pycom05g02980
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008234
Physical Location & Seq
Forward (+)
3478414 .. 3483371
4958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02980.8

Sequence Viewer

Length: 789 bp
ATGGAGAGAAAGGTTGGGAATTCAGTTTGTCTCTGGTTGATCTTGGTAGCTCATCCACTATGGATGACAATGGTGCTTGCTAATATGGAAGGTGATGCCTTGCATAATCTAAGAACCAACTTGGAGGACCCCAACAATGTCCTGCAAAGTTGGGATCCTACTCTTGTGAACCCTTGTACGTGGTTTCATGTTACATGCAACAATGAAAATAGCGTCATAAGAGTTGATCTTGAAAACGCAGCCTTGTCTGGTCAACTTGTTCCGCAGCTTGGCCTTCTCAAGAATTTACAGTATCTGGAACTCTACAGTAATAACATAAGTGGACCAATTCCTAGGGAACTGGGGAACCTAACCACCTTGGTGAGCTTGGATCTTTATTTGAATAGTTTTTCAGGTCCAATCCCAGACACCTTGGGCAAGCTGTCAAAACTACGATTCCTGCGGCTTAACAACAACAGCTTGGCGGGTCCGATTCCCATGTCATTGACTAATATCTCTTCACTTCAAGTACTGGATCTATCAAATAATCGTCTCTCAGGCGTAGTTCCAGACAATGGCTCCTTTTCTTTATTCACTCCCATAAGTTTTGCTAACAACATGAATCTATGTGGCCCAGTAACTGGTCGCCCCTGCCCACGATCTCCTCCATTTTCACCTCCCCCACCATTTGTCCCACCACCGCCAATTTCAATACCAGGGATAGTTGCCCTTGAGATGTTTTGCAAGTTGGATCTCCTCTCGACTGCAATTTATAGAGTAAACTTCGTACCAAGTTGCTTATTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007030 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009908 GO:0009987 GO:0010033 GO:0010152 GO:0010154 GO:0010227 GO:0010256 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0021700 GO:0022402 GO:0022414 GO:0023052 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033612 GO:0033993 GO:0034293 GO:0036211 GO:0038023 GO:0042175 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046777 GO:0048229 GO:0048236 GO:0048316 GO:0048367 GO:0048437 GO:0048545 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0098827 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

263

Amino Acids

28.76

Weight (kDa)

