pycom05g08400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019785
Physical Location & Seq
Forward (+)
11020696 .. 11023339
2644 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08400.4

Sequence Viewer

Length: 1896 bp
ATGCGGAAGAGAGTTCTAACGTGGAAAACCCAGTGTTCTAAAAATCGGCCTAGGCGGAGGTGTGCCAATGCGATTAAGTTCGAATCGTTAAGAAAAATCGGGGAGCTGTTAGGCGCCCACCTAGGCGCTCTGAGGCGGCCTCCTAGGCGCTCTGAGGGTTTCCTGGGAAGAAATTCCAAACAGAAACCCAAAATCACAAACTCGAATTTAAACTTGAAATTAACATGCAGAGCATGGGTTAAGTTACCTGAGTTTCGAACGGGATTAATTCGAGTTCATGGGACCCCAAGTTCGACGGGTCCCTCACCGGAGCTCCACGCCAGAAGCTCAATTTTCAAAGGTTGTAGCTCGATTCCATTAGAGCAACTCTCACCAAGAACCCCTGCTTGGATTCTCCTACTTTCCATCACAGAATTTAACATGCAAAGGTACCATAGGGATCCCCAGGTTGCGCAAGTCTTGCATTTGGTGGTTTGGGGTCTTGATTTAATTGGAATTTGTGGTGATGGTGCTTGGAGCTCTCTTTGGCTATGGTGGTTTAGAAAGAAGGCAGAGAGTTCGAAAGGAGAGAGAGAGAGAGAGAGAGAAGAGAACCGGTTTTCAAAAACAAAAATGATGGTGATGTGTGAGAGAGTGGAAAGTGCACGAGAACCTAGGGTTTGTGTGTCAGAGAGAGAGAGAAAATATGTGAGAGAGATGAGACTGATTGATATGAAATGGGGAAGGATGCATGCTCTGACTGGTTTTGGTGCTCAAAAGTTGAAAGACACCAATAAAATCATAAATAAAAGAGAGAGGATTTCGGGGATGATACCAATGGGCGTGGTTTCATTAAGGGATAACTGTTTTACTTTTATTTTTAAAAAATTTGATATAATCATTCAAAATGTCTTAAGTTATATGACATATGTGCTTAATGTGTTTTTAAATTTTAGACTTGTTGGATACTCTTTTTGTACTTTATCATTCTTTTATTATCTTATCCATGAATTTCATATAAGTATAATTTTTTATGTGCTAGCTCGTCACCCGACTAGCGCCCAGATTTTTTTAGAACCTTGGGAAAACCTTACTGAAAACCTCATCAACATAACTCTTGAAGCGATGAAAATTAAGAATTTTGTAAATATACTTCACAACCTTAAAAACAAAAACATTTTCATTTTGCATATCCTTGCACATTTAATATTTTGTAATAGACTAGTAGTGTATGAATGTTTTTTTATTATGCTGTTATTTTCGAGTGTAGATATAATTTATGTGGCAATGTGTGGTATGTGCAAATTTAAGAAAAAAAAAATATTTTTCTTAACGGGTCAAGTCACTTTACCTCCATTTGCCAGACCTAATTATGGTCTAGTGGACAAAAATGACACGAACACGATCCATTTGCCAGACCTAATTATGGTCTATGTGAGACTAGCCCCACCCCTTCCCCTTAGAAAAAAAATCTTTTGTGAGTTTATTGGGGGTGAGCTCAGAAGGATTTCTAGAATAAATGAAATAACACGATCTGTTTTGAGAAGCTTAGGTTTGATTATCTCTAAAGGCAAATTTGAACTACAAAAAAAAATCTTTTGTGAGTTTATTGGGGGTGATAAAAAAAAAAAGTTGAAATGGGGTGAGAGAGGCTCGAACTCTCGACCTCAGGATAACTCAGAAGCTATGAGACCTACGCGCTACCAATTTCAACCCTGTTGTAAGTTAACCTTAACCTTCAAAGTTAATCACGTCACTCCTGACGTGATGTGTTTCGGGGACCGTCGAAACTTCCCGGTACCAAACGACCTCTACCTCCACACGCGTCCACGTCACCCCCACGAACACCTCCAACGTAAAATCACCGACATACCCCCAAATCAAATAGAGGCCCGCAGGGGCAAAGCCGGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

632

Amino Acids

73.68

Weight (kDa)

10.11

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019785)

Species Orthologous Gene IDs
malus_domestica MD07G1185500.v1.1
