pycom07g12130

BON1-associated protein 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0019785
Physical Location & Seq
Forward (+)
13441074 .. 13444175
3102 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12130.6

Sequence Viewer

Length: 2205 bp
ATGAAAGCACCAATTTGTGGTAGCAAATTTTTGTCGCCTTTGATCGTGATCGAAAATGTACCTACAAAACAATCAACATCTTTGATCAAAGACCAAAGCCTCATGCTCAATTTCGATTCCATAATAAACGCATTTATGCGAAGAGTTGAGTTTCGTAACAAATCGTGTAAATTCAATTCCAAGCATGAATTGAGAAAATTCTCCAACCATGCCACACTCTACAATTGGATTTCCTCTACAATTAGTTGCCACTTTATTCACCACGTGATAAGTCAAAGATGTTACAACTTCAGTGATCATACTATAAATGTAACAGTCATACCAAAAAATTTCTCCGCACGATTATTTAAAAATAATGTAAGAACAAGTTGCTTGAAAGTTTCCCTCCGCTGTAAGAAGCGTGAACCAGAGTCAACTTGGAATCCATTCAACATTCGGCTCGTATTGTATTTCATCGTCCAATTTGAATTCCACAGAAACCACGTCGGTTTTCTCTCCTTTCTCACACCCTTCCCTCAACACTCCTATATAACCTCCTACCCACACCTCTTCCCTCTCAACGGCTCTCACAATTCCCCTAACCTAACACAAGCAATTCATCAAACACTTGCGCGCACTCTTCATCGATCATGGCAGCCAATTCCTGGACGCTCGAAATCAAGCCAGTTCCGCTCCATGGACATGAACATCGATGGCAGCGCTTACCCACGGTGGAACGAGAAGCTCGTGCTCAACTTCCCAACGCACTCCAAGTCCATCACGGTGGAGGTCCAATGCAAGACTTCGTCCGGCGTCAGGACGATCGGGACGGCGACGGCGTCGGATTTTGTTGGCGGGTATGTGCCAGAGGGTTGCATGCTTTTTTTGAGTTACAGGCTGAGAGATCACAAGGGGGAGAGGAATGGGATTATTAATATTTCCGTGAGGATGAAGGTTCATGAGATGTATGCTTGTGCAAGTTCAACGATGTGGTCTCACTCGCAGATGGGGTTTCCGTTCGTATTTGGTGTGGTGCCTATCAGAAAAATTATTGAAGATCCAAGGCACAATTTCTTGTGCGTAAATATTGCAGGATTTAGACTTTTGGTTGAGGAGAATACAAACTCAAGGGCTTTTAAATTTTCTCAATATCTTATAGTGATTGTAATTATATATTATCAAAGCCCACCATTTCCTCAATTTTTCTGCTTGCACAACACATTTACAATGTGCCGGCACCAAGCAGCCGACAACAGATATGGTGAAGTGCTTATTTATATTTTTTCACCCTACTATTTTATCCAATTTTTTGTCTACTTAAATCACTTAACACATTTTAGGCTAATTTACCTTCTAAAGTCAACTCTGACACACTATGACTCGGAATGTCCACTAGGACCTTTAATGGAGCTTGATGTGGAAAATGGAATAAATTTAAACCTAAAAGTGACTAAAGACAAGAGTGCGCTTACAGTAGTGTGGTCACCAAGAATCCTCGTCGATACGAATTTTCAGCAGCTAAAACCTGGAGGGGCAAAAAACAGAAAACGTGAGTGGGCAAAAACAAAGCTTTTTGAAACCATTTCTCTTTCCAAAATAATAATAACCCATCACCGTAAAACCCGTAATACTTCTCAGAAAATAGTTATACATACATATGTAGAAATCATGCTCGAGAGTAGTGAAAACCAAGTATATACCATGTCAAGTGACCTGTACGGCTTAACCTATAGCTCATCAAATATGCCTGCACATAAGTCGGAACCACCTATTGTGGTCTGTACGACAGGACTGGGTGTAAATAAGTACGTTCAAGTGCTACAATCACAAGTAGGTTGTGCGAATAATCGCGAGTCACCTACAAGTCGGAACCACCTAATGTGTGCAGGAGGTGAATATCATGTGAAGAACTGTGCCCTGGCCACGGGCGAGAGCACTAACACTGAGGTGCAGGAGAATGACTTGCCTGGGCATTCGCAGCGTCATGCAGTAATATGTATATTTATACTAATTCATATACTAAAATGTAATGCAATTGACATGGCATTTAAAAACTTAAATCGATTTAAAACAATTTTTGGGAAAATGGAAAAGATATATATACATATATATAGAAAACCAAAAGCCCACTCACCTGAAGTCTCGCTGCCTAGAACAATTATCAACCCACATCTCATAATATTTATCAATATAACACATAACTTACATAAAACAAATCCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

