pycom05g06410

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005580
Physical Location & Seq
Forward (+)
8696867 .. 8702935
6069 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2736 bp
ATGGCTGCTGTGATGAGAAAACTGAGTTCTGCTATGGCGGCTTCTAGTGGCAGCGACAGGGATGGTGGGGATTTTAGCATGGAGTTGGAGTTTTCGTTTGCCGTGGAGTACAAGGGTCCTCCGGTGGGTTATGATATCCCAAATGCATTTCCTGTTGACATTCGCCACATCCCAACTGCTGCTCCGGTTTCTTCAGCCTCTCTCTTGAACAATTTGTCCCTCCCTGTTATTCATCCGATTGCGAAATCCACCAAGAAACCAAAAGAAGAGACGAAACTTGAAACTGAAGTAAAGTTTGGAAAGGAAATTGTTTCTTCCAATTCAGTAACCCCTGCTGAATTGGAAATTAGCAATGGCGAAAAGGGTGTCTCGACTTTATCAGATAAAGGTGAAATCTCTGGGAAATTGGGGTCTTCTGGCAGTCTGTGTGGAAGTTTGGAGGGTGTGCTTGAATTGCCTGATGATGGTCAAGAAGCTCAAGGCTTTCAGAATTATATGAGCCCCGGTAACTGGGGTTCATCAGAGTCTGGTTCGAGTTTGCGGAGTCTTTCCTCTGAGGTTTTCTCTGGTAGAGAGGAAGCTTATACGGATGCAGTTCCTCACCATGTCAAAAGGCCATCAGCTGTGACATTCCGTGATCCTGACTCGAATGACATTGTTCAGGAAGAAGAGTTTGATCGATCACGTCCTTCTGAAGGGGAAGAAGATGTGCGAGTGAGGCCAACGATTGAGAGAAGTGGGAAGAAAGGGTCGTGCTATCGATGTGGGAAAGGAAACAGACTCATGGAGAAGGAGGTTTGCATTGTTTGTGGTGCTAAGTTTTGCTATAATTGTGTGCTAAGAGCAATGGGATCAATGCCGGAAGGAAGGAAATGTGTTACTTGCATTAGTTTTGAAATTGACGAGTCAAGGCGAAGGAAACTGGGCAAGTGTTCTAGGATGCTGAAGCGGCTTCTCACTAAATCGGAAGTTGAACTGATAATGAAGGCCGAGATATCTTGCCAAGCAAATCAACTGCCAGGCAATCTTATTTTTGTGAATGGCGAACCTTTATCGCAGGAAGAGCTGGTTCGATTGCAAAGTTGCCGAAACCCACCAAAAAAGATAAAACCAGGATGCTATTGGTACGATAATGTATCTGGTTTTTGGGGAAAGGAAGGGCATAGGCCTTGCCAAATCATTAGCCCCCGGTTGAGTGTGGGAGGCCACATACAACGAAATGCTAGTAACGGGGATACAAATATATTGATTAACAATCGGGAGATCACTAAGTTAGAGGCGTTCATGCTGAAGTTGGCTGGAGTGCCATGTGAAGGAAATCTTCACTACTGGGTGAGTGCAGAGGGGGAGTATCAGGAAGAGGGAATGAATCAAGTAAAGGGAAAGATATGGGACAAGGCTACAATAAAGATGGTTTGTAACATCTTGTCTTTGCCATTTCCTTCTGGTTCACCAAATCTTTCCTCTGACGAAGTGAACACCTGGAACACCTTTGAAGAGAAAAAGCTTAACAAAATTGTTCTAGTTGGACATCACAAATCTGGTACAAGCACTATATTTAAACAGGCCAAGTTCTTGTACAATGTTCCTTTCTCTGAAGATGAGCGCCAAAGTATTAAGTTCGTGATCCAGAGCAGATTGTACAGTTATCTTGGTATATTGCTTGAAGGGCGTGAGTGGTTTGAAGAAGAAAGTTTGATTGAGAAGAGAAAAGGAAAAGGACGGCTTTTGGATGAACCTGGCCCTTCAGGGAATACAAGCCAGCTTGACGATAAAACAAAATATTCCATTGGTCCAAGACTGAAAGCTTTTTCTGATTGGCTCCTCAAATCTATGGTATCTGGTAATCTGGAAGTCATATTTCCAGCAGCTACCCGTGAATATGCACCATTTGTTGAGGATTTGTGGAAGGATCCTGCTATTCAAGCCACATACGACCGGAGGAATGAAATAGAAATGCTGCCCAGACCAGCTGCATATTTTCTTAATCGGGCTGTTGAGATTTCAAGGACAGATTATGAGCCCACAGACACGGACATCTTGTATGCCGAGGGAATCACCTCATCCAACAGCCTTATGAGCATGGAATTCTCAATTCCCCCATCAGCTGGAAATAGCAATCTGGACCCTCCTTATCAGCATGACCCTTCGTTAAGGTACCAACTAATTAGAGTGCATCAGAGCACCCTCGGAGGAAACTGCAAGTTGGTTGAGATGTTTGAAGATGTTGATATGGTCGCATTTTGTGTTTCATTGACAGAATACGATGAGTTTTCTACAGATGCTAACGGGGTTTCCATAAACAAGATGATGGCAAGCAAGCAGCTATTTGAAAATATTGTTACCCATCCAGCCTTTGACCAAAAAGGCTTCCTTCTGATACTCAACAAGTATGATCTGCTTGAAGAAAAGATTGACGAAGCCCCTCTCACTCGATGTGAATGGTTTCATGACTTCAATCCGGTAACCAGCCAAAATCCCAGTGGTAGCAATAACAACAGTAATAACCCCACGTTGGCTCACCGAGCCTTTCAATATATTGCAGTGAAGTTCAAAAGACTCTTTCATTCTCTAACAGATAGGAAGCTGTTTGTTTCACTGGTTACAGCGCTAGAGTCTGATACTGTAAATGAAGCTCTTAGATATGCTAGTGAGATTCTAAAATGGGAGGAAGAGGTACCCAGATTAATTCACGAGATCTCTTCCGCTAGCATTGAGGCAAGTTCTTCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

912

Amino Acids

101.64

Weight (kDa)

5.55

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 500 - 885 1.9e-64 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 2157, 2677
AccB1I GGYRCC 2 cut(s) 2157, 2677
AccB7I CCANNNNNTGG 1 cut(s) 2108
AciI CCGC 5 cut(s) 38, 541, 949, 2706, 2730
AclWI GGATC 5 cut(s) 632, 859, 1621, 1907, 1920
AcsI RAATTY 1 cut(s) 2087
AcuI CTGAAG 7 cut(s) 177, 306, 714, 965, 1310, 1617, 1731
AfaI GTAC 7 cut(s) 110, 1127, 1546, 1580, 1643, 2159, 2679
AfeI AGCGCT 1 cut(s) 2610
AfiI CCNNNNNNNGG 7 cut(s) 125, 464, 510, 695, 1313, 2108, 2515
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 4 cut(s) 1018, 1111, 1481, 1738
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
Alw21I GWGCWC 1 cut(s) 2186
Alw26I GTCTC 2 cut(s) 263, 373
AlwI GGATC 5 cut(s) 632, 859, 1621, 1907, 1920
AlwNI CAGNNNCTG 1 cut(s) 527
Aor51HI AGCGCT 1 cut(s) 2610
AoxI GGCC 7 cut(s) 614, 719, 987, 1166, 1204, 1566, 1741
ApeKI GCWGC 7 cut(s) 5, 51, 179, 1868, 1960, 1973, 2323
ApoI RAATTY 1 cut(s) 2087
ArsI GACNNNNNNTTYG 2 cut(s) 351, 383
AseI ATTAAT 1 cut(s) 2687
Asp700I GAANNNNTTC 1 cut(s) 2445
Asp718I GGTACC 2 cut(s) 2157, 2677
AspLEI GCGC 2 cut(s) 1608, 2611
AspS9I GGNCC 4 cut(s) 116, 1742, 1793, 2125
AsuC2I CCSGG 2 cut(s) 504, 1189
AsuHPI GGTGA 6 cut(s) 401, 593, 1345, 1443, 2050, 2513
AsuNHI GCTAGC 1 cut(s) 2708
AvaII GGWCC 3 cut(s) 116, 1793, 2125
BamHI GGATCC 1 cut(s) 1912
BanI GGYRCC 2 cut(s) 2157, 2677
BanII GRGCYC 2 cut(s) 503, 2025
BauI CACGAG 1 cut(s) 2693
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 2186
BbvI GCAGC 6 cut(s) 63, 166, 1880, 1947, 1960, 2335
BccI CCATC 7 cut(s) 56, 458, 625, 1405, 2110, 2305, 2355
BceAI ACGGC 2 cut(s) 86, 1739
