pycom04g19470

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0017895
Physical Location & Seq
Forward (+)
21189584 .. 21190879
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1296 bp
ATGGCTCCCGACCAAGACGACGCCGTTCTCCCCGAAGGCGACCTCTCCTCCTCCTCTCAGGACGAGGAAGAAGACGACGACGTCGTCGATGAGGACGAGGAAGAAGATGACGTCGACGTCGACGACGACGACGACGTCATCAACTCCGCCTCCCCGTCCACGTCATCTGTCGGCGCCACGGATTCTCTCCCCGTCGCCATCGCCACCGCAACTCCAACCGTCACCGTCGCCCTTCCCGCTCGGGCCCCGCCTACCGTGCCTCTGACCCTCGCTTCTGCTCCAACCACCGTAATCACAAACGACGCCGTGTTGTCGTCCGATCCCAAACGGCACCCCGCGTTGTCGTCCCAGCCGGAGGAGAAAAAACCGGCTGTGCTCGACGATTCTCGGAGATTATTTCAGAGGCTGTGGACCGACGAGGACGAGATCGAGCTTTTACAGGGCTTCCTCGACTACACGACGCAGCGAGGCAGCAGAGGCTCGCACCAGGGCCCAAACGACACCGCTTTGTTCTACGACCAGATCAAGTGCAAGCTCCAGCTTGACTTCAACAAGAACCAGCTCGTCGAGAAGCTCCGGCGGCTGAAGAAGAAGTACCGAAACGTAGTCACCAAAATCGAGAGCGGCAAAGAGGTCAGCTTCAAGAGCCCTCACGATCAGGCCACCTTCGAGATCTCCCGCAAGATCTGGAGCAATATCGGCCGAATTGGCGCCGCCGACGAGAACGCCCTCGACGATGAAGATCCGAGCCCTAACCCTAACAATCCTAATTTCAACAGTTACGAGATCAAGGCCGAGGAGGCTGCCGGTTTCGCCGGCGATAAGAAGTCCACGCCGAGATCACGCAAACGCTCGCGAATACAGCCAAGGGCCGACGAGAAACCCCCTCCTCCGCCGCTACGTGGCGGTTTCGTTGAGCCAACACCGGTAATCAAAGATAATAACAACGGCATTAACAGTAACAATGGTGGTGCTGGTACTAATGGCAGCAATTGTAATGTTCACGGGTTGATTGAGGAGACGGTGAAGAGTTGTCTGTCTCCATTGTTCAAGGAGTTACTGAGTAATGCAATGGGTGTCGGGCCATCCAGAGGGTTCGGAGGGTTGGCATTCAATCCGATTCCATTGAGCTTCGGCGGGCCTTTGATGAACTTGAATTTCGGGGGCGGGGAAGTGGCGGATGACAAGTGGAGGAAGCAGCAGATTTTGGAGTTGGAGGTGTACTCGAAGCGATTGGACTTGGTTCAGAATCAGATAAAAGTTGCATTGGATGAGTTGCGGTCCAATGGCGGGTGA

Protein Analysis

432

Amino Acids

46.89

Weight (kDa)

4.65

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 133 - 231 1.1e-31 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04930 AT3G04930 AT5G28040 AT5G28040
fragaria_vesca FvH4_6g03660
malus_domestica MD04G1219700.v1.1 MD12G1236500.v1.1
prunus_persica Prupe.6G338800_v2.0.a1 Prupe.6G338800_v2.0.a1
pyrus_communis pycom04g19470
rosa_samantha Rh6BG252100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 80, 83, 116, 134
AatII GACGTC 4 cut(s) 84, 114, 120, 138
AccB1I GGYRCC 3 cut(s) 173, 330, 710
AccBSI CCGCTC 2 cut(s) 239, 624
AccI GTMKAC 2 cut(s) 114, 120
AccII CGCG 2 cut(s) 338, 856
AclWI GGATC 2 cut(s) 314, 737
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 1 cut(s) 1156
AcuI CTGAAG 1 cut(s) 605
AcyI GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
AfaI GTAC 3 cut(s) 596, 979, 1223
AfiI CCNNNNNNNGG 4 cut(s) 355, 902, 1091, 1290
AgeI ACCGGT 1 cut(s) 925
AgsI TTSAA 6 cut(s) 550, 643, 775, 1051, 1114, 1156
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 1 cut(s) 486
AluBI AGCT 7 cut(s) 433, 535, 541, 562, 574, 639, 1131
AluI AGCT 7 cut(s) 433, 535, 541, 562, 574, 639, 1131
Alw21I GWGCWC 1 cut(s) 378
Alw26I GTCTC 2 cut(s) 1013, 1044
