pycom10g03000
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0008234
Physical Location & Seq
Forward (+)
3372227 .. 3377147
4921 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g03000.6

Sequence Viewer

Length: 987 bp
ATGGAGAGAAAGGTTGGGAATTCAGTTTGTCTATGGTTCATCTTGGTGGCTCATCCACTATGGATGACAATGGTGCTTGCTAATATGGAAGGTGATGCCTTGCATACTCTAAGGACCAACTTGGAGGACCCCAACAATGTCCTGCAAAGTTGGGATCGATCTACTATTGTGAACCCTTGTACGTGGTTTCATGTTACATGCAACAATGACAATAGCGTCATAAGAGTTGATCTTGGAAATGCAGCCTTGTCTGGTCAACTTGTTCCACAGCTTGGCCTTCTCAAGAATTTACAATATTTGGAGCTCTACAGTAATAACATGAGTGGACCAATTCCTAGTGAACTGGGGAACCTAACCAGCTTGGTGAGCTTGGATCTTTATTTGAATAGTTTTTCAGGTCTAATCCCAGGCACCTTGGGCAAGCTGTCAAAACTGCGATTCCTCCGGCTTAACAACAACAGCTTGGCGGGTCCGATTCCCATGTCTTTGACTAATATCTCTTCACTTCAAGTACTGGATCTATCAAATAATCGTCTCTCAGGAGTAGTTCCAGACAATGGCTCCTTTTCTTTATTCACTCCCATAAGTTTTGCTAACAACATGGATCTGTGTGGCCCAGTAACTGGTCGCCCCTGCCCAGGATCTCCTCCATTTTCACCTCCCCCACCTTTTGTTCCACCACCGCCAATTTCAACACCAGGAGGTAATGGTGCCACTGGGGCTATTGCTGGTGGAGTTGCCGCTGGTGCTGCTTTACTATTTGCTGCCCCTGCAATTGCATTTGCATGGTGGCGCCGGAGGAAGCCGCAAGAATTTTTCTTTGATGTACCTGGATCGATCACGAGCCTGAAGTACATCTTGGGCAGCTCAAGAGGTTTTCTTTGCGAGAATTACAAGTTGCAACGGATAGTTTTAGCAACAAAAACATTCTGGGGAGAGGTGGATTTGGTAAGGTCTACAAGGGGCGCCTTGCAGATGGTTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007030 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009908 GO:0009987 GO:0010033 GO:0010152 GO:0010154 GO:0010227 GO:0010256 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0021700 GO:0022402 GO:0022414 GO:0023052 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033612 GO:0033993 GO:0034293 GO:0036211 GO:0038023 GO:0042175 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046777 GO:0048229 GO:0048236 GO:0048316 GO:0048367 GO:0048437 GO:0048545 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0098827 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

329

Amino Acids

35.5

Weight (kDa)

