Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6A

Orthogroup ID

OG0018135

Physical Location & Seq

Genomic Coordinates Forward (+)

42074063 .. 42076607

Gene Structure 2545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6AG236000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1446 bp

ATGCCCAAAGATATGGATAACAATCTGCCCCCTTCTGTAGAGTCTTCAACCAATGGATCCGAGGAGCACAAGTCCAAGGATCTCTCAGTGAGTAGCGATTCGATTCGTCACCGTTTTCTGGAATTTTTTGCTTCCCGTGGTCACAAGGTTCTTCCTAGTGCTTCTCTGGTCCCAGATGATCCCACAGTTCTACAAACAATTGCTGGAATGCTTCAGTTCAAGCCTATATTCCTTGGCCAGGTTCAAAGACTAGTACCTCGTGCAACAACTTCTCAAAGGTGCATTCGCACTAATGATGCGGAGAATGTAGGTAGAACAGCTCGGCATCATACATTCTTCGAAATGCTTGGCAACTTCAGTTTCGGAGATTACTTCAAGAAGGAAGCAATCCAATGGGCATGGGAGCTTTCGACTGAAGAGTTTGGAATACCAGCTGACAGGTTGTGGGTTAGTGTCTTCGAAGATGATGATGAAGCTTTCGAAATCTGGCATCATGAGGTTGGTGTTCCTGTGGAGCATATCCAGAGAATGGGTGAAGAAGACAACTTTTGGACTAGTGGTGTTACCGGTCCATGCGGTCCATGCTCCGAGATTTATTATGATTTCCATCCTGAGAGGGGATATTTAGATGCAGACCTCAATGATGATACCAGATTTATTGAGTTTTACAATTTGGTTTTTATGGAGTTCAACAAAAAGGTTGATGGTTCGCTTGAGCCCTTGAAACAGAAGAACATAGATACCGGACTTGGCCTGGAGCGTGTGGCTCGAATCCTTCAGAAGGTCTCGAACAACTATGAAACTGACTTGATCTATCCAATTATAGAGAAGGCCTCAGAATTAGCAAAGGTATCATACAGCCAAGCAAATGAGCAGTTCAAACTGAATTTTAAGATTGTCGGAGATCATCTGCGTGCAATTGTGTATCTCATATCAGATGGCGTTGTTCCATCAAATACCAAAAGAGGGTATGTCCTTCGAATGCTTATCAGAAGGGCGGTTCGTGCAGGCAGGTCGCTTGGTCTAAAGGGAGATTGTCAGGGAAACCTTGGAGAAACAGTTCTACCGGTCATTGCAGGGAAAGTAATTGAGATGAGCAGCCTTATTGATCCGGATGTGAAAGATAAAGCAGGCCGAATTCTTGAGGTGTTGCAAAGGGAAGAACTTGTGTTTCTGAAGACATTGTGCAAAGGAGAGAAGAAGCTTCACCAAATGCTAGATGAGGCGTTATCGAGAGCGGAGAAAAGTGGGAGTATGCCTTGCTTGTCGGGCAAAGATGCTTTTGTGTTGAAAGATACATATGGATTTCCTGTTGAACAAACAAGAGAAGTGGCTAAAGAACATGGTGTAGGTATAGATATGCATGGCTTTGATACTGAAATGGAAAACCAAAGGCGTCAGTCTAAGGCTGCACACAAAACTGTTGAAGTTGTCGGATGCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000049 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004812 GO:0004813 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006082 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006412 GO:0006418 GO:0006419 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008033 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016020 GO:0016070 GO:0016597 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0031406 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034645 GO:0034660 GO:0036094 GO:0043038 GO:0043039 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

DHHA1 tRNA-synt_2c tRNA_SAD

Protein Analysis

481

Amino Acids

54.33

Weight (kDa)

5.56

Isoelectric Point (pI)

35.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_2c	PF01411	35 - 476	1.7e-162	tRNA synthetases class II (A)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0018135)