pyrus_communis pycom01g04080 pycom05g08400 pycom07g12130 pycom11g13980 pycom17g16240 pycom17g17130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 453
Acc65I GGTACC 2 cut(s) 429, 1777
AccB1I GGYRCC 3 cut(s) 113, 429, 1777
AccII CGCG 2 cut(s) 1678, 1804
AciI CCGC 4 cut(s) 4, 55, 136, 1873
AclWI GGATC 3 cut(s) 434, 447, 1378
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 431, 958, 1779
AfiI CCNNNNNNNGG 3 cut(s) 387, 1352, 1405
AflII CTTAAG 1 cut(s) 892
AflIII ACRYGT 1 cut(s) 1802
AgeI ACCGGT 1 cut(s) 594
AhlI ACTAGT 1 cut(s) 1201
AjiI CACGTC 3 cut(s) 1732, 1744, 1811
AjnI CCWGG 2 cut(s) 162, 444
AjuI GAANNNNNNNTTGG 2 cut(s) 370, 402
AloI GAACNNNNNNTCC 2 cut(s) 274, 306
AluBI AGCT 9 cut(s) 106, 313, 327, 348, 519, 1022, 1477, 1527, 1664
AluI AGCT 9 cut(s) 106, 313, 327, 348, 519, 1022, 1477, 1527, 1664
Alw21I GWGCWC 5 cut(s) 315, 521, 646, 754, 1479
Alw26I GTCTC 3 cut(s) 694, 1411, 1663
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 3 cut(s) 434, 447, 1378
AoxI GGCC 3 cut(s) 47, 137, 1869
ApaLI GTGCAC 1 cut(s) 642
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
AseI ATTAAT 1 cut(s) 266
AsiGI ACCGGT 1 cut(s) 594
Asp700I GAANNNNTTC 2 cut(s) 172, 1486
Asp718I GGTACC 2 cut(s) 429, 1777
AspA2I CCTAGG 4 cut(s) 50, 121, 143, 653
AspLEI GCGC 6 cut(s) 116, 128, 150, 454, 1040, 1680
AspS9I GGNCC 4 cut(s) 282, 299, 1759, 1870
AsuC2I CCSGG 1 cut(s) 1775
AsuII TTCGAA 3 cut(s) 81, 256, 560
AsuNHI GCTAGC 1 cut(s) 1018
AvaII GGWCC 3 cut(s) 282, 299, 1759
AvrII CCTAGG 4 cut(s) 50, 121, 143, 653
AxyI CCTNAGG 1 cut(s) 1647
BaeGI GKGCMC 1 cut(s) 646
BamHI GGATCC 1 cut(s) 439
BanI GGYRCC 3 cut(s) 113, 429, 1777
BanII GRGCYC 3 cut(s) 315, 521, 1479
BarI GAAGNNNNNNTAC 2 cut(s) 1116, 1148
BauI CACGAG 1 cut(s) 645
Bbv12I GWGCWC 5 cut(s) 315, 521, 646, 754, 1479
BccI CCATC 3 cut(s) 413, 500, 610
BcgI CGANNNNNNTGC 4 cut(s) 540, 574, 1372, 1406
BciT130I CCWGG 2 cut(s) 164, 446
BciVI GTATCC 1 cut(s) 938
BcnI CCSGG 1 cut(s) 1775
BcoDI GTCTC 3 cut(s) 694, 1411, 1663
BcuI ACTAGT 1 cut(s) 1201
BfoI RGCGCY 4 cut(s) 117, 129, 151, 1041
BfrI CTTAAG 1 cut(s) 892
BfuI GTATCC 1 cut(s) 938
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 1 cut(s) 137
BlnI CCTAGG 4 cut(s) 50, 121, 143, 653
BlsI GCNGC 1 cut(s) 138
Bme1390I CCNGG 3 cut(s) 164, 446, 1775
Bme18I GGWCC 3 cut(s) 282, 299, 1759
BmgBI CACGTC 3 cut(s) 1732, 1744, 1811
BmgT120I GGNCC 4 cut(s) 282, 299, 1759, 1870
BmiI GGNNCC 9 cut(s) 115, 283, 284, 300, 301, 431, 441, 1760, 1779