AlwI GGATC 2 cut(s) 314, 737
AlwNI CAGNNNCTG 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 8 cut(s) 243, 490, 660, 700, 792, 870, 1082, 1139
ApaI GGGCCC 2 cut(s) 247, 494
ApeKI GCWGC 5 cut(s) 463, 471, 803, 987, 1198
ApoI RAATTY 1 cut(s) 1156
AsiGI ACCGGT 1 cut(s) 925
AspLEI GCGC 2 cut(s) 176, 713
AspS9I GGNCC 9 cut(s) 243, 244, 411, 490, 491, 870, 1082, 1139, 1281
AsuHPI GGTGA 3 cut(s) 214, 601, 1036
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 2 cut(s) 411, 1281
BaeGI GKGCMC 2 cut(s) 247, 494
BanI GGYRCC 3 cut(s) 173, 330, 710
BanII GRGCYC 4 cut(s) 247, 494, 650, 752
BarI GAAGNNNNNNTAC 2 cut(s) 578, 610
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 378
BbvI GCAGC 5 cut(s) 475, 483, 790, 999, 1210
BccI CCATC 2 cut(s) 206, 1093
BceAI ACGGC 4 cut(s) 8, 290, 344, 964
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 1 cut(s) 488
BcoDI GTCTC 2 cut(s) 1013, 1044
BfoI RGCGCY 2 cut(s) 177, 714
BglI GCCNNNNNGGC 2 cut(s) 708, 800
BglII AGATCT 2 cut(s) 672, 684
BisI GCNGC 9 cut(s) 464, 472, 581, 625, 714, 804, 896, 988, 1199
BlsI GCNGC 9 cut(s) 465, 473, 582, 626, 715, 805, 897, 989, 1200
Bme1390I CCNGG 1 cut(s) 488
Bme18I GGWCC 2 cut(s) 411, 1281
BmeT110I CYCGRG 1 cut(s) 240
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 9 cut(s) 243, 244, 411, 490, 491, 870, 1082, 1139, 1281
BmiI GGNNCC 7 cut(s) 6, 175, 245, 246, 332, 492, 712
BmrFI CCNGG 1 cut(s) 488
BpiI GAAGAC 1 cut(s) 78
BplI GAGNNNNNCTC 2 cut(s) 1208, 1240
BpmI CTGGAG 2 cut(s) 521, 709
BsaAI YACGTR 1 cut(s) 902
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
BsaJI CCNNGG 4 cut(s) 177, 487, 795, 866
BsaWI WCCGGW 1 cut(s) 925
BsaXI ACNNNNNCTCC 6 cut(s) 32, 62, 134, 164, 196, 226
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 902, 1091, 1290
Bse118I RCCGGY 4 cut(s) 367, 806, 815, 925
Bse3DI GCAATG 1 cut(s) 1077
Bse8I GATNNNNATC 1 cut(s) 741
BseBI CCWGG 1 cut(s) 488
BseDI CCNNGG 4 cut(s) 177, 487, 795, 866
BseGI GGATG 3 cut(s) 1085, 1186, 1276
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 4 cut(s) 355, 902, 1091, 1290
BseMI GCAATG 1 cut(s) 1077
BseMII CTCAG 2 cut(s) 71, 1052
BseRI GAGGAG 7 cut(s) 37, 40, 43, 371, 812, 879, 1031
BseSI GKGCMC 2 cut(s) 247, 494
BseX3I CGGCCG 1 cut(s) 700
BseXI GCAGC 5 cut(s) 475, 483, 790, 999, 1210
BseYI CCCAGC 1 cut(s) 348
Bsh1236I CGCG 2 cut(s) 338, 856
Bsh1285I CGRYCG 1 cut(s) 703
BshFI GGCC 8 cut(s) 245, 492, 662, 702, 794, 872, 1084, 1141
BshNI GGYRCC 3 cut(s) 173, 330, 710
BshTI ACCGGT 1 cut(s) 925
BsiEI CGRYCG 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 378
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 6 cut(s) 353, 368, 577, 807, 816, 926
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 4 cut(s) 355, 902, 1091, 1290
