pycom05g02380

One-helix protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015932
Physical Location & Seq
Reverse (-)
2812104 .. 2814340
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02380.1

Sequence Viewer

Length: 1029 bp
ATGAGCTTCATTCCTTGGTTGGCTATTAAATGCAAGCTTTCTATCATGGATAGAATTTTGACTTTTGCTCTGTCAGTTCCTGTCACTTGCATACTCTGCTCTATTGCTACGGAGTCCCATTGCCACCTCTTCTTCGAATGTCCTTTTACCAATGGTATTTGGTCTTTGGTGCTATTCAAATGTGGTGTCCCTTGGCTAAGGCTTCGTTGGCCTCTTTTTGTCATGTGGGCTACTAGCCATTGCAAGGGAAAATCTTTATCATCAATAGTTTTGAAGTTAAGTCTTGCTGCTACAGTAGTGACTTTAGAATTAGAATTCGAAGGAAGCCAGGCATCTCCTTTTTCACAAACTTGGCCGCACCTTATCCACAAAAAGCACTTCCAACTCCATCTGCTTCAATGGATAACACAAACAAATCCCAACCCCAAATTCCTCAGAAAGCAAAAGAACACGAAAATGTCAGTGGCATCTTCCTTCCCCTGCATCAAAACCCCACCTGCATCACCATCTTCCTGCTCACATTCTTCTTCTTGTTCCTCAAACCATTCTTTTATGCTCTCTTCTTCCTCTTCAACAAAGCCTTACTATGTCAAGTTCAGCATAAGGAGCTCCCAAACTGAAGGCCCTATCAGAAGACCTGGTGCTCCTTCTCTCCGGGAACCCTCCCGCCCGTCTTCCCCTCTCACACCAACGCCTCCTTCTCCTCCGTCTCCTTCTCCGCCGCCTCAGCAGCCCTCGAAGCCGGAACAGGTTTCCGGGCTGACCATGGTGGAGGACATGAATGCGGTGACCTTGGAGTTCCAGAGGCAGAAGGCTAAGGAGATTCAGGAGTACTTCAAGCAGAAGAAGCTTGAGGAAGCTAACCAGGGTCCTTTTTTTGGCTTTGTTGGCAAGAATGAGATTAGCAATGGCAGATGGGCAATGTTCGGTTTTGCTGTTGGAATGCTAACAGAGTATGCAACTGGCTCGGACTTTGTTGATCAAGTAAAGATACTTCTCTCCAATTTCGGAATTCTAGATCTGGAATGA

Protein Analysis

343

Amino Acids

38.18

Weight (kDa)

9.29

Isoelectric Point (pI)