BmrFI CCNGG 3 cut(s) 164, 446, 1775
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 1 cut(s) 717
BmtI GCTAGC 1 cut(s) 1022
BmuI ACTGGG 1 cut(s) 25
BplI GAGNNNNNCTC 6 cut(s) 124, 156, 353, 385, 1616, 1648
Bpu10I CCTNAGC 1 cut(s) 1528
Bpu14I TTCGAA 3 cut(s) 81, 256, 560
BpuMI CCSGG 1 cut(s) 1775
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 1663
BsaJI CCNNGG 7 cut(s) 50, 121, 143, 163, 444, 653, 1058
BsaWI WCCGGW 2 cut(s) 307, 594
BsaXI ACNNNNNCTCC 4 cut(s) 297, 327, 1315, 1345
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 1352, 1405
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 2 cut(s) 31, 745
Bse21I CCTNAGG 1 cut(s) 1647
Bse3DI GCAATG 1 cut(s) 1272
BseBI CCWGG 2 cut(s) 164, 446
BseDI CCNNGG 7 cut(s) 50, 121, 143, 163, 444, 653, 1058
BseGI GGATG 2 cut(s) 732, 813
BseLI CCNNNNNNNGG 3 cut(s) 387, 1352, 1405
BseMI GCAATG 1 cut(s) 1272
BseMII CTCAG 6 cut(s) 122, 144, 240, 1492, 1661, 1671
BseNI ACTGG 2 cut(s) 31, 745
BseSI GKGCMC 1 cut(s) 646
Bsh1236I CGCG 2 cut(s) 1678, 1804
BshFI GGCC 3 cut(s) 49, 139, 1871
BshNI GGYRCC 3 cut(s) 113, 429, 1777
BshTI ACCGGT 1 cut(s) 594
BsiHKAI GWGCWC 5 cut(s) 315, 521, 646, 754, 1479
BsiSI CCGG 4 cut(s) 308, 595, 1775, 1887
BslFI GGGAC 3 cut(s) 285, 295, 1772
BslI CCNNNNNNNGG 3 cut(s) 387, 1352, 1405
BsmAI GTCTC 3 cut(s) 694, 1411, 1663
BsmFI GGGAC 3 cut(s) 285, 295, 1772
BsnI GGCC 3 cut(s) 49, 139, 1871
Bso31I GGTCTC 1 cut(s) 1663
Bsp119I TTCGAA 3 cut(s) 81, 256, 560
Bsp1286I GDGCHC 5 cut(s) 315, 521, 646, 754, 1479
Bsp143I GATC 3 cut(s) 439, 1383, 1511
BspACI CCGC 4 cut(s) 4, 55, 136, 1873
BspANI GGCC 3 cut(s) 49, 139, 1871
BspCNI CTCAG 6 cut(s) 123, 145, 241, 1491, 1660, 1670
BspFNI CGCG 2 cut(s) 1678, 1804
BspLI GGNNCC 9 cut(s) 115, 283, 284, 300, 301, 431, 441, 1760, 1779
BspOI GCTAGC 1 cut(s) 1022
BspPI GGATC 3 cut(s) 434, 447, 1378
BspT104I TTCGAA 3 cut(s) 81, 256, 560
BspT107I GGYRCC 3 cut(s) 113, 429, 1777
BspTI CTTAAG 1 cut(s) 892
BspTNI GGTCTC 1 cut(s) 1663
BsrDI GCAATG 1 cut(s) 1272
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 2 cut(s) 31, 745
BssAI RCCGGY 1 cut(s) 594
BssECI CCNNGG 7 cut(s) 50, 121, 143, 163, 444, 653, 1058
BssMI GATC 3 cut(s) 439, 1383, 1511
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 5 cut(s) 50, 121, 143, 653, 1058
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 2 cut(s) 164, 446
Bst4CI ACNGT 2 cut(s) 845, 1763
Bst6I CTCTTC 2 cut(s) 2, 582
BstACI GRCGYC 1 cut(s) 114
