RLG00000032275

Cupin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016285
Physical Location & Seq
Reverse (-)
12981834 .. 12983055
1222 bp
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UTR
Exon/CDS
Intron
RLM00000032275

Sequence Viewer

Length: 651 bp
ATGGGCACTTATAATATCATCTTTGGGTTCCTGTTATCTTTCTCTTTGTCAGTTGCCACAGCATCTGATCATAGTCCACTTCAAGATTTCTGTGTGGCAGACCCAGAAAGTCGAGTGCTGCTGAATGGCCTAGCTTGCAAGGACCCAAAGACAGTTGAAGCCAACGATTTTTCATTTAGTGGGCTTCACTTAGCAGCCAATACATCAAACCCAATCGGGTCCAGTGTGACACCGGTGACTGTATCCCAACTTCCAGGACTTAACACTCTTGGCATTTCACTAGTCCGCATTGACTATGCACCATCAGGCATCAACCCTCCCCACACTCATCCTCGTGCCTCTGAAATATTGACCATCCTCGAAGGCAGCCTTGAAGTCGGGTTCGTCACTTCCAGCCCCGAAAATCGTCACATCACCAAGGTGTTGCAGAAGGGTGATGTGTTTGTATTCCCAATCGGACTCGTCCACTACCAGAGAAATATAGGAACTGGCAATGCTGTTGCTATTGCTGCGTTGAGCAGCCAAAATCCTGGTGTAGTAATAATTGCAAGTGCTGTCTTTGGGTCCAGGCCTAGTATTTGTAGTGAGATTCTCGTGAAGGCTTTGCAAGTTGATCAAGGGGTTGTCAATTACCTCCAGTCCATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

217

Amino Acids

22.8

Weight (kDa)