61.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 1 - 54 5.2e-10 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015932)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34000
fragaria_vesca FvH4_2g13070
malus_domestica MD05G1032800.v1.1 MD10G1033800.v1.1
prunus_persica Prupe.8G039900_v2.0.a1
pyrus_communis pycom05g02380 pycom10g02230
rosa_chinensis RchiOBHm_Chr6g0274331
rosa_laevigata RLG00000013552
rosa_roxburghii Rroxscaffold_7G00194350
rosa_rugosa Rorug06G0080900
rosa_samantha Rh6AG196100 Rh6BG200300 Rh6CG201200
rosa_wichuraiana Rw6G017140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 505
AciI CCGC 5 cut(s) 356, 667, 719, 722, 785
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 4 cut(s) 54, 314, 428, 1011
AcuI CTGAAG 1 cut(s) 639
AfaI GTAC 1 cut(s) 833
AfiI CCNNNNNNNGG 2 cut(s) 244, 878
AgsI TTSAA 5 cut(s) 178, 274, 398, 573, 838
AjnI CCWGG 3 cut(s) 327, 637, 864
AjuI GAANNNNNNNTTGG 4 cut(s) 413, 445, 682, 714
AluBI AGCT 5 cut(s) 6, 37, 609, 850, 860
AluI AGCT 5 cut(s) 6, 37, 609, 850, 860
Alw21I GWGCWC 2 cut(s) 611, 646
Alw26I GTCTC 1 cut(s) 714
AlwNI CAGNNNCTG 1 cut(s) 80
AoxI GGCC 3 cut(s) 209, 353, 622
ApeKI GCWGC 2 cut(s) 287, 730
ApoI RAATTY 4 cut(s) 54, 314, 428, 1011
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
AspS9I GGNCC 2 cut(s) 623, 869
AsuC2I CCSGG 2 cut(s) 656, 757
AsuHPI GGTGA 2 cut(s) 495, 799
AsuII TTCGAA 2 cut(s) 135, 318
AvaII GGWCC 1 cut(s) 869
BanII GRGCYC 1 cut(s) 611
BarI GAAGNNNNNNTAC 2 cut(s) 978, 1010
BbsI GAAGAC 2 cut(s) 640, 666
Bbv12I GWGCWC 2 cut(s) 611, 646
BbvCI CCTCAGC 1 cut(s) 726
BbvI GCAGC 2 cut(s) 274, 742
BccI CCATC 3 cut(s) 396, 514, 909
BcgI CGANNNNNNTGC 2 cut(s) 948, 982
BciT130I CCWGG 3 cut(s) 329, 639, 866
BclI TGATCA 1 cut(s) 979
BcnI CCSGG 2 cut(s) 656, 757
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 2 cut(s) 234, 1016
BfmI CTRYAG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 505
BglII AGATCT 1 cut(s) 1018
BisI GCNGC 4 cut(s) 288, 356, 722, 731
BlsI GCNGC 4 cut(s) 289, 357, 723, 732
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 5 cut(s) 329, 639, 656, 757, 866
Bme18I GGWCC 1 cut(s) 869
BmgT120I GGNCC 2 cut(s) 623, 869
BmiI GGNNCC 2 cut(s) 660, 870
BmrFI CCNGG 5 cut(s) 329, 639, 656, 757, 866
BmsI GCATC 4 cut(s) 341, 476, 492, 509
BpiI GAAGAC 2 cut(s) 640, 666
Bpu10I CCTNAGC 3 cut(s) 197, 726, 816
Bpu14I TTCGAA 2 cut(s) 135, 318
BpuEI CTTGAG 1 cut(s) 872
BpuMI CCSGG 2 cut(s) 656, 757
BsaJI CCNNGG 5 cut(s) 14, 191, 765, 792, 865
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 878
Bse1I ACTGG 1 cut(s) 967
Bse3DI GCAATG 4 cut(s) 118, 238, 913, 927
BseBI CCWGG 3 cut(s) 329, 639, 866
BseDI CCNNGG 5 cut(s) 14, 191, 765, 792, 865
BseLI CCNNNNNNNGG 2 cut(s) 244, 878
BseMI GCAATG 4 cut(s) 118, 238, 913, 927
BseMII CTCAG 2 cut(s) 448, 740
BseNI ACTGG 1 cut(s) 967
BseRI GAGGAG 1 cut(s) 693
BseXI GCAGC 2 cut(s) 274, 742
BshFI GGCC 3 cut(s) 211, 355, 624
BsiHKAI GWGCWC 2 cut(s) 611, 646
BsiSI CCGG 3 cut(s) 655, 743, 756
BslFI GGGAC 2 cut(s) 100, 173
BslI CCNNNNNNNGG 2 cut(s) 244, 878
BsmAI GTCTC 1 cut(s) 714
BsmBI CGTCTC 1 cut(s) 714
BsmFI GGGAC 2 cut(s) 100, 173
BsmI GAATGC 2 cut(s) 787, 948
BsnI GGCC 3 cut(s) 211, 355, 624
