pycom05g19850

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003776
Physical Location & Seq
Reverse (-)
22730189 .. 22736052
5864 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1866 bp
ATGTCCAGCAATGGCCGCAAGTACTCGTGCGAAACGGCGAGCCAGACCCTGGAATGGATCAACGCCATCGTCGACTTCGTAAGACCCTACACTTTCCTAACCAACGCCCACGTCGTCAATTTCTTCAAGGACCGGCTGTGGGAAGCTGTTGACGAGGAATGGATGGATTGCTTGCGCAAAGAAGCAGTGGAGAATCTCCTGCAGATTCCTTGTGGAGTGATCCAGGATCATTGGCCTGCTTCGCTTAAGAATTTTATTGTTACTTTGAGCTCTCTTGTGCTTCCTCGACAACAAGCAGACTTGCAGACGGTATTACCTAGCATGGACATGACTTCGCTCAATAGCGTTCTCTCCACAGGCATGAATGCAAAGAAAAAACATGAAGTAGAAGTCCTTTCTGCGGTTGTTAATTCGATTGCTATCAGTGTCAGGGCTAATGCAATAATTGATGTTGGCTCTGGTCAGGGGTATCTTGCACAAGTACTTTCCTTTCACTACCAACATGCAGTATTTGCAATTGATGCATGTTCTCATCATGGAAGGGTTACTGATGCACGTGCAGAACAAATCAAGAAATATTATGCTTCTCAAATCCGTAAATCTGTATCAGGAAACAGGAGTTTGACTGTGCCTAAGACAATCACATGCAATGTGATGTCTATTGACACGTTGAAAGCTTTGGCTGACATACCACTGGATAAAGATAATATTTCTTCATTACAAACTTCCTGCAAGGGAGACAGTGGAACTTCACTGGTTCTTGCTGGCCTTCATGCTTGCGGAGATCTTTCAGTAACAATGCTCAAGACCTTTATGGAGTGCAAAGAAGTTAAAGCAGTTGTTAGCGTTGGCTGCTGTTACAACCTATTATCTGAAGAGGGTTCCAACCATGTGGGTTCTCAATGTGGTTTTCCAATGAGTCATGGTGTTAAATCTGCTGGTTTGTCACTTGGAAAAAGTTCGCGTGACCTTGCATGTCAGAGTGCGGAGAGGTGGAGATGCTTGGAAAATGATGCTGGCGTCCATAACTTTGAGCTGCATGCTTTCCGTGCTGCTTTTCAGATGGTTCTCAGTGTATATTATCCCAAAGTGATGACTACAAGTCCATCAATTGGGCGTCAAGGAAAGGCTTTGCGTCGCCGACAGCAGAGGGTCACCCCAGACTCCTCGCCGCATCATAAAGAAAACACATGTTCATCCTTGTCCCAAATAAACTCTGCTATGAAAGGAAGCTGGTCTCACATAAAACCCACAGTACAGGATGCAGAGACAACGACCATAACTAATGAAAATTCCTTCAACAAGGCCACCCAATGTGGGAGGAGCAAGTCCATCGGCAAGTATCCACTTTTCCAGAAATATTGCTTATCTGGTTTATCTCGCCTTGATCTTGAGCCTCTGAAGGAAATGGATGTTCATGGAATATGGAAGGAAGTTGAATCATCTGCTGAACTTATTGGACCTTATTGGTCTCTTCGAGCTGCTTTCGGGCCTCTTTTAGAAACTTTCCTTTTGCTTGATAGATTGTTGTTCCTCCAAGAGCAAGGGAGTTCAATTGAAGCAGAAATGCAACCAATTTTTAATCCTGCATTGTCCCCAAGGAATCCAGAGGTCATTAAGAGCCCCGTGGTGAACGACGCTGGTGATGCCGTGGGTTTGATCATTACTGGTTCTACTGACAATCTTCTAAGTTTGTTTTCAGGAGGAGATCCAGATCCGACAACTTATGCAACGCAGTTGCAAGAACATGTCGATGCATCATCAGTACTGAAATTATTGCCAAAGGCAGAAGATGTGTTGAATTGTGCAGTCTCAGGAGAACTCAAGGACTTGCTTGTACTGGATGGATGTAATCAAATTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_32

Protein Analysis

622

Amino Acids

68.0

Weight (kDa)

6.12

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_32 PF13679 124 - 292 6.4e-26 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 176
AccB7I CCANNNNNTGG 2 cut(s) 49, 1112
AccI GTMKAC 1 cut(s) 72
AccII CGCG 1 cut(s) 964
AciI CCGC 5 cut(s) 16, 401, 780, 986, 1172
AclWI GGATC 5 cut(s) 65, 214, 234, 1703, 1709
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 2 cut(s) 250, 1291
AcuI CTGAAG 2 cut(s) 894, 1421
AcvI CACGTG 1 cut(s) 557
AcyI GRCGYC 2 cut(s) 1020, 1117
AfaI GTAC 5 cut(s) 23, 483, 1257, 1767, 1839
AfiI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1317
AflII CTTAAG 1 cut(s) 245
AflIII ACRYGT 3 cut(s) 666, 1190, 1747
AgsI TTSAA 7 cut(s) 127, 673, 1300, 1439, 1554, 1559, 1801
AhdI GACNNNNNGTC 1 cut(s) 1101
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 2 cut(s) 48, 222
