412, 1507, 1508, 1596
BseMII CTCAG 1 cut(s) 930
BseNI ACTGG 6 cut(s) 321, 327, 337, 1003, 1139, 1444
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 8 cut(s) 237, 602, 1075, 1299, 1356, 1359, 1490, 1612
Bsh1236I CGCG 3 cut(s) 297, 672, 1031
BshFI GGCC 6 cut(s) 153, 187, 478, 896, 1002, 1122
BshNI GGYRCC 3 cut(s) 723, 749, 1423
BsiHKCI CYCGRG 1 cut(s) 594
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 5 cut(s) 339, 1251, 1418, 1524, 1558
BslI CCNNNNNNNGG 6 cut(s) 612, 975, 1412, 1507, 1508, 1596
BsmAI GTCTC 3 cut(s) 43, 757, 959
BsmFI GGGAC 5 cut(s) 339, 1251, 1418, 1524, 1558
BsnI GGCC 6 cut(s) 153, 187, 478, 896, 1002, 1122
Bso31I GGTCTC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 594
Bsp119I TTCGAA 2 cut(s) 124, 1127
Bsp1286I GDGCHC 2 cut(s) 754, 1098
Bsp143I GATC 5 cut(s) 412, 664, 852, 910, 1363
Bsp1720I GCTNAGC 1 cut(s) 939
BspACI CCGC 7 cut(s) 222, 295, 670, 672, 1183, 1521, 1587
BspANI GGCC 6 cut(s) 153, 187, 478, 896, 1002, 1122
BspCNI CTCAG 1 cut(s) 931
BspFNI CGCG 3 cut(s) 297, 672, 1031
BspLI GGNNCC 6 cut(s) 355, 725, 751, 1095, 1237, 1425
BspMAI CTGCAG 1 cut(s) 807
BspMI ACCTGC 1 cut(s) 1188
BspPI GGATC 2 cut(s) 860, 1371
BspT104I TTCGAA 2 cut(s) 124, 1127
BspT107I GGYRCC 3 cut(s) 723, 749, 1423
BspTNI GGTCTC 1 cut(s) 43
BsrI ACTGG 6 cut(s) 321, 327, 337, 1003, 1139, 1444
BssECI CCNNGG 6 cut(s) 182, 670, 789, 973, 1559, 1560
BssMI GATC 5 cut(s) 412, 664, 852, 910, 1363
BssNI GRCGYC 3 cut(s) 724, 1424, 1645
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 1 cut(s) 182
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 2 cut(s) 728, 1561
Bst4CI ACNGT 3 cut(s) 651, 749, 790
Bst6I CTCTTC 1 cut(s) 309
BstACI GRCGYC 3 cut(s) 724, 1424, 1645
BstBI TTCGAA 2 cut(s) 124, 1127
BstC8I GCNNGC 9 cut(s) 227, 299, 888, 1068, 1325, 1519, 1567, 1571, 1577
BstDEI CTNAG 1 cut(s) 939
BstDSI CCRYGG 3 cut(s) 670, 789, 973
BstF5I GGATG 7 cut(s) 82, 94, 824, 913, 969, 1024, 1624
BstFNI CGCG 3 cut(s) 297, 672, 1031
BstH2I RGCGCY 3 cut(s) 118, 727, 1427
BstHHI GCGC 6 cut(s) 117, 726, 1033, 1426, 1541, 1575
BstKTI GATC 5 cut(s) 415, 667, 855, 913, 1366
BstMAI GTCTC 3 cut(s) 43, 757, 959
BstMBI GATC 5 cut(s) 412, 664, 852, 910, 1363
BstMWI GCNNNNNNNGC 2 cut(s) 1527, 1536
BstNI CCWGG 2 cut(s) 728, 1561
BstNSI RCATGY 2 cut(s) 503, 890
BstPAI GACNNNNGTC 1 cut(s) 951
BstSCI CCNGG 2 cut(s) 726, 1559
BstSFI CTRYAG 2 cut(s) 339, 803