735

Amino Acids

84.5

Weight (kDa)

9.59

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019785)

Species Orthologous Gene IDs
malus_domestica MD07G1185500.v1.1
pyrus_communis pycom01g04080 pycom05g08400 pycom07g12130 pycom11g13980 pycom17g16240 pycom17g17130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1012, 1215
AccB7I CCANNNNNTGG 2 cut(s) 17, 644
AccBSI CCGCTC 1 cut(s) 672
AccI GTMKAC 1 cut(s) 1293
AccII CGCG 2 cut(s) 613, 1830
AciI CCGC 4 cut(s) 336, 388, 670, 834
AclWI GGATC 1 cut(s) 1031
AcoI YGGCCR 1 cut(s) 1899
AcsI RAATTY 8 cut(s) 26, 170, 197, 328, 467, 1118, 1411, 1486
AcuI CTGAAG 2 cut(s) 274, 2136
AcvI CACGTG 1 cut(s) 265
AcyI GRCGYC 2 cut(s) 792, 818
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 4 cut(s) 60, 1697, 1762, 1787
AfeI AGCGCT 1 cut(s) 700
AfiI CCNNNNNNNGG 6 cut(s) 17, 560, 644, 676, 795, 1903
AgsI TTSAA 8 cut(s) 175, 376, 430, 467, 963, 1034, 1556, 1793
AjiI CACGTC 1 cut(s) 484
AjnI CCWGG 4 cut(s) 643, 1504, 1896, 1945
AleI CACNNNNGTG 1 cut(s) 1925
AluBI AGCT 5 cut(s) 724, 1390, 1498, 1549, 1713
AluI AGCT 5 cut(s) 724, 1390, 1498, 1549, 1713
Alw21I GWGCWC 2 cut(s) 732, 1916
Alw26I GTCTC 2 cut(s) 978, 2125
AlwI GGATC 1 cut(s) 1031
Ama87I CYCGRG 1 cut(s) 1652
Aor51HI AGCGCT 1 cut(s) 700
AoxI GGCC 1 cut(s) 1899
ApeKI GCWGC 6 cut(s) 634, 696, 1223, 1495, 1957, 2125
ApoI RAATTY 8 cut(s) 26, 170, 197, 328, 467, 1118, 1411, 1486
AseI ATTAAT 1 cut(s) 912
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 4 cut(s) 613, 615, 701, 1447
AspS9I GGNCC 2 cut(s) 769, 1376
AsuHPI GGTGA 8 cut(s) 251, 1253, 1257, 1455, 1583, 1827, 1883, 2103
AvaI CYCGRG 1 cut(s) 1652
AvaII GGWCC 2 cut(s) 769, 1376
BaeGI GKGCMC 1 cut(s) 1897
BalI TGGCCA 1 cut(s) 1901
BanI GGYRCC 2 cut(s) 1012, 1215
BauI CACGAG 1 cut(s) 725
BbrPI CACGTG 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 732, 1916
BbvI GCAGC 6 cut(s) 646, 708, 1235, 1507, 1969, 2112
BccI CCATC 4 cut(s) 686, 764, 979, 1596
BceAI ACGGC 4 cut(s) 577, 825, 831, 1714
BcgI CGANNNNNNTGC 2 cut(s) 1719, 1753