BsmAI GTCTC 2 cut(s) 1013, 1044
BsmBI CGTCTC 1 cut(s) 1013
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 8 cut(s) 245, 492, 662, 702, 794, 872, 1084, 1141
BsoBI CYCGRG 1 cut(s) 240
Bsp120I GGGCCC 2 cut(s) 243, 490
Bsp1286I GDGCHC 5 cut(s) 247, 378, 494, 650, 752
Bsp143I GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
Bsp68I TCGCGA 1 cut(s) 856
BspANI GGCC 8 cut(s) 245, 492, 662, 702, 794, 872, 1084, 1141
BspCNI CTCAG 2 cut(s) 70, 1053
BspFNI CGCG 2 cut(s) 338, 856
BspLI GGNNCC 7 cut(s) 6, 175, 245, 246, 332, 492, 712
BspPI GGATC 2 cut(s) 314, 737
BspT107I GGYRCC 3 cut(s) 173, 330, 710
BsrBI CCGCTC 2 cut(s) 239, 624
BsrDI GCAATG 1 cut(s) 1077
BsrFI RCCGGY 4 cut(s) 367, 806, 815, 925
BssAI RCCGGY 4 cut(s) 367, 806, 815, 925
BssECI CCNNGG 4 cut(s) 177, 487, 795, 866
BssMI GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
BssNI GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
BssT1I CCWWGG 1 cut(s) 866
Bst2UI CCWGG 1 cut(s) 488
Bst4CI ACNGT 7 cut(s) 220, 226, 256, 289, 779, 959, 1024
Bst6I CTCTTC 1 cut(s) 1022
BstACI GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
BstBAI YACGTR 1 cut(s) 902
BstC8I GCNNGC 5 cut(s) 482, 533, 817, 854, 1139
BstDEI CTNAG 2 cut(s) 57, 1061
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 3 cut(s) 1085, 1186, 1276
BstFNI CGCG 2 cut(s) 338, 856
BstH2I RGCGCY 2 cut(s) 177, 714
BstHHI GCGC 2 cut(s) 176, 713
BstKTI GATC 9 cut(s) 322, 429, 525, 658, 675, 687, 745, 789, 842
BstMAI GTCTC 2 cut(s) 1013, 1044
BstMBI GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
BstMCI CGRYCG 1 cut(s) 703
BstNI CCWGG 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 486
BstSLI GKGCMC 2 cut(s) 247, 494
BstUI CGCG 2 cut(s) 338, 856
BstV1I GCAGC 5 cut(s) 475, 483, 790, 999, 1210
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 3 cut(s) 672, 684, 742
BstYI RGATCY 3 cut(s) 672, 684, 742
BstZI CGGCCG 1 cut(s) 700
BsuRI GGCC 8 cut(s) 245, 492, 662, 702, 794, 872, 1084, 1141
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 184
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 3 cut(s) 1085, 1186, 1276
BtuMI TCGCGA 1 cut(s) 856
Cac8I GCNNGC 5 cut(s) 482, 533, 817, 854, 1139
CaiI CAGNNNCTG 1 cut(s) 406
CfoI GCGC 2 cut(s) 176, 713
Cfr10I RCCGGY 4 cut(s) 367, 806, 815, 925
Cfr13I GGNCC 9 cut(s) 243, 244, 411, 490, 491, 870, 1082, 1139, 1281
CseI GACGC 3 cut(s) 29, 311, 469
Csp6I GTAC 3 cut(s) 595, 978, 1222
CspAI ACCGGT 1 cut(s) 925
CviQI GTAC 3 cut(s) 595, 978, 1222
DdeI CTNAG 2 cut(s) 57, 1061
DinI GGCGCC 2 cut(s) 175, 712
DpnI GATC 9 cut(s) 321, 428, 524, 657, 674, 686, 744, 788, 841
DpnII GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
DrdI GACNNNNNNGTC 4 cut(s) 80, 83, 116, 134
DseDI GACNNNNNNGTC 4 cut(s) 80, 83, 116, 134