5.8

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 62 - 207 2.4e-48 Cupin
Cupin_2 PF07883 97 - 169 1.1e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016285)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10630 FvH4_3g10630
malus_domestica MD05G1269800.v1.1
prunus_persica Prupe.4G094700_v2.0.a1
pyrus_communis pycom05g25270
rosa_chinensis RchiOBHm_Chr5g0017341
rosa_laevigata RLG00000032275
rosa_multiflora Rmu_sc0005223.1_g000005 Rmu_sc0005319.1_g000008
rosa_roxburghii Rroxscaffold_1G00060020
rosa_rugosa Rorug05G0034100
rosa_samantha Rh5AG129200 Rh5BG127000 Rh5CG139800
rosa_wichuraiana Rw5G011160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
AciI CCGC 1 cut(s) 286
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 3 cut(s) 83, 158, 374
AhlI ACTAGT 1 cut(s) 280
AjnI CCWGG 3 cut(s) 253, 529, 566
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 2 cut(s) 127, 569
ApeKI GCWGC 5 cut(s) 118, 194, 366, 509, 519
AsiGI ACCGGT 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 142, 219, 564
AsuHPI GGTGA 3 cut(s) 247, 406, 446
AvaII GGWCC 3 cut(s) 142, 219, 564
BaeGI GKGCMC 1 cut(s) 8
BarI GAAGNNNNNNTAC 2 cut(s) 234, 266
BauI CACGAG 2 cut(s) 333, 593
BbvI GCAGC 5 cut(s) 105, 206, 378, 496, 531
BccI CCATC 2 cut(s) 310, 362
BciT130I CCWGG 3 cut(s) 255, 531, 568
BciVI GTATCC 1 cut(s) 253
BclI TGATCA 2 cut(s) 67, 613
BcuI ACTAGT 1 cut(s) 280
BfaI CTAG 3 cut(s) 131, 281, 573
BfuI GTATCC 1 cut(s) 253
BisI GCNGC 5 cut(s) 119, 195, 367, 510, 520
BlsI GCNGC 5 cut(s) 120, 196, 368, 511, 521
Bme1390I CCNGG 3 cut(s) 255, 531, 568
Bme18I GGWCC 3 cut(s) 142, 219, 564
BmgT120I GGNCC 3 cut(s) 142, 219, 564
BmiI GGNNCC 4 cut(s) 29, 144, 220, 565
BmrFI CCNGG 3 cut(s) 255, 531, 568
BmsI GCATC 2 cut(s) 71, 318
BpmI CTGGAG 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 417
BsaWI WCCGGW 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 3 cut(s) 222, 493, 637
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 3 cut(s) 255, 531, 568
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 2 cut(s) 328, 354
BseMI GCAATG 1 cut(s) 499
BseNI ACTGG 3 cut(s) 222, 493, 637
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 5 cut(s) 105, 206, 378, 496, 531
BshFI GGCC 2 cut(s) 129, 571
BshTI ACCGGT 1 cut(s) 232
BsiSI CCGG 1 cut(s) 233
BsnI GGCC 2 cut(s) 129, 571
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 67, 613
BspACI CCGC 1 cut(s) 286
BspANI GGCC 2 cut(s) 129, 571
BspLI GGNNCC 4 cut(s) 29, 144, 220, 565
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 3 cut(s) 222, 493, 637
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 2 cut(s) 67, 613
BssSI CACGAG 2 cut(s) 333, 593
BssT1I CCWWGG 1 cut(s) 417
Bst2BI CACGAG 2 cut(s) 333, 593
Bst2UI CCWGG 3 cut(s) 255, 531, 568
Bst4CI ACNGT 2 cut(s) 154, 241
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 1 cut(s) 190
BstF5I GGATG 2 cut(s) 328, 354
BstKTI GATC 2 cut(s) 70, 616
BstMBI GATC 2 cut(s) 67, 613
BstMWI GCNNNNNNNGC 2 cut(s) 135, 509
BstNI CCWGG 3 cut(s) 255, 531, 568
BstSCI CCNGG 3 cut(s) 253, 529, 566
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 5 cut(s) 105, 206, 378, 496, 531
BstXI CCANNNNNNTGG 1 cut(s) 530
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 2 cut(s) 129, 571
BtsCI GGATG 2 cut(s) 328, 354
BtsIMutI CAGTG 1 cut(s) 229
Cac8I GCNNGC 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 65
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 3 cut(s) 142, 219, 564
CspAI ACCGGT 1 cut(s) 232
DdeI CTNAG 1 cut(s) 190
DpnI GATC 2 cut(s) 69, 615
DpnII GATC 2 cut(s) 67, 613
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 571
Eco47I GGWCC 3 cut(s) 142, 219, 564
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 3 cut(s) 253, 529, 566
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
FaiI YATR 5 cut(s) 12, 72, 297, 482, 644
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FbaI TGATCA 2 cut(s) 67, 613
Fnu4HI GCNGC 5 cut(s) 119, 195, 367, 510, 520
FokI GGATG 2 cut(s) 315, 341
Fsp4HI GCNGC 5 cut(s) 119, 195, 367, 510, 520
FspBI CTAG 3 cut(s) 131, 281, 573
GluI GCNGC 5 cut(s) 119, 195, 367, 510, 520
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 2 cut(s) 129, 571
HapII CCGG 1 cut(s) 233
HinfI GANTC 2 cut(s) 459, 589
HpaII CCGG 1 cut(s) 233
HphI GGTGA 3 cut(s) 247, 406, 446
Hpy166II GTNNAC 2 cut(s) 77, 466
Hpy188I TCNGA 3 cut(s) 67, 343, 458
Hpy188III TCNNGA 2 cut(s) 83, 595
Hpy8I GTNNAC 2 cut(s) 77, 466
HpyAV CCTTC 3 cut(s) 356, 424, 592
HpyCH4III ACNGT 2 cut(s) 154, 241
HpyCH4V TGCA 5 cut(s) 138, 299, 427, 548, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 509
HpyF3I CTNAG 1 cut(s) 190
Ksp22I TGATCA 2 cut(s) 67, 613
Kzo9I GATC 2 cut(s) 67, 613
Lsp1109I GCAGC 5 cut(s) 105, 206, 378, 496, 531
LweI GCATC 2 cut(s) 71, 318
MaeI CTAG 3 cut(s) 131, 281, 573
MaeIII GTNAC 4 cut(s) 226, 235, 385, 407
MalI GATC 2 cut(s) 69, 615
MboI GATC 2 cut(s) 67, 613
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 543, 628
MlyI GAGTC 1 cut(s) 453
MnlI CCTC 5 cut(s) 327, 342, 349, 368, 644
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 2 cut(s) 333, 419
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 255, 531, 568
MvaI CCWGG 3 cut(s) 255, 531, 568
MwoI GCNNNNNNNGC 2 cut(s) 135, 509
NdeII GATC 2 cut(s) 67, 613
NlaIV GGNNCC 4 cut(s) 29, 144, 220, 565
NmuCI GTSAC 4 cut(s) 226, 235, 385, 407
OliI CACNNNNGTG 1 cut(s) 419
PceI AGGCCT 1 cut(s) 571
PfeI GAWTC 1 cut(s) 589
PfoI TCCNGGA 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 5 cut(s) 120, 196, 368, 511, 521
PleI GAGTC 1 cut(s) 453
PpsI GAGTC 1 cut(s) 453
PpuMI RGGWCCY 1 cut(s) 142
PsiI TTATAA 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 3 cut(s) 253, 529, 566
PspGI CCWGG 3 cut(s) 253, 529, 566
PspN4I GGNNCC 4 cut(s) 29, 144, 220, 565
PspPI GGNCC 3 cut(s) 142, 219, 564
PspPPI RGGWCCY 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 65
RseI CAYNNNNRTG 2 cut(s) 333, 419
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 5 cut(s) 119, 195, 367, 510, 520
Sau3AI GATC 2 cut(s) 67, 613
Sau96I GGNCC 3 cut(s) 142, 219, 564
SchI GAGTC 1 cut(s) 453
ScrFI CCNGG 3 cut(s) 255, 531, 568
SduI GDGCHC 1 cut(s) 8
SetI ASST 3 cut(s) 136, 423, 636
SfaNI GCATC 2 cut(s) 71, 318
SgrAI CRCCGGYG 1 cut(s) 232
SinI GGWCC 3 cut(s) 142, 219, 564
SmiMI CAYNNNNRTG 2 cut(s) 333, 419
SpeI ACTAGT 1 cut(s) 280
Sse9I AATT 2 cut(s) 543, 628
SseBI AGGCCT 1 cut(s) 571
SsiI CCGC 1 cut(s) 286
SspI AATATT 1 cut(s) 348
SspMI CTAG 3 cut(s) 131, 281, 573
StuI AGGCCT 1 cut(s) 571
StyD4I CCNGG 3 cut(s) 253, 529, 566
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 2 cut(s) 154, 241
TaqI TCGA 2 cut(s) 112, 360
TasI AATT 2 cut(s) 543, 628
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TscAI CASTG 1 cut(s) 229
TseFI GTSAC 4 cut(s) 226, 235, 385, 407
TseI GCWGC 5 cut(s) 118, 194, 366, 509, 519
Tsp45I GTSAC 4 cut(s) 226, 235, 385, 407
TspDTI ATGAA 1 cut(s) 162
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 3 cut(s) 142, 219, 564
XspI CTAG 3 cut(s) 131, 281, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.