8.97

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB1I GGYRCC 4 cut(s) 410, 710, 792, 965
AccB7I CCANNNNNTGG 3 cut(s) 272, 557, 623
AccI GTMKAC 1 cut(s) 956
AciI CCGC 4 cut(s) 467, 683, 741, 806
AclWI GGATC 6 cut(s) 162, 381, 525, 612, 649, 841
AcsI RAATTY 3 cut(s) 19, 286, 812
AcuI CTGAAG 1 cut(s) 869
AcyI GRCGYC 2 cut(s) 793, 966
AfaI GTAC 4 cut(s) 181, 513, 828, 854
AfiI CCNNNNNNNGG 4 cut(s) 272, 557, 623, 638
AgsI TTSAA 3 cut(s) 385, 509, 693
AjnI CCWGG 4 cut(s) 406, 637, 697, 829
AjuI GAANNNNNNNTTGG 2 cut(s) 842, 874
AluBI AGCT 7 cut(s) 271, 304, 360, 369, 424, 462, 867
AluI AGCT 7 cut(s) 271, 304, 360, 369, 424, 462, 867
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 539
AlwI GGATC 6 cut(s) 162, 381, 525, 612, 649, 841
AlwNI CAGNNNCTG 1 cut(s) 623
AoxI GGCC 2 cut(s) 274, 613
ApeKI GCWGC 4 cut(s) 242, 749, 764, 864
ApoI RAATTY 3 cut(s) 19, 286, 812
AspLEI GCGC 2 cut(s) 795, 968
AspS9I GGNCC 5 cut(s) 114, 127, 326, 470, 614
AsuHPI GGTGA 3 cut(s) 104, 376, 648
AvaII GGWCC 4 cut(s) 114, 127, 326, 470
BanI GGYRCC 4 cut(s) 410, 710, 792, 965
BanII GRGCYC 1 cut(s) 306
BauI CACGAG 1 cut(s) 841
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 4 cut(s) 254, 736, 751, 876
BccI CCATC 1 cut(s) 970
BcgI CGANNNNNNTGC 1 cut(s) 962
BciT130I CCWGG 4 cut(s) 408, 639, 699, 831
BcoDI GTCTC 1 cut(s) 539
BfaI CTAG 2 cut(s) 336, 985
BfmI CTRYAG 1 cut(s) 307
BfoI RGCGCY 2 cut(s) 796, 969
BglI GCCNNNNNGGC 1 cut(s) 719
BisI GCNGC 6 cut(s) 243, 741, 750, 765, 806, 865
BlsI GCNGC 6 cut(s) 244, 742, 751, 766, 807, 866
BmcAI AGTACT 1 cut(s) 513
Bme1390I CCNGG 4 cut(s) 408, 639, 699, 831
Bme18I GGWCC 4 cut(s) 114, 127, 326, 470
BmgT120I GGNCC 5 cut(s) 114, 127, 326, 470, 614
BmiI GGNNCC 8 cut(s) 129, 350, 412, 471, 562, 712, 794, 967
BmrFI CCNGG 4 cut(s) 408, 639, 699, 831
BmrI ACTGGG 3 cut(s) 353, 611, 726
BmsI GCATC 1 cut(s) 85
BmuI ACTGGG 3 cut(s) 353, 611, 726
BpuEI CTTGAG 2 cut(s) 266, 853
Bsa29I ATCGAT 2 cut(s) 157, 836
BsaAI YACGTR 1 cut(s) 183
BsaHI GRCGYC 2 cut(s) 793, 966
BsaJI CCNNGG 3 cut(s) 406, 414, 637
BsaXI ACNNNNNCTCC 2 cut(s) 545, 575
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 557, 623, 638
Bse1I ACTGG 5 cut(s) 348, 519, 617, 628, 721
BseBI CCWGG 4 cut(s) 408, 639, 699, 831
BseCI ATCGAT 2 cut(s) 157, 836
BseDI CCNNGG 3 cut(s) 406, 414, 637
BseGI GGATG 2 cut(s) 52, 69
BseLI CCNNNNNNNGG 4 cut(s) 272, 557, 623, 638
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 5 cut(s) 348, 519, 617, 628, 721
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 4 cut(s) 254, 736, 751, 876
BshFI GGCC 2 cut(s) 276, 615
BshNI GGYRCC 4 cut(s) 410, 710, 792, 965
BshVI ATCGAT 2 cut(s) 157, 836
BsiHKAI GWGCWC 1 cut(s) 306
BsiSI CCGG 2 cut(s) 445, 796
BslI CCNNNNNNNGG 4 cut(s) 272, 557, 623, 638
BsmAI GTCTC 1 cut(s) 539
BsmBI CGTCTC 1 cut(s) 539
BsnI GGCC 2 cut(s) 276, 615
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
BspACI CCGC 4 cut(s) 467, 683, 741, 806
BspANI GGCC 2 cut(s) 276, 615
BspCNI CTCAG 1 cut(s) 551
BspDI ATCGAT 2 cut(s) 157, 836
BspLI GGNNCC 8 cut(s) 129, 350, 412, 471, 562, 712, 794, 967
BspPI GGATC 6 cut(s) 162, 381, 525, 612, 649, 841
BspT107I GGYRCC 4 cut(s) 410, 710, 792, 965
BsrI ACTGG 5 cut(s) 348, 519, 617, 628, 721
BssECI CCNNGG 3 cut(s) 406, 414, 637
BssMI GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
BssNI GRCGYC 2 cut(s) 793, 966