EaeI YGGCCR 1 cut(s) 700
EagI CGGCCG 1 cut(s) 700
Eam1104I CTCTTC 1 cut(s) 1022
EarI CTCTTC 1 cut(s) 1022
EciI GGCGGA 3 cut(s) 136, 882, 1193
EclXI CGGCCG 1 cut(s) 700
Eco130I CCWWGG 1 cut(s) 866
Eco24I GRGCYC 4 cut(s) 247, 494, 650, 752
Eco47I GGWCC 2 cut(s) 411, 1281
Eco52I CGGCCG 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 240
EcoO109I RGGNCCY 2 cut(s) 244, 490
EcoRII CCWGG 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 866
EcoT38I GRGCYC 4 cut(s) 247, 494, 650, 752
EgeI GGCGCC 2 cut(s) 175, 712
EheI GGCGCC 2 cut(s) 175, 712
ErhI CCWWGG 1 cut(s) 866
Esp3I CGTCTC 1 cut(s) 1013
FaqI GGGAC 1 cut(s) 331
FauI CCCGC 7 cut(s) 244, 255, 343, 686, 1130, 1160, 1283
FblI GTMKAC 2 cut(s) 114, 120
Fnu4HI GCNGC 9 cut(s) 464, 472, 581, 625, 714, 804, 896, 988, 1199
FokI GGATG 3 cut(s) 1072, 1193, 1283
FriOI GRGCYC 4 cut(s) 247, 494, 650, 752
Fsp4HI GCNGC 9 cut(s) 464, 472, 581, 625, 714, 804, 896, 988, 1199
GlaI GCGC 2 cut(s) 175, 712
GluI GCNGC 9 cut(s) 464, 472, 581, 625, 714, 804, 896, 988, 1199
GsaI CCCAGC 1 cut(s) 352
GsuI CTGGAG 2 cut(s) 521, 709
HaeII RGCGCY 2 cut(s) 177, 714
HaeIII GGCC 8 cut(s) 245, 492, 662, 702, 794, 872, 1084, 1141
HapII CCGG 6 cut(s) 353, 368, 577, 807, 816, 926
HgaI GACGC 3 cut(s) 29, 311, 469
HhaI GCGC 2 cut(s) 176, 713
Hin1I GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
Hin6I GCGC 2 cut(s) 174, 711
HinP1I GCGC 2 cut(s) 174, 711
HincII GTYRAC 2 cut(s) 115, 121
HindII GTYRAC 2 cut(s) 115, 121
HinfI GANTC 4 cut(s) 182, 383, 1120, 1249
HpaII CCGG 6 cut(s) 353, 368, 577, 807, 816, 926
HphI GGTGA 3 cut(s) 214, 601, 1036
Hpy166II GTNNAC 7 cut(s) 115, 121, 159, 411, 831, 1003, 1222
Hpy188I TCNGA 9 cut(s) 264, 319, 390, 402, 747, 1100, 1119, 1248, 1254
Hpy8I GTNNAC 7 cut(s) 115, 121, 159, 411, 831, 1003, 1222
HpyAV CCTTC 3 cut(s) 29, 242, 676
HpyCH4III ACNGT 7 cut(s) 220, 226, 256, 289, 779, 959, 1024
HpyCH4IV ACGT 7 cut(s) 81, 111, 117, 135, 161, 603, 901
HpyCH4V TGCA 3 cut(s) 531, 1070, 1265
HpyF3I CTNAG 2 cut(s) 57, 1061
HpySE526I ACGT 7 cut(s) 81, 111, 117, 135, 161, 603, 901
Hsp92I GRCGYC 8 cut(s) 21, 81, 111, 117, 135, 174, 303, 711
HspAI GCGC 2 cut(s) 174, 711
KasI GGCGCC 2 cut(s) 173, 710
KroI GCCGGC 1 cut(s) 815
KroNI GCCGGC 1 cut(s) 817
Kzo9I GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
LmnI GCTCC 5 cut(s) 10, 283, 540, 579, 690
Lsp1109I GCAGC 5 cut(s) 475, 483, 790, 999, 1210
MaeII ACGT 7 cut(s) 81, 111, 117, 135, 161, 603, 901
MaeIII GTNAC 5 cut(s) 220, 607, 779, 959, 1056
MalI GATC 9 cut(s) 321, 428, 524, 657, 674, 686, 744, 788, 841
MbiI CCGCTC 2 cut(s) 239, 624
MboI GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
MboII GAAGA 8 cut(s) 80, 83, 113, 116, 598, 601, 752, 1039
MfeI CAATTG 1 cut(s) 991