BstSLI GKGCMC 1 cut(s) 754
BstUI CGCG 3 cut(s) 297, 672, 1031
BstV1I GCAGC 8 cut(s) 237, 602, 1075, 1299, 1356, 1359, 1490, 1612
BstX2I RGATCY 2 cut(s) 852, 1363
BstXI CCANNNNNNTGG 1 cut(s) 776
BstYI RGATCY 2 cut(s) 852, 1363
BsuRI GGCC 6 cut(s) 153, 187, 478, 896, 1002, 1122
BtgI CCRYGG 3 cut(s) 670, 789, 973
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 7 cut(s) 82, 94, 824, 913, 969, 1024, 1624
BtsIMutI CAGTG 1 cut(s) 506
BveI ACCTGC 1 cut(s) 1188
Cac8I GCNNGC 9 cut(s) 227, 299, 888, 1068, 1325, 1519, 1567, 1571, 1577
CfoI GCGC 6 cut(s) 117, 726, 1033, 1426, 1541, 1575
Cfr13I GGNCC 4 cut(s) 151, 186, 353, 426
Cfr42I CCGCGG 1 cut(s) 673
CseI GACGC 2 cut(s) 1037, 1634
Csp6I GTAC 4 cut(s) 207, 330, 811, 881
CviAII CATG 8 cut(s) 172, 500, 541, 814, 887, 1244, 1263, 1506
CviQI GTAC 4 cut(s) 207, 330, 811, 881
DdeI CTNAG 1 cut(s) 939
DinI GGCGCC 2 cut(s) 725, 1425
DpnI GATC 5 cut(s) 414, 666, 854, 912, 1365
DpnII GATC 5 cut(s) 412, 664, 852, 910, 1363
EaeI YGGCCR 1 cut(s) 1000
Eam1104I CTCTTC 1 cut(s) 309
EarI CTCTTC 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 1 cut(s) 43
Eco32I GATATC 1 cut(s) 738
Eco47I GGWCC 2 cut(s) 353, 426
Eco47III AGCGCT 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 726, 1559
EcoRV GATATC 1 cut(s) 738
EcoT14I CCWWGG 1 cut(s) 182
EcoT22I ATGCAT 2 cut(s) 826, 1249
EcoT38I GRGCYC 1 cut(s) 1098
EgeI GGCGCC 2 cut(s) 725, 1425
EheI GGCGCC 2 cut(s) 725, 1425
ErhI CCWWGG 1 cut(s) 182
FaeI CATG 8 cut(s) 175, 503, 544, 817, 890, 1247, 1266, 1509
FaqI GGGAC 5 cut(s) 339, 1251, 1418, 1524, 1558
FatI CATG 8 cut(s) 171, 499, 540, 813, 886, 1243, 1262, 1505
FbaI TGATCA 2 cut(s) 412, 910
FokI GGATG 7 cut(s) 89, 101, 811, 920, 956, 1031, 1631
FriOI GRGCYC 1 cut(s) 1098
FspBI CTAG 5 cut(s) 587, 1232, 1320, 1548, 1669
GlaI GCGC 6 cut(s) 116, 725, 1032, 1425, 1540, 1574
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 3 cut(s) 118, 727, 1427
HaeIII GGCC 6 cut(s) 153, 187, 478, 896, 1002, 1122
HapII CCGG 1 cut(s) 351
HgaI GACGC 2 cut(s) 1037, 1634
HhaI GCGC 6 cut(s) 117, 726, 1033, 1426, 1541, 1575
Hin1I GRCGYC 3 cut(s) 724, 1424, 1645
Hin1II CATG 8 cut(s) 175, 503, 544, 817, 890, 1247, 1266, 1509
Hin6I GCGC 6 cut(s) 115, 724, 1031, 1424, 1539, 1573
HinP1I GCGC 6 cut(s) 115, 724, 1031, 1424, 1539, 1573
HincII GTYRAC 1 cut(s) 1203
HindII GTYRAC 1 cut(s) 1203
HindIII AAGCTT 2 cut(s) 1053, 1577
