pycom10g03010
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008234
Physical Location & Seq
Forward (+)
3377216 .. 3378956
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g03010.2

Sequence Viewer

Length: 834 bp
ATGATCAGCATGGCTGTGCATCGAAATCTTCTTCGGTTACGTGGGTTCTGTATGACACCAACTGAACGGTTACTTGTCTATCCTTACATGGCTAACGGGAGTGTTGCCTCATGTTTAAGAGAACGCCCGCCAAACCAACCACCTCTTGATTGGCCAACTCGGAAGCGAATTGCACTGGGATCTACAAGGGGTCTTTCTTATTTGCATGATCACTGTGACCTGAAGATCATTCACCGTGATGTGAAAGCTGCAAACATTTTGCTGGATGAGGAGTTTGAGGCTGTTGTTGGAGACTTTGGGTTGGCTAAACTTATGGACTACAAAGATACCCACGTCACTACTGCTGTACGTGGCACAATTGGACATATAGCTCCAGAGTACCTGTCTACCGGGAAGTCATCTGAGAAAACTGATGTTTTCGGTTATGGTATCATGCTTCTGGAGCTTATTACTGGTCAGAGGGCTTTTGATCTTGCTCGCCTTGCAAATGATGATGATGTCATGTTGCTTGATTGGGTGAAAGGACTATTGAAGGAGAAAAAGCTAGAAATGCTGGTTGATCGATCTGATCTCCAGAGTAATTATGTAGAAGCTGAGGTAGAGCAGCTAATTCAAGTTGCACTGCTCTGCACACAAGGCTCCCCAATGGACCGGCCTAAGATGTCGGAAGTTGTAAGGATGCTTGAAGGTGATGGCTTGGCAGAAAGATGGGATGAGTGGCAAAAGGTCGAAGTGCTTCGCCAAGAAGTGGAGTTAGCTCCTCACCCGAATTCAGATTGGATTGTTGACTCAACAGAAAATTTGCATGCAGTTGAGTTATCCGGTCCAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007030 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009908 GO:0009987 GO:0010033 GO:0010152 GO:0010154 GO:0010227 GO:0010256 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0021700 GO:0022402 GO:0022414 GO:0023052 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033612 GO:0033993 GO:0034293 GO:0036211 GO:0038023 GO:0042175 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046777 GO:0048229 GO:0048236 GO:0048316 GO:0048367 GO:0048437 GO:0048545 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0098827 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

278

Amino Acids

31.35

Weight (kDa)

