Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom05g12680 |
| rosa_chinensis | RchiOBHm_Chr6g0279761 |
| rosa_laevigata | RLG00000013123 |
| rosa_multiflora | Rmu_sc0001719.1_g000017 |
| rosa_roxburghii | Rroxscaffold_7G00188690 |
| rosa_rugosa | Rorug06G0127600 |
| rosa_samantha | Rh6AG236000 Rh6BG240200 Rh6CG242300 Rh6DG233900 |
| rosa_wichuraiana | Rw6G020570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 118 |
| AciI | CCGC | 2 cut(s) | 290, 512 |
| AclWI | GGATC | 2 cut(s) | 174, 260 |
| AcsI | RAATTY | 1 cut(s) | 439 |
| AfaI | GTAC | 4 cut(s) | 30, 112, 126, 376 |
| AgsI | TTSAA | 3 cut(s) | 463, 541, 562 |
| AluBI | AGCT | 1 cut(s) | 556 |
| AluI | AGCT | 1 cut(s) | 556 |
| AlwI | GGATC | 2 cut(s) | 174, 260 |
| AoxI | GGCC | 1 cut(s) | 397 |
| ApoI | RAATTY | 1 cut(s) | 439 |
| AsuHPI | GGTGA | 1 cut(s) | 7 |
| BanI | GGYRCC | 1 cut(s) | 118 |
| BauI | CACGAG | 1 cut(s) | 345 |
| BccI | CCATC | 3 cut(s) | 134, 142, 573 |
| BceAI | ACGGC | 2 cut(s) | 56, 180 |
| BclI | TGATCA | 1 cut(s) | 226 |
| BfaI | CTAG | 2 cut(s) | 333, 567 |
| BfmI | CTRYAG | 1 cut(s) | 58 |
| BmiI | GGNNCC | 4 cut(s) | 120, 203, 330, 471 |
| BmsI | GCATC | 1 cut(s) | 356 |
| BsaAI | YACGTR | 1 cut(s) | 374 |
| BsaJI | CCNNGG | 1 cut(s) | 162 |
| Bse118I | RCCGGY | 1 cut(s) | 115 |
| BseDI | CCNNGG | 1 cut(s) | 162 |
| BseRI | GAGGAG | 1 cut(s) | 482 |
| BseYI | CCCAGC | 1 cut(s) | 433 |
| BsgI | GTGCAG | 1 cut(s) | 342 |
| BshFI | GGCC | 1 cut(s) | 399 |
| BshNI | GGYRCC | 1 cut(s) | 118 |
| BsiSI | CCGG | 1 cut(s) | 116 |
| BsmI | GAATGC | 1 cut(s) | 300 |
| BsnI | GGCC | 1 cut(s) | 399 |
| Bsp1407I | TGTACA | 1 cut(s) | 124 |
| Bsp143I | GATC | 3 cut(s) | 166, 226, 265 |
| BspACI | CCGC | 2 cut(s) | 290, 512 |
| BspANI | GGCC | 1 cut(s) | 399 |
| BspLI | GGNNCC | 4 cut(s) | 120, 203, 330, 471 |
| BspMAI | CTGCAG | 1 cut(s) | 62 |
| BspPI | GGATC | 2 cut(s) | 174, 260 |
| BspT107I | GGYRCC | 1 cut(s) | 118 |
| BsrFI | RCCGGY | 1 cut(s) | 115 |
| BsrGI | TGTACA | 1 cut(s) | 124 |
| BssAI | RCCGGY | 1 cut(s) | 115 |
| BssECI | CCNNGG | 1 cut(s) | 162 |
| BssMI | GATC | 3 cut(s) | 166, 226, 265 |
| BssSI | CACGAG | 1 cut(s) | 345 |
| BssT1I | CCWWGG | 1 cut(s) | 162 |
| Bst2BI | CACGAG | 1 cut(s) | 345 |
| Bst4CI | ACNGT | 3 cut(s) | 28, 129, 274 |
| Bst6I | CTCTTC | 1 cut(s) | 536 |
| BstAUI | TGTACA | 1 cut(s) | 124 |
| BstBAI | YACGTR | 1 cut(s) | 374 |
| BstC8I | GCNNGC | 2 cut(s) | 62, 92 |
| BstEII | GGTNACC | 1 cut(s) | 506 |
