pycom05g25280

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019818
Physical Location & Seq
Forward (+)
26708321 .. 26708824
504 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25280.1

Sequence Viewer

Length: 504 bp
ATGGCCATAAACGGGAGGACCATGCTTATTCAACTCTCTTTGGCCGTAATATTAGCTGCTACAATTACAAAATTAGCAGCAGCTGATGGACCCCTGACTCAAGCCCTGCCTGGCTGCAAAGACAAGTGTGGTGATCTCACAATTCCATATCCATTTGGCATAGGTGACGGTTGTTACCTCCGACCGGAATTCAACATCACTTGTGACCACTCGGCCACACCCCCAGCGGCAAACTTAACTGGTCAGACATTCCGTATTACCAAATTTTCCCTTGATGAGGGTGAGTTGCAAGTAATGATGTTCATATCCCGAGATTGCTATGCCACGGTTGACTCCCACGCTCGCAGAACCTACCGGAACAACCCAGCACTCTGGTTGCCTCCTCCTTATACCATATCTGATACCAAGAACAGATTCATCGCAGTTGGTTGTGACACTTACGCCCTTTTCCAAGGTTATAGAGGCGAAGAAAGGTTCATCACTGGGTGCATGTTGTTAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

168

Amino Acids

18.32

Weight (kDa)

6.7

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 98 1.2e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019818)

