Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain
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| Species | Orthologous Gene IDs |
|---|---|
| pyrus_communis | pycom05g12680 |
| rosa_chinensis | RchiOBHm_Chr6g0279761 |
| rosa_laevigata | RLG00000013123 |
| rosa_multiflora | Rmu_sc0001719.1_g000017 |
| rosa_roxburghii | Rroxscaffold_7G00188690 |
| rosa_rugosa | Rorug06G0127600 |
| rosa_samantha | Rh6AG236000 Rh6BG240200 Rh6CG242300 Rh6DG233900 |
| rosa_wichuraiana | Rw6G020570 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 57 |
| AccB1I | GGYRCC | 1 cut(s) | 57 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 617 |
| AccII | CGCG | 1 cut(s) | 507 |
| AciI | CCGC | 1 cut(s) | 61 |
| AdeI | CACNNNGTG | 1 cut(s) | 530 |
| AfaI | GTAC | 2 cut(s) | 59, 377 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 54, 617 |
| AflIII | ACRYGT | 1 cut(s) | 395 |
| AgsI | TTSAA | 5 cut(s) | 9, 143, 288, 502, 562 |
| AjnI | CCWGG | 1 cut(s) | 665 |
| AluBI | AGCT | 4 cut(s) | 32, 67, 278, 511 |
| AluI | AGCT | 4 cut(s) | 32, 67, 278, 511 |
| Alw21I | GWGCWC | 1 cut(s) | 395 |
| Alw26I | GTCTC | 2 cut(s) | 177, 542 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 602 |
| ApeKI | GCWGC | 2 cut(s) | 642, 670 |
| Asp718I | GGTACC | 1 cut(s) | 57 |
| AsuHPI | GGTGA | 2 cut(s) | 320, 593 |
| BanI | GGYRCC | 1 cut(s) | 57 |
| BbsI | GAAGAC | 1 cut(s) | 44 |
| Bbv12I | GWGCWC | 1 cut(s) | 395 |
| BbvI | GCAGC | 2 cut(s) | 629, 682 |
| BccI | CCATC | 1 cut(s) | 611 |
| BceAI | ACGGC | 1 cut(s) | 513 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 662, 696 |
| BciT130I | CCWGG | 1 cut(s) | 667 |
| BcoDI | GTCTC | 2 cut(s) | 177, 542 |
| BfaI | CTAG | 1 cut(s) | 98 |
| BisI | GCNGC | 2 cut(s) | 643, 671 |
| BlsI | GCNGC | 2 cut(s) | 644, 672 |
| Bme1390I | CCNGG | 1 cut(s) | 667 |
| BmiI | GGNNCC | 1 cut(s) | 59 |
| BmrFI | CCNGG | 1 cut(s) | 667 |
| BpiI | GAAGAC | 1 cut(s) | 44 |
| Bpu10I | CCTNAGC | 2 cut(s) | 279, 301 |
| BsaJI | CCNNGG | 1 cut(s) | 435 |
| BsaWI | WCCGGW | 1 cut(s) | 263 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 54, 617 |
| Bse3DI | GCAATG | 1 cut(s) | 660 |
| BseBI | CCWGG | 1 cut(s) | 667 |
| BseDI | CCNNGG | 1 cut(s) | 435 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 54, 617 |
| BseMI | GCAATG | 1 cut(s) | 660 |
| BseMII | CTCAG | 3 cut(s) | 24, 288, 315 |
| BseRI | GAGGAG | 1 cut(s) | 647 |
| BseXI | GCAGC | 2 cut(s) | 629, 682 |
| Bsh1236I | CGCG | 1 cut(s) | 507 |
| BshNI | GGYRCC | 1 cut(s) | 57 |
