pycom05g22680

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0021264
Physical Location & Seq
Forward (+)
24917158 .. 24917813
656 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22680.2

Sequence Viewer

Length: 339 bp
ATGGCTCCTGAGCTTCTTAATGGTGGCAGCAGTAAGGTTTCTGAAAAGGTTGATGTATTCTCCTTTGGTATTGTCTTATGGGAGATTCTAACCGGTGAGGAGCCTTATGCCAATATGCATTACGGAGCAATCATAGGAGGCATTGTGAATAACACATTGAGGCCACATGTGCCGCCCTTTTGTGATTCTGAGTGGAAGTTGCTAATGGAGGAATGCTGGGCCGCGGATCCTGTTGCTCGCCCGTCGTTTACAGAAATAACCAAACGCTTGAGGGTGATGACTGCAGCTTGCCGGCCTACTAAACCACCGGTTCAGCCACAGAACCAGGTCCCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1 Pkinase_Tyr

Protein Analysis

113

Amino Acids

12.45

Weight (kDa)

6.09

Isoelectric Point (pI)

74.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021264)

Species Orthologous Gene IDs
pyrus_communis pycom05g22680 pycom13g22920
rosa_multiflora Rmu_co8298551.1_g000001
rosa_samantha Rh5CG161900 Rh5CG185200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 224
AciI CCGC 3 cut(s) 173, 222, 224
AclWI GGATC 2 cut(s) 221, 234
AflIII ACRYGT 1 cut(s) 166
AgeI ACCGGT 2 cut(s) 92, 307
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 2 cut(s) 13, 287
AluI AGCT 2 cut(s) 13, 287
AlwI GGATC 2 cut(s) 221, 234
AoxI GGCC 3 cut(s) 161, 219, 293
ApeKI GCWGC 2 cut(s) 27, 284
AsiGI ACCGGT 2 cut(s) 92, 307
AspS9I GGNCC 2 cut(s) 219, 328
AsuHPI GGTGA 2 cut(s) 107, 286
AvaII GGWCC 1 cut(s) 328
BamHI GGATCC 1 cut(s) 226
BbvI GCAGC 2 cut(s) 39, 296
BciT130I CCWGG 1 cut(s) 326
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 4 cut(s) 28, 173, 222, 285
BlsI GCNGC 4 cut(s) 29, 174, 223, 286
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 2 cut(s) 219, 328
BmiI GGNNCC 4 cut(s) 6, 102, 228, 330
BmrFI CCNGG 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 289
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 92, 307
BsaXI ACNNNNNCTCC 2 cut(s) 129, 159
Bse118I RCCGGY 3 cut(s) 92, 291, 307
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 39, 296
BseYI CCCAGC 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 224
BshFI GGCC 3 cut(s) 163, 221, 295
BshTI ACCGGT 2 cut(s) 92, 307
BsiSI CCGG 3 cut(s) 93, 292, 308
BslFI GGGAC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 3 cut(s) 163, 221, 295
Bsp143I GATC 1 cut(s) 226
BspACI CCGC 3 cut(s) 173, 222, 224
BspANI GGCC 3 cut(s) 163, 221, 295
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 1 cut(s) 224
BspLI GGNNCC 4 cut(s) 6, 102, 228, 330
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 2 cut(s) 221, 234
BsrFI RCCGGY 3 cut(s) 92, 291, 307
BssAI RCCGGY 3 cut(s) 92, 291, 307
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 1 cut(s) 226
Bst2UI CCWGG 1 cut(s) 326
BstC8I GCNNGC 3 cut(s) 238, 289, 293
BstDEI CTNAG 2 cut(s) 9, 189
BstDSI CCRYGG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 224
BstKTI GATC 1 cut(s) 229
BstMBI GATC 1 cut(s) 226
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 282
BstUI CGCG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 39, 296
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuRI GGCC 3 cut(s) 163, 221, 295
BtgI CCRYGG 1 cut(s) 222
Cac8I GCNNGC 3 cut(s) 238, 289, 293
