Rh6CG242300

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0018135
Physical Location & Seq
Forward (+)
40857627 .. 40858226
600 bp
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UTR
Exon/CDS
Intron
Rh6CG242300.1

Sequence Viewer

Length: 600 bp
ATGGCCAAAGATAAGGATAACAATCTGCCCCCTTCTGTAGAGTCTTCAACCAATAATGGATCCGAGGAGCACAAGTCCAAGGATCTCTCAGTGAGTAGCGATTCGATTCGTCACCGTTTTCTGGAATTTTTTGCTTCCCGTGGTCACAAGGTTCTTCCTAGTGCTTCTCTGGTCCCAGATGATCCCACAGTTCTACTAACAATTGCTGGAATGCTTCAGTTCAAGCCTATATTCCTTGGCCAGGTTCAAAGACTAGTACCTCGTGGAACAACTTCTCAAAGGTGCATTCGCACTAATGATGTGGAGAATGTAGGTAGAACAGCTCGGCATCATACATTCTTCGAAATGCTTGGCAACTTCAGTTTCGGAGATTACTTCAAGAAGGAAGCAATCCAATGGGCATGGGAGCTTTCGACTGAAGAGTTTGGAATACCAGCTGACAGGTTGCGGGTTAGTGTCTTCGAAGATGATGATGAAGCTTTCGAAATCTGGCATCATGAGGTTGGTGTTCCTGTGGAGCATATCCAGAGAATGGGTGAAGAAGACAACTTTTGGACTAGTGGAGTTACCGGTCCATGCGGTCCATGCTCCGAGATTTAA

Protein Analysis

199

Amino Acids

22.48

Weight (kDa)

5.14

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2c PF01411 36 - 199 8.4e-79 tRNA synthetases class II (A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018135)

