pycom05g25270

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016285
Physical Location & Seq
Forward (+)
26703911 .. 26704755
845 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25270.1

Sequence Viewer

Length: 651 bp
ATGGCCACTGATATTTCCTTCTTTGGGTTTGTATTATCTTTATATTTGTCAATCGCCATTGCATCGGATCATAGTCCACTTCAAGATTTCTGTGTTGGGGACCCGAAAAGTCAAGTGGTGGTCAATGGTCTAGCTTGCAAGGACCCTAAGACAGTTGAAGACAATGATTTTTCATTTGGTGGACTTCATTTAGAAGGCAACACATCTAACCCAATTGGGTCAAGTGTGACACCAGTAACCGTGTTTCAACTACCAGGTCTCAACACTCTTGGCATTTCACTTGTCCGAATCGACTATGCACCATCAGGTGTCAACCCTCCACACATTCATCCTCGCGCCTCCGAAGTTTTAACACTCTTGGAAGGCAGCCTTGAAGTCGGGTTTGTGACATCCAGCCCCGAGAACCGTCACATCACTAAGGTGTTGCAGAAGGGTGATGTGTTTGTTTTTCCAATTGGACTTGTGCACTATCAGAGAAATGTAGGTTCTGGTAATGCTGTGGCTATTGCAGCTCTCAGCAGCCAAAATCCCGGTGTGGTGATAATTGCCAGTTCTGTTTTTGGGTCCACGCCTAGTATTGTCAATGACATTCTTGTGAAGGCATTCCATGTTGATAAAGGTATTGTCAACTACCTCCATTCGATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

217

Amino Acids

22.87

Weight (kDa)

5.66

Isoelectric Point (pI)