BssSI CACGAG 1 cut(s) 841
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 841
Bst2UI CCWGG 4 cut(s) 408, 639, 699, 831
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 505
BstACI GRCGYC 2 cut(s) 793, 966
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 78, 422
BstDEI CTNAG 2 cut(s) 110, 538
BstF5I GGATG 2 cut(s) 52, 69
BstH2I RGCGCY 2 cut(s) 796, 969
BstHHI GCGC 2 cut(s) 795, 968
BstKTI GATC 9 cut(s) 157, 161, 232, 376, 520, 607, 644, 836, 840
BstMAI GTCTC 1 cut(s) 539
BstMBI GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
BstMWI GCNNNNNNNGC 6 cut(s) 366, 417, 719, 746, 749, 770
BstNI CCWGG 4 cut(s) 408, 639, 699, 831
BstNSI RCATGY 1 cut(s) 201
BstSCI CCNGG 4 cut(s) 406, 637, 697, 829
BstSFI CTRYAG 1 cut(s) 307
BstV1I GCAGC 4 cut(s) 254, 736, 751, 876
BstX2I RGATCY 4 cut(s) 373, 517, 604, 641
BstYI RGATCY 4 cut(s) 373, 517, 604, 641
Bsu15I ATCGAT 2 cut(s) 157, 836
BsuRI GGCC 2 cut(s) 276, 615
BsuTUI ATCGAT 2 cut(s) 157, 836
BtsCI GGATG 2 cut(s) 52, 69
BtsIMutI CAGTG 1 cut(s) 714
Cac8I GCNNGC 2 cut(s) 78, 422
CaiI CAGNNNCTG 1 cut(s) 623
CfoI GCGC 2 cut(s) 795, 968
Cfr13I GGNCC 5 cut(s) 114, 127, 326, 470, 614
ClaI ATCGAT 2 cut(s) 157, 836
CseI GACGC 1 cut(s) 205
Csp6I GTAC 4 cut(s) 180, 512, 827, 853
CviAII CATG 6 cut(s) 191, 198, 319, 481, 601, 786
CviQI GTAC 4 cut(s) 180, 512, 827, 853
DdeI CTNAG 2 cut(s) 110, 538
DinI GGCGCC 2 cut(s) 794, 967
DpnI GATC 9 cut(s) 156, 160, 231, 375, 519, 606, 643, 835, 839
DpnII GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 505
EarI CTCTTC 1 cut(s) 505
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 414
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 4 cut(s) 114, 127, 326, 470
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 4 cut(s) 406, 637, 697, 829
EcoT14I CCWWGG 1 cut(s) 414
EcoT38I GRGCYC 1 cut(s) 306
EgeI GGCGCC 2 cut(s) 794, 967
EheI GGCGCC 2 cut(s) 794, 967
ErhI CCWWGG 1 cut(s) 414
Esp3I CGTCTC 1 cut(s) 539
FaeI CATG 6 cut(s) 194, 201, 322, 484, 604, 789
FalI AAGNNNNNCTT 2 cut(s) 842, 874
FatI CATG 6 cut(s) 190, 197, 318, 480, 600, 785
FauI CCCGC 1 cut(s) 460
FblI GTMKAC 1 cut(s) 956
Fnu4HI GCNGC 6 cut(s) 243, 741, 750, 765, 806, 865
FokI GGATG 2 cut(s) 39, 76
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 6 cut(s) 243, 741, 750, 765, 806, 865
FspBI CTAG 2 cut(s) 336, 985
GlaI GCGC 2 cut(s) 794, 967
GluI GCNGC 6 cut(s) 243, 741, 750, 765, 806, 865
HaeII RGCGCY 2 cut(s) 796, 969
HaeIII GGCC 2 cut(s) 276, 615
HapII CCGG 2 cut(s) 445, 796
HgaI GACGC 1 cut(s) 205
HhaI GCGC 2 cut(s) 795, 968
Hin1I GRCGYC 2 cut(s) 793, 966
Hin1II CATG 6 cut(s) 194, 201, 322, 484, 604, 789
Hin6I GCGC 2 cut(s) 793, 966
HinP1I GCGC 2 cut(s) 793, 966
HincII GTYRAC 1 cut(s) 257
HindII GTYRAC 1 cut(s) 257
HinfI GANTC 2 cut(s) 438, 475
HpaII CCGG 2 cut(s) 445, 796
HphI GGTGA 3 cut(s) 104, 376, 648
Hpy166II GTNNAC 5 cut(s) 172, 257, 326, 341, 957
Hpy188I TCNGA 1 cut(s) 474
Hpy188III TCNNGA 5 cut(s) 283, 540, 551, 841, 870
Hpy8I GTNNAC 5 cut(s) 172, 257, 326, 341, 957
HpyAV CCTTC 2 cut(s) 83, 287
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 9 cut(s) 103, 145, 201, 242, 773, 779, 785, 901, 973
HpyF10VI GCNNNNNNNGC 6 cut(s) 366, 417, 719, 746, 749, 770
HpyF3I CTNAG 2 cut(s) 110, 538
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 2 cut(s) 793, 966
Hsp92II CATG 6 cut(s) 194, 201, 322, 484, 604, 789
