pycom04g18320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0018057
Physical Location & Seq
Reverse (-)
20390284 .. 20390832
549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGGCATCTAGCAGTAGTAGCAGGAGCAGTAAAGGCGAAGCCGTTGCTGCAGAAACTAGTACTCCTTCTCCATTCAAGATCAAACTCGAAGTAGATCACTTGCACACTGGCGTCCTCAAGATTGATCCTTCTAAGGAATTCGAAGAGCACGTTGTAGGGAACATGACCGCGGCTAATCAAAAGTTACTACACGAGTGGAAACCGGTTGAGTTTACATTCAAAGATGTCGATACAGATCAAACTTACCCGATGACTTTGCAGAAGATGGAGTCCTATAAATTTCTTAGTGATCAATCAAAGGAACAAGAAGCTATTGATTTGAGAAAGGCGAAGGATAACTTTTTCTACACCATTAGGCCTTCTAGTCGTGCGATGGACGAAAGGCAACTCATGACTTTTGGAGATCAAGAGATTGGACTGCCATGGACAGCGCCGTCAGTTGACAGCAACAAGCCCGCACTTGAAGTTTCGGTTTTGTATGTTCCGAAGCTCGACCTTCCAAACCATCAGGAGACGAAATGGTTGATATTTCAGGGACGTAGAGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.75

Weight (kDa)