BciT130I CCWGG 4 cut(s) 1020, 1113, 1483, 1740
BciVI GTATCC 1 cut(s) 1228
BcnI CCSGG 2 cut(s) 504, 1189
BcoDI GTCTC 2 cut(s) 263, 373
BfaI CTAG 7 cut(s) 45, 936, 1224, 1523, 2612, 2649, 2709
BfmI CTRYAG 1 cut(s) 2277
BfoI RGCGCY 2 cut(s) 1609, 2612
BfuI GTATCC 1 cut(s) 1228
BglII AGATCT 1 cut(s) 2697
BisI GCNGC 9 cut(s) 6, 39, 52, 180, 950, 1869, 1961, 1974, 2324
BlsI GCNGC 9 cut(s) 7, 40, 53, 181, 951, 1870, 1962, 1975, 2325
Bme1390I CCNGG 6 cut(s) 504, 1020, 1113, 1189, 1483, 1740
Bme18I GGWCC 3 cut(s) 116, 1793, 2125
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 4 cut(s) 116, 1742, 1793, 2125
BmiI GGNNCC 6 cut(s) 117, 1823, 1914, 2127, 2159, 2679
BmrFI CCNGG 6 cut(s) 504, 1020, 1113, 1189, 1483, 1740
BmrI ACTGGG 4 cut(s) 520, 932, 1339, 2475
BmsI GCATC 5 cut(s) 580, 930, 1106, 2185, 2272
BmtI GCTAGC 1 cut(s) 2712
BmuI ACTGGG 4 cut(s) 520, 932, 1339, 2475
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 1 cut(s) 1320
BpuEI CTTGAG 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 504, 1189
Bsa29I ATCGAT 2 cut(s) 679, 760
BsaJI CCNNGG 5 cut(s) 102, 502, 1187, 2049, 2188
BsaWI WCCGGW 4 cut(s) 121, 184, 1938, 2461
BsaXI ACNNNNNCTCC 6 cut(s) 74, 80, 104, 110, 166, 196
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 464, 510, 695, 1313, 2108, 2515
Bse1I ACTGG 5 cut(s) 515, 927, 1334, 2481, 2604
Bse3DI GCAATG 2 cut(s) 358, 852
BseBI CCWGG 4 cut(s) 1020, 1113, 1483, 1740
BseCI ATCGAT 2 cut(s) 679, 760
BseDI CCNNGG 5 cut(s) 102, 502, 1187, 2049, 2188
BseGI GGATG 9 cut(s) 67, 168, 232, 595, 945, 1121, 1738, 2063, 2347
BseLI CCNNNNNNNGG 7 cut(s) 125, 464, 510, 695, 1313, 2108, 2515
BseMI GCAATG 2 cut(s) 358, 852
BseMII CTCAG 2 cut(s) 14, 546
BseNI ACTGG 5 cut(s) 515, 927, 1334, 2481, 2604
BseRI GAGGAG 1 cut(s) 1814
BseXI GCAGC 6 cut(s) 63, 166, 1880, 1947, 1960, 2335
BsgI GTGCAG 1 cut(s) 1359
Bsh1285I CGRYCG 1 cut(s) 1939
BshFI GGCC 7 cut(s) 616, 721, 989, 1168, 1206, 1568, 1743
BshNI GGYRCC 2 cut(s) 2157, 2677
BshVI ATCGAT 2 cut(s) 679, 760
BsiEI CGRYCG 1 cut(s) 1939
BsiHKAI GWGCWC 1 cut(s) 2186
BsiSI CCGG 7 cut(s) 122, 185, 504, 860, 1189, 1939, 2462
BslFI GGGAC 2 cut(s) 202, 1406
BslI CCNNNNNNNGG 7 cut(s) 125, 464, 510, 695, 1313, 2108, 2515
BsmAI GTCTC 2 cut(s) 263, 373
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 2 cut(s) 202, 1406