Bsp119I TTCGAA 2 cut(s) 135, 318
Bsp1286I GDGCHC 2 cut(s) 611, 646
Bsp143I GATC 2 cut(s) 979, 1018
Bsp19I CCATGG 1 cut(s) 765
BspACI CCGC 5 cut(s) 356, 667, 719, 722, 785
BspANI GGCC 3 cut(s) 211, 355, 624
BspCNI CTCAG 2 cut(s) 447, 739
BspLI GGNNCC 2 cut(s) 660, 870
BspMI ACCTGC 1 cut(s) 505
BspT104I TTCGAA 2 cut(s) 135, 318
BsrDI GCAATG 4 cut(s) 118, 238, 913, 927
BsrI ACTGG 1 cut(s) 967
BssECI CCNNGG 5 cut(s) 14, 191, 765, 792, 865
BssMI GATC 2 cut(s) 979, 1018
BssT1I CCWWGG 4 cut(s) 14, 191, 765, 792
Bst2UI CCWGG 3 cut(s) 329, 639, 866
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 3 cut(s) 134, 565, 574
BstAPI GCANNNNNTGC 1 cut(s) 96
BstBI TTCGAA 2 cut(s) 135, 318
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 4 cut(s) 197, 434, 726, 816
BstDSI CCRYGG 1 cut(s) 765
BstEII GGTNACC 1 cut(s) 787
BstKTI GATC 2 cut(s) 982, 1021
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 2 cut(s) 979, 1018
BstMWI GCNNNNNNNGC 8 cut(s) 96, 208, 606, 727, 730, 739, 847, 888
BstNI CCWGG 3 cut(s) 329, 639, 866
BstPI GGTNACC 1 cut(s) 787
BstSCI CCNGG 5 cut(s) 327, 637, 654, 755, 864
BstSFI CTRYAG 1 cut(s) 291
BstV1I GCAGC 2 cut(s) 274, 742
BstV2I GAAGAC 2 cut(s) 640, 666
BstX2I RGATCY 1 cut(s) 1018
BstYI RGATCY 1 cut(s) 1018
BsuRI GGCC 3 cut(s) 211, 355, 624
BtgI CCRYGG 1 cut(s) 765
BtsIMutI CAGTG 1 cut(s) 468
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 35
CaiI CAGNNNCTG 1 cut(s) 80
Cfr13I GGNCC 2 cut(s) 623, 869
CsiI ACCWGGT 1 cut(s) 637
Csp6I GTAC 1 cut(s) 832
CviAII CATG 4 cut(s) 46, 223, 766, 778
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 4 cut(s) 197, 434, 726, 816
DpnI GATC 2 cut(s) 981, 1020
DpnII GATC 2 cut(s) 979, 1018
EaeI YGGCCR 1 cut(s) 353
Eam1104I CTCTTC 3 cut(s) 134, 565, 574
EarI CTCTTC 3 cut(s) 134, 565, 574
EciI GGCGGA 1 cut(s) 708
Ecl136II GAGCTC 1 cut(s) 609
Eco130I CCWWGG 4 cut(s) 14, 191, 765, 792
Eco24I GRGCYC 1 cut(s) 611
Eco47I GGWCC 1 cut(s) 869
Eco53kI GAGCTC 1 cut(s) 609
Eco57I CTGAAG 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 787
EcoICRI GAGCTC 1 cut(s) 609
EcoO109I RGGNCCY 2 cut(s) 623, 869
EcoO65I GGTNACC 1 cut(s) 787
EcoRI GAATTC 2 cut(s) 314, 1011
EcoRII CCWGG 3 cut(s) 327, 637, 864
EcoT14I CCWWGG 4 cut(s) 14, 191, 765, 792
EcoT38I GRGCYC 1 cut(s) 611
ErhI CCWWGG 4 cut(s) 14, 191, 765, 792
Esp3I CGTCTC 1 cut(s) 714
FaeI CATG 4 cut(s) 49, 226, 769, 781
FaiI YATR 9 cut(s) 47, 92, 224, 554, 588, 602, 767, 779, 957
FaqI GGGAC 2 cut(s) 100, 173
FatI CATG 4 cut(s) 45, 222, 765, 777
FauI CCCGC 1 cut(s) 674
FbaI TGATCA 1 cut(s) 979
Fnu4HI GCNGC 4 cut(s) 288, 356, 722, 731
FriOI GRGCYC 1 cut(s) 611
Fsp4HI GCNGC 4 cut(s) 288, 356, 722, 731
FspBI CTAG 2 cut(s) 234, 1016
GluI GCNGC 4 cut(s) 288, 356, 722, 731
HaeIII GGCC 3 cut(s) 211, 355, 624
HapII CCGG 3 cut(s) 655, 743, 756
Hin1II CATG 4 cut(s) 49, 226, 769, 781
HindIII AAGCTT 2 cut(s) 35, 848
HinfI GANTC 2 cut(s) 113, 823
HpaII CCGG 3 cut(s) 655, 743, 756
HphI GGTGA 2 cut(s) 495, 799
Hpy188I TCNGA 4 cut(s) 437, 632, 970, 1010
Hpy188III TCNNGA 4 cut(s) 802, 827, 1016, 1022
HpyAV CCTTC 7 cut(s) 314, 484, 614, 657, 708, 723, 805
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4V TGCA 6 cut(s) 33, 90, 243, 483, 500, 959