HinfI GANTC 5 cut(s) 38, 765, 780, 876, 1154
HpaI GTTAAC 1 cut(s) 1203
HpaII CCGG 1 cut(s) 351
HphI GGTGA 6 cut(s) 44, 407, 496, 503, 545, 659
Hpy166II GTNNAC 3 cut(s) 546, 647, 1203
Hpy188I TCNGA 7 cut(s) 409, 584, 615, 627, 1038, 1340, 1662
Hpy188III TCNNGA 5 cut(s) 213, 416, 856, 1211, 1222
Hpy8I GTNNAC 3 cut(s) 546, 647, 1203
Hpy99I CGWCG 1 cut(s) 1031
HpyAV CCTTC 5 cut(s) 175, 461, 764, 977, 1274
HpyCH4III ACNGT 3 cut(s) 651, 749, 790
HpyCH4V TGCA 5 cut(s) 229, 805, 824, 1247, 1383
HpyF10VI GCNNNNNNNGC 2 cut(s) 1527, 1536
HpyF3I CTNAG 1 cut(s) 939
Hsp92I GRCGYC 3 cut(s) 724, 1424, 1645
Hsp92II CATG 8 cut(s) 175, 503, 544, 817, 890, 1247, 1266, 1509
HspAI GCGC 6 cut(s) 115, 724, 1031, 1424, 1539, 1573
KasI GGCGCC 2 cut(s) 723, 1423
Ksp22I TGATCA 2 cut(s) 412, 910
KspAI GTTAAC 1 cut(s) 1203
KspI CCGCGG 1 cut(s) 673
Kzo9I GATC 5 cut(s) 412, 664, 852, 910, 1363
LmnI GCTCC 2 cut(s) 112, 1093
Lsp1109I GCAGC 8 cut(s) 237, 602, 1075, 1299, 1356, 1359, 1490, 1612
MaeI CTAG 5 cut(s) 587, 1232, 1320, 1548, 1669
MaeIII GTNAC 4 cut(s) 32, 484, 491, 502
MalI GATC 5 cut(s) 414, 666, 854, 912, 1365
MboI GATC 5 cut(s) 412, 664, 852, 910, 1363
MboII GAAGA 6 cut(s) 138, 152, 160, 296, 354, 1051
MfeI CAATTG 1 cut(s) 1428
MflI RGATCY 2 cut(s) 852, 1363
MhlI GDGCHC 2 cut(s) 754, 1098
MlsI TGGCCA 1 cut(s) 1002
MluCI AATT 6 cut(s) 309, 677, 964, 1193, 1428, 1469
MluNI TGGCCA 1 cut(s) 1002
Mly113I GGCGCC 2 cut(s) 724, 1424
MlyI GAGTC 1 cut(s) 759
MmeI TCCRAC 4 cut(s) 90, 126, 885, 1263
Mox20I TGGCCA 1 cut(s) 1002
Mph1103I ATGCAT 2 cut(s) 826, 1249
MscI TGGCCA 1 cut(s) 1002
MseI TTAA 4 cut(s) 660, 1202, 1368, 1593
MslI CAYNNNNRTG 3 cut(s) 21, 774, 1212
Msp20I TGGCCA 1 cut(s) 1002
MspA1I CMGCKG 1 cut(s) 672
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 2 cut(s) 728, 1561
MunI CAATTG 1 cut(s) 1428
MvaI CCWGG 2 cut(s) 728, 1561
MvnI CGCG 3 cut(s) 297, 672, 1031
MwoI GCNNNNNNNGC 2 cut(s) 1527, 1536
NarI GGCGCC 2 cut(s) 724, 1424
NdeII GATC 5 cut(s) 412, 664, 852, 910, 1363
NlaIII CATG 8 cut(s) 175, 503, 544, 817, 890, 1247, 1266, 1509
NlaIV GGNNCC 6 cut(s) 355, 725, 751, 1095, 1237, 1425
NmuCI GTSAC 3 cut(s) 32, 484, 491
NsiI ATGCAT 2 cut(s) 826, 1249
NspI RCATGY 2 cut(s) 503, 890
NspV TTCGAA 2 cut(s) 124, 1127
OliI CACNNNNGTG 1 cut(s) 21
PaeI GCATGC 1 cut(s) 890
PaeR7I CTCGAG 1 cut(s) 594
PasI CCCWGGG 1 cut(s) 1560