AluBI AGCT 6 cut(s) 146, 270, 677, 1036, 1233, 1481
AluI AGCT 6 cut(s) 146, 270, 677, 1036, 1233, 1481
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 5 cut(s) 732, 1242, 1262, 1476, 1816
AlwI GGATC 5 cut(s) 65, 214, 234, 1703, 1709
AlwNI CAGNNNCTG 1 cut(s) 49
AoxI GGCC 5 cut(s) 13, 233, 766, 1305, 1490
ApeKI GCWGC 4 cut(s) 852, 1036, 1052, 1481
ApoI RAATTY 2 cut(s) 250, 1291
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 3 cut(s) 130, 1460, 1490
AsuHPI GGTGA 3 cut(s) 1147, 1642, 1655
AvaII GGWCC 2 cut(s) 130, 1460
BanII GRGCYC 2 cut(s) 272, 1625
BauI CACGAG 1 cut(s) 25
BbrPI CACGTG 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 4 cut(s) 839, 1023, 1039, 1468
BccI CCATC 6 cut(s) 74, 157, 1057, 1114, 1340, 1838
BceAI ACGGC 2 cut(s) 51, 1634
BcgI CGANNNNNNTGC 2 cut(s) 1315, 1349
BciT130I CCWGG 2 cut(s) 50, 224
BciVI GTATCC 1 cut(s) 1353
BclI TGATCA 1 cut(s) 1659
BcoDI GTCTC 5 cut(s) 732, 1242, 1262, 1476, 1816
BfaI CTAG 1 cut(s) 318
BfmI CTRYAG 1 cut(s) 200
BfrI CTTAAG 1 cut(s) 245
BfuI GTATCC 1 cut(s) 1353
BglII AGATCT 1 cut(s) 784
BisI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1482
BlsI GCNGC 6 cut(s) 17, 854, 1038, 1054, 1173, 1483
BmcAI AGTACT 3 cut(s) 23, 483, 1767
Bme1390I CCNGG 2 cut(s) 50, 224
Bme18I GGWCC 2 cut(s) 130, 1460
BmeRI GACNNNNNGTC 1 cut(s) 1101
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 3 cut(s) 130, 1460, 1490
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 2 cut(s) 50, 224
BmsI GCATC 9 cut(s) 511, 541, 989, 1003, 1183, 1252, 1636, 1744, 1766
BpuEI CTTGAG 3 cut(s) 788, 1412, 1808
BsaAI YACGTR 1 cut(s) 557
BsaBI GATNNNNATC 2 cut(s) 419, 1713
BsaHI GRCGYC 2 cut(s) 1020, 1117
BsaI GGTCTC 2 cut(s) 1242, 1476
BsaJI CCNNGG 4 cut(s) 48, 1598, 1626, 1650
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1317
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 4 cut(s) 699, 759, 1672, 1845
Bse3DI GCAATG 2 cut(s) 16, 655
Bse8I GATNNNNATC 2 cut(s) 419, 1713
BseBI CCWGG 2 cut(s) 50, 224
BseDI CCNNGG 4 cut(s) 48, 1598, 1626, 1650
BseGI GGATG 6 cut(s) 168, 1196, 1267, 1417, 1849, 1853
BseJI GATNNNNATC 2 cut(s) 419, 1713
BseLI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1317
BseMI GCAATG 2 cut(s) 16, 655
BseMII CTCAG 2 cut(s) 1084, 1827
BseNI ACTGG 4 cut(s) 699, 759, 1672, 1845
BseRI GAGGAG 3 cut(s) 1156, 1336, 1719
BseXI GCAGC 4 cut(s) 839, 1023, 1039, 1468
BsgI GTGCAG 2 cut(s) 579, 1827
Bsh1236I CGCG 1 cut(s) 964
BshFI GGCC 5 cut(s) 15, 235, 768, 1307, 1492
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 133
BslFI GGGAC 2 cut(s) 1189, 1579
BslI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1317
BsmAI GTCTC 5 cut(s) 732, 1242, 1262, 1476, 1816
BsmFI GGGAC 2 cut(s) 1189, 1579
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 5 cut(s) 15, 235, 768, 1307, 1492
Bso31I GGTCTC 2 cut(s) 1242, 1476
Bsp1286I GDGCHC 2 cut(s) 272, 1625
Bsp143I GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