| BstKTI | GATC | 3 cut(s) | 169, 229, 268 |
| BstMBI | GATC | 3 cut(s) | 166, 226, 265 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 356 |
| BstNSI | RCATGY | 1 cut(s) | 94 |
| BstPI | GGTNACC | 1 cut(s) | 506 |
| BstSFI | CTRYAG | 1 cut(s) | 58 |
| BstSNI | TACGTA | 1 cut(s) | 374 |
| BstX2I | RGATCY | 1 cut(s) | 166 |
| BstYI | RGATCY | 1 cut(s) | 166 |
| BsuRI | GGCC | 1 cut(s) | 399 |
| Cac8I | GCNNGC | 2 cut(s) | 62, 92 |
| Cfr10I | RCCGGY | 1 cut(s) | 115 |
| Csp6I | GTAC | 4 cut(s) | 29, 111, 125, 375 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 366, 401 |
| CviAII | CATG | 2 cut(s) | 91, 571 |
| CviJI | RGCY | 5 cut(s) | 9, 310, 399, 472, 556 |
| CviKI_1 | RGCY | 5 cut(s) | 9, 310, 399, 472, 556 |
| CviQI | GTAC | 4 cut(s) | 29, 111, 125, 375 |
| DpnI | GATC | 3 cut(s) | 168, 228, 267 |
| DpnII | GATC | 3 cut(s) | 166, 226, 265 |
| Eam1104I | CTCTTC | 1 cut(s) | 536 |
| EarI | CTCTTC | 1 cut(s) | 536 |
| Eco105I | TACGTA | 1 cut(s) | 374 |
| Eco130I | CCWWGG | 1 cut(s) | 162 |
| Eco91I | GGTNACC | 1 cut(s) | 506 |
| EcoO65I | GGTNACC | 1 cut(s) | 506 |
| EcoT14I | CCWWGG | 1 cut(s) | 162 |
| EcoT22I | ATGCAT | 1 cut(s) | 371 |
| ErhI | CCWWGG | 1 cut(s) | 162 |
| FaeI | CATG | 2 cut(s) | 94, 574 |
| FatI | CATG | 2 cut(s) | 90, 570 |
| FauI | CCCGC | 2 cut(s) | 283, 519 |
| FbaI | TGATCA | 1 cut(s) | 226 |
| FspBI | CTAG | 2 cut(s) | 333, 567 |
| GsaI | CCCAGC | 1 cut(s) | 437 |
| HaeIII | GGCC | 1 cut(s) | 399 |
| HapII | CCGG | 1 cut(s) | 116 |
| Hin1II | CATG | 2 cut(s) | 94, 574 |
| HindIII | AAGCTT | 1 cut(s) | 554 |
| HinfI | GANTC | 1 cut(s) | 185 |
| HpaII | CCGG | 1 cut(s) | 116 |
| HphI | GGTGA | 1 cut(s) | 7 |
| Hpy166II | GTNNAC | 1 cut(s) | 113 |
| Hpy188I | TCNGA | 1 cut(s) | 87 |
| Hpy8I | GTNNAC | 1 cut(s) | 113 |
| Hpy99I | CGWCG | 1 cut(s) | 201 |
| HpyAV | CCTTC | 1 cut(s) | 146 |
| HpyCH4III | ACNGT | 3 cut(s) | 28, 129, 274 |
| HpyCH4IV | ACGT | 2 cut(s) | 373, 419 |
| HpyCH4V | TGCA | 5 cut(s) | 60, 94, 134, 359, 369 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 356 |
| HpySE526I | ACGT | 2 cut(s) | 373, 419 |
| Hsp92II | CATG | 2 cut(s) | 94, 574 |
| Ksp22I | TGATCA | 1 cut(s) | 226 |
| Kzo9I | GATC | 3 cut(s) | 166, 226, 265 |
| LmnI | GCTCC | 2 cut(s) | 303, 469 |
| LpnPI | CCDG | 7 cut(s) | 46, 70, 129, 135, 273, 311, 419 |
| LweI | GCATC | 1 cut(s) | 356 |
| MaeI | CTAG | 2 cut(s) | 333, 567 |
| MaeII | ACGT | 2 cut(s) | 373, 419 |