PciI ACATGT 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PfeI GAWTC 4 cut(s) 38, 780, 876, 1154
PleI GAGTC 1 cut(s) 759
PluTI GGCGCC 2 cut(s) 727, 1427
PpsI GAGTC 1 cut(s) 759
PscI ACATGT 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 951
Psp6I CCWGG 2 cut(s) 726, 1559
PspGI CCWGG 2 cut(s) 726, 1559
PspN4I GGNNCC 6 cut(s) 355, 725, 751, 1095, 1237, 1425
PspPI GGNCC 4 cut(s) 151, 186, 353, 426
PspXI VCTCGAGB 1 cut(s) 594
PstI CTGCAG 1 cut(s) 807
PsuI RGATCY 2 cut(s) 852, 1363
RsaI GTAC 4 cut(s) 208, 331, 812, 882
RsaNI GTAC 4 cut(s) 207, 330, 811, 881
RseI CAYNNNNRTG 3 cut(s) 21, 774, 1212
SacII CCGCGG 1 cut(s) 673
SaqAI TTAA 4 cut(s) 660, 1202, 1368, 1593
Sau3AI GATC 5 cut(s) 412, 664, 852, 910, 1363
Sau96I GGNCC 4 cut(s) 151, 186, 353, 426
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 2 cut(s) 728, 1561
SduI GDGCHC 2 cut(s) 754, 1098
SfcI CTRYAG 2 cut(s) 339, 803
SfoI GGCGCC 2 cut(s) 725, 1425
Sfr274I CTCGAG 1 cut(s) 594
Sfr303I CCGCGG 1 cut(s) 673
SfuI TTCGAA 2 cut(s) 124, 1127
SgrBI CCGCGG 1 cut(s) 673
SinI GGWCC 2 cut(s) 353, 426
SlaI CTCGAG 1 cut(s) 594
SmiMI CAYNNNNRTG 3 cut(s) 21, 774, 1212
SmlI CTYRAG 2 cut(s) 556, 594
SmoI CTYRAG 2 cut(s) 556, 594
SphI GCATGC 1 cut(s) 890
Sse9I AATT 6 cut(s) 309, 677, 964, 1193, 1428, 1469
SsiI CCGC 7 cut(s) 222, 295, 670, 672, 1183, 1521, 1587
SspDI GGCGCC 2 cut(s) 723, 1423
SspMI CTAG 5 cut(s) 587, 1232, 1320, 1548, 1669
StyD4I CCNGG 2 cut(s) 726, 1559
StyI CCWWGG 1 cut(s) 182
TaaI ACNGT 3 cut(s) 651, 749, 790
TaqI TCGA 9 cut(s) 41, 124, 595, 857, 1026, 1075, 1109, 1127, 1355
TaqII GACCGA 1 cut(s) 443
TasI AATT 6 cut(s) 309, 677, 964, 1193, 1428, 1469
TatI WGTACW 2 cut(s) 206, 329
TauI GCSGC 4 cut(s) 225, 1186, 1524, 1589
TfiI GAWTC 4 cut(s) 38, 780, 876, 1154
Tru1I TTAA 4 cut(s) 660, 1202, 1368, 1593
Tru9I TTAA 4 cut(s) 660, 1202, 1368, 1593
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 3 cut(s) 32, 484, 491
TseI GCWGC 8 cut(s) 250, 590, 1063, 1287, 1344, 1347, 1478, 1625
Tsp45I GTSAC 3 cut(s) 32, 484, 491
TspDTI ATGAA 7 cut(s) 17, 160, 746, 830, 1035, 1146, 1230
TspGWI ACGGA 2 cut(s) 1401, 1567
TspRI CASTG 1 cut(s) 513
VpaK11BI GGWCC 2 cut(s) 353, 426
XapI RAATTY 1 cut(s) 1193
XceI RCATGY 2 cut(s) 503, 890
XhoI CTCGAG 1 cut(s) 594
XspI CTAG 5 cut(s) 587, 1232, 1320, 1548, 1669
ZrmI AGTACT 1 cut(s) 331
Zsp2I ATGCAT 2 cut(s) 826, 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.