BciT130I CCWGG 4 cut(s) 645, 1506, 1898, 1947
BclI TGATCA 2 cut(s) 84, 295
BcoDI GTCTC 2 cut(s) 978, 2125
BfaI CTAG 2 cut(s) 1373, 2130
BfmI CTRYAG 1 cut(s) 1708
BfoI RGCGCY 1 cut(s) 702
BisI GCNGC 6 cut(s) 635, 697, 1224, 1496, 1958, 2126
BlsI GCNGC 6 cut(s) 636, 698, 1225, 1497, 1959, 2127
Bme1390I CCNGG 4 cut(s) 645, 1506, 1898, 1947
Bme18I GGWCC 2 cut(s) 769, 1376
BmeT110I CYCGRG 1 cut(s) 1652
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 2 cut(s) 769, 1376
BmiI GGNNCC 4 cut(s) 1014, 1217, 1743, 1850
BmrFI CCNGG 4 cut(s) 645, 1506, 1898, 1947
BmrI ACTGGG 1 cut(s) 1781
BmuI ACTGGG 1 cut(s) 1781
BpmI CTGGAG 1 cut(s) 1527
BpuEI CTTGAG 1 cut(s) 1090
Bsa29I ATCGAT 3 cut(s) 625, 690, 2041
BsaAI YACGTR 1 cut(s) 265
BsaBI GATNNNNATC 1 cut(s) 47
BsaHI GRCGYC 2 cut(s) 792, 818
BsaI GGTCTC 1 cut(s) 978
BsaJI CCNNGG 6 cut(s) 675, 707, 1040, 1896, 1902, 1946
Bsc4I CCNNNNNNNGG 6 cut(s) 17, 560, 644, 676, 795, 1903
Bse118I RCCGGY 1 cut(s) 1212
Bse1I ACTGG 2 cut(s) 664, 1776
Bse8I GATNNNNATC 1 cut(s) 47
BseBI CCWGG 4 cut(s) 645, 1506, 1898, 1947
BseCI ATCGAT 3 cut(s) 625, 690, 2041
BseDI CCNNGG 6 cut(s) 675, 707, 1040, 1896, 1902, 1946
BseGI GGATG 1 cut(s) 933
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 6 cut(s) 17, 560, 644, 676, 795, 1903
BseMII CTCAG 3 cut(s) 869, 1628, 1914
BseNI ACTGG 2 cut(s) 664, 1776
BsePI GCGCGC 1 cut(s) 611
BseRI GAGGAG 1 cut(s) 1106
BseSI GKGCMC 1 cut(s) 1897
BseXI GCAGC 6 cut(s) 646, 708, 1235, 1507, 1969, 2112
BsgI GTGCAG 3 cut(s) 1713, 1884, 1949
Bsh1236I CGCG 2 cut(s) 613, 1830
Bsh1285I CGRYCG 1 cut(s) 804
BshFI GGCC 1 cut(s) 1901
BshNI GGYRCC 2 cut(s) 1012, 1215
BshVI ATCGAT 3 cut(s) 625, 690, 2041
BsiEI CGRYCG 1 cut(s) 804
BsiHKAI GWGCWC 2 cut(s) 732, 1916
BsiHKCI CYCGRG 1 cut(s) 1652
BsiSI CCGG 2 cut(s) 789, 1213
BslFI GGGAC 1 cut(s) 820
BslI CCNNNNNNNGG 6 cut(s) 17, 560, 644, 676, 795, 1903
BsmAI GTCTC 2 cut(s) 978, 2125
BsmFI GGGAC 1 cut(s) 820
BsmI GAATGC 1 cut(s) 1951
BsnI GGCC 1 cut(s) 1901
Bso31I GGTCTC 1 cut(s) 978
BsoBI CYCGRG 1 cut(s) 1652
Bsp1286I GDGCHC 3 cut(s) 732, 1897, 1916
Bsp143I GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
Bsp19I CCATGG 1 cut(s) 675
Bsp68I TCGCGA 1 cut(s) 1830
BspACI CCGC 4 cut(s) 336, 388, 670, 834
BspANI GGCC 1 cut(s) 1901