| MaeIII | GTNAC | 2 cut(s) | 73, 506 |
| MalI | GATC | 3 cut(s) | 168, 228, 267 |
| MboI | GATC | 3 cut(s) | 166, 226, 265 |
| MboII | GAAGA | 4 cut(s) | 33, 230, 487, 553 |
| MfeI | CAATTG | 1 cut(s) | 285 |
| MflI | RGATCY | 1 cut(s) | 166 |
| MluCI | AATT | 4 cut(s) | 81, 285, 302, 439 |
| MnlI | CCTC | 5 cut(s) | 42, 354, 421, 460, 537 |
| Mph1103I | ATGCAT | 1 cut(s) | 371 |
| MseI | TTAA | 1 cut(s) | 306 |
| MslI | CAYNNNNRTG | 1 cut(s) | 364 |
| MspI | CCGG | 1 cut(s) | 116 |
| MunI | CAATTG | 1 cut(s) | 285 |
| Mva1269I | GAATGC | 1 cut(s) | 300 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 356 |
| NdeII | GATC | 3 cut(s) | 166, 226, 265 |
| NlaIII | CATG | 2 cut(s) | 94, 574 |
| NlaIV | GGNNCC | 4 cut(s) | 120, 203, 330, 471 |
| NmuCI | GTSAC | 1 cut(s) | 73 |
| NsiI | ATGCAT | 1 cut(s) | 371 |
| NspI | RCATGY | 1 cut(s) | 94 |
| PaeI | GCATGC | 1 cut(s) | 94 |
| PctI | GAATGC | 1 cut(s) | 300 |
| PfeI | GAWTC | 1 cut(s) | 185 |
| Ppu21I | YACGTR | 1 cut(s) | 374 |
| PspEI | GGTNACC | 1 cut(s) | 506 |
| PspFI | CCCAGC | 1 cut(s) | 433 |
| PspN4I | GGNNCC | 4 cut(s) | 120, 203, 330, 471 |
| PstI | CTGCAG | 1 cut(s) | 62 |
| PsuI | RGATCY | 1 cut(s) | 166 |
| RsaI | GTAC | 4 cut(s) | 30, 112, 126, 376 |
| RsaNI | GTAC | 4 cut(s) | 29, 111, 125, 375 |
| RseI | CAYNNNNRTG | 1 cut(s) | 364 |
| SaqAI | TTAA | 1 cut(s) | 306 |
| Sau3AI | GATC | 3 cut(s) | 166, 226, 265 |
| SbfI | CCTGCAGG | 1 cut(s) | 62 |
| SdaI | CCTGCAGG | 1 cut(s) | 62 |
| SetI | ASST | 9 cut(s) | 34, 124, 157, 237, 330, 376, 422, 432, 558 |
| SfaNI | GCATC | 1 cut(s) | 356 |
| SfcI | CTRYAG | 1 cut(s) | 58 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 364 |
| SnaBI | TACGTA | 1 cut(s) | 374 |
| SphI | GCATGC | 1 cut(s) | 94 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 62 |
| Sse9I | AATT | 4 cut(s) | 81, 285, 302, 439 |
| SsiI | CCGC | 2 cut(s) | 290, 512 |
| SspMI | CTAG | 2 cut(s) | 333, 567 |
| StyI | CCWWGG | 1 cut(s) | 162 |
| TaaI | ACNGT | 3 cut(s) | 28, 129, 274 |
| TaiI | ACGT | 2 cut(s) | 376, 422 |
| TasI | AATT | 4 cut(s) | 81, 285, 302, 439 |
| TatI | WGTACW | 2 cut(s) | 110, 124 |
| TfiI | GAWTC | 1 cut(s) | 185 |
| Tru1I | TTAA | 1 cut(s) | 306 |
| Tru9I | TTAA | 1 cut(s) | 306 |
| TseFI | GTSAC | 1 cut(s) | 73 |
| Tsp45I | GTSAC | 1 cut(s) | 73 |
| TspDTI | ATGAA | 1 cut(s) | 566 |
| XapI | RAATTY | 1 cut(s) | 439 |
| XceI | RCATGY | 1 cut(s) | 94 |
| XspI | CTAG | 2 cut(s) | 333, 567 |
| Zsp2I | ATGCAT | 1 cut(s) | 371 |