Species	Orthologous Gene IDs
pyrus_communis	pycom05g12680
rosa_chinensis	RchiOBHm_Chr6g0279761
rosa_laevigata	RLG00000013123
rosa_multiflora	Rmu_sc0001719.1_g000017
rosa_roxburghii	Rroxscaffold_7G00188690
rosa_rugosa	Rorug06G0127600
rosa_samantha	Rh6AG236000 Rh6BG240200 Rh6CG242300 Rh6DG233900
rosa_wichuraiana	Rw6G020570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1002
AccB7I	CCANNNNNTGG	1 cut(s)	529
AccBSI	CCGCTC	1 cut(s)	1238
AccIII	TCCGGA	1 cut(s)	1111
AciI	CCGC	4 cut(s)	299, 576, 998, 1238
AclWI	GGATC	5 cut(s)	51, 64, 87, 173, 1103
AcoI	YGGCCR	1 cut(s)	235
AcsI	RAATTY	3 cut(s)	122, 886, 1137
AcuI	CTGAAG	5 cut(s)	197, 340, 435, 761, 1196
AcyI	GRCGYC	1 cut(s)	1396
AfaI	GTAC	1 cut(s)	255
AfiI	CCNNNNNNNGG	6 cut(s)	118, 238, 529, 617, 781, 966
AgeI	ACCGGT	2 cut(s)	566, 1066
AhlI	ACTAGT	2 cut(s)	250, 554
AjnI	CCWGG	2 cut(s)	237, 753
AloI	GAACNNNNNNTCC	2 cut(s)	1044, 1076
AluBI	AGCT	5 cut(s)	320, 406, 434, 476, 1204
AluI	AGCT	5 cut(s)	320, 406, 434, 476, 1204
Alw21I	GWGCWC	1 cut(s)	69
Alw26I	GTCTC	1 cut(s)	790
AlwI	GGATC	5 cut(s)	51, 64, 87, 173, 1103
Aor13HI	TCCGGA	1 cut(s)	1111
AoxI	GGCC	4 cut(s)	235, 751, 831, 1132
ApeKI	GCWGC	2 cut(s)	1098, 1409
ApoI	RAATTY	3 cut(s)	122, 886, 1137
AsiGI	ACCGGT	2 cut(s)	566, 1066
Asp700I	GAANNNNTTC	1 cut(s)	1059
AspS9I	GGNCC	3 cut(s)	169, 569, 578
AsuHPI	GGTGA	3 cut(s)	101, 545, 1199
AsuII	TTCGAA	4 cut(s)	339, 459, 480, 979
AvaII	GGWCC	3 cut(s)	169, 569, 578
BalI	TGGCCA	1 cut(s)	237
BamHI	GGATCC	1 cut(s)	56
BanII	GRGCYC	1 cut(s)	720
BauI	CACGAG	1 cut(s)	258
BbsI	GAAGAC	4 cut(s)	36, 448, 546, 1184
Bbv12I	GWGCWC	1 cut(s)	69
BbvI	GCAGC	2 cut(s)	1110, 1396
BccI	CCATC	4 cut(s)	615, 698, 932, 958
BcgI	CGANNNNNNTGC	2 cut(s)	996, 1030
BciT130I	CCWGG	2 cut(s)	239, 755
BcoDI	GTCTC	1 cut(s)	790
BcuI	ACTAGT	2 cut(s)	250, 554
BfaI	CTAG	4 cut(s)	156, 251, 555, 1217
BfmI	CTRYAG	1 cut(s)	36
BfuAI	ACCTGC	1 cut(s)	1002
BisI	GCNGC	2 cut(s)	1099, 1410
BlsI	GCNGC	2 cut(s)	1100, 1411
Bme1390I	CCNGG	2 cut(s)	239, 755
Bme18I	GGWCC	3 cut(s)	169, 569, 578
BmgT120I	GGNCC	3 cut(s)	169, 569, 578
BmiI	GGNNCC	2 cut(s)	58, 171
BmrFI	CCNGG	2 cut(s)	239, 755
