pycom05g21270

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019752
Physical Location & Seq
Reverse (-)
23916682 .. 23918041
1360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21270.1

Sequence Viewer

Length: 411 bp
ATGACGTCGTTTAAGAAATTGGATCATCGGCAGAGCACTACGACACTTGTAGAGAGATGCAAAATGTTGGCTGCTAAGGTCGGAAAAATCCATAAAGCCAGAATTCCGCCTAGGACAGAAAACTCAACATGCTCACGTGGTGTGGAACCGCAATTCTTGGTTCTTCTGCTGGTTTTAAGGTCTTGTGGTGGTGATTTATTTACTTCTGACCTTCTTTTTCGATATTTTAAGGTTTATACAGAAAGAGTTTTTGTTTTAATTATATATTTACTACTTGGTAGTTCTGCAGACCATTGGCCGACGTCAGCAATGTCCAAAGCAACTCTTCTAGGATTGTTGGGGGTGATGCCTCTAAAGGAAAGTTGGTACCTTTTCATTTGCTCATATAGTTCCTTCATTTTTGGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.45

Weight (kDa)

9.57

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019752)

Species Orthologous Gene IDs
malus_domestica MD05G1236000.v1.1 MD14G1179200.v1.1 MD14G1179300.v1.1
pyrus_communis pycom05g21270 pycom05g21310 pycom05g23790 pycom05g24070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 305
Acc65I GGTACC 1 cut(s) 366
AccB1I GGYRCC 1 cut(s) 366
AciI CCGC 2 cut(s) 107, 149
AclWI GGATC 1 cut(s) 30
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 1 cut(s) 102
AcvI CACGTG 1 cut(s) 137
AcyI GRCGYC 2 cut(s) 5, 302
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 1 cut(s) 368
Alw21I GWGCWC 1 cut(s) 38
AlwI GGATC 1 cut(s) 30
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 1 cut(s) 102
Asp718I GGTACC 1 cut(s) 366
AspA2I CCTAGG 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 203, 355
AvrII CCTAGG 1 cut(s) 110
BanI GGYRCC 1 cut(s) 366
BbrPI CACGTG 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 58
BfaI CTAG 2 cut(s) 111, 329
BfmI CTRYAG 1 cut(s) 285
BisI GCNGC 1 cut(s) 72
BlnI CCTAGG 1 cut(s) 110
BlsI GCNGC 1 cut(s) 73
BmiI GGNNCC 2 cut(s) 147, 368
BmsI GCATC 2 cut(s) 47, 336
Bpu10I CCTNAGC 1 cut(s) 75
BsaAI YACGTR 1 cut(s) 137
BsaHI GRCGYC 2 cut(s) 5, 302
BsaJI CCNNGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 110
BseMI GCAATG 1 cut(s) 315
BseXI GCAGC 1 cut(s) 58
BshFI GGCC 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 38
BsnI GGCC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 1 cut(s) 22
BspACI CCGC 2 cut(s) 107, 149
BspANI GGCC 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 147, 368
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 315
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 1 cut(s) 22
BssNI GRCGYC 2 cut(s) 5, 302
BssT1I CCWWGG 1 cut(s) 110
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 2 cut(s) 5, 302
BstBAI YACGTR 1 cut(s) 137
BstDEI CTNAG 1 cut(s) 75
BstKTI GATC 1 cut(s) 25
BstMBI GATC 1 cut(s) 22
BstNSI RCATGY 1 cut(s) 132
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 367
CviAII CATG 1 cut(s) 129
CviJI RGCY 3 cut(s) 71, 98, 298
CviKI_1 RGCY 3 cut(s) 71, 98, 298
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 1 cut(s) 75
DpnI GATC 1 cut(s) 24
DpnII GATC 1 cut(s) 22
DraIII CACNNNGTG 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 296
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
EciI GGCGGA 1 cut(s) 96
Eco130I CCWWGG 1 cut(s) 110
Eco72I CACGTG 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 110
FaeI CATG 1 cut(s) 132
FaiI YATR 7 cut(s) 93, 130, 237, 263, 265, 385, 387
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FatI CATG 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 72
FspBI CTAG 2 cut(s) 111, 329
GluI GCNGC 1 cut(s) 72
HaeIII GGCC 1 cut(s) 298
Hin1I GRCGYC 2 cut(s) 5, 302
Hin1II CATG 1 cut(s) 132
HphI GGTGA 2 cut(s) 203, 355
Hpy188I TCNGA 2 cut(s) 83, 208
Hpy99I CGWCG 2 cut(s) 10, 304
HpyAV CCTTC 2 cut(s) 221, 403
HpyCH4IV ACGT 3 cut(s) 5, 136, 302
HpyCH4V TGCA 2 cut(s) 60, 287
HpyF3I CTNAG 1 cut(s) 75
HpySE526I ACGT 3 cut(s) 5, 136, 302
Hsp92I GRCGYC 2 cut(s) 5, 302
Hsp92II CATG 1 cut(s) 132
KpnI GGTACC 1 cut(s) 370
Kzo9I GATC 1 cut(s) 22
LpnPI CCDG 2 cut(s) 112, 155
Lsp1109I GCAGC 1 cut(s) 58
LweI GCATC 2 cut(s) 47, 336
MaeI CTAG 2 cut(s) 111, 329
MaeII ACGT 3 cut(s) 5, 136, 302
MalI GATC 1 cut(s) 24
MboI GATC 1 cut(s) 22
MboII GAAGA 2 cut(s) 155, 317
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 4 cut(s) 17, 102, 152, 258
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 1 cut(s) 360
MseI TTAA 4 cut(s) 12, 176, 228, 257
NdeII GATC 1 cut(s) 22
NlaIII CATG 1 cut(s) 132
NlaIV GGNNCC 2 cut(s) 147, 368
NspI RCATGY 1 cut(s) 132
PkrI GCNGC 1 cut(s) 73
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
Ppu21I YACGTR 1 cut(s) 137
PspCI CACGTG 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 147, 368
PstI CTGCAG 1 cut(s) 289
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 12, 176, 228, 257
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 1 cut(s) 22
SduI GDGCHC 1 cut(s) 38
SetI ASST 8 cut(s) 8, 81, 139, 182, 213, 234, 305, 372
SfaNI GCATC 2 cut(s) 47, 336
SfcI CTRYAG 1 cut(s) 285
Sse9I AATT 4 cut(s) 17, 102, 152, 258
SsiI CCGC 2 cut(s) 107, 149
SspMI CTAG 2 cut(s) 111, 329
StyI CCWWGG 1 cut(s) 110
TaiI ACGT 3 cut(s) 8, 139, 305
TaqI TCGA 1 cut(s) 220
TasI AATT 4 cut(s) 17, 102, 152, 258
Tru1I TTAA 4 cut(s) 12, 176, 228, 257
Tru9I TTAA 4 cut(s) 12, 176, 228, 257
TseI GCWGC 1 cut(s) 71
TspDTI ATGAA 2 cut(s) 364, 385
XapI RAATTY 1 cut(s) 102
XceI RCATGY 1 cut(s) 132
XmaJI CCTAGG 1 cut(s) 110
XspI CTAG 2 cut(s) 111, 329
ZraI GACGTC 2 cut(s) 6, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.