BstAFI CTTAAG 1 cut(s) 892
BstAPI GCANNNNNTGC 1 cut(s) 460
BstBI TTCGAA 3 cut(s) 81, 256, 560
BstC8I GCNNGC 3 cut(s) 732, 1020, 1873
BstDEI CTNAG 8 cut(s) 131, 153, 249, 1439, 1478, 1528, 1647, 1657
BstF5I GGATG 2 cut(s) 732, 813
BstFNI CGCG 2 cut(s) 1678, 1804
BstH2I RGCGCY 4 cut(s) 117, 129, 151, 1041
BstHHI GCGC 6 cut(s) 116, 128, 150, 454, 1040, 1680
BstKTI GATC 3 cut(s) 442, 1386, 1514
BstMAI GTCTC 3 cut(s) 694, 1411, 1663
BstMBI GATC 3 cut(s) 439, 1383, 1511
BstMWI GCNNNNNNNGC 2 cut(s) 145, 460
BstNI CCWGG 2 cut(s) 164, 446
BstNSI RCATGY 3 cut(s) 228, 424, 734
BstSCI CCNGG 3 cut(s) 162, 444, 1773
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 2 cut(s) 1678, 1804
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
Bsu36I CCTNAGG 1 cut(s) 1647
BsuI GTATCC 1 cut(s) 938
BsuRI GGCC 3 cut(s) 49, 139, 1871
BtgZI GCGATG 1 cut(s) 1118
BtrI CACGTC 3 cut(s) 1732, 1744, 1811
BtsCI GGATG 2 cut(s) 732, 813
BtsIMutI CAGTG 1 cut(s) 38
Cac8I GCNNGC 3 cut(s) 732, 1020, 1873
CfoI GCGC 6 cut(s) 116, 128, 150, 454, 1040, 1680
Cfr10I RCCGGY 1 cut(s) 594
Cfr13I GGNCC 4 cut(s) 282, 299, 1759, 1870
CseI GACGC 1 cut(s) 1793
Csp6I GTAC 3 cut(s) 430, 957, 1778
CspAI ACCGGT 1 cut(s) 594
CspCI CAANNNNNGTGG 2 cut(s) 804, 839
CviAII CATG 6 cut(s) 225, 234, 278, 421, 731, 986
CviQI GTAC 3 cut(s) 430, 957, 1778
DdeI CTNAG 8 cut(s) 131, 153, 249, 1439, 1478, 1528, 1647, 1657
DinI GGCGCC 1 cut(s) 115
DpnI GATC 3 cut(s) 441, 1385, 1513
DpnII GATC 3 cut(s) 439, 1383, 1511
DraI TTTAAA 3 cut(s) 210, 862, 927
Eam1104I CTCTTC 2 cut(s) 2, 582
EarI CTCTTC 2 cut(s) 2, 582
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 3 cut(s) 313, 519, 1477
Eco130I CCWWGG 5 cut(s) 50, 121, 143, 653, 1058
Eco24I GRGCYC 3 cut(s) 315, 521, 1479
Eco31I GGTCTC 1 cut(s) 1663
Eco47I GGWCC 3 cut(s) 282, 299, 1759
Eco53kI GAGCTC 3 cut(s) 313, 519, 1477
Eco81I CCTNAGG 1 cut(s) 1647
EcoICRI GAGCTC 3 cut(s) 313, 519, 1477
EcoO109I RGGNCCY 2 cut(s) 282, 299
EcoRII CCWGG 2 cut(s) 162, 444
EcoT14I CCWWGG 5 cut(s) 50, 121, 143, 653, 1058
EcoT22I ATGCAT 1 cut(s) 732
EcoT38I GRGCYC 3 cut(s) 315, 521, 1479
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 5 cut(s) 50, 121, 143, 653, 1058
FaeI CATG 6 cut(s) 228, 237, 281, 424, 734, 989
FalI AAGNNNNNCTT 2 cut(s) 1694, 1726
FaqI GGGAC 3 cut(s) 285, 295, 1772
FatI CATG 6 cut(s) 224, 233, 277, 420, 730, 985
FauI CCCGC 1 cut(s) 1880
FauNDI CATATG 1 cut(s) 907
