RchiOBHm_Chr5g0016941

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019818
Physical Location & Seq
Reverse (-)
11667024 .. 11667731
708 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29728

Sequence Viewer

Length: 708 bp
ATGGCGTCCTTACATAGGATGCTGCTTATGCAACTCTGTCTGATGGGACTGGTTGCAACTCAAACGCTGCCACCTCAAGCGAAGCTTTACTGTCTCTACTGGTGCGGTAATGTCCAGATTCCATATCCATTTGGTATAGGTGACGGTTGCTACCTACCAACAAATACTCGATTTAACCTCAGTTGCAATTTATCCACCACTCCTCCATCAACACGCTGGACAGGTATTACACTCACAATTACCGACATTTCGCTTGCGGAGAGTGAGCTGCAAATTATCACTTTCATAGCTAAAGAATGTTATAACGAGGAAGGCGAGATGACGTACAGCAAAAGCCCTTCTCTCCGCGTGTCTCCAGCTCCTTACACTATCTCTGACACCAAAAACAAATTCTTCACCGTCTGTTGCGACACATACGCACTTTTCCAAGGCTACCGGGACGAAAAGAGGTTCCAAACAGGGTGCATGTCGTTATGTGACGACCGTGAGAACATTTCAGACTCTTGCTCAGGCGTCGGGTGCTGTGAAACTGCTATCCCTAGCGGACTCAAAAACCGTACCATCAGGTTGAGTAGCTACTACAACAATTCCTATATAATGGGATTCAACCCCTGTAGTTACGCCTTCATTGTGCAAGACATGGCCAGTTCAGTTTCTCCACGAATACAAGTTTTGAGCATTCGAACAACACTGCAAAGCTTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

235

Amino Acids

26.23

Weight (kDa)

6.54

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 92 1e-09 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019818)

Species Orthologous Gene IDs
pyrus_communis pycom05g25280
rosa_chinensis RchiOBHm_Chr5g0016941
rosa_laevigata RLG00000032284
rosa_multiflora Rmu_sc0003630.1_g000063 Rmu_sc0003801.1_g000024 Rmu_sc0007023.1_g000008
rosa_rugosa Rorug05G0587100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AccII CGCG 1 cut(s) 348
AciI CCGC 4 cut(s) 105, 257, 346, 543
AcoI YGGCCR 1 cut(s) 642
AcsI RAATTY 1 cut(s) 389
AcyI GRCGYC 2 cut(s) 5, 513
AfaI GTAC 2 cut(s) 326, 559
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 6 cut(s) 85, 268, 290, 359, 576, 699
AluI AGCT 6 cut(s) 85, 268, 290, 359, 576, 699
Alw26I GTCTC 2 cut(s) 98, 357
AoxI GGCC 1 cut(s) 642
ApeKI GCWGC 3 cut(s) 22, 67, 268
ApoI RAATTY 1 cut(s) 389
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 152, 388
AsuII TTCGAA 1 cut(s) 682
BalI TGGCCA 1 cut(s) 644
BbvI GCAGC 3 cut(s) 9, 54, 255
BccI CCATC 3 cut(s) 37, 214, 569
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 98, 357
BfaI CTAG 1 cut(s) 540
BfmI CTRYAG 1 cut(s) 613
BisI GCNGC 3 cut(s) 23, 68, 269
BlsI GCNGC 3 cut(s) 24, 69, 270
Bme1390I CCNGG 1 cut(s) 437
BmiI GGNNCC 1 cut(s) 452
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 1 cut(s) 9
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 1 cut(s) 508
Bpu14I TTCGAA 1 cut(s) 682
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 437
BsaHI GRCGYC 2 cut(s) 5, 513
BsaJI CCNNGG 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 3 cut(s) 54, 104, 645
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMII CTCAG 2 cut(s) 193, 522
BseNI ACTGG 3 cut(s) 54, 104, 645
BseRI GAGGAG 1 cut(s) 192
BseXI GCAGC 3 cut(s) 9, 54, 255
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 484
BshFI GGCC 1 cut(s) 644
BsiEI CGRYCG 1 cut(s) 484
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 2 cut(s) 60, 452
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 2 cut(s) 98, 357
BsmFI GGGAC 2 cut(s) 60, 452
BsmI GAATGC 1 cut(s) 678
BsnI GGCC 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 682
BspACI CCGC 4 cut(s) 105, 257, 346, 543
BspANI GGCC 1 cut(s) 644
BspCNI CTCAG 2 cut(s) 192, 521
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 1 cut(s) 452
BspT104I TTCGAA 1 cut(s) 682
BsrI ACTGG 3 cut(s) 54, 104, 645
BssECI CCNNGG 1 cut(s) 427
BssNI GRCGYC 2 cut(s) 5, 513
BssT1I CCWWGG 1 cut(s) 427
Bst4CI ACNGT 5 cut(s) 92, 146, 400, 485, 557
BstACI GRCGYC 2 cut(s) 5, 513
BstBI TTCGAA 1 cut(s) 682
BstC8I GCNNGC 1 cut(s) 255
BstDEI CTNAG 2 cut(s) 179, 508
BstENI CCTNNNNNAGG 1 cut(s) 13
BstF5I GGATG 1 cut(s) 24
BstFNI CGCG 1 cut(s) 348
BstMAI GTCTC 2 cut(s) 98, 357
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 2 cut(s) 28, 519
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 435
BstSFI CTRYAG 1 cut(s) 613
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 9, 54, 255
BsuRI GGCC 1 cut(s) 644
BtsCI GGATG 1 cut(s) 24
BtsI GCAGTG 1 cut(s) 689
BtsIMutI CAGTG 1 cut(s) 689
Cac8I GCNNGC 1 cut(s) 255
CseI GACGC 1 cut(s) 502
Csp6I GTAC 2 cut(s) 325, 558
CviAII CATG 2 cut(s) 466, 640
CviJI RGCY 9 cut(s) 85, 268, 290, 336, 359, 432, 576, 644, 699
CviKI_1 RGCY 9 cut(s) 85, 268, 290, 336, 359, 432, 576, 644, 699
CviQI GTAC 2 cut(s) 325, 558
DdeI CTNAG 2 cut(s) 179, 508
EaeI YGGCCR 1 cut(s) 642
Eco130I CCWWGG 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 13
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 2 cut(s) 469, 643
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 2 cut(s) 60, 452
FatI CATG 2 cut(s) 465, 639
Fnu4HI GCNGC 3 cut(s) 23, 68, 269
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 23, 68, 269
FspBI CTAG 1 cut(s) 540
GluI GCNGC 3 cut(s) 23, 68, 269
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 1 cut(s) 644
HapII CCGG 1 cut(s) 436
HgaI GACGC 1 cut(s) 502
Hin1I GRCGYC 2 cut(s) 5, 513
Hin1II CATG 2 cut(s) 469, 643
HindIII AAGCTT 2 cut(s) 83, 697
HinfI GANTC 4 cut(s) 118, 500, 546, 603
HpaII CCGG 1 cut(s) 436
HphI GGTGA 2 cut(s) 152, 388
Hpy188I TCNGA 4 cut(s) 42, 376, 499, 704
Hpy188III TCNNGA 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 518
HpyAV CCTTC 3 cut(s) 305, 348, 634
HpyCH4III ACNGT 5 cut(s) 92, 146, 400, 485, 557
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 7 cut(s) 31, 56, 186, 271, 465, 634, 694
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 519
HpyF3I CTNAG 2 cut(s) 179, 508
HpySE526I ACGT 1 cut(s) 323
Hsp92I GRCGYC 2 cut(s) 