BsnI GGCC 7 cut(s) 616, 721, 989, 1168, 1206, 1568, 1743
Bsp1286I GDGCHC 3 cut(s) 503, 2025, 2186
Bsp1407I TGTACA 2 cut(s) 1578, 1641
Bsp143I GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
BspACI CCGC 5 cut(s) 38, 541, 949, 2706, 2730
BspANI GGCC 7 cut(s) 616, 721, 989, 1168, 1206, 1568, 1743
BspCNI CTCAG 2 cut(s) 15, 547
BspDI ATCGAT 2 cut(s) 679, 760
BspHI TCATGA 1 cut(s) 2449
BspLI GGNNCC 6 cut(s) 117, 1823, 1914, 2127, 2159, 2679
BspOI GCTAGC 1 cut(s) 2712
BspPI GGATC 5 cut(s) 632, 859, 1621, 1907, 1920
BspQI GCTCTTC 1 cut(s) 1056
BspT107I GGYRCC 2 cut(s) 2157, 2677
BsrDI GCAATG 2 cut(s) 358, 852
BsrGI TGTACA 2 cut(s) 1578, 1641
BsrI ACTGG 5 cut(s) 515, 927, 1334, 2481, 2604
BssECI CCNNGG 5 cut(s) 102, 502, 1187, 2049, 2188
BssMI GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
BssSI CACGAG 1 cut(s) 2693
Bst2BI CACGAG 1 cut(s) 2693
Bst2UI CCWGG 4 cut(s) 1020, 1113, 1483, 1740
Bst4CI ACNGT 3 cut(s) 1646, 2501, 2626
Bst6I CTCTTC 8 cut(s) 261, 663, 1056, 1353, 1491, 1700, 2667, 2707
BstAUI TGTACA 2 cut(s) 1578, 1641
BstC8I GCNNGC 4 cut(s) 1763, 2317, 2321, 2710
BstDEI CTNAG 6 cut(s) 23, 555, 816, 839, 1269, 2639
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 2464
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 9 cut(s) 67, 168, 232, 595, 945, 1121, 1738, 2063, 2347
BstH2I RGCGCY 2 cut(s) 1609, 2612
BstHHI GCGC 2 cut(s) 1608, 2611
BstKTI GATC 9 cut(s) 640, 679, 683, 854, 1266, 1629, 1915, 2398, 2700
BstMAI GTCTC 2 cut(s) 263, 373
BstMBI GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
BstMCI CGRYCG 1 cut(s) 1939
BstMWI GCNNNNNNNGC 9 cut(s) 38, 454, 718, 949, 1063, 1669, 1925, 2079, 2525
BstNI CCWGG 4 cut(s) 1020, 1113, 1483, 1740
BstPI GGTNACC 1 cut(s) 2464
BstSCI CCNGG 6 cut(s) 502, 1018, 1111, 1187, 1481, 1738
BstSFI CTRYAG 1 cut(s) 2277
BstV1I GCAGC 6 cut(s) 63, 166, 1880, 1947, 1960, 2335
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 2 cut(s) 1912, 2697
BstYI RGATCY 2 cut(s) 1912, 2697
Bsu15I ATCGAT 2 cut(s) 679, 760
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 7 cut(s) 616, 721, 989, 1168, 1206, 1568, 1743
BsuTUI ATCGAT 2 cut(s) 679, 760
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 9 cut(s) 67, 168, 232, 595, 945, 1121, 1738, 2063, 2347
BtsI GCAGTG 1 cut(s) 2550
BtsIMutI CAGTG 3 cut(s) 2488, 2550, 2597
Cac8I GCNNGC 4 cut(s) 1763, 2317, 2321, 2710