BmsI	GCATC	6 cut(s)	286, 334, 499, 619, 1267, 1427
BpiI	GAAGAC	4 cut(s)	36, 448, 546, 1184
BplI	GAGNNNNNCTC	2 cut(s)	818, 850
BpmI	CTGGAG	1 cut(s)	776
Bpu14I	TTCGAA	4 cut(s)	339, 459, 480, 979
BpuEI	CTTGAG	2 cut(s)	734, 1163
BsaBI	GATNNNNATC	2 cut(s)	21, 606
BsaHI	GRCGYC	1 cut(s)	1396
BsaI	GGTCTC	1 cut(s)	790
BsaJI	CCNNGG	5 cut(s)	60, 75, 136, 232, 1048
BsaWI	WCCGGW	4 cut(s)	566, 743, 1066, 1111
BsaXI	ACNNNNNCTCC	4 cut(s)	56, 86, 1044, 1074
Bsc4I	CCNNNNNNNGG	6 cut(s)	118, 238, 529, 617, 781, 966
Bse118I	RCCGGY	2 cut(s)	566, 1066
Bse3DI	GCAATG	1 cut(s)	1071
Bse8I	GATNNNNATC	2 cut(s)	21, 606
BseAI	TCCGGA	1 cut(s)	1111
BseBI	CCWGG	2 cut(s)	239, 755
BseDI	CCNNGG	5 cut(s)	60, 75, 136, 232, 1048
BseGI	GGATG	3 cut(s)	607, 1120, 1442
BseJI	GATNNNNATC	2 cut(s)	21, 606
BseLI	CCNNNNNNNGG	6 cut(s)	118, 238, 529, 617, 781, 966
BseMI	GCAATG	1 cut(s)	1071
BseMII	CTCAG	3 cut(s)	99, 603, 849
BseRI	GAGGAG	1 cut(s)	77
BseXI	GCAGC	2 cut(s)	1110, 1396
BsgI	GTGCAG	2 cut(s)	1026, 1395
BshFI	GGCC	4 cut(s)	237, 753, 833, 1134
BshTI	ACCGGT	2 cut(s)	566, 1066
BsiHKAI	GWGCWC	1 cut(s)	69
BsiSI	CCGG	4 cut(s)	567, 744, 1067, 1112
BslFI	GGGAC	1 cut(s)	155
BslI	CCNNNNNNNGG	6 cut(s)	118, 238, 529, 617, 781, 966
BsmAI	GTCTC	1 cut(s)	790
BsmFI	GGGAC	1 cut(s)	155
BsmI	GAATGC	3 cut(s)	213, 282, 987
BsnI	GGCC	4 cut(s)	237, 753, 833, 1134
Bso31I	GGTCTC	1 cut(s)	790
Bsp119I	TTCGAA	4 cut(s)	339, 459, 480, 979
Bsp1286I	GDGCHC	2 cut(s)	69, 720
Bsp13I	TCCGGA	1 cut(s)	1111
Bsp143I	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
BspACI	CCGC	4 cut(s)	299, 576, 998, 1238
BspANI	GGCC	4 cut(s)	237, 753, 833, 1134
BspCNI	CTCAG	3 cut(s)	98, 604, 848
BspEI	TCCGGA	1 cut(s)	1111
BspHI	TCATGA	1 cut(s)	493
BspLI	GGNNCC	2 cut(s)	58, 171
BspMI	ACCTGC	1 cut(s)	1002
BspPI	GGATC	5 cut(s)	51, 64, 87, 173, 1103
BspT104I	TTCGAA	4 cut(s)	339, 459, 480, 979
BspTNI	GGTCTC	1 cut(s)	790
BsrBI	CCGCTC	1 cut(s)	1238
BsrDI	GCAATG	1 cut(s)	1071
BsrFI	RCCGGY	2 cut(s)	566, 1066
BssAI	RCCGGY	2 cut(s)	566, 1066
BssECI	CCNNGG	5 cut(s)	60, 75, 136, 232, 1048
BssMI	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
BssNI	GRCGYC	1 cut(s)	1396
BssSI	CACGAG	1 cut(s)	258