5.25

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 748
AccI GTMKAC 1 cut(s) 386
AciI CCGC 1 cut(s) 128
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 2 cut(s) 769, 799
AcuI CTGAAG 1 cut(s) 242
AfaI GTAC 2 cut(s) 348, 380
AfiI CCNNNNNNNGG 2 cut(s) 748, 828
AgsI TTSAA 3 cut(s) 532, 614, 686
AjiI CACGTC 1 cut(s) 334
AluBI AGCT 7 cut(s) 248, 371, 445, 544, 593, 607, 758
AluI AGCT 7 cut(s) 248, 371, 445, 544, 593, 607, 758
Alw26I GTCTC 1 cut(s) 285
AlwI GGATC 1 cut(s) 187
AoxI GGCC 2 cut(s) 152, 653
ApeKI GCWGC 2 cut(s) 248, 604
ApoI RAATTY 2 cut(s) 769, 799
Asp700I GAANNNNTTC 1 cut(s) 735
AspS9I GGNCC 2 cut(s) 649, 824
AsuC2I CCSGG 1 cut(s) 391
AsuHPI GGTGA 4 cut(s) 224, 529, 701, 755
AvaII GGWCC 2 cut(s) 649, 824
BaeI ACNNNNGTAYC 2 cut(s) 318, 351
BalI TGGCCA 1 cut(s) 154
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 2 cut(s) 235, 616
BccI CCATC 2 cut(s) 686, 702
BclI TGATCA 2 cut(s) 3, 208
BcnI CCSGG 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 285
BfaI CTAG 1 cut(s) 545
BisI GCNGC 2 cut(s) 249, 605
BlsI GCNGC 2 cut(s) 250, 606
Bme1390I CCNGG 1 cut(s) 391
Bme18I GGWCC 2 cut(s) 649, 824
BmgBI CACGTC 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 649, 824
BmiI GGNNCC 1 cut(s) 640
BmrFI CCNGG 1 cut(s) 391
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 28, 669
BmuI ACTGGG 1 cut(s) 185
BpmI CTGGAG 3 cut(s) 357, 461, 557
Bpu10I CCTNAGC 1 cut(s) 594
BpuMI CCSGG 1 cut(s) 391
Bsa29I ATCGAT 1 cut(s) 562
BsaAI YACGTR 2 cut(s) 41, 350
BsaJI CCNNGG 1 cut(s) 827
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 828
Bse118I RCCGGY 1 cut(s) 651
Bse1I ACTGG 2 cut(s) 180, 457
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 827
BseGI GGATG 3 cut(s) 271, 684, 718
BseLI CCNNNNNNNGG 2 cut(s) 748, 828
BseMII CTCAG 2 cut(s) 393, 585
BseNI ACTGG 2 cut(s) 180, 457
BseRI GAGGAG 2 cut(s) 284, 750
BseXI GCAGC 2 cut(s) 235, 616
BsgI GTGCAG 1 cut(s) 613
BshFI GGCC 2 cut(s) 154, 655
BshVI ATCGAT 1 cut(s) 562
BsiSI CCGG 3 cut(s) 390, 652, 822
BslI CCNNNNNNNGG 2 cut(s) 748, 828
BsmAI GTCTC 1 cut(s) 285
BsnI GGCC 2 cut(s) 154, 655
Bsp143I GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
BspACI CCGC 1 cut(s) 128
BspANI GGCC 2 cut(s) 154, 655
BspCNI CTCAG 2 cut(s) 394, 586
BspDI ATCGAT 1 cut(s) 562
BspLI GGNNCC 1 cut(s) 640
BspPI GGATC 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 651
BsrI ACTGG 2 cut(s) 180, 457
BssAI RCCGGY 1 cut(s) 651
BssECI CCNNGG 1 cut(s) 827
BssMI GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
BssT1I CCWWGG 1 cut(s) 827
Bst4CI ACNGT 3 cut(s) 69, 215, 236
BstBAI YACGTR 2 cut(s) 41, 350
BstC8I GCNNGC 3 cut(s) 128, 478, 807
BstDEI CTNAG 3 cut(s) 402, 594, 657
BstF5I GGATG 3 cut(s) 271, 684, 718
BstKTI GATC 8 cut(s) 6, 182, 211, 228, 472, 562, 566, 571
BstMAI GTCTC 1 cut(s) 285
BstMBI GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
BstMWI GCNNNNNNNGC 4 cut(s) 442, 482, 550, 636
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 1 cut(s) 389
BstV1I GCAGC 2 cut(s) 235, 616
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 562
BsuRI GGCC 2 cut(s) 154, 655
BsuTUI ATCGAT 1 cut(s) 562
BtrI CACGTC 1 cut(s) 334
BtsCI GGATG 3 cut(s) 271, 684, 718
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 3 cut(s) 173, 211, 620
Cac8I GCNNGC 3 cut(s) 128, 478, 807
Cfr10I RCCGGY 1 cut(s) 651
Cfr13I GGNCC 2 cut(s) 649, 824
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 2 cut(s) 347, 379
CviAII CATG 7 cut(s) 10, 88, 111, 206, 433, 502, 806
CviQI GTAC 2 cut(s) 347, 379
DdeI CTNAG 3 cut(s) 402, 594, 657
DpnI GATC 8 cut(s) 5, 181, 210, 227, 471, 561, 565, 570
DpnII GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
EaeI YGGCCR 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 827
Eco47I GGWCC 2 cut(s) 649, 824
Eco57I CTGAAG 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 769
EcoT14I CCWWGG 1 cut(s) 827