Fnu4HI GCNGC 1 cut(s) 137
FokI GGATG 2 cut(s) 739, 820
FriOI GRGCYC 3 cut(s) 315, 521, 1479
Fsp4HI GCNGC 1 cut(s) 137
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 6 cut(s) 115, 127, 149, 453, 1039, 1679
GluI GCNGC 1 cut(s) 137
HaeII RGCGCY 4 cut(s) 117, 129, 151, 1041
HaeIII GGCC 3 cut(s) 49, 139, 1871
HapII CCGG 4 cut(s) 308, 595, 1775, 1887
HgaI GACGC 1 cut(s) 1793
HhaI GCGC 6 cut(s) 116, 128, 150, 454, 1040, 1680
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 6 cut(s) 228, 237, 281, 424, 734, 989
Hin6I GCGC 6 cut(s) 114, 126, 148, 452, 1038, 1678
HinP1I GCGC 6 cut(s) 114, 126, 148, 452, 1038, 1678
HincII GTYRAC 1 cut(s) 1707
HindII GTYRAC 1 cut(s) 1707
HindIII AAGCTT 1 cut(s) 1525
HinfI GANTC 3 cut(s) 83, 352, 391
HpaI GTTAAC 1 cut(s) 1707
HpaII CCGG 4 cut(s) 308, 595, 1775, 1887
Hpy166II GTNNAC 4 cut(s) 644, 1363, 1707, 1808
Hpy188I TCNGA 6 cut(s) 132, 154, 670, 738, 1481, 1660
Hpy188III TCNNGA 6 cut(s) 482, 1097, 1491, 1641, 1649, 1739
Hpy8I GTNNAC 4 cut(s) 644, 1363, 1707, 1808
Hpy99I CGWCG 2 cut(s) 298, 1767
HpyAV CCTTC 5 cut(s) 541, 717, 1442, 1476, 1726
HpyCH4III ACNGT 2 cut(s) 845, 1763
HpyCH4IV ACGT 5 cut(s) 20, 1731, 1743, 1810, 1834
HpyCH4V TGCA 8 cut(s) 228, 424, 463, 644, 730, 1168, 1178, 1281
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 460
HpyF3I CTNAG 8 cut(s) 131, 153, 249, 1439, 1478, 1528, 1647, 1657
HpySE526I ACGT 5 cut(s) 20, 1731, 1743, 1810, 1834
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 6 cut(s) 228, 237, 281, 424, 734, 989
HspAI GCGC 6 cut(s) 114, 126, 148, 452, 1038, 1678
KasI GGCGCC 1 cut(s) 113
KflI GGGWCCC 2 cut(s) 282, 299
KpnI GGTACC 2 cut(s) 433, 1781
KspAI GTTAAC 1 cut(s) 1707
Kzo9I GATC 3 cut(s) 439, 1383, 1511
LmnI GCTCC 4 cut(s) 103, 310, 318, 516
LweI GCATC 1 cut(s) 717
MaeII ACGT 5 cut(s) 20, 1731, 1743, 1810, 1834
MaeIII GTNAC 5 cut(s) 243, 1025, 1321, 1732, 1811
MalI GATC 3 cut(s) 441, 1385, 1513
MboI GATC 3 cut(s) 439, 1383, 1511
MboII GAAGA 3 cut(s) 19, 180, 599
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 5 cut(s) 315, 521, 646, 754, 1479
MluI ACGCGT 1 cut(s) 1802
Mly113I GGCGCC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 922, 1855
Mph1103I ATGCAT 1 cut(s) 732
MroXI GAANNNNTTC 2 cut(s) 172, 1486
MspCI CTTAAG 1 cut(s) 892
MspI CCGG 4 cut(s) 308, 595, 1775, 1887
MspR9I CCNGG 3 cut(s) 164, 446, 1775
MvaI CCWGG 2 cut(s) 164, 446
MvnI CGCG 2 cut(s) 1678, 1804
MwoI GCNNNNNNNGC 2 cut(s) 145, 460