HspAI GCGC 2 cut(s) 793, 966
KasI GGCGCC 2 cut(s) 792, 965
Kzo9I GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
LmnI GCTCC 2 cut(s) 301, 566
Lsp1109I GCAGC 4 cut(s) 254, 736, 751, 876
LweI GCATC 1 cut(s) 85
MaeI CTAG 2 cut(s) 336, 985
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 193, 619
MalI GATC 9 cut(s) 156, 160, 231, 375, 519, 606, 643, 835, 839
MboI GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
MboII GAAGA 1 cut(s) 492
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 4 cut(s) 373, 517, 604, 641
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 7 cut(s) 19, 286, 330, 687, 774, 812, 889
Mly113I GGCGCC 2 cut(s) 793, 966
MnlI CCTC 8 cut(s) 118, 452, 657, 669, 695, 792, 866, 931
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 2 cut(s) 44, 784
MspA1I CMGCKG 1 cut(s) 743
MspI CCGG 2 cut(s) 445, 796
MspR9I CCNGG 4 cut(s) 408, 639, 699, 831
MunI CAATTG 1 cut(s) 774
MvaI CCWGG 4 cut(s) 408, 639, 699, 831
MwoI GCNNNNNNNGC 6 cut(s) 366, 417, 719, 746, 749, 770
NarI GGCGCC 2 cut(s) 793, 966
NdeII GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
NlaIII CATG 6 cut(s) 194, 201, 322, 484, 604, 789
NlaIV GGNNCC 8 cut(s) 129, 350, 412, 471, 562, 712, 794, 967
NspI RCATGY 1 cut(s) 201
PfeI GAWTC 2 cut(s) 438, 475
PflMI CCANNNNNTGG 3 cut(s) 272, 557, 623
PkrI GCNGC 6 cut(s) 244, 742, 751, 766, 807, 866
PluTI GGCGCC 2 cut(s) 796, 969
Ppu21I YACGTR 1 cut(s) 183
PpuMI RGGWCCY 1 cut(s) 127
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 4 cut(s) 406, 637, 697, 829
PspGI CCWGG 4 cut(s) 406, 637, 697, 829
PspN4I GGNNCC 8 cut(s) 129, 350, 412, 471, 562, 712, 794, 967
PspPI GGNCC 5 cut(s) 114, 127, 326, 470, 614
PspPPI RGGWCCY 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 4 cut(s) 373, 517, 604, 641
RsaI GTAC 4 cut(s) 181, 513, 828, 854
RsaNI GTAC 4 cut(s) 180, 512, 827, 853
RseI CAYNNNNRTG 2 cut(s) 44, 784
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 6 cut(s) 243, 741, 750, 765, 806, 865
Sau3AI GATC 9 cut(s) 154, 158, 229, 373, 517, 604, 641, 833, 837
Sau96I GGNCC 5 cut(s) 114, 127, 326, 470, 614
ScaI AGTACT 1 cut(s) 513
ScrFI CCNGG 4 cut(s) 408, 639, 699, 831
SduI GDGCHC 1 cut(s) 306
SfaNI GCATC 1 cut(s) 85
SfcI CTRYAG 1 cut(s) 307
SfoI GGCGCC 2 cut(s) 794, 967
SinI GGWCC 4 cut(s) 114, 127, 326, 470
SmiMI CAYNNNNRTG 2 cut(s) 44, 784
SmlI CTYRAG 2 cut(s) 281, 868
SmoI CTYRAG 2 cut(s) 281, 868
Sse9I AATT 7 cut(s) 19, 286, 330, 687, 774, 812, 889
SsiI CCGC 4 cut(s) 467, 683, 741, 806
SspDI GGCGCC 2 cut(s) 792, 965
SspI AATATT 1 cut(s) 296
SspMI CTAG 2 cut(s) 336, 985
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 4 cut(s) 406, 637, 697, 829
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 185
TaqI TCGA 2 cut(s) 157, 836
TasI AATT 7 cut(s) 19, 286, 330, 687, 774, 812, 889
TatI WGTACW 2 cut(s) 511, 852
TauI GCSGC 2 cut(s) 743, 808
TfiI GAWTC 2 cut(s) 438, 475
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 1 cut(s) 721
TseI GCWGC 4 cut(s) 242, 749, 764, 864
TspDTI ATGAA 2 cut(s) 28, 179
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 1 cut(s) 721
Van91I CCANNNNNTGG 3 cut(s) 272, 557, 623
VpaK11BI GGWCC 4 cut(s) 114, 127, 326, 470
XapI RAATTY 3 cut(s) 19, 286, 812
XceI RCATGY 1 cut(s) 201
XmiI GTMKAC 1 cut(s) 956
XspI CTAG 2 cut(s) 336, 985
ZrmI AGTACT 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.