5.59

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 554, 620
AciI CCGC 4 cut(s) 263, 442, 464, 680
AclWI GGATC 5 cut(s) 149, 162, 378, 522, 738
AcsI RAATTY 2 cut(s) 19, 283
AfaI GTAC 3 cut(s) 178, 510, 768
AfiI CCNNNNNNNGG 3 cut(s) 269, 554, 620
AgsI TTSAA 4 cut(s) 233, 382, 506, 690
AjnI CCWGG 1 cut(s) 694
AleI CACNNNNGTG 1 cut(s) 359
AluBI AGCT 5 cut(s) 50, 268, 366, 421, 459
AluI AGCT 5 cut(s) 50, 268, 366, 421, 459
Alw26I GTCTC 2 cut(s) 35, 536
AlwI GGATC 5 cut(s) 149, 162, 378, 522, 738
AlwNI CAGNNNCTG 2 cut(s) 295, 620
AoxI GGCC 2 cut(s) 271, 610
ApeKI GCWGC 2 cut(s) 239, 265
ApoI RAATTY 2 cut(s) 19, 283
AspA2I CCTAGG 1 cut(s) 332
AspS9I GGNCC 5 cut(s) 127, 323, 395, 467, 611
AsuHPI GGTGA 3 cut(s) 104, 373, 645
AvaII GGWCC 4 cut(s) 127, 323, 395, 467
AvrII CCTAGG 1 cut(s) 332
BamHI GGATCC 1 cut(s) 154
BbvI GCAGC 2 cut(s) 251, 277
BciT130I CCWGG 1 cut(s) 696
BcoDI GTCTC 2 cut(s) 35, 536
BfaI CTAG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 304
BisI GCNGC 3 cut(s) 240, 266, 443
BlnI CCTAGG 1 cut(s) 332
BlsI GCNGC 3 cut(s) 241, 267, 444
BmcAI AGTACT 1 cut(s) 510
Bme1390I CCNGG 1 cut(s) 696
Bme18I GGWCC 4 cut(s) 127, 323, 395, 467
BmgT120I GGNCC 5 cut(s) 127, 323, 395, 467, 611
BmiI GGNNCC 5 cut(s) 129, 156, 347, 468, 559
BmrFI CCNGG 1 cut(s) 696
BmrI ACTGGG 2 cut(s) 350, 608
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 2 cut(s) 350, 608
BpuEI CTTGAG 2 cut(s) 263, 731
BsaAI YACGTR 1 cut(s) 180
BsaJI CCNNGG 4 cut(s) 332, 357, 411, 695
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 554, 620
Bse1I ACTGG 4 cut(s) 345, 516, 614, 625
BseBI CCWGG 1 cut(s) 696
BseDI CCNNGG 4 cut(s) 332, 357, 411, 695
BseGI GGATG 2 cut(s) 52, 69
BseLI CCNNNNNNNGG 3 cut(s) 269, 554, 620
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 4 cut(s) 345, 516, 614, 625
BseRI GAGGAG 2 cut(s) 633, 725
BseXI GCAGC 2 cut(s) 251, 277
BshFI GGCC 2 cut(s) 273, 612
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 3 cut(s) 269, 554, 620
BsmAI GTCTC 2 cut(s) 35, 536
BsmBI CGTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 2 cut(s) 273, 612
Bsp143I GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
BspACI CCGC 4 cut(s) 263, 442, 464, 680
BspANI GGCC 2 cut(s) 273, 612
BspCNI CTCAG 1 cut(s) 548
BspLI GGNNCC 5 cut(s) 129, 156, 347, 468, 559
BspPI GGATC 5 cut(s) 149, 162, 378, 522, 738
BsrI ACTGG 4 cut(s) 345, 516, 614, 625
BssECI CCNNGG 4 cut(s) 332, 357, 411, 695
BssMI GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
BssT1I CCWWGG 3 cut(s) 332, 357, 411
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 291, 308
Bst6I CTCTTC 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 78, 419
BstDEI CTNAG 2 cut(s) 110, 535
BstF5I GGATG 2 cut(s) 52, 69
BstKTI GATC 7 cut(s) 42, 157, 229, 373, 517, 641, 733
BstMAI GTCTC 2 cut(s) 35, 536
BstMBI GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
BstNI CCWGG 1 cut(s) 696
BstNSI RCATGY 1 cut(s) 198
BstSCI CCNGG 1 cut(s) 694
BstSFI CTRYAG 1 cut(s) 304
BstV1I GCAGC 2 cut(s) 251, 277
BstX2I RGATCY 4 cut(s) 154, 370, 514, 730
BstYI RGATCY 4 cut(s) 154, 370, 514, 730
BsuRI GGCC 2 cut(s) 273, 612
BtsCI GGATG 2 cut(s) 52, 69
Cac8I GCNNGC 2 cut(s) 78, 419
CaiI CAGNNNCTG 2 cut(s) 295, 620
Cfr13I GGNCC 5 cut(s) 127, 323, 395, 467, 611
CseI GACGC 1 cut(s) 202
Csp6I GTAC 3 cut(s) 177, 509, 767
CviAII CATG 4 cut(s) 188, 195, 478, 598
CviQI GTAC 3 cut(s) 177, 509, 767
DdeI CTNAG 2 cut(s) 110, 535
DpnI GATC 7 cut(s) 41, 156, 228, 372, 516, 640, 732
DpnII GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
Eam1104I CTCTTC 1 cut(s) 502
EarI CTCTTC 1 cut(s) 502
Eco130I CCWWGG 3 cut(s) 332, 357, 411
Eco47I GGWCC 4 cut(s) 127, 323, 395, 467