Species Orthologous Gene IDs
pyrus_communis pycom05g25280
rosa_chinensis RchiOBHm_Chr5g0016941
rosa_laevigata RLG00000032284
rosa_multiflora Rmu_sc0003630.1_g000063 Rmu_sc0003801.1_g000024 Rmu_sc0007023.1_g000008
rosa_rugosa Rorug05G0587100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 227
AcoI YGGCCR 3 cut(s) 3, 42, 213
AcsI RAATTY 2 cut(s) 188, 263
AdeI CACNNNGTG 1 cut(s) 486
AfiI CCNNNNNNNGG 3 cut(s) 12, 184, 277
AgsI TTSAA 2 cut(s) 32, 193
AjnI CCWGG 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 2 cut(s) 56, 83
AluI AGCT 2 cut(s) 56, 83
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 309
AoxI GGCC 3 cut(s) 3, 42, 213
ApeKI GCWGC 4 cut(s) 56, 77, 80, 114
ApoI RAATTY 2 cut(s) 188, 263
Asp700I GAANNNNTTC 1 cut(s) 413
AspS9I GGNCC 2 cut(s) 18, 89
AsuHPI GGTGA 3 cut(s) 143, 176, 293
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 2 cut(s) 18, 89
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 4 cut(s) 43, 89, 92, 101
BccI CCATC 1 cut(s) 80
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 111
BisI GCNGC 5 cut(s) 57, 78, 81, 115, 228
BlsI GCNGC 5 cut(s) 58, 79, 82, 116, 229
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 2 cut(s) 18, 89
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 2 cut(s) 18, 89
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 111
BmrI ACTGGG 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 324, 451
BsaWI WCCGGW 2 cut(s) 184, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 12, 184, 277
Bse1I ACTGG 2 cut(s) 244, 487
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 324, 451
BseLI CCNNNNNNNGG 3 cut(s) 12, 184, 277
BseNI ACTGG 2 cut(s) 244, 487
BseRI GAGGAG 1 cut(s) 372
BseXI GCAGC 4 cut(s) 43, 89, 92, 101
BseYI CCCAGC 2 cut(s) 223, 364
Bsh1285I CGRYCG 1 cut(s) 185
BshFI GGCC 3 cut(s) 5, 44, 215
BsiEI CGRYCG 1 cut(s) 185
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 185, 355
BslI CCNNNNNNNGG 3 cut(s) 12, 184, 277
BsnI GGCC 3 cut(s) 5, 44, 215
BsoBI CYCGRG 1 cut(s) 309
Bsp143I GATC 1 cut(s) 133
BspACI CCGC 1 cut(s) 227
BspANI GGCC 3 cut(s) 5, 44, 215
BspLI GGNNCC 1 cut(s) 91
BsrI ACTGG 2 cut(s) 244, 487
BssECI CCNNGG 2 cut(s) 324, 451
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 170, 328
BstC8I GCNNGC 1 cut(s) 343
BstDSI CCRYGG 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 275
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMCI CGRYCG 1 cut(s) 185
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 493
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 4 cut(s) 43, 89, 92, 101
BstXI CCANNNNNNTGG 1 cut(s) 372
BsuRI GGCC 3 cut(s) 5, 44, 215
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 1 cut(s) 403
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 18, 89
CviAII CATG 2 cut(s) 22, 490
CviJI RGCY 7 cut(s) 5, 44, 56, 83, 104, 114, 215
CviKI_1 RGCY 7 cut(s) 5, 44, 56, 83, 104, 114, 215
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
DraIII CACNNNGTG 1 cut(s) 486
EaeI YGGCCR 3 cut(s) 3, 42, 213
Eco130I CCWWGG 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 18, 89
Eco88I CYCGRG 1 cut(s) 309
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 2 cut(s) 25, 493
FatI CATG 2 cut(s) 21, 489
Fnu4HI GCNGC 5 cut(s) 57, 78, 81, 115, 228
Fsp4HI GCNGC 5 cut(s) 57, 78, 81, 115, 228
GluI GCNGC 5 cut(s) 57, 78, 81, 115, 228
GsaI CCCAGC 2 cut(s) 227, 368
HaeIII GGCC 3 cut(s) 5, 44, 215
HapII CCGG 2 cut(s) 185, 355
Hin1II CATG 2 cut(s) 25, 493
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 3 cut(s) 97, 332, 414
HpaII CCGG 2 cut(s) 185, 355
HphI GGTGA 3 cut(s) 143, 176, 293
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 182, 246, 400
Hpy188III TCNNGA 1 cut(s) 309
Hpy8I GTNNAC 1 cut(s) 331
HpyCH4III ACNGT 2 cut(s) 170, 328
HpyCH4V TGCA 3 cut(s) 117, 289, 489
Hsp92II CATG 2 cut(s) 25, 493
Kzo9I GATC 1 cut(s) 133
Lsp1109I GCAGC 4 cut(s) 43, 89, 92, 101
MaeIII GTNAC 4 cut(s) 164, 173, 203, 431
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 1 cut(s) 479
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 63, 71, 141, 188, 263
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 91, 326
MmeI TCCRAC 1 cut(s) 205
MnlI CCTC 6 cut(s) 9, 188, 271, 390, 393, 455
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 236
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 83, 227
MspI CCGG 2 cut(s) 185, 355
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
NdeII GATC 1 cut(s) 133
NlaIII CATG 2 cut(s) 25, 493
NlaIV GGNNCC 1 cut(s) 91
NmeAIII GCCGAG 1 cut(s) 191
NmuCI GTSAC 3 cut(s) 164, 203, 431
NspI RCATGY 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 5 cut(s) 58, 79, 82, 116, 229
PleI GAGTC 2 cut(s) 91, 326
PpsI GAGTC 2 cut(s) 91, 326
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 2 cut(s) 223, 364
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 18, 89
PstNI CAGNNNCTG 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 83
SaqAI TTAA 1 cut(s) 236
SatI GCNGC 5 cut(s) 57, 78, 81, 115, 228
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 2 cut(s) 18, 89
SchI GAGTC 2 cut(s) 91, 326
ScrFI CCNGG 1 cut(s) 111
SetI ASST 7 cut(s) 58, 85, 166, 180, 353, 457, 476
SinI GGWCC 2 cut(s) 18, 89
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 5 cut(s) 63, 71, 141, 188, 263
SsiI CCGC 1 cut(s) 227
SspI AATATT 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 170, 328
TasI AATT 5 cut(s) 63, 71, 141, 188, 263
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 1 cut(s) 236
Tru9I TTAA 1 cut(s) 236
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 3 cut(s) 164, 203, 431
TseI GCWGC 4 cut(s) 56, 77, 80, 114
Tsp45I GTSAC 3 cut(s) 164, 203, 431
TspDTI ATGAA 3 cut(s) 292, 406, 466
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 18, 89
XagI CCTNNNNNAGG 1 cut(s) 275
XapI RAATTY 2 cut(s) 188, 263
XceI RCATGY 1 cut(s) 493
XmnI GAANNNNTTC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.