BspCNI CTCAG 3 cut(s) 870, 1627, 1915
BspDI ATCGAT 3 cut(s) 625, 690, 2041
BspFNI CGCG 2 cut(s) 613, 1830
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 4 cut(s) 1014, 1217, 1743, 1850
BspPI GGATC 1 cut(s) 1031
BspT107I GGYRCC 2 cut(s) 1012, 1215
BspTNI GGTCTC 1 cut(s) 978
BsrBI CCGCTC 1 cut(s) 672
BsrFI RCCGGY 1 cut(s) 1212
BsrI ACTGG 2 cut(s) 664, 1776
BssAI RCCGGY 1 cut(s) 1212
BssECI CCNNGG 6 cut(s) 675, 707, 1040, 1896, 1902, 1946
BssHII GCGCGC 1 cut(s) 611
BssMI GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
BssNI GRCGYC 2 cut(s) 792, 818
BssSI CACGAG 1 cut(s) 725
BssT1I CCWWGG 2 cut(s) 675, 1040
Bst2BI CACGAG 1 cut(s) 725
Bst2UI CCWGG 4 cut(s) 645, 1506, 1898, 1947
Bst4CI ACNGT 6 cut(s) 316, 711, 763, 1453, 1595, 1892
Bst6I CTCTTC 3 cut(s) 136, 554, 624
BstACI GRCGYC 2 cut(s) 792, 818
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 5 cut(s) 613, 857, 1190, 1214, 1728
BstDEI CTNAG 3 cut(s) 878, 1614, 1923
BstDSI CCRYGG 3 cut(s) 675, 707, 1902
BstEII GGTNACC 1 cut(s) 1461
BstF5I GGATG 1 cut(s) 933
BstFNI CGCG 2 cut(s) 613, 1830
BstH2I RGCGCY 1 cut(s) 702
BstHHI GCGC 4 cut(s) 613, 615, 701, 1447
BstKTI GATC 8 cut(s) 45, 51, 87, 298, 629, 804, 886, 1039
BstMAI GTCTC 2 cut(s) 978, 2125
BstMBI GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
BstMCI CGRYCG 1 cut(s) 804
BstMWI GCNNNNNNNGC 2 cut(s) 669, 1957
BstNI CCWGG 4 cut(s) 645, 1506, 1898, 1947
BstNSI RCATGY 1 cut(s) 859
BstPI GGTNACC 1 cut(s) 1461
BstSCI CCNGG 4 cut(s) 643, 1504, 1896, 1945
BstSFI CTRYAG 1 cut(s) 1708
BstSLI GKGCMC 1 cut(s) 1897
BstUI CGCG 2 cut(s) 613, 1830
BstV1I GCAGC 6 cut(s) 646, 708, 1235, 1507, 1969, 2112
BstX2I RGATCY 1 cut(s) 1036
BstXI CCANNNNNNTGG 1 cut(s) 763
BstYI RGATCY 1 cut(s) 1036
Bsu15I ATCGAT 3 cut(s) 625, 690, 2041
BsuRI GGCC 1 cut(s) 1901
BsuTUI ATCGAT 3 cut(s) 625, 690, 2041
BtgI CCRYGG 3 cut(s) 675, 707, 1902
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 1 cut(s) 933
BtsIMutI CAGTG 2 cut(s) 298, 1920
BtuMI TCGCGA 1 cut(s) 1830
Cac8I GCNNGC 5 cut(s) 613, 857, 1190, 1214, 1728
CciI TCATGA 1 cut(s) 937
CfoI GCGC 4 cut(s) 613, 615, 701, 1447
Cfr10I RCCGGY 1 cut(s) 1212
Cfr13I GGNCC 2 cut(s) 769, 1376
ClaI ATCGAT 3 cut(s) 625, 690, 2041
CseI GACGC 4 cut(s) 657, 781, 807, 1949
Csp6I GTAC 4 cut(s) 59, 1696, 1761, 1786