Species Orthologous Gene IDs
pyrus_communis pycom05g12680
rosa_chinensis RchiOBHm_Chr6g0279761
rosa_laevigata RLG00000013123
rosa_multiflora Rmu_sc0001719.1_g000017
rosa_roxburghii Rroxscaffold_7G00188690
rosa_rugosa Rorug06G0127600
rosa_samantha Rh6AG236000 Rh6BG240200 Rh6CG242300 Rh6DG233900
rosa_wichuraiana Rw6G020570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 532
AciI CCGC 2 cut(s) 448, 579
AclWI GGATC 4 cut(s) 54, 67, 90, 176
AcoI YGGCCR 2 cut(s) 3, 238
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 3 cut(s) 200, 343, 438
AfaI GTAC 1 cut(s) 258
AfiI CCNNNNNNNGG 3 cut(s) 121, 241, 532
AgeI ACCGGT 1 cut(s) 569
AgsI TTSAA 4 cut(s) 48, 223, 248, 379
AhlI ACTAGT 2 cut(s) 253, 557
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 4 cut(s) 323, 409, 437, 479
AluI AGCT 4 cut(s) 323, 409, 437, 479
Alw21I GWGCWC 1 cut(s) 72
AlwI GGATC 4 cut(s) 54, 67, 90, 176
AoxI GGCC 2 cut(s) 3, 238
ApoI RAATTY 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 271
AspS9I GGNCC 3 cut(s) 172, 572, 581
AsuHPI GGTGA 2 cut(s) 104, 548
AsuII TTCGAA 3 cut(s) 342, 462, 483
AvaII GGWCC 3 cut(s) 172, 572, 581
BalI TGGCCA 2 cut(s) 5, 240
BamHI GGATCC 1 cut(s) 59
BauI CACGAG 1 cut(s) 261
BbsI GAAGAC 3 cut(s) 36, 451, 549
Bbv12I GWGCWC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 242
BcuI ACTAGT 2 cut(s) 253, 557
BfaI CTAG 3 cut(s) 159, 254, 558
BfmI CTRYAG 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 3 cut(s) 172, 572, 581
BmgT120I GGNCC 3 cut(s) 172, 572, 581
BmiI GGNNCC 2 cut(s) 61, 174
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 337, 502
BpiI GAAGAC 3 cut(s) 36, 451, 549
Bpu14I TTCGAA 3 cut(s) 342, 462, 483
BsaBI GATNNNNATC 1 cut(s) 21
BsaJI CCNNGG 4 cut(s) 63, 78, 139, 235
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 241, 532
Bse118I RCCGGY 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 4 cut(s) 63, 78, 139, 235
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 3 cut(s) 121, 241, 532
BseMII CTCAG 1 cut(s) 102
BseRI GAGGAG 1 cut(s) 80
BshFI GGCC 2 cut(s) 5, 240
BshTI ACCGGT 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 3 cut(s) 121, 241, 532
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 2 cut(s) 216, 285
BsnI GGCC 2 cut(s) 5, 240
Bsp119I TTCGAA 3 cut(s) 342, 462, 483
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 59, 82, 181
BspACI CCGC 2 cut(s) 448, 579
BspANI GGCC 2 cut(s) 5, 240
BspCNI CTCAG 1 cut(s) 101
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 61, 174
BspPI GGATC 4 cut(s) 54, 67, 90, 176
BspT104I TTCGAA 3 cut(s) 342, 462, 483
BsrFI RCCGGY 1 cut(s) 569
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 4 cut(s) 63, 78, 139, 235
BssMI GATC 3 cut(s) 59, 82, 181
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 2 cut(s) 78, 235
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 116, 190
Bst6I CTCTTC 1 cut(s) 414
BstBI TTCGAA 3 cut(s) 342, 462, 483
BstDEI CTNAG 1 cut(s) 88
BstDSI CCRYGG 1 cut(s) 139
BstENI CCTNNNNNAGG 1 cut(s) 239
BstKTI GATC 3 cut(s) 62, 85, 184
BstMBI GATC 3 cut(s) 59, 82, 181
BstMWI GCNNNNNNNGC 1 cut(s) 585
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 36
BstV2I GAAGAC 3 cut(s) 36, 451, 549
BstX2I RGATCY 2 cut(s) 59, 82
BstYI RGATCY 2 cut(s) 59, 82
BsuRI GGCC 2 cut(s) 5, 240
BtgI CCRYGG 1 cut(s) 139
BtsIMutI CAGTG 1 cut(s) 96
CciI TCATGA 1 cut(s) 496
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 3 cut(s) 172, 572, 581
Csp6I GTAC 1 cut(s) 257
CspAI ACCGGT 1 cut(s) 569
CviAII CATG 4 cut(s) 402, 497, 576, 585
CviJI RGCY 7 cut(s) 5, 226, 240, 323, 409, 437, 479
CviKI_1 RGCY 7 cut(s) 5, 226, 240, 323, 409, 437, 479