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 208, 606, 727, 730, 739, 847, 888
HpyF3I CTNAG 4 cut(s) 197, 434, 726, 816
Hsp92II CATG 4 cut(s) 49, 226, 769, 781
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 2 cut(s) 979, 1018
LmnI GCTCC 3 cut(s) 606, 614, 649
Lsp1109I GCAGC 2 cut(s) 274, 742
LweI GCATC 4 cut(s) 341, 476, 492, 509
MabI ACCWGGT 1 cut(s) 637
MaeI CTAG 2 cut(s) 234, 1016
MaeIII GTNAC 3 cut(s) 82, 298, 787
MalI GATC 2 cut(s) 981, 1020
MboI GATC 2 cut(s) 979, 1018
MflI RGATCY 1 cut(s) 1018
MhlI GDGCHC 2 cut(s) 611, 646
MluCI AATT 6 cut(s) 54, 308, 314, 428, 1003, 1011
MlyI GAGTC 1 cut(s) 122
MmeI TCCRAC 2 cut(s) 406, 919
MseI TTAA 2 cut(s) 27, 278
MslI CAYNNNNRTG 1 cut(s) 455
MspI CCGG 3 cut(s) 655, 743, 756
MspR9I CCNGG 5 cut(s) 329, 639, 656, 757, 866
Mva1269I GAATGC 2 cut(s) 787, 948
MvaI CCWGG 3 cut(s) 329, 639, 866
MwoI GCNNNNNNNGC 8 cut(s) 96, 208, 606, 727, 730, 739, 847, 888
NciI CCSGG 2 cut(s) 656, 757
NcoI CCATGG 1 cut(s) 765
NdeII GATC 2 cut(s) 979, 1018
NlaIII CATG 4 cut(s) 49, 226, 769, 781
NlaIV GGNNCC 2 cut(s) 660, 870
NmuCI GTSAC 3 cut(s) 82, 298, 787
NspV TTCGAA 2 cut(s) 135, 318
PaqCI CACCTGC 1 cut(s) 505
PctI GAATGC 2 cut(s) 787, 948
PfeI GAWTC 1 cut(s) 823
PfoI TCCNGGA 1 cut(s) 654
PkrI GCNGC 4 cut(s) 289, 357, 723, 732
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PpuMI RGGWCCY 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 611
Psp5II RGGWCCY 1 cut(s) 869
Psp6I CCWGG 3 cut(s) 327, 637, 864
PspEI GGTNACC 1 cut(s) 787
PspGI CCWGG 3 cut(s) 327, 637, 864
PspN4I GGNNCC 2 cut(s) 660, 870
PspPI GGNCC 2 cut(s) 623, 869
PspPPI RGGWCCY 1 cut(s) 869
PstNI CAGNNNCTG 1 cut(s) 80
PsuI RGATCY 1 cut(s) 1018
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 611
SaqAI TTAA 2 cut(s) 27, 278
SatI GCNGC 4 cut(s) 288, 356, 722, 731
Sau3AI GATC 2 cut(s) 979, 1018
Sau96I GGNCC 2 cut(s) 623, 869
ScaI AGTACT 1 cut(s) 833
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 5 cut(s) 329, 639, 656, 757, 866
SduI GDGCHC 2 cut(s) 611, 646
SexAI ACCWGGT 1 cut(s) 637
SfaNI GCATC 4 cut(s) 341, 476, 492, 509
SfcI CTRYAG 1 cut(s) 291
SfuI TTCGAA 2 cut(s) 135, 318
SinI GGWCC 1 cut(s) 869
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 1 cut(s) 851
SmoI CTYRAG 1 cut(s) 851
Sse9I AATT 6 cut(s) 54, 308, 314, 428, 1003, 1011
SsiI CCGC 5 cut(s) 356, 667, 719, 722, 785
SspMI CTAG 2 cut(s) 234, 1016
SstI GAGCTC 1 cut(s) 611
StyD4I CCNGG 5 cut(s) 327, 637, 654, 755, 864
StyI CCWWGG 4 cut(s) 14, 191, 765, 792
TaaI ACNGT 1 cut(s) 295
TaqI TCGA 3 cut(s) 135, 318, 737
TasI AATT 6 cut(s) 54, 308, 314, 428, 1003, 1011
TatI WGTACW 1 cut(s) 831
TauI GCSGC 2 cut(s) 358, 724
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 2 cut(s) 27, 278
Tru9I TTAA 2 cut(s) 27, 278
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 3 cut(s) 82, 298, 787
TseI GCWGC 2 cut(s) 287, 730
Tsp45I GTSAC 3 cut(s) 82, 298, 787
TspDTI ATGAA 1 cut(s) 794
TspGWI ACGGA 2 cut(s) 125, 696
TspRI CASTG 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 869
XapI RAATTY 4 cut(s) 54, 314, 428, 1011
XbaI TCTAGA 1 cut(s) 1015
XspI CTAG 2 cut(s) 234, 1016
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.