BssT1I	CCWWGG	3 cut(s)	75, 232, 1048
Bst2BI	CACGAG	1 cut(s)	258
Bst2UI	CCWGG	2 cut(s)	239, 755
Bst4CI	ACNGT	4 cut(s)	113, 187, 1060, 1423
Bst6I	CTCTTC	1 cut(s)	411
BstACI	GRCGYC	1 cut(s)	1396
BstBI	TTCGAA	4 cut(s)	339, 459, 480, 979
BstC8I	GCNNGC	3 cut(s)	915, 1009, 1132
BstDEI	CTNAG	4 cut(s)	85, 612, 835, 1404
BstDSI	CCRYGG	1 cut(s)	136
BstENI	CCTNNNNNAGG	2 cut(s)	236, 779
BstF5I	GGATG	3 cut(s)	607, 1120, 1442
BstKTI	GATC	6 cut(s)	59, 82, 181, 813, 907, 1111
BstMAI	GTCTC	1 cut(s)	790
BstMBI	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
BstMWI	GCNNNNNNNGC	3 cut(s)	582, 1004, 1269
BstNI	CCWGG	2 cut(s)	239, 755
BstSCI	CCNGG	2 cut(s)	237, 753
BstSFI	CTRYAG	1 cut(s)	36
BstV1I	GCAGC	2 cut(s)	1110, 1396
BstV2I	GAAGAC	4 cut(s)	36, 448, 546, 1184
BstX2I	RGATCY	2 cut(s)	56, 79
BstXI	CCANNNNNNTGG	1 cut(s)	13
BstYI	RGATCY	2 cut(s)	56, 79
BsuRI	GGCC	4 cut(s)	237, 753, 833, 1134
BtgI	CCRYGG	1 cut(s)	136
BtsCI	GGATG	3 cut(s)	607, 1120, 1442
BtsIMutI	CAGTG	1 cut(s)	93
BveI	ACCTGC	1 cut(s)	1002
Cac8I	GCNNGC	3 cut(s)	915, 1009, 1132
CciI	TCATGA	1 cut(s)	493
Cfr10I	RCCGGY	2 cut(s)	566, 1066
Cfr13I	GGNCC	3 cut(s)	169, 569, 578
CseI	GACGC	1 cut(s)	1385
Csp6I	GTAC	1 cut(s)	254
CspAI	ACCGGT	2 cut(s)	566, 1066
CspCI	CAANNNNNGTGG	2 cut(s)	1311, 1346
CviAII	CATG	6 cut(s)	399, 494, 573, 582, 1343, 1364
CviQI	GTAC	1 cut(s)	254
DdeI	CTNAG	4 cut(s)	85, 612, 835, 1404
DpnI	GATC	6 cut(s)	58, 81, 180, 812, 906, 1110
DpnII	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
EaeI	YGGCCR	1 cut(s)	235
Eam1104I	CTCTTC	1 cut(s)	411
EarI	CTCTTC	1 cut(s)	411
Eco130I	CCWWGG	3 cut(s)	75, 232, 1048
Eco147I	AGGCCT	1 cut(s)	833
Eco24I	GRGCYC	1 cut(s)	720
Eco31I	GGTCTC	1 cut(s)	790
Eco47I	GGWCC	3 cut(s)	169, 569, 578
Eco57I	CTGAAG	5 cut(s)	197, 340, 435, 761, 1196
EcoNI	CCTNNNNNAGG	2 cut(s)	236, 779
EcoRI	GAATTC	1 cut(s)	1137
EcoRII	CCWGG	2 cut(s)	237, 753
EcoT14I	CCWWGG	3 cut(s)	75, 232, 1048
EcoT22I	ATGCAT	1 cut(s)	1365
EcoT38I	GRGCYC	1 cut(s)	720
ErhI	CCWWGG	3 cut(s)	75, 232, 1048
FaeI	CATG	6 cut(s)	402, 497, 576, 585, 1346, 1367
FaqI	GGGAC	1 cut(s)	155
FatI	CATG	6 cut(s)	398, 493, 572, 581, 1342, 1363