5.76

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018057)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g24123
prunus_persica Prupe.6G327900_v2.0.a1
pyrus_communis pycom04g18320
rosa_laevigata RLG00000023301
rosa_multiflora Rmu_sc0002280.1_g000003
rosa_roxburghii Rroxscaffold_6G00398630
rosa_rugosa Rorug03G0204800
rosa_samantha Rh3AG252900 Rh3BG289500 Rh3CG286900 Rh3DG282400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AccII CGCG 1 cut(s) 170
AciI CCGC 3 cut(s) 168, 170, 456
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 2 cut(s) 137, 278
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 61
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 3 cut(s) 76, 220, 464
AhlI ACTAGT 1 cut(s) 56
AluBI AGCT 2 cut(s) 311, 490
AluI AGCT 2 cut(s) 311, 490
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 1 cut(s) 119
AoxI GGCC 1 cut(s) 356
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 137, 278
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 1 cut(s) 433
AsuII TTCGAA 1 cut(s) 141
BauI CACGAG 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 34
BccI CCATC 3 cut(s) 259, 367, 513
BceAI ACGGC 2 cut(s) 26, 418
BcgI CGANNNNNNTGC 4 cut(s) 26, 60, 238, 272
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 506
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 3 cut(s) 9, 57, 363
BfmI CTRYAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 434
BisI GCNGC 2 cut(s) 48, 171
BlsI GCNGC 2 cut(s) 49, 172
BmcAI AGTACT 1 cut(s) 61
BmsI GCATC 1 cut(s) 14
Bpu14I TTCGAA 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 234
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 168, 422
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 46, 52, 76, 82
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 234
BseDI CCNNGG 2 cut(s) 168, 422
BseJI GATNNNNATC 1 cut(s) 234
BseNI ACTGG 1 cut(s) 112
BseXI GCAGC 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 1 cut(s) 358
BshTI ACCGGT 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 506
BsmBI CGTCTC 1 cut(s) 506
BsnI GGCC 1 cut(s) 358
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 6 cut(s) 78, 94, 124, 235, 289, 403
Bsp19I CCATGG 1 cut(s) 422
BspACI CCGC 3 cut(s) 168, 170, 456
BspANI GGCC 1 cut(s) 358
BspFNI CGCG 1 cut(s) 170
BspHI TCATGA 1 cut(s) 390
BspMAI CTGCAG 1 cut(s) 52
BspPI GGATC 1 cut(s) 119
BspQI GCTCTTC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 168, 422
BssMI GATC 6 cut(s) 78, 94, 124, 235, 289, 403
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 422
Bst2BI CACGAG 1 cut(s) 191
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 2 cut(s) 132, 284
BstDSI CCRYGG 2 cut(s) 168, 422
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 434
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 6 cut(s) 81, 97, 127, 238, 292, 406
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 6 cut(s) 78, 94, 124, 235, 289, 403
BstMWI GCNNNNNNNGC 3 cut(s) 18, 33, 47
BstSFI CTRYAG 1 cut(s) 48
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 1 cut(s) 34
BsuRI GGCC 1 cut(s) 358
BtgI CCRYGG 2 cut(s) 168, 422
BtgZI GCGATG 1 cut(s) 386
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 456
CciI TCATGA 1 cut(s) 390
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 171
CseI GACGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 60
CspAI ACCGGT 1 cut(s) 202
CviAII CATG 3 cut(s) 163, 391, 423
CviJI RGCY 6 cut(s) 41, 173, 311, 358, 454, 490
CviKI_1 RGCY 6 cut(s) 41, 173, 311, 358, 454, 490
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 132, 284
DpnI GATC 6 cut(s) 80, 96, 126, 237, 291, 405
DpnII GATC 6 cut(s) 78, 94, 124, 235, 289, 403
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 138
EarI CTCTTC 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 422
Eco147I AGGCCT 1 cut(s) 358
EcoRI GAATTC 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 422
ErhI CCWWGG 1 cut(s) 422
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 3 cut(s) 166, 394, 426
FaiI YATR 6 cut(s) 164, 276, 392, 424, 480, 547
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FatI CATG 3 cut(s) 162, 390, 422
FauI CCCGC 1 cut(s) 463
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 48, 171
Fsp4HI GCNGC 2 cut(s) 48, 171
FspBI CTAG 3 cut(s) 9, 57, 363
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 48, 171
HaeII RGCGCY 1 cut(s) 434
HaeIII GGCC 1 cut(s) 358
HapII CCGG 1 cut(s) 203
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 3 cut(s) 166, 394, 426
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 203
Hpy166II GTNNAC 2 cut(s) 213, 442
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 5 cut(s) 76, 118, 391, 407, 509
Hpy8I GTNNAC 2 cut(s) 213, 442
HpyAV CCTTC 5 cut(s) 75, 138, 325, 369, 506
HpyCH4IV ACGT 2 cut(s) 150, 538
HpyCH4V TGCA 3 cut(s) 50, 103, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 18, 33, 47
HpyF3I CTNAG 2 cut(s) 132, 284
HpySE526I ACGT 2 cut(s) 150, 538
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 3 cut(s) 166, 394, 426
HspAI GCGC 1 cut(s) 431
Ksp22I TGATCA 1 cut(s) 289
KspI CCGCGG 1 cut(s) 171
Kzo9I GATC 6 cut(s) 78, 94, 124, 235, 289, 403
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 24
LpnPI CCDG 5 cut(s) 7, 93, 216, 494, 518
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 1 cut(s) 14
MaeI CTAG 3 cut(s) 9, 57, 363
MaeII ACGT 2 cut(s) 150, 538
MaeIII GTNAC 1 cut(s) 183
MalI GATC 6 cut(s) 80, 96, 126, 237, 291, 405
MboI GATC 6 cut(s) 78, 94, 124, 235, 289, 403
MboII GAAGA 2 cut(s) 155, 274
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 2 cut(s) 137, 278
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 3 cut(s) 18, 33, 47
NcoI CCATGG 1 cut(s) 422
NdeII GATC 6 cut(s) 78, 94, 124, 235, 289, 403
NlaIII CATG 3 cut(s) 166, 394, 426
NspV TTCGAA 1 cut(s) 141
PagI TCATGA 1 cut(s) 390
PceI AGGCCT 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 2 cut(s) 49, 172
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PstI CTGCAG 1 cut(s) 52
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SacII CCGCGG 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 138
SatI GCNGC 2 cut(s) 48, 171
Sau3AI GATC 6 cut(s) 78, 94, 124, 235, 289, 403
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 153, 313, 492, 498, 541
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 48
Sfr303I CCGCGG 1 cut(s) 171
SfuI TTCGAA 1 cut(s) 141
SgrBI CCGCGG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SpeI ACTAGT 1 cut(s) 56
Sse9I AATT 2 cut(s) 137, 278
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 3 cut(s) 168, 170, 456
SspMI CTAG 3 cut(s) 9, 57, 363
StuI AGGCCT 1 cut(s) 358
StyI CCWWGG 1 cut(s) 422
TaiI ACGT 2 cut(s) 153, 541
TaqI TCGA 4 cut(s) 87, 141, 228, 492
TasI AATT 2 cut(s) 137, 278
TatI WGTACW 1 cut(s) 59
TauI GCSGC 1 cut(s) 173
TscAI CASTG 1 cut(s) 112
TseI GCWGC 1 cut(s) 47
TspRI CASTG 1 cut(s) 112
XapI RAATTY 2 cut(s) 137, 278
XspI CTAG 3 cut(s) 9, 57, 363
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.