| BsiHKAI | GWGCWC | 1 cut(s) | 395 |
| BsiSI | CCGG | 1 cut(s) | 264 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 54, 617 |
| BsmAI | GTCTC | 2 cut(s) | 177, 542 |
| BsmBI | CGTCTC | 1 cut(s) | 542 |
| Bsp1286I | GDGCHC | 1 cut(s) | 395 |
| BspACI | CCGC | 1 cut(s) | 61 |
| BspCNI | CTCAG | 3 cut(s) | 25, 287, 314 |
| BspFNI | CGCG | 1 cut(s) | 507 |
| BspLI | GGNNCC | 1 cut(s) | 59 |
| BspT107I | GGYRCC | 1 cut(s) | 57 |
| BsrDI | GCAATG | 1 cut(s) | 660 |
| BssECI | CCNNGG | 1 cut(s) | 435 |
| BssT1I | CCWWGG | 1 cut(s) | 435 |
| Bst2UI | CCWGG | 1 cut(s) | 667 |
| Bst4CI | ACNGT | 3 cut(s) | 338, 404, 604 |
| Bst6I | CTCTTC | 1 cut(s) | 144 |
| BstC8I | GCNNGC | 2 cut(s) | 65, 509 |
| BstDEI | CTNAG | 5 cut(s) | 33, 68, 274, 279, 301 |
| BstFNI | CGCG | 1 cut(s) | 507 |
| BstMAI | GTCTC | 2 cut(s) | 177, 542 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 297, 513, 662 |
| BstNI | CCWGG | 1 cut(s) | 667 |
| BstNSI | RCATGY | 1 cut(s) | 399 |
| BstSCI | CCNGG | 1 cut(s) | 665 |
| BstUI | CGCG | 1 cut(s) | 507 |
| BstV1I | GCAGC | 2 cut(s) | 629, 682 |
| BstV2I | GAAGAC | 1 cut(s) | 44 |
| BtsIMutI | CAGTG | 1 cut(s) | 343 |
| Cac8I | GCNNGC | 2 cut(s) | 65, 509 |
| CaiI | CAGNNNCTG | 1 cut(s) | 602 |
| Csp6I | GTAC | 2 cut(s) | 58, 376 |
| CviAII | CATG | 3 cut(s) | 199, 396, 622 |
| CviJI | RGCY | 8 cut(s) | 32, 67, 171, 195, 278, 300, 511, 656 |
| CviKI_1 | RGCY | 8 cut(s) | 32, 67, 171, 195, 278, 300, 511, 656 |
| CviQI | GTAC | 2 cut(s) | 58, 376 |
| DdeI | CTNAG | 5 cut(s) | 33, 68, 274, 279, 301 |
| DraIII | CACNNNGTG | 1 cut(s) | 530 |
| Eam1104I | CTCTTC | 1 cut(s) | 144 |
| EarI | CTCTTC | 1 cut(s) | 144 |
| Eco130I | CCWWGG | 1 cut(s) | 435 |
| EcoRII | CCWGG | 1 cut(s) | 665 |
| EcoT14I | CCWWGG | 1 cut(s) | 435 |
| ErhI | CCWWGG | 1 cut(s) | 435 |
| Esp3I | CGTCTC | 1 cut(s) | 542 |
| FaeI | CATG | 3 cut(s) | 202, 399, 625 |
| FatI | CATG | 3 cut(s) | 198, 395, 621 |
| Fnu4HI | GCNGC | 2 cut(s) | 643, 671 |
| Fsp4HI | GCNGC | 2 cut(s) | 643, 671 |
| FspBI | CTAG | 1 cut(s) | 98 |
| GluI | GCNGC | 2 cut(s) | 643, 671 |
| HapII | CCGG | 1 cut(s) | 264 |
| Hin1II | CATG | 3 cut(s) | 202, 399, 625 |
| HinfI | GANTC | 6 cut(s) | 5, 284, 346, 355, 460, 484 |
| HpaII | CCGG | 1 cut(s) | 264 |
| HphI | GGTGA | 2 cut(s) | 320, 593 |
| Hpy188I | TCNGA | 7 cut(s) | 182, 333, 351, 390, 469, 493, 547 |
| Hpy188III | TCNNGA | 5 cut(s) | 121, 129, 160, 303, 562 |
| Hpy99I | CGWCG | 1 cut(s) | 227 |