FauNDI	CATATG	1 cut(s)	1300
Fnu4HI	GCNGC	2 cut(s)	1099, 1410
FokI	GGATG	2 cut(s)	594, 1127
FriOI	GRGCYC	1 cut(s)	720
Fsp4HI	GCNGC	2 cut(s)	1099, 1410
FspBI	CTAG	4 cut(s)	156, 251, 555, 1217
GluI	GCNGC	2 cut(s)	1099, 1410
GsuI	CTGGAG	1 cut(s)	776
HaeIII	GGCC	4 cut(s)	237, 753, 833, 1134
HapII	CCGG	4 cut(s)	567, 744, 1067, 1112
HgaI	GACGC	1 cut(s)	1385
Hin1I	GRCGYC	1 cut(s)	1396
Hin1II	CATG	6 cut(s)	402, 497, 576, 585, 1346, 1367
HindIII	AAGCTT	2 cut(s)	474, 1202
HinfI	GANTC	4 cut(s)	41, 98, 103, 771
HpaII	CCGG	4 cut(s)	567, 744, 1067, 1112
HphI	GGTGA	3 cut(s)	101, 545, 1199
Hpy188III	TCNNGA	9 cut(s)	119, 376, 494, 523, 611, 787, 1112, 1142, 1233
HpyAV	CCTTC	7 cut(s)	42, 373, 775, 785, 823, 986, 987
HpyCH4III	ACNGT	4 cut(s)	113, 187, 1060, 1423
HpyF10VI	GCNNNNNNNGC	3 cut(s)	582, 1004, 1269
HpyF3I	CTNAG	4 cut(s)	85, 612, 835, 1404
Hsp92I	GRCGYC	1 cut(s)	1396
Hsp92II	CATG	6 cut(s)	402, 497, 576, 585, 1346, 1367
Kpn2I	TCCGGA	1 cut(s)	1111
Kzo9I	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
LmnI	GCTCC	5 cut(s)	64, 403, 514, 590, 757
Lsp1109I	GCAGC	2 cut(s)	1110, 1396
LweI	GCATC	6 cut(s)	286, 334, 499, 619, 1267, 1427
MaeI	CTAG	4 cut(s)	156, 251, 555, 1217
MaeIII	GTNAC	3 cut(s)	107, 140, 562
MalI	GATC	6 cut(s)	58, 81, 180, 812, 906, 1110
MbiI	CCGCTC	1 cut(s)	1238
MboI	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
MfeI	CAATTG	3 cut(s)	198, 918, 1441
MflI	RGATCY	2 cut(s)	56, 79
MhlI	GDGCHC	2 cut(s)	69, 720
MlsI	TGGCCA	1 cut(s)	237
MluNI	TGGCCA	1 cut(s)	237
MlyI	GAGTC	1 cut(s)	50
MmeI	TCCRAC	2 cut(s)	880, 1414
MnlI	CCTC	9 cut(s)	55, 267, 490, 609, 647, 844, 959, 1138, 1216
Mox20I	TGGCCA	1 cut(s)	237
Mph1103I	ATGCAT	1 cut(s)	1365
MroI	TCCGGA	1 cut(s)	1111
MroXI	GAANNNNTTC	1 cut(s)	1059
MscI	TGGCCA	1 cut(s)	237
MseI	TTAA	1 cut(s)	891
MslI	CAYNNNNRTG	1 cut(s)	912
Msp20I	TGGCCA	1 cut(s)	237
MspA1I	CMGCKG	1 cut(s)	434
MspI	CCGG	4 cut(s)	567, 744, 1067, 1112
MspR9I	CCNGG	2 cut(s)	239, 755
MunI	CAATTG	3 cut(s)	198, 918, 1441
Mva1269I	GAATGC	3 cut(s)	213, 282, 987
MvaI	CCWGG	2 cut(s)	239, 755
MwoI	GCNNNNNNNGC	3 cut(s)	582, 1004, 1269
NdeI	CATATG	1 cut(s)	1300
NdeII	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