ErhI CCWWGG 1 cut(s) 827
FaeI CATG 7 cut(s) 13, 91, 114, 209, 436, 505, 809
FatI CATG 7 cut(s) 9, 87, 110, 205, 432, 501, 805
FauI CCCGC 1 cut(s) 135
FbaI TGATCA 2 cut(s) 3, 208
FblI GTMKAC 1 cut(s) 386
Fnu4HI GCNGC 2 cut(s) 249, 605
FokI GGATG 3 cut(s) 278, 691, 725
Fsp4HI GCNGC 2 cut(s) 249, 605
FspBI CTAG 1 cut(s) 545
GluI GCNGC 2 cut(s) 249, 605
GsuI CTGGAG 3 cut(s) 357, 461, 557
HaeIII GGCC 2 cut(s) 154, 655
HapII CCGG 3 cut(s) 390, 652, 822
Hin1II CATG 7 cut(s) 13, 91, 114, 209, 436, 505, 809
HincII GTYRAC 1 cut(s) 787
HindII GTYRAC 1 cut(s) 787
HinfI GANTC 1 cut(s) 788
HpaII CCGG 3 cut(s) 390, 652, 822
HphI GGTGA 4 cut(s) 224, 529, 701, 755
Hpy166II GTNNAC 2 cut(s) 387, 787
Hpy188I TCNGA 6 cut(s) 162, 403, 459, 568, 667, 775
Hpy188III TCNNGA 4 cut(s) 146, 374, 440, 574
Hpy8I GTNNAC 2 cut(s) 387, 787
HpyAV CCTTC 2 cut(s) 526, 680
HpyCH4III ACNGT 3 cut(s) 69, 215, 236
HpyCH4IV ACGT 3 cut(s) 40, 333, 349
HpyCH4V TGCA 9 cut(s) 19, 173, 205, 251, 485, 620, 630, 805, 809
HpyF10VI GCNNNNNNNGC 4 cut(s) 442, 482, 550, 636
HpyF3I CTNAG 3 cut(s) 402, 594, 657
HpySE526I ACGT 3 cut(s) 40, 333, 349
Hsp92II CATG 7 cut(s) 13, 91, 114, 209, 436, 505, 809
Ksp22I TGATCA 2 cut(s) 3, 208
Kzo9I GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
LmnI GCTCC 4 cut(s) 376, 442, 644, 763
Lsp1109I GCAGC 2 cut(s) 235, 616
LweI GCATC 2 cut(s) 28, 669
MaeI CTAG 1 cut(s) 545
MaeII ACGT 3 cut(s) 40, 333, 349
MaeIII GTNAC 4 cut(s) 36, 69, 215, 334
MalI GATC 8 cut(s) 5, 181, 210, 227, 471, 561, 565, 570
MboI GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
MboII GAAGA 3 cut(s) 20, 23, 235
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 179
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 6 cut(s) 168, 357, 580, 609, 769, 799
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 2 cut(s) 268, 645
MnlI CCTC 7 cut(s) 118, 153, 262, 271, 453, 589, 771
Mox20I TGGCCA 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 735
MscI TGGCCA 1 cut(s) 154
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 14, 237
Msp20I TGGCCA 1 cut(s) 154
MspI CCGG 3 cut(s) 390, 652, 822
MspR9I CCNGG 1 cut(s) 391
MunI CAATTG 1 cut(s) 357
MwoI GCNNNNNNNGC 4 cut(s) 442, 482, 550, 636
NciI CCSGG 1 cut(s) 391
NdeII GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
NlaIII CATG 7 cut(s) 13, 91, 114, 209, 436, 505, 809
NlaIV GGNNCC 1 cut(s) 640
NmuCI GTSAC 2 cut(s) 215, 334
NspI RCATGY 1 cut(s) 809
PaeI GCATGC 1 cut(s) 809
PdmI GAANNNNTTC 1 cut(s) 735
PflMI CCANNNNNTGG 1 cut(s) 748
PkrI GCNGC 2 cut(s) 250, 606
PleI GAGTC 1 cut(s) 782
PpsI GAGTC 1 cut(s) 782
Ppu21I YACGTR 2 cut(s) 41, 350
PspN4I GGNNCC 1 cut(s) 640
PspPI GGNCC 2 cut(s) 649, 824
PsuI RGATCY 1 cut(s) 179
RsaI GTAC 2 cut(s) 348, 380
RsaNI GTAC 2 cut(s) 347, 379
RseI CAYNNNNRTG 2 cut(s) 14, 237
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 2 cut(s) 249, 605
Sau3AI GATC 8 cut(s) 3, 179, 208, 225, 469, 559, 563, 568
Sau96I GGNCC 2 cut(s) 649, 824
SchI GAGTC 1 cut(s) 782
ScrFI CCNGG 1 cut(s) 391
SfaNI GCATC 2 cut(s) 28, 669
SinI GGWCC 2 cut(s) 649, 824
SmiMI CAYNNNNRTG 2 cut(s) 14, 237
SphI GCATGC 1 cut(s) 809
Sse9I AATT 6 cut(s) 168, 357, 580, 609, 769, 799
SsiI CCGC 1 cut(s) 128
SspMI CTAG 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 389
StyI CCWWGG 1 cut(s) 827
TaaI ACNGT 3 cut(s) 69, 215, 236
TaiI ACGT 3 cut(s) 43, 336, 352
TaqI TCGA 3 cut(s) 22, 562, 729
TasI AATT 6 cut(s) 168, 357, 580, 609, 769, 799
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 3 cut(s) 180, 218, 627
TseFI GTSAC 2 cut(s) 215, 334
TseI GCWGC 2 cut(s) 248, 604
Tsp45I GTSAC 2 cut(s) 215, 334
TspRI CASTG 3 cut(s) 180, 218, 627
Van91I CCANNNNNTGG 1 cut(s) 748
VpaK11BI GGWCC 2 cut(s) 649, 824
XapI RAATTY 2 cut(s) 769, 799
XceI RCATGY 1 cut(s) 809
XcmI CCANNNNNNNNNTGG 1 cut(s) 147
XmiI GTMKAC 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.