BspACI CCGC 5 cut(s) 16, 401, 780, 986, 1172
BspANI GGCC 5 cut(s) 15, 235, 768, 1307, 1492
BspCNI CTCAG 2 cut(s) 1083, 1826
BspFNI CGCG 1 cut(s) 964
BspLI GGNNCC 1 cut(s) 883
BspMAI CTGCAG 1 cut(s) 204
BspPI GGATC 5 cut(s) 65, 214, 234, 1703, 1709
BspTI CTTAAG 1 cut(s) 245
BspTNI GGTCTC 2 cut(s) 1242, 1476
BsrDI GCAATG 2 cut(s) 16, 655
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 4 cut(s) 699, 759, 1672, 1845
BssAI RCCGGY 1 cut(s) 132
BssECI CCNNGG 4 cut(s) 48, 1598, 1626, 1650
BssMI GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
BssNI GRCGYC 2 cut(s) 1020, 1117
BssSI CACGAG 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 1598
Bst2BI CACGAG 1 cut(s) 25
Bst2UI CCWGG 2 cut(s) 50, 224
Bst4CI ACNGT 4 cut(s) 310, 628, 743, 1255
Bst6I CTCTTC 2 cut(s) 870, 1479
BstACI GRCGYC 2 cut(s) 1020, 1117
BstAFI CTTAAG 1 cut(s) 245
BstAPI GCANNNNNTGC 2 cut(s) 512, 521
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 7 cut(s) 40, 173, 237, 766, 778, 1018, 1041
BstDEI CTNAG 4 cut(s) 633, 1070, 1688, 1813
BstDSI CCRYGG 2 cut(s) 1626, 1650
BstEII GGTNACC 1 cut(s) 1153
BstF5I GGATG 6 cut(s) 168, 1196, 1267, 1417, 1849, 1853
BstFNI CGCG 1 cut(s) 964
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 8 cut(s) 60, 222, 229, 787, 1390, 1662, 1711, 1717
BstMAI GTCTC 5 cut(s) 732, 1242, 1262, 1476, 1816
BstMBI GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
BstMWI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1646
BstNI CCWGG 2 cut(s) 50, 224
BstNSI RCATGY 7 cut(s) 506, 528, 648, 978, 1043, 1194, 1751
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 2 cut(s) 48, 222
BstSFI CTRYAG 1 cut(s) 200
BstUI CGCG 1 cut(s) 964
BstV1I GCAGC 4 cut(s) 839, 1023, 1039, 1468
BstX2I RGATCY 3 cut(s) 784, 1708, 1714
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 3 cut(s) 784, 1708, 1714
BsuI GTATCC 1 cut(s) 1353
BsuRI GGCC 5 cut(s) 15, 235, 768, 1307, 1492
BtgI CCRYGG 2 cut(s) 1626, 1650
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 6 cut(s) 168, 1196, 1267, 1417, 1849, 1853
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 6 cut(s) 192, 430, 692, 748, 752, 1078
Cac8I GCNNGC 7 cut(s) 40, 173, 237, 766, 778, 1018, 1041
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 3 cut(s) 130, 1460, 1490
CseI GACGC 4 cut(s) 1009, 1106, 1124, 1646
Csp6I GTAC 5 cut(s) 22, 482, 1256, 1766, 1838
CviQI GTAC 5 cut(s) 22, 482, 1256, 1766, 1838
DdeI CTNAG 4 cut(s) 633, 1070, 1688, 1813
DpnI GATC 8 cut(s) 59, 221, 228, 786, 1389, 1661, 1710, 1716
DpnII GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
DriI GACNNNNNGTC 1 cut(s) 1101
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 870, 1479
Eam1105I GACNNNNNGTC 1 cut(s) 1101
EarI CTCTTC 2 cut(s) 870, 1479
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 1598
Eco24I GRGCYC 2 cut(s) 272, 1625
Eco31I GGTCTC 2 cut(s) 1242, 1476
Eco47I GGWCC 2 cut(s) 130, 1460
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 894, 1421