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 1 cut(s) 2449
CfoI GCGC 2 cut(s) 1608, 2611
Cfr13I GGNCC 4 cut(s) 116, 1742, 1793, 2125
ClaI ATCGAT 2 cut(s) 679, 760
Csp6I GTAC 7 cut(s) 109, 1126, 1545, 1579, 1642, 2158, 2678
CviAII CATG 8 cut(s) 79, 605, 784, 1285, 1308, 2083, 2141, 2450
CviQI GTAC 7 cut(s) 109, 1126, 1545, 1579, 1642, 2158, 2678
DdeI CTNAG 6 cut(s) 23, 555, 816, 839, 1269, 2639
DpnI GATC 9 cut(s) 639, 678, 682, 853, 1265, 1628, 1914, 2397, 2699
DpnII GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
DraI TTTAAA 1 cut(s) 1561
Eam1104I CTCTTC 8 cut(s) 261, 663, 1056, 1353, 1491, 1700, 2667, 2707
EarI CTCTTC 8 cut(s) 261, 663, 1056, 1353, 1491, 1700, 2667, 2707
Eco147I AGGCCT 1 cut(s) 1168
Eco24I GRGCYC 2 cut(s) 503, 2025
Eco32I GATATC 2 cut(s) 136, 996
Eco47I GGWCC 3 cut(s) 116, 1793, 2125
Eco47III AGCGCT 1 cut(s) 2610
Eco57I CTGAAG 7 cut(s) 177, 306, 714, 965, 1310, 1617, 1731
Eco91I GGTNACC 1 cut(s) 2464
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoO109I RGGNCCY 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 2464
EcoRI GAATTC 1 cut(s) 2087
EcoRII CCWGG 4 cut(s) 1018, 1111, 1481, 1738
EcoRV GATATC 2 cut(s) 136, 996
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 503, 2025
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 8 cut(s) 82, 608, 787, 1288, 1311, 2086, 2144, 2453
FalI AAGNNNNNCTT 6 cut(s) 1305, 1337, 1710, 1742, 2358, 2390
FaqI GGGAC 2 cut(s) 202, 1406
FatI CATG 8 cut(s) 78, 604, 783, 1284, 1307, 2082, 2140, 2449
Fnu4HI GCNGC 9 cut(s) 6, 39, 52, 180, 950, 1869, 1961, 1974, 2324
FokI GGATG 9 cut(s) 74, 155, 219, 602, 952, 1128, 1745, 2050, 2334
FriOI GRGCYC 2 cut(s) 503, 2025
Fsp4HI GCNGC 9 cut(s) 6, 39, 52, 180, 950, 1869, 1961, 1974, 2324
FspBI CTAG 7 cut(s) 45, 936, 1224, 1523, 2612, 2649, 2709
GlaI GCGC 2 cut(s) 1607, 2610
GluI GCNGC 9 cut(s) 6, 39, 52, 180, 950, 1869, 1961, 1974, 2324
GsuI CTGGAG 1 cut(s) 1320
HaeII RGCGCY 2 cut(s) 1609, 2612
HaeIII GGCC 7 cut(s) 616, 721, 989, 1168, 1206, 1568, 1743
HapII CCGG 7 cut(s) 122, 185, 504, 860, 1189, 1939, 2462
HhaI GCGC 2 cut(s) 1608, 2611
Hin1II CATG 8 cut(s) 82, 608, 787, 1288, 1311, 2086, 2144, 2453
Hin6I GCGC 2 cut(s) 1606, 2609
HinP1I GCGC 2 cut(s) 1606, 2609
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HindIII AAGCTT 3 cut(s) 579, 1505, 1806