NlaIII	CATG	6 cut(s)	402, 497, 576, 585, 1346, 1367
NlaIV	GGNNCC	2 cut(s)	58, 171
NmeAIII	GCCGAG	1 cut(s)	301
NmuCI	GTSAC	2 cut(s)	107, 140
NsiI	ATGCAT	1 cut(s)	1365
NspV	TTCGAA	4 cut(s)	339, 459, 480, 979
PagI	TCATGA	1 cut(s)	493
PceI	AGGCCT	1 cut(s)	833
PctI	GAATGC	3 cut(s)	213, 282, 987
PdmI	GAANNNNTTC	1 cut(s)	1059
PfeI	GAWTC	3 cut(s)	98, 103, 771
PflMI	CCANNNNNTGG	1 cut(s)	529
PinAI	ACCGGT	2 cut(s)	566, 1066
PkrI	GCNGC	2 cut(s)	1100, 1411
PleI	GAGTC	1 cut(s)	49
PpsI	GAGTC	1 cut(s)	49
Psp6I	CCWGG	2 cut(s)	237, 753
PspGI	CCWGG	2 cut(s)	237, 753
PspN4I	GGNNCC	2 cut(s)	58, 171
PspPI	GGNCC	3 cut(s)	169, 569, 578
PsuI	RGATCY	2 cut(s)	56, 79
PvuII	CAGCTG	1 cut(s)	434
RsaI	GTAC	1 cut(s)	255
RsaNI	GTAC	1 cut(s)	254
RseI	CAYNNNNRTG	1 cut(s)	912
SaqAI	TTAA	1 cut(s)	891
SatI	GCNGC	2 cut(s)	1099, 1410
Sau3AI	GATC	6 cut(s)	56, 79, 178, 810, 904, 1108
Sau96I	GGNCC	3 cut(s)	169, 569, 578
SchI	GAGTC	1 cut(s)	50
ScrFI	CCNGG	2 cut(s)	239, 755
SduI	GDGCHC	2 cut(s)	69, 720
SfaNI	GCATC	6 cut(s)	286, 334, 499, 619, 1267, 1427
SfcI	CTRYAG	1 cut(s)	36
SfuI	TTCGAA	4 cut(s)	339, 459, 480, 979
SinI	GGWCC	3 cut(s)	169, 569, 578
SmiMI	CAYNNNNRTG	1 cut(s)	912
SmlI	CTYRAG	2 cut(s)	713, 1142
SmoI	CTYRAG	2 cut(s)	713, 1142
SpeI	ACTAGT	2 cut(s)	250, 554
SseBI	AGGCCT	1 cut(s)	833
SsiI	CCGC	4 cut(s)	299, 576, 998, 1238
SspMI	CTAG	4 cut(s)	156, 251, 555, 1217
StuI	AGGCCT	1 cut(s)	833
StyD4I	CCNGG	2 cut(s)	237, 753
StyI	CCWWGG	3 cut(s)	75, 232, 1048
TaaI	ACNGT	4 cut(s)	113, 187, 1060, 1423
TaqI	TCGA	9 cut(s)	101, 339, 410, 459, 480, 769, 788, 979, 1232
TfiI	GAWTC	3 cut(s)	98, 103, 771
Tru1I	TTAA	1 cut(s)	891
Tru9I	TTAA	1 cut(s)	891
TscAI	CASTG	1 cut(s)	93
TseFI	GTSAC	2 cut(s)	107, 140
TseI	GCWGC	2 cut(s)	1098, 1409
Tsp45I	GTSAC	2 cut(s)	107, 140
TspDTI	ATGAA	2 cut(s)	486, 813
TspRI	CASTG	1 cut(s)	93
Van91I	CCANNNNNTGG	1 cut(s)	529
VpaK11BI	GGWCC	3 cut(s)	169, 569, 578
XagI	CCTNNNNNAGG	2 cut(s)	236, 779
XapI	RAATTY	3 cut(s)	122, 886, 1137
XmnI	GAANNNNTTC	1 cut(s)	1059
XspI	CTAG	4 cut(s)	156, 251, 555, 1217
Zsp2I	ATGCAT	1 cut(s)	1365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.