Eco72I CACGTG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 1153
EcoICRI GAGCTC 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 1153
EcoRII CCWGG 2 cut(s) 48, 222
EcoT14I CCWWGG 1 cut(s) 1598
EcoT22I ATGCAT 2 cut(s) 526, 1759
EcoT38I GRGCYC 2 cut(s) 272, 1625
ErhI CCWWGG 1 cut(s) 1598
FaqI GGGAC 2 cut(s) 1189, 1579
FbaI TGATCA 1 cut(s) 1659
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1482
FokI GGATG 6 cut(s) 175, 1183, 1274, 1424, 1856, 1860
FriOI GRGCYC 2 cut(s) 272, 1625
Fsp4HI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1482
FspBI CTAG 1 cut(s) 318
FspI TGCGCA 1 cut(s) 176
GlaI GCGC 1 cut(s) 176
GluI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1482
HaeIII GGCC 5 cut(s) 15, 235, 768, 1307, 1492
HapII CCGG 1 cut(s) 133
HgaI GACGC 4 cut(s) 1009, 1106, 1124, 1646
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 2 cut(s) 1020, 1117
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 2 cut(s) 73, 151
HindII GTYRAC 2 cut(s) 73, 151
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 6 cut(s) 193, 205, 919, 1163, 1439, 1603
HpaII CCGG 1 cut(s) 133
HphI GGTGA 3 cut(s) 1147, 1642, 1655
Hpy166II GTNNAC 3 cut(s) 73, 151, 1633
Hpy188I TCNGA 5 cut(s) 874, 981, 1062, 1401, 1719
Hpy188III TCNNGA 9 cut(s) 571, 609, 805, 1354, 1391, 1607, 1701, 1712, 1815
Hpy8I GTNNAC 3 cut(s) 73, 151, 1633
Hpy99I CGWCG 4 cut(s) 74, 116, 1140, 1640
HpyAV CCTTC 5 cut(s) 534, 779, 1306, 1396, 1423
HpyCH4III ACNGT 4 cut(s) 310, 628, 743, 1255
HpyCH4IV ACGT 3 cut(s) 111, 556, 668
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1646
HpyF3I CTNAG 4 cut(s) 633, 1070, 1688, 1813
HpySE526I ACGT 3 cut(s) 111, 556, 668
Hsp92I GRCGYC 2 cut(s) 1020, 1117
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 1659
Kzo9I GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
LmnI GCTCC 1 cut(s) 1323
Lsp1109I GCAGC 4 cut(s) 839, 1023, 1039, 1468
LweI GCATC 9 cut(s) 511, 541, 989, 1003, 1183, 1252, 1636, 1744, 1766
MaeI CTAG 1 cut(s) 318
MaeII ACGT 3 cut(s) 111, 556, 668
MaeIII GTNAC 7 cut(s) 259, 544, 793, 857, 945, 965, 1153
MalI GATC 8 cut(s) 59, 221, 228, 786, 1389, 1661, 1710, 1716
MboI GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
MboII GAAGA 6 cut(s) 115, 705, 887, 1466, 1676, 1802
MfeI CAATTG 3 cut(s) 516, 1110, 1554
MflI RGATCY 3 cut(s) 784, 1708, 1714
MhlI GDGCHC 2 cut(s) 272, 1625
MlyI GAGTC 2 cut(s) 928, 1157
MmeI TCCRAC 2 cut(s) 909, 1742
Mph1103I ATGCAT 2 cut(s) 526, 1759
MroXI GAANNNNTTC 1 cut(s) 958
MseI TTAA 7 cut(s) 246, 408, 831, 930, 1581, 1617, 1864
MslI CAYNNNNRTG 3 cut(s) 326, 359, 1752
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 2 cut(s) 50, 224
MunI CAATTG 3 cut(s) 516, 1110, 1554
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 2 cut(s) 50, 224
MvnI CGCG 1 cut(s) 964
MwoI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1646
NdeII GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
NlaIV GGNNCC 1 cut(s) 883
NmuCI GTSAC 3 cut(s) 945, 965, 1153
NsbI TGCGCA 1 cut(s) 176