CviQI GTAC 4 cut(s) 59, 1696, 1761, 1786
DdeI CTNAG 3 cut(s) 878, 1614, 1923
DpnI GATC 8 cut(s) 44, 50, 86, 297, 628, 803, 885, 1038
DpnII GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
DraI TTTAAA 5 cut(s) 349, 1117, 1416, 2029, 2047
DraIII CACNNNGTG 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 1899
Eam1104I CTCTTC 3 cut(s) 136, 554, 624
EarI CTCTTC 3 cut(s) 136, 554, 624
Eco130I CCWWGG 2 cut(s) 675, 1040
Eco31I GGTCTC 1 cut(s) 978
Eco47I GGWCC 2 cut(s) 769, 1376
Eco47III AGCGCT 1 cut(s) 700
Eco57I CTGAAG 2 cut(s) 274, 2136
Eco72I CACGTG 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 1652
Eco91I GGTNACC 1 cut(s) 1461
EcoO109I RGGNCCY 1 cut(s) 1376
EcoO65I GGTNACC 1 cut(s) 1461
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 4 cut(s) 643, 1504, 1896, 1945
EcoT14I CCWWGG 2 cut(s) 675, 1040
ErhI CCWWGG 2 cut(s) 675, 1040
FaqI GGGAC 1 cut(s) 820
FauI CCCGC 1 cut(s) 827
FauNDI CATATG 1 cut(s) 1636
FbaI TGATCA 2 cut(s) 84, 295
FblI GTMKAC 1 cut(s) 1293
Fnu4HI GCNGC 6 cut(s) 635, 697, 1224, 1496, 1958, 2126
FokI GGATG 1 cut(s) 940
Fsp4HI GCNGC 6 cut(s) 635, 697, 1224, 1496, 1958, 2126
FspBI CTAG 2 cut(s) 1373, 2130
GlaI GCGC 4 cut(s) 612, 614, 700, 1446
GluI GCNGC 6 cut(s) 635, 697, 1224, 1496, 1958, 2126
GsuI CTGGAG 1 cut(s) 1527
HaeII RGCGCY 1 cut(s) 702
HaeIII GGCC 1 cut(s) 1901
HapII CCGG 2 cut(s) 789, 1213
HgaI GACGC 4 cut(s) 657, 781, 807, 1949
HhaI GCGC 4 cut(s) 613, 615, 701, 1447
Hin1I GRCGYC 2 cut(s) 792, 818
Hin6I GCGC 4 cut(s) 611, 613, 699, 1445
HinP1I GCGC 4 cut(s) 611, 613, 699, 1445
HincII GTYRAC 2 cut(s) 414, 1341
HindII GTYRAC 2 cut(s) 414, 1341
HindIII AAGCTT 1 cut(s) 1547
HinfI GANTC 6 cut(s) 116, 410, 421, 1358, 1470, 1832
HpaII CCGG 2 cut(s) 789, 1213
HphI GGTGA 8 cut(s) 251, 1253, 1257, 1455, 1583, 1827, 1883, 2103
Hpy166II GTNNAC 5 cut(s) 404, 414, 1294, 1341, 1370
Hpy188I TCNGA 7 cut(s) 823, 1022, 1347, 1363, 1617, 1741, 1848
Hpy188III TCNNGA 6 cut(s) 46, 796, 805, 938, 1654, 1829
Hpy8I GTNNAC 5 cut(s) 404, 414, 1294, 1341, 1370
Hpy99I CGWCG 4 cut(s) 488, 817, 823, 1481
HpyAV CCTTC 3 cut(s) 520, 925, 1340
HpyCH4III ACNGT 6 cut(s) 316, 711, 763, 1453, 1595, 1892
HpyCH4IV ACGT 4 cut(s) 264, 483, 1528, 1788
HpyF10VI GCNNNNNNNGC 2 cut(s) 669, 1957
HpyF3I CTNAG 3 cut(s) 878, 1614, 1923