MflI RGATCY 3 cut(s) 672, 684, 742
MhlI GDGCHC 5 cut(s) 247, 378, 494, 650, 752
MluCI AATT 4 cut(s) 705, 769, 991, 1156
Mly113I GGCGCC 2 cut(s) 174, 711
MmeI TCCRAC 3 cut(s) 239, 305, 1194
MreI CGCCGGCG 1 cut(s) 815
MroNI GCCGGC 1 cut(s) 815
MseI TTAA 1 cut(s) 954
MspI CCGG 6 cut(s) 353, 368, 577, 807, 816, 926
MspR9I CCNGG 1 cut(s) 488
MunI CAATTG 1 cut(s) 991
Mva1269I GAATGC 1 cut(s) 1109
MvaI CCWGG 1 cut(s) 488
MvnI CGCG 2 cut(s) 338, 856
NaeI GCCGGC 1 cut(s) 817
NarI GGCGCC 2 cut(s) 174, 711
NdeII GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
NgoMIV GCCGGC 1 cut(s) 815
NlaIV GGNNCC 7 cut(s) 6, 175, 245, 246, 332, 492, 712
NmeAIII GCCGAG 2 cut(s) 820, 861
NmuCI GTSAC 2 cut(s) 220, 607
NruI TCGCGA 1 cut(s) 856
PctI GAATGC 1 cut(s) 1109
PdiI GCCGGC 1 cut(s) 817
PfeI GAWTC 4 cut(s) 182, 383, 1120, 1249
PflFI GACNNNGTC 3 cut(s) 80, 83, 134
PinAI ACCGGT 1 cut(s) 925
PkrI GCNGC 9 cut(s) 465, 473, 582, 626, 715, 805, 897, 989, 1200
PluTI GGCGCC 2 cut(s) 177, 714
Ppu21I YACGTR 1 cut(s) 902
Psp6I CCWGG 1 cut(s) 486
PspFI CCCAGC 1 cut(s) 348
PspGI CCWGG 1 cut(s) 486
PspN4I GGNNCC 7 cut(s) 6, 175, 245, 246, 332, 492, 712
PspOMI GGGCCC 2 cut(s) 243, 490
PspPI GGNCC 9 cut(s) 243, 244, 411, 490, 491, 870, 1082, 1139, 1281
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 3 cut(s) 672, 684, 742
PsyI GACNNNGTC 3 cut(s) 80, 83, 134
RruI TCGCGA 1 cut(s) 856
RsaI GTAC 3 cut(s) 596, 979, 1223
RsaNI GTAC 3 cut(s) 595, 978, 1222
SalI GTCGAC 2 cut(s) 113, 119
SaqAI TTAA 1 cut(s) 954
SatI GCNGC 9 cut(s) 464, 472, 581, 625, 714, 804, 896, 988, 1199
Sau3AI GATC 9 cut(s) 319, 426, 522, 655, 672, 684, 742, 786, 839
Sau96I GGNCC 9 cut(s) 243, 244, 411, 490, 491, 870, 1082, 1139, 1281
ScrFI CCNGG 1 cut(s) 488
SduI GDGCHC 5 cut(s) 247, 378, 494, 650, 752
SfoI GGCGCC 2 cut(s) 175, 712
SgrAI CRCCGGYG 1 cut(s) 815
SgrDI CGTCGACG 2 cut(s) 113, 119
SinI GGWCC 2 cut(s) 411, 1281
Sse9I AATT 4 cut(s) 705, 769, 991, 1156
SspDI GGCGCC 2 cut(s) 173, 710
StyD4I CCNGG 1 cut(s) 486
StyI CCWWGG 1 cut(s) 866
TaaI ACNGT 7 cut(s) 220, 226, 256, 289, 779, 959, 1024
TaiI ACGT 7 cut(s) 84, 114, 120, 138, 164, 606, 904
TaqII GACCGA 1 cut(s) 428
TasI AATT 4 cut(s) 705, 769, 991, 1156
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 4 cut(s) 583, 627, 716, 898
TfiI GAWTC 4 cut(s) 182, 383, 1120, 1249
Tru1I TTAA 1 cut(s) 954
Tru9I TTAA 1 cut(s) 954
TseFI GTSAC 2 cut(s) 220, 607
TseI GCWGC 5 cut(s) 463, 471, 803, 987, 1198
Tsp45I GTSAC 2 cut(s) 220, 607
TspDTI ATGAA 2 cut(s) 753, 1163
TspGWI ACGGA 1 cut(s) 194
Tth111I GACNNNGTC 3 cut(s) 80, 83, 134
VpaK11BI GGWCC 2 cut(s) 411, 1281
XapI RAATTY 1 cut(s) 1156
XmiI GTMKAC 2 cut(s) 114, 120
ZraI GACGTC 4 cut(s) 82, 112, 118, 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.