NsiI ATGCAT 2 cut(s) 526, 1759
NspI RCATGY 7 cut(s) 506, 528, 648, 978, 1043, 1194, 1751
PaeI GCATGC 1 cut(s) 1043
PciI ACATGT 2 cut(s) 1190, 1747
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 75, 111
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 4 cut(s) 193, 205, 1439, 1603
PflMI CCANNNNNTGG 2 cut(s) 49, 1112
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 6 cut(s) 17, 854, 1038, 1054, 1173, 1483
PleI GAGTC 2 cut(s) 927, 1157
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
PpsI GAGTC 2 cut(s) 927, 1157
Ppu21I YACGTR 1 cut(s) 557
PscI ACATGT 2 cut(s) 1190, 1747
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 48, 222
PspCI CACGTG 1 cut(s) 557
PspEI GGTNACC 1 cut(s) 1153
PspGI CCWGG 2 cut(s) 48, 222
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 3 cut(s) 130, 1460, 1490
PstI CTGCAG 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 3 cut(s) 784, 1708, 1714
RsaI GTAC 5 cut(s) 23, 483, 1257, 1767, 1839
RsaNI GTAC 5 cut(s) 22, 482, 1256, 1766, 1838
RseI CAYNNNNRTG 3 cut(s) 326, 359, 1752
SacI GAGCTC 1 cut(s) 272
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 7 cut(s) 246, 408, 831, 930, 1581, 1617, 1864
SatI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1482
Sau3AI GATC 8 cut(s) 57, 219, 226, 784, 1387, 1659, 1708, 1714
Sau96I GGNCC 3 cut(s) 130, 1460, 1490
ScaI AGTACT 3 cut(s) 23, 483, 1767
SchI GAGTC 2 cut(s) 928, 1157
ScrFI CCNGG 2 cut(s) 50, 224
SduI GDGCHC 2 cut(s) 272, 1625
SfaNI GCATC 9 cut(s) 511, 541, 989, 1003, 1183, 1252, 1636, 1744, 1766
SfcI CTRYAG 1 cut(s) 200
SinI GGWCC 2 cut(s) 130, 1460
SmiMI CAYNNNNRTG 3 cut(s) 326, 359, 1752
SmlI CTYRAG 4 cut(s) 245, 803, 1391, 1823
SmoI CTYRAG 4 cut(s) 245, 803, 1391, 1823
SphI GCATGC 1 cut(s) 1043
SsiI CCGC 5 cut(s) 16, 401, 780, 986, 1172
SspI AATATT 3 cut(s) 578, 709, 1361
SspMI CTAG 1 cut(s) 318
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 2 cut(s) 48, 222
StyI CCWWGG 1 cut(s) 1598
TaaI ACNGT 4 cut(s) 310, 628, 743, 1255
TaiI ACGT 3 cut(s) 114, 559, 671
TaqI TCGA 5 cut(s) 72, 286, 413, 1477, 1752
TatI WGTACW 5 cut(s) 21, 481, 1255, 1765, 1837
TauI GCSGC 2 cut(s) 18, 1174
TfiI GAWTC 4 cut(s) 193, 205, 1439, 1603
Tru1I TTAA 7 cut(s) 246, 408, 831, 930, 1581, 1617, 1864
Tru9I TTAA 7 cut(s) 246, 408, 831, 930, 1581, 1617, 1864
TscAI CASTG 6 cut(s) 192, 430, 699, 748, 759, 1078
TseFI GTSAC 3 cut(s) 945, 965, 1153
TseI GCWGC 4 cut(s) 852, 1036, 1052, 1481
Tsp45I GTSAC 3 cut(s) 945, 965, 1153
TspDTI ATGAA 8 cut(s) 377, 396, 705, 761, 1185, 1238, 1302, 1406
TspGWI ACGGA 2 cut(s) 584, 1037
TspRI CASTG 6 cut(s) 192, 430, 699, 748, 759, 1078
Van91I CCANNNNNTGG 2 cut(s) 49, 1112
Vha464I CTTAAG 1 cut(s) 245
VpaK11BI GGWCC 2 cut(s) 130, 1460
XapI RAATTY 2 cut(s) 250, 1291
XceI RCATGY 7 cut(s) 506, 528, 648, 978, 1043, 1194, 1751
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 1 cut(s) 318
ZrmI AGTACT 3 cut(s) 23, 483, 1767
Zsp2I ATGCAT 2 cut(s) 526, 1759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.