HpaII CCGG 7 cut(s) 122, 185, 504, 860, 1189, 1939, 2462
HphI GGTGA 6 cut(s) 401, 593, 1345, 1443, 2050, 2513
Hpy166II GTNNAC 3 cut(s) 157, 1451, 1477
Hpy8I GTNNAC 3 cut(s) 157, 1451, 1477
HpyCH4III ACNGT 3 cut(s) 1646, 2501, 2626
HpyCH4IV ACGT 2 cut(s) 685, 2513
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 454, 718, 949, 1063, 1669, 1925, 2079, 2525
HpyF3I CTNAG 6 cut(s) 23, 555, 816, 839, 1269, 2639
HpySE526I ACGT 2 cut(s) 685, 2513
Hsp92II CATG 8 cut(s) 82, 608, 787, 1288, 1311, 2086, 2144, 2453
HspAI GCGC 2 cut(s) 1606, 2609
KpnI GGTACC 2 cut(s) 2161, 2681
Kzo9I GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
LguI GCTCTTC 1 cut(s) 1056
LmnI GCTCC 2 cut(s) 187, 1827
Lsp1109I GCAGC 6 cut(s) 63, 166, 1880, 1947, 1960, 2335
LweI GCATC 5 cut(s) 580, 930, 1106, 2185, 2272
MaeI CTAG 7 cut(s) 45, 936, 1224, 1523, 2612, 2649, 2709
MaeII ACGT 2 cut(s) 685, 2513
MaeIII GTNAC 9 cut(s) 325, 506, 625, 877, 1226, 1418, 2341, 2464, 2602
MalI GATC 9 cut(s) 639, 678, 682, 853, 1265, 1628, 1914, 2397, 2699
MboI GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
MflI RGATCY 2 cut(s) 1912, 2697
MhlI GDGCHC 3 cut(s) 503, 2025, 2186
MlyI GAGTC 7 cut(s) 533, 553, 638, 774, 914, 2553, 2624
MmeI TCCRAC 3 cut(s) 66, 1507, 2091
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 2445
MseI TTAA 8 cut(s) 1251, 1509, 1560, 1617, 1986, 2153, 2687, 2734
MspA1I CMGCKG 3 cut(s) 623, 1973, 2108
MspI CCGG 7 cut(s) 122, 185, 504, 860, 1189, 1939, 2462
MspR9I CCNGG 6 cut(s) 504, 1020, 1113, 1189, 1483, 1740
MvaI CCWGG 4 cut(s) 1020, 1113, 1483, 1740
MwoI GCNNNNNNNGC 9 cut(s) 38, 454, 718, 949, 1063, 1669, 1925, 2079, 2525
NciI CCSGG 2 cut(s) 504, 1189
NdeII GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
NheI GCTAGC 1 cut(s) 2708
NlaIII CATG 8 cut(s) 82, 608, 787, 1288, 1311, 2086, 2144, 2453
NlaIV GGNNCC 6 cut(s) 117, 1823, 1914, 2127, 2159, 2679
NmeAIII GCCGAG 2 cut(s) 1015, 2074
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 1 cut(s) 148
PagI TCATGA 1 cut(s) 2449
PceI AGGCCT 1 cut(s) 1168
PciSI GCTCTTC 1 cut(s) 1056
PdmI GAANNNNTTC 1 cut(s) 2445
PfeI GAWTC 3 cut(s) 1369, 2055, 2656
PflMI CCANNNNNTGG 1 cut(s) 2108
PkrI GCNGC 9 cut(s) 7, 40, 53, 181, 951, 1870, 1962, 1975, 2325
PleI GAGTC 7 cut(s) 532, 552, 638, 774, 913, 2553, 2623
PpsI GAGTC 7 cut(s) 532, 552, 638, 774, 913, 2553, 2623