| HpyCH4III | ACNGT | 3 cut(s) | 338, 404, 604 |
| HpyCH4V | TGCA | 3 cut(s) | 421, 645, 688 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 297, 513, 662 |
| HpyF3I | CTNAG | 5 cut(s) | 33, 68, 274, 279, 301 |
| Hsp92II | CATG | 3 cut(s) | 202, 399, 625 |
| KpnI | GGTACC | 1 cut(s) | 61 |
| LmnI | GCTCC | 1 cut(s) | 123 |
| Lsp1109I | GCAGC | 2 cut(s) | 629, 682 |
| MaeI | CTAG | 1 cut(s) | 98 |
| MaeIII | GTNAC | 2 cut(s) | 93, 398 |
| MboII | GAAGA | 7 cut(s) | 49, 131, 364, 457, 598, 626, 704 |
| MhlI | GDGCHC | 1 cut(s) | 395 |
| MluCI | AATT | 1 cut(s) | 13 |
| MlyI | GAGTC | 2 cut(s) | 469, 493 |
| MmeI | TCCRAC | 1 cut(s) | 208 |
| MnlI | CCTC | 6 cut(s) | 48, 219, 299, 531, 625, 646 |
| MspI | CCGG | 1 cut(s) | 264 |
| MspR9I | CCNGG | 1 cut(s) | 667 |
| MvaI | CCWGG | 1 cut(s) | 667 |
| MvnI | CGCG | 1 cut(s) | 507 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 297, 513, 662 |
| NlaIII | CATG | 3 cut(s) | 202, 399, 625 |
| NlaIV | GGNNCC | 1 cut(s) | 59 |
| NmeAIII | GCCGAG | 1 cut(s) | 386 |
| NmuCI | GTSAC | 1 cut(s) | 398 |
| NspI | RCATGY | 1 cut(s) | 399 |
| PciI | ACATGT | 1 cut(s) | 395 |
| PfeI | GAWTC | 4 cut(s) | 5, 284, 346, 355 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 617 |
| PkrI | GCNGC | 2 cut(s) | 644, 672 |
| PleI | GAGTC | 2 cut(s) | 468, 492 |
| PpsI | GAGTC | 2 cut(s) | 468, 492 |
| PscI | ACATGT | 1 cut(s) | 395 |
| Psp6I | CCWGG | 1 cut(s) | 665 |
| PspGI | CCWGG | 1 cut(s) | 665 |
| PspN4I | GGNNCC | 1 cut(s) | 59 |
| PstNI | CAGNNNCTG | 1 cut(s) | 602 |
| RsaI | GTAC | 2 cut(s) | 59, 377 |
| RsaNI | GTAC | 2 cut(s) | 58, 376 |
| SatI | GCNGC | 2 cut(s) | 643, 671 |
| SchI | GAGTC | 2 cut(s) | 469, 493 |
| ScrFI | CCNGG | 1 cut(s) | 667 |
| SduI | GDGCHC | 1 cut(s) | 395 |
| Sse9I | AATT | 1 cut(s) | 13 |
| SsiI | CCGC | 1 cut(s) | 61 |
| SspMI | CTAG | 1 cut(s) | 98 |
| StyD4I | CCNGG | 1 cut(s) | 665 |
| StyI | CCWWGG | 1 cut(s) | 435 |
| TaaI | ACNGT | 3 cut(s) | 338, 404, 604 |
| TaqI | TCGA | 2 cut(s) | 372, 682 |
| TasI | AATT | 1 cut(s) | 13 |
| TatI | WGTACW | 1 cut(s) | 375 |
| TfiI | GAWTC | 4 cut(s) | 5, 284, 346, 355 |
| TscAI | CASTG | 1 cut(s) | 343 |
| TseFI | GTSAC | 1 cut(s) | 398 |
| TseI | GCWGC | 2 cut(s) | 642, 670 |
| Tsp45I | GTSAC | 1 cut(s) | 398 |
| TspDTI | ATGAA | 3 cut(s) | 187, 381, 649 |
| TspGWI | ACGGA | 1 cut(s) | 566 |
| TspRI | CASTG | 1 cut(s) | 343 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 617 |
| XceI | RCATGY | 1 cut(s) | 399 |
| XspI | CTAG | 1 cut(s) | 98 |