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 694
EcoT14I CCWWGG 3 cut(s) 332, 357, 411
ErhI CCWWGG 3 cut(s) 332, 357, 411
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 4 cut(s) 191, 198, 481, 601
FaqI GGGAC 1 cut(s) 656
FatI CATG 4 cut(s) 187, 194, 477, 597
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 3 cut(s) 240, 266, 443
FokI GGATG 2 cut(s) 39, 76
Fsp4HI GCNGC 3 cut(s) 240, 266, 443
FspBI CTAG 1 cut(s) 333
GluI GCNGC 3 cut(s) 240, 266, 443
HaeIII GGCC 2 cut(s) 273, 612
HgaI GACGC 1 cut(s) 202
Hin1II CATG 4 cut(s) 191, 198, 481, 601
HincII GTYRAC 1 cut(s) 254
HindII GTYRAC 1 cut(s) 254
HinfI GANTC 3 cut(s) 435, 472, 601
HphI GGTGA 3 cut(s) 104, 373, 645
Hpy166II GTNNAC 4 cut(s) 169, 254, 323, 760
Hpy188I TCNGA 1 cut(s) 471
Hpy188III TCNNGA 5 cut(s) 230, 280, 296, 548, 739
Hpy8I GTNNAC 4 cut(s) 169, 254, 323, 760
HpyAV CCTTC 2 cut(s) 83, 284
HpyCH4III ACNGT 2 cut(s) 291, 308
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 5 cut(s) 103, 145, 198, 723, 746
HpyF3I CTNAG 2 cut(s) 110, 535
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 4 cut(s) 191, 198, 481, 601
Kzo9I GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
LmnI GCTCC 1 cut(s) 563
Lsp1109I GCAGC 2 cut(s) 251, 277
LweI GCATC 1 cut(s) 85
MaeI CTAG 1 cut(s) 333
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 2 cut(s) 190, 616
MalI GATC 7 cut(s) 41, 156, 228, 372, 516, 640, 732
MboI GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
MboII GAAGA 1 cut(s) 489
MflI RGATCY 4 cut(s) 154, 370, 514, 730
MluCI AATT 5 cut(s) 19, 283, 327, 684, 747
MmeI TCCRAC 1 cut(s) 708
MnlI CCTC 4 cut(s) 118, 654, 666, 746
MseI TTAA 1 cut(s) 447
MslI CAYNNNNRTG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 696
MvaI CCWGG 1 cut(s) 696
NdeII GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
NlaIII CATG 4 cut(s) 191, 198, 481, 601
NlaIV GGNNCC 5 cut(s) 129, 156, 347, 468, 559
NspI RCATGY 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 359
PfeI GAWTC 3 cut(s) 435, 472, 601
PflMI CCANNNNNTGG 2 cut(s) 554, 620
PkrI GCNGC 3 cut(s) 241, 267, 444
Ppu21I YACGTR 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 127
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 694
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 5 cut(s) 129, 156, 347, 468, 559
PspPI GGNCC 5 cut(s) 127, 323, 395, 467, 611
PspPPI RGGWCCY 1 cut(s) 127
PstNI CAGNNNCTG 2 cut(s) 295, 620
PsuI RGATCY 4 cut(s) 154, 370, 514, 730
RsaI GTAC 3 cut(s) 178, 510, 768
RsaNI GTAC 3 cut(s) 177, 509, 767
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 3 cut(s) 240, 266, 443
Sau3AI GATC 7 cut(s) 39, 154, 226, 370, 514, 638, 730
Sau96I GGNCC 5 cut(s) 127, 323, 395, 467, 611
ScaI AGTACT 1 cut(s) 510
ScrFI CCNGG 1 cut(s) 696
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 304
SinI GGWCC 4 cut(s) 127, 323, 395, 467
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 278, 710
SmoI CTYRAG 2 cut(s) 278, 710
Sse9I AATT 5 cut(s) 19, 283, 327, 684, 747
SsiI CCGC 4 cut(s) 263, 442, 464, 680
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 694
StyI CCWWGG 3 cut(s) 332, 357, 411
TaaI ACNGT 2 cut(s) 291, 308
TaiI ACGT 1 cut(s) 182
TaqI TCGA 1 cut(s) 740
TasI AATT 5 cut(s) 19, 283, 327, 684, 747
TatI WGTACW 1 cut(s) 508
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 3 cut(s) 435, 472, 601
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TseI GCWGC 2 cut(s) 239, 265
TspDTI ATGAA 3 cut(s) 176, 219, 614
Van91I CCANNNNNTGG 2 cut(s) 554, 620
VpaK11BI GGWCC 4 cut(s) 127, 323, 395, 467
XapI RAATTY 2 cut(s) 19, 283
XceI RCATGY 1 cut(s) 198
XmaJI CCTAGG 1 cut(s) 332
XspI CTAG 1 cut(s) 333
ZrmI AGTACT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.