PpuMI RGGWCCY 1 cut(s) 116
PshBI ATTAAT 1 cut(s) 2687
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 4 cut(s) 1018, 1111, 1481, 1738
PspEI GGTNACC 1 cut(s) 2464
PspGI CCWGG 4 cut(s) 1018, 1111, 1481, 1738
PspN4I GGNNCC 6 cut(s) 117, 1823, 1914, 2127, 2159, 2679
PspPI GGNCC 4 cut(s) 116, 1742, 1793, 2125
PspPPI RGGWCCY 1 cut(s) 116
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 2 cut(s) 1912, 2697
PvuII CAGCTG 3 cut(s) 623, 1973, 2108
RsaI GTAC 7 cut(s) 110, 1127, 1546, 1580, 1643, 2159, 2679
RsaNI GTAC 7 cut(s) 109, 1126, 1545, 1579, 1642, 2158, 2678
SapI GCTCTTC 1 cut(s) 1056
SaqAI TTAA 8 cut(s) 1251, 1509, 1560, 1617, 1986, 2153, 2687, 2734
SatI GCNGC 9 cut(s) 6, 39, 52, 180, 950, 1869, 1961, 1974, 2324
Sau3AI GATC 9 cut(s) 637, 676, 680, 851, 1263, 1626, 1912, 2395, 2697
Sau96I GGNCC 4 cut(s) 116, 1742, 1793, 2125
SchI GAGTC 7 cut(s) 533, 553, 638, 774, 914, 2553, 2624
ScrFI CCNGG 6 cut(s) 504, 1020, 1113, 1189, 1483, 1740
SduI GDGCHC 3 cut(s) 503, 2025, 2186
SfaNI GCATC 5 cut(s) 580, 930, 1106, 2185, 2272
SfcI CTRYAG 1 cut(s) 2277
SinI GGWCC 3 cut(s) 116, 1793, 2125
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SseBI AGGCCT 1 cut(s) 1168
SsiI CCGC 5 cut(s) 38, 541, 949, 2706, 2730
SspI AATATT 2 cut(s) 1784, 2338
SspMI CTAG 7 cut(s) 45, 936, 1224, 1523, 2612, 2649, 2709
StuI AGGCCT 1 cut(s) 1168
StyD4I CCNGG 6 cut(s) 502, 1018, 1111, 1187, 1481, 1738
TaaI ACNGT 3 cut(s) 1646, 2501, 2626
TaiI ACGT 2 cut(s) 688, 2516
TaqI TCGA 7 cut(s) 371, 533, 647, 679, 760, 1072, 2434
TatI WGTACW 3 cut(s) 108, 1578, 1641
TauI GCSGC 2 cut(s) 41, 952
TfiI GAWTC 3 cut(s) 1369, 2055, 2656
Tru1I TTAA 8 cut(s) 1251, 1509, 1560, 1617, 1986, 2153, 2687, 2734
Tru9I TTAA 8 cut(s) 1251, 1509, 1560, 1617, 1986, 2153, 2687, 2734
TscAI CASTG 3 cut(s) 2488, 2550, 2604
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 7 cut(s) 5, 51, 179, 1868, 1960, 1973, 2323
Tsp45I GTSAC 1 cut(s) 625
TspGWI ACGGA 3 cut(s) 602, 623, 2048
TspRI CASTG 3 cut(s) 2488, 2550, 2604
Van91I CCANNNNNTGG 1 cut(s) 2108
VpaK11BI GGWCC 3 cut(s) 116, 1793, 2125
VspI ATTAAT 1 cut(s) 2687
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 1 cut(s) 2087
XcmI CCANNNNNNNNNTGG 2 cut(s) 1119, 2480
XmnI GAANNNNTTC 1 cut(s) 2445
XspI CTAG 7 cut(s) 45, 936, 1224, 1523, 2612, 2649, 2709
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.