Cfr10I RCCGGY 3 cut(s) 92, 291, 307
Cfr13I GGNCC 2 cut(s) 219, 328
Cfr42I CCGCGG 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 324
CspAI ACCGGT 2 cut(s) 92, 307
CviAII CATG 1 cut(s) 167
CviJI RGCY 8 cut(s) 5, 13, 103, 163, 221, 287, 295, 316
CviKI_1 RGCY 8 cut(s) 5, 13, 103, 163, 221, 287, 295, 316
DdeI CTNAG 2 cut(s) 9, 189
DpnI GATC 1 cut(s) 228
DpnII GATC 1 cut(s) 226
Eco47I GGWCC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 120
FaeI CATG 1 cut(s) 170
FaiI YATR 5 cut(s) 79, 108, 116, 134, 168
FaqI GGGAC 1 cut(s) 314
FatI CATG 1 cut(s) 166
Fnu4HI GCNGC 4 cut(s) 28, 173, 222, 285
Fsp4HI GCNGC 4 cut(s) 28, 173, 222, 285
GluI GCNGC 4 cut(s) 28, 173, 222, 285
GsaI CCCAGC 1 cut(s) 220
HaeIII GGCC 3 cut(s) 163, 221, 295
HapII CCGG 3 cut(s) 93, 292, 308
Hin1II CATG 1 cut(s) 170
HinfI GANTC 2 cut(s) 85, 185
HpaII CCGG 3 cut(s) 93, 292, 308
HphI GGTGA 2 cut(s) 107, 286
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 2 cut(s) 43, 190
Hpy188III TCNNGA 1 cut(s) 8
Hpy8I GTNNAC 1 cut(s) 249
Hpy99I CGWCG 1 cut(s) 247
HpyCH4V TGCA 2 cut(s) 118, 284
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 2 cut(s) 9, 189
Hsp92II CATG 1 cut(s) 170
KroI GCCGGC 1 cut(s) 291
KroNI GCCGGC 1 cut(s) 293
KspI CCGCGG 1 cut(s) 225
Kzo9I GATC 1 cut(s) 226
LmnI GCTCC 3 cut(s) 10, 100, 125
LpnPI CCDG 7 cut(s) 21, 106, 202, 243, 305, 311, 321
Lsp1109I GCAGC 2 cut(s) 39, 296
MabI ACCWGGT 1 cut(s) 324
MalI GATC 1 cut(s) 228
MboI GATC 1 cut(s) 226
MflI RGATCY 1 cut(s) 226
MnlI CCTC 5 cut(s) 91, 131, 153, 202, 264
Mph1103I ATGCAT 1 cut(s) 120
MroNI GCCGGC 1 cut(s) 291
MseI TTAA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 3 cut(s) 93, 292, 308
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 169
NaeI GCCGGC 1 cut(s) 293
NdeII GATC 1 cut(s) 226
NgoMIV GCCGGC 1 cut(s) 291
NlaIII CATG 1 cut(s) 170
NlaIV GGNNCC 4 cut(s) 6, 102, 228, 330
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 170
PciI ACATGT 1 cut(s) 166
PctI GAATGC 1 cut(s) 218
PdiI GCCGGC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 85, 185
PinAI ACCGGT 2 cut(s) 92, 307
PkrI GCNGC 4 cut(s) 29, 174, 223, 286
PpuMI RGGWCCY 1 cut(s) 328
PscI ACATGT 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 328
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 4 cut(s) 6, 102, 228, 330
PspPI GGNCC 2 cut(s) 219, 328
PspPPI RGGWCCY 1 cut(s) 328
PstI CTGCAG 1 cut(s) 286
PsuI RGATCY 1 cut(s) 226
SacII CCGCGG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 4 cut(s) 28, 173, 222, 285
Sau3AI GATC 1 cut(s) 226
Sau96I GGNCC 2 cut(s) 219, 328
ScrFI CCNGG 1 cut(s) 326
SetI ASST 5 cut(s) 15, 39, 51, 289, 330
SexAI ACCWGGT 1 cut(s) 324
SfcI CTRYAG 1 cut(s) 282
Sfr303I CCGCGG 1 cut(s) 225
SgrBI CCGCGG 1 cut(s) 225
SinI GGWCC 1 cut(s) 328
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
SsiI CCGC 3 cut(s) 173, 222, 224
StyD4I CCNGG 1 cut(s) 324
TauI GCSGC 2 cut(s) 175, 224
TfiI GAWTC 2 cut(s) 85, 185
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TseI GCWGC 2 cut(s) 27, 284
TspGWI ACGGA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 328
XceI RCATGY 1 cut(s) 170
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.