NarI GGCGCC 1 cut(s) 114
NciI CCSGG 1 cut(s) 1775
NdeI CATATG 1 cut(s) 907
NdeII GATC 3 cut(s) 439, 1383, 1511
NheI GCTAGC 1 cut(s) 1018
NlaIII CATG 6 cut(s) 228, 237, 281, 424, 734, 989
NlaIV GGNNCC 9 cut(s) 115, 283, 284, 300, 301, 431, 441, 1760, 1779
NmuCI GTSAC 4 cut(s) 1025, 1321, 1732, 1811
NsbI TGCGCA 1 cut(s) 453
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 3 cut(s) 228, 424, 734
NspV TTCGAA 3 cut(s) 81, 256, 560
PaeI GCATGC 1 cut(s) 734
PdmI GAANNNNTTC 2 cut(s) 172, 1486
PfeI GAWTC 3 cut(s) 83, 352, 391
PinAI ACCGGT 1 cut(s) 594
PkrI GCNGC 1 cut(s) 138
PluTI GGCGCC 1 cut(s) 117
PpuMI RGGWCCY 2 cut(s) 282, 299
PshBI ATTAAT 1 cut(s) 266
Psp124BI GAGCTC 3 cut(s) 315, 521, 1479
Psp5II RGGWCCY 2 cut(s) 282, 299
Psp6I CCWGG 2 cut(s) 162, 444
PspGI CCWGG 2 cut(s) 162, 444
PspN4I GGNNCC 9 cut(s) 115, 283, 284, 300, 301, 431, 441, 1760, 1779
PspPI GGNCC 4 cut(s) 282, 299, 1759, 1870
PspPPI RGGWCCY 2 cut(s) 282, 299
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 3 cut(s) 431, 958, 1779
RsaNI GTAC 3 cut(s) 430, 957, 1778
SacI GAGCTC 3 cut(s) 315, 521, 1479
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 3 cut(s) 439, 1383, 1511
Sau96I GGNCC 4 cut(s) 282, 299, 1759, 1870
ScrFI CCNGG 3 cut(s) 164, 446, 1775
SduI GDGCHC 5 cut(s) 315, 521, 646, 754, 1479
SfaNI GCATC 1 cut(s) 717
SfoI GGCGCC 1 cut(s) 115
SfuI TTCGAA 3 cut(s) 81, 256, 560
SinI GGWCC 3 cut(s) 282, 299, 1759
SmlI CTYRAG 1 cut(s) 892
SmoI CTYRAG 1 cut(s) 892
SpeI ACTAGT 1 cut(s) 1201
SphI GCATGC 1 cut(s) 734
SsiI CCGC 4 cut(s) 4, 55, 136, 1873
SspDI GGCGCC 1 cut(s) 113
SspI AATATT 2 cut(s) 1188, 1302
SstI GAGCTC 3 cut(s) 315, 521, 1479
StyD4I CCNGG 3 cut(s) 162, 444, 1773
StyI CCWWGG 5 cut(s) 50, 121, 143, 653, 1058
TaaI ACNGT 2 cut(s) 845, 1763
TaiI ACGT 5 cut(s) 23, 1734, 1746, 1813, 1837
TatI WGTACW 1 cut(s) 956
TauI GCSGC 1 cut(s) 139
TfiI GAWTC 3 cut(s) 83, 352, 391
TscAI CASTG 1 cut(s) 38
TseFI GTSAC 4 cut(s) 1025, 1321, 1732, 1811
Tsp45I GTSAC 4 cut(s) 1025, 1321, 1732, 1811
TspDTI ATGAA 9 cut(s) 266, 728, 819, 983, 1002, 1121, 1150, 1227, 1515
TspRI CASTG 1 cut(s) 38
Vha464I CTTAAG 1 cut(s) 892
VneI GTGCAC 1 cut(s) 642
VpaK11BI GGWCC 3 cut(s) 282, 299, 1759
VspI ATTAAT 1 cut(s) 266
XbaI TCTAGA 1 cut(s) 1490
XceI RCATGY 3 cut(s) 228, 424, 734
XmaJI CCTAGG 4 cut(s) 50, 121, 143, 653
XmnI GAANNNNTTC 2 cut(s) 172, 1486
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.