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 1 cut(s) 88
DpnI GATC 3 cut(s) 61, 84, 183
DpnII GATC 3 cut(s) 59, 82, 181
EaeI YGGCCR 2 cut(s) 3, 238
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 78, 235
Eco47I GGWCC 3 cut(s) 172, 572, 581
Eco57I CTGAAG 3 cut(s) 200, 343, 438
EcoNI CCTNNNNNAGG 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 240
EcoT14I CCWWGG 2 cut(s) 78, 235
ErhI CCWWGG 2 cut(s) 78, 235
FaeI CATG 4 cut(s) 405, 500, 579, 588
FaiI YATR 7 cut(s) 230, 333, 403, 498, 522, 577, 586
FaqI GGGAC 1 cut(s) 158
FatI CATG 4 cut(s) 401, 496, 575, 584
FauI CCCGC 1 cut(s) 441
FspBI CTAG 3 cut(s) 159, 254, 558
HaeIII GGCC 2 cut(s) 5, 240
HapII CCGG 1 cut(s) 570
Hin1II CATG 4 cut(s) 405, 500, 579, 588
HindIII AAGCTT 1 cut(s) 477
HinfI GANTC 3 cut(s) 41, 101, 106
HpaII CCGG 1 cut(s) 570
HphI GGTGA 2 cut(s) 104, 548
Hpy188I TCNGA 3 cut(s) 64, 368, 592
Hpy188III TCNNGA 4 cut(s) 122, 379, 497, 526
HpyAV CCTTC 2 cut(s) 42, 376
HpyCH4III ACNGT 2 cut(s) 116, 190
HpyCH4V TGCA 1 cut(s) 285
HpyF10VI GCNNNNNNNGC 1 cut(s) 585
HpyF3I CTNAG 1 cut(s) 88
Hsp92II CATG 4 cut(s) 405, 500, 579, 588
Kzo9I GATC 3 cut(s) 59, 82, 181
LmnI GCTCC 4 cut(s) 67, 406, 517, 593
LweI GCATC 2 cut(s) 337, 502
MaeI CTAG 3 cut(s) 159, 254, 558
MaeIII GTNAC 3 cut(s) 110, 143, 565
MalI GATC 3 cut(s) 61, 84, 183
MboI GATC 3 cut(s) 59, 82, 181
MboII GAAGA 8 cut(s) 36, 146, 331, 431, 451, 476, 551, 554
MfeI CAATTG 1 cut(s) 201
MflI RGATCY 2 cut(s) 59, 82
MhlI GDGCHC 1 cut(s) 72
MlsI TGGCCA 2 cut(s) 5, 240
MluCI AATT 2 cut(s) 125, 201
MluNI TGGCCA 2 cut(s) 5, 240
MlyI GAGTC 1 cut(s) 50
MnlI CCTC 3 cut(s) 58, 270, 493
Mox20I TGGCCA 2 cut(s) 5, 240
MroXI GAANNNNTTC 1 cut(s) 271
MscI TGGCCA 2 cut(s) 5, 240
MseI TTAA 1 cut(s) 598
Msp20I TGGCCA 2 cut(s) 5, 240
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 1 cut(s) 570
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 201
Mva1269I GAATGC 2 cut(s) 216, 285
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 585
NdeII GATC 3 cut(s) 59, 82, 181
NlaIII CATG 4 cut(s) 405, 500, 579, 588
NlaIV GGNNCC 2 cut(s) 61, 174
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 110, 143
NspV TTCGAA 3 cut(s) 342, 462, 483
PagI TCATGA 1 cut(s) 496
PctI GAATGC 2 cut(s) 216, 285
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 101, 106
PflMI CCANNNNNTGG 1 cut(s) 532
PinAI ACCGGT 1 cut(s) 569
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 2 cut(s) 61, 174
PspPI GGNCC 3 cut(s) 172, 572, 581
PsuI RGATCY 2 cut(s) 59, 82
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 598
Sau3AI GATC 3 cut(s) 59, 82, 181
Sau96I GGNCC 3 cut(s) 172, 572, 581
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 1 cut(s) 242
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 337, 502
SfcI CTRYAG 1 cut(s) 36
SfuI TTCGAA 3 cut(s) 342, 462, 483
SinI GGWCC 3 cut(s) 172, 572, 581
SpeI ACTAGT 2 cut(s) 253, 557
Sse9I AATT 2 cut(s) 125, 201
SsiI CCGC 2 cut(s) 448, 579
SspMI CTAG 3 cut(s) 159, 254, 558
StyD4I CCNGG 1 cut(s) 240
StyI CCWWGG 2 cut(s) 78, 235
TaaI ACNGT 2 cut(s) 116, 190
TaqI TCGA 5 cut(s) 104, 342, 413, 462, 483
TasI AATT 2 cut(s) 125, 201
TfiI GAWTC 2 cut(s) 101, 106
Tru1I TTAA 1 cut(s) 598
Tru9I TTAA 1 cut(s) 598
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 2 cut(s) 110, 143
Tsp45I GTSAC 2 cut(s) 110, 143
TspDTI ATGAA 1 cut(s) 489
TspRI CASTG 1 cut(s) 96
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 3 cut(s) 172, 572, 581
XagI CCTNNNNNAGG 1 cut(s) 239
XapI RAATTY 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 3 cut(s) 159, 254, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.