HpySE526I ACGT 4 cut(s) 264, 483, 1528, 1788
Hsp92I GRCGYC 2 cut(s) 792, 818
HspAI GCGC 4 cut(s) 611, 613, 699, 1445
KroI GCCGGC 1 cut(s) 1212
KroNI GCCGGC 1 cut(s) 1214
Ksp22I TGATCA 2 cut(s) 84, 295
Kzo9I GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
LmnI GCTCC 2 cut(s) 677, 1387
Lsp1109I GCAGC 6 cut(s) 646, 708, 1235, 1507, 1969, 2112
MaeI CTAG 2 cut(s) 1373, 2130
MaeII ACGT 4 cut(s) 264, 483, 1528, 1788
MaeIII GTNAC 8 cut(s) 155, 281, 310, 869, 1426, 1461, 1688, 1833
MalI GATC 8 cut(s) 44, 50, 86, 297, 628, 803, 885, 1038
MbiI CCGCTC 1 cut(s) 672
MboI GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
MboII GAAGA 5 cut(s) 153, 541, 611, 1046, 1897
MfeI CAATTG 2 cut(s) 223, 2013
MflI RGATCY 1 cut(s) 1036
MhlI GDGCHC 3 cut(s) 732, 1897, 1916
MlsI TGGCCA 1 cut(s) 1901
MluNI TGGCCA 1 cut(s) 1901
MlyI GAGTC 3 cut(s) 419, 1352, 1841
MmeI TCCRAC 4 cut(s) 228, 801, 1719, 1826
Mox20I TGGCCA 1 cut(s) 1901
MroNI GCCGGC 1 cut(s) 1212
MroXI GAANNNNTTC 1 cut(s) 425
MscI TGGCCA 1 cut(s) 1901
MslI CAYNNNNRTG 4 cut(s) 680, 761, 1635, 1925
Msp20I TGGCCA 1 cut(s) 1901
MspA1I CMGCKG 1 cut(s) 390
MspI CCGG 2 cut(s) 789, 1213
MspR9I CCNGG 4 cut(s) 645, 1506, 1898, 1947
MunI CAATTG 2 cut(s) 223, 2013
Mva1269I GAATGC 1 cut(s) 1951
MvaI CCWGG 4 cut(s) 645, 1506, 1898, 1947
MvnI CGCG 2 cut(s) 613, 1830
MwoI GCNNNNNNNGC 2 cut(s) 669, 1957
NaeI GCCGGC 1 cut(s) 1214
NcoI CCATGG 1 cut(s) 675
NdeI CATATG 1 cut(s) 1636
NdeII GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
NgoMIV GCCGGC 1 cut(s) 1212
NlaIV GGNNCC 4 cut(s) 1014, 1217, 1743, 1850
NmuCI GTSAC 4 cut(s) 1426, 1461, 1688, 1833
NruI TCGCGA 1 cut(s) 1830
NspI RCATGY 1 cut(s) 859
OliI CACNNNNGTG 1 cut(s) 1925
PaeI GCATGC 1 cut(s) 859
PaeR7I CTCGAG 1 cut(s) 1652
PagI TCATGA 1 cut(s) 937
PauI GCGCGC 1 cut(s) 611
PcsI WCGNNNNNNNCGW 2 cut(s) 723, 815
PctI GAATGC 1 cut(s) 1951
PdiI GCCGGC 1 cut(s) 1214
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 3 cut(s) 116, 421, 1470
PflFI GACNNNGTC 2 cut(s) 784, 817
PflMI CCANNNNNTGG 2 cut(s) 17, 644
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 6 cut(s) 636, 698, 1225, 1497, 1959, 2127
Ple19I CGATCG 1 cut(s) 804
PleI GAGTC 3 cut(s) 418, 1352, 1840
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 418, 1352, 1840