34.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 65 - 206 3.2e-46 Cupin
Cupin_2 PF07883 97 - 169 4.1e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016285)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10630 FvH4_3g10630
malus_domestica MD05G1269800.v1.1
prunus_persica Prupe.4G094700_v2.0.a1
pyrus_communis pycom05g25270
rosa_chinensis RchiOBHm_Chr5g0017341
rosa_laevigata RLG00000032275
rosa_multiflora Rmu_sc0005223.1_g000005 Rmu_sc0005319.1_g000008
rosa_roxburghii Rroxscaffold_1G00060020
rosa_rugosa Rorug05G0034100
rosa_samantha Rh5AG129200 Rh5BG127000 Rh5CG139800
rosa_wichuraiana Rw5G011160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 336
AclWI GGATC 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 1 cut(s) 24
AgsI TTSAA 4 cut(s) 83, 158, 248, 374
AjnI CCWGG 1 cut(s) 253
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 2 cut(s) 134, 512
AluI AGCT 2 cut(s) 134, 512
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 1 cut(s) 263
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 1 cut(s) 75
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 3 cut(s) 366, 509, 519
Asp700I GAANNNNTTC 1 cut(s) 602
AspLEI GCGC 1 cut(s) 338
AspS9I GGNCC 3 cut(s) 100, 142, 564
AsuC2I CCSGG 1 cut(s) 531
AsuHPI GGTGA 2 cut(s) 446, 550
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 3 cut(s) 100, 142, 564
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 3 cut(s) 378, 521, 531
BccI CCATC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 531
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 2 cut(s) 131, 573
BisI GCNGC 3 cut(s) 367, 510, 520
BlsI GCNGC 3 cut(s) 368, 511, 521
Bme1390I CCNGG 2 cut(s) 255, 531
Bme18I GGWCC 3 cut(s) 100, 142, 564
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 100, 142, 564
BmiI GGNNCC 4 cut(s) 101, 102, 144, 565
BmrFI CCNGG 2 cut(s) 255, 531
BmsI GCATC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 531
BsaI GGTCTC 1 cut(s) 263
Bsc4I CCNNNNNNNGG 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 233, 549
Bse3DI GCAATG 1 cut(s) 57
BseBI CCWGG 1 cut(s) 255
BseGI GGATG 2 cut(s) 328, 389
BseLI CCNNNNNNNGG 1 cut(s) 24
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 1 cut(s) 529
BseNI ACTGG 2 cut(s) 233, 549
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 3 cut(s) 378, 521, 531
Bsh1236I CGCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 24
BsmAI GTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 1 cut(s) 67
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 528
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 4 cut(s) 101, 102, 144, 565
BspPI GGATC 1 cut(s) 75
BspTNI GGTCTC 1 cut(s) 263
BsrDI GCAATG 1 cut(s) 57
BsrI ACTGG 2 cut(s) 233, 549
BssMI GATC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 3 cut(s) 154, 241, 407
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 3 cut(s) 147, 417, 515
BstF5I GGATG 2 cut(s) 328, 389
BstFNI CGCG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 338
BstKTI GATC 1 cut(s) 70
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 1 cut(s) 67
BstMWI GCNNNNNNNGC 1 cut(s) 509
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 2 cut(s) 253, 529
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 378, 521, 531
BstV2I GAAGAC 1 cut(s) 165
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 328, 389
BtsIMutI CAGTG 1 cut(s) 6
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 100, 142, 564
CsiI ACCWGGT 1 cut(s) 253
CviAII CATG 1 cut(s) 608
CviJI RGCY 7 cut(s) 5, 134, 369, 396, 503, 512, 522
CviKI_1 RGCY 7 cut(s) 5, 134, 369, 396, 503, 512, 522
DdeI CTNAG 3 cut(s) 147, 417, 515
DpnI GATC 1 cut(s) 69
DpnII GATC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 3 cut(s) 100, 142, 564
Eco88I CYCGRG 1 cut(s) 398
EcoO109I RGGNCCY 2 cut(s) 100, 142
EcoRII CCWGG 1 cut(s) 253
FaeI CATG 1 cut(s) 611
FaiI YATR 4 cut(s) 43, 72, 297, 609
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FaqI GGGAC 1 cut(s) 113
FatI CATG 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 367, 510, 520
FokI GGATG 2 cut(s) 315, 376
Fsp4HI GCNGC 3 cut(s) 367, 510, 520
FspBI CTAG 2 cut(s) 131, 573
GlaI GCGC 1 cut(s) 337
GluI GCNGC 3 cut(s) 367, 510, 520
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 531
HhaI GCGC 1 cut(s) 338
Hin1II CATG 1 cut(s) 611
Hin6I GCGC 1 cut(s) 336
HinP1I GCGC 1 cut(s) 336
HincII GTYRAC 2 cut(s) 313, 628
HindII GTYRAC 2 cut(s) 313, 628
HinfI GANTC 1 cut(s) 288
HpaII CCGG 1 cut(s) 531
HphI GGTGA 2 cut(s) 446, 550
Hpy166II GTNNAC 6 cut(s) 77, 182, 313, 466, 567, 628
Hpy188I TCNGA 4 cut(s) 67, 287, 343, 474
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 6 cut(s) 77, 182, 313, 466, 567, 628
HpyAV CCTTC 5 cut(s) 28, 188, 356, 424, 592
HpyCH4III ACNGT 3 cut(s) 154, 241, 407
HpyCH4V TGCA 6 cut(s) 62, 138, 299, 427, 466, 509
HpyF10VI GCNNNNNNNGC 1 cut(s) 509
HpyF3I CTNAG 3 cut(s) 147, 417, 515
Hsp92II CATG 1 cut(s) 611
HspAI GCGC 1 cut(s) 336
KflI GGGWCCC 1 cut(s) 100
Kzo9I GATC 1 cut(s) 67
LpnPI CCDG 8 cut(s) 240, 246, 267, 291, 406, 474, 544, 562
Lsp1109I GCAGC 3 cut(s) 378, 521, 531
LweI GCATC 1 cut(s) 71
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 2 cut(s) 131, 573
MaeIII GTNAC 4 cut(s) 226, 235, 385, 407
MalI GATC 1 cut(s) 69
MboI GATC 1 cut(s) 67
MboII GAAGA 1 cut(s) 170
MfeI CAATTG 2 cut(s) 213, 453
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 213, 453, 543
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 327, 342, 349, 644
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 602
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 350
MslI CAYNNNNRTG 2 cut(s) 419, 593
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 2 cut(s) 255, 531
MunI CAATTG 2 cut(s) 213, 453
Mva1269I GAATGC 1 cut(s) 602
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 1 cut(s) 509
NciI CCSGG 1 cut(s) 531
NdeII GATC 1 cut(s) 67
NlaIII CATG 1 cut(s) 611
NlaIV GGNNCC 4 cut(s) 101, 102, 144, 565
NmuCI GTSAC 3 cut(s) 226, 385, 407
OliI CACNNNNGTG 1 cut(s) 419
PctI GAATGC 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 288
PkrI GCNGC 3 cut(s) 368, 511, 521
PpuMI RGGWCCY 2 cut(s) 100, 142
Psp5II RGGWCCY 2 cut(s) 100, 142
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 4 cut(s) 101, 102, 144, 565
PspPI GGNCC 3 cut(s) 100, 142, 564
PspPPI RGGWCCY 2 cut(s) 100, 142
RseI CAYNNNNRTG 2 cut(s) 419, 593
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 3 cut(s) 367, 510, 520
Sau3AI GATC 1 cut(s) 67
Sau96I GGNCC 3 cut(s) 100, 142, 564
ScrFI CCNGG 2 cut(s) 255, 531
SduI GDGCHC 1 cut(s) 468
SetI ASST 8 cut(s) 136, 259, 310, 423, 487, 514, 622, 636
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 1 cut(s) 71
SinI GGWCC 3 cut(s) 100, 142, 564
SmiMI CAYNNNNRTG 2 cut(s) 419, 593
Sse9I AATT 3 cut(s) 213, 453, 543
SspMI CTAG 2 cut(s) 131, 573
StyD4I CCNGG 2 cut(s) 253, 529
TaaI ACNGT 3 cut(s) 154, 241, 407
TaqI TCGA 2 cut(s) 291, 641
TasI AATT 3 cut(s) 213, 453, 543
TfiI GAWTC 1 cut(s) 288
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 1 cut(s) 13
TseFI GTSAC 3 cut(s) 226, 385, 407
TseI GCWGC 3 cut(s) 366, 509, 519
Tsp45I GTSAC 3 cut(s) 226, 385, 407
TspDTI ATGAA 3 cut(s) 162, 176, 317
TspRI CASTG 1 cut(s) 13
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 3 cut(s) 100, 142, 564
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 2 cut(s) 131, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.