Ppu21I YACGTR 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 1376
PshBI ATTAAT 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 4 cut(s) 643, 1504, 1896, 1945
PspCI CACGTG 1 cut(s) 265
PspEI GGTNACC 1 cut(s) 1461
PspGI CCWGG 4 cut(s) 643, 1504, 1896, 1945
PspN4I GGNNCC 4 cut(s) 1014, 1217, 1743, 1850
PspPI GGNCC 2 cut(s) 769, 1376
PspPPI RGGWCCY 1 cut(s) 1376
PsuI RGATCY 1 cut(s) 1036
PsyI GACNNNGTC 2 cut(s) 784, 817
PteI GCGCGC 1 cut(s) 611
PvuI CGATCG 1 cut(s) 804
RruI TCGCGA 1 cut(s) 1830
RsaI GTAC 4 cut(s) 60, 1697, 1762, 1787
RsaNI GTAC 4 cut(s) 59, 1696, 1761, 1786
RseI CAYNNNNRTG 4 cut(s) 680, 761, 1635, 1925
SatI GCNGC 6 cut(s) 635, 697, 1224, 1496, 1958, 2126
Sau3AI GATC 8 cut(s) 42, 48, 84, 295, 626, 801, 883, 1036
Sau96I GGNCC 2 cut(s) 769, 1376
SchI GAGTC 3 cut(s) 419, 1352, 1841
ScrFI CCNGG 4 cut(s) 645, 1506, 1898, 1947
SduI GDGCHC 3 cut(s) 732, 1897, 1916
SfcI CTRYAG 1 cut(s) 1708
Sfr274I CTCGAG 1 cut(s) 1652
SinI GGWCC 2 cut(s) 769, 1376
SlaI CTCGAG 1 cut(s) 1652
SmiMI CAYNNNNRTG 4 cut(s) 680, 761, 1635, 1925
SmlI CTYRAG 2 cut(s) 1105, 1652
SmoI CTYRAG 2 cut(s) 1105, 1652
SphI GCATGC 1 cut(s) 859
SsiI CCGC 4 cut(s) 336, 388, 670, 834
SspI AATATT 3 cut(s) 916, 1066, 2160
SspMI CTAG 2 cut(s) 1373, 2130
StyD4I CCNGG 4 cut(s) 643, 1504, 1896, 1945
StyI CCWWGG 2 cut(s) 675, 1040
TaaI ACNGT 6 cut(s) 316, 711, 763, 1453, 1595, 1892
TaiI ACGT 4 cut(s) 267, 486, 1531, 1791
TaqI TCGA 8 cut(s) 51, 114, 625, 653, 690, 1479, 1653, 2041
TfiI GAWTC 3 cut(s) 116, 421, 1470
TscAI CASTG 2 cut(s) 298, 1927
TseFI GTSAC 4 cut(s) 1426, 1461, 1688, 1833
TseI GCWGC 6 cut(s) 634, 696, 1223, 1495, 1957, 2125
Tsp45I GTSAC 4 cut(s) 1426, 1461, 1688, 1833
TspDTI ATGAA 9 cut(s) 17, 201, 442, 587, 611, 698, 926, 944, 1982
TspGWI ACGGA 2 cut(s) 910, 984
TspRI CASTG 2 cut(s) 298, 1927
Tth111I GACNNNGTC 2 cut(s) 784, 817
Van91I CCANNNNNTGG 2 cut(s) 17, 644
VpaK11BI GGWCC 2 cut(s) 769, 1376
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 8 cut(s) 26, 170, 197, 328, 467, 1118, 1411, 1486
XceI RCATGY 1 cut(s) 859
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
XhoI CTCGAG 1 cut(s) 1652
XmiI GTMKAC 1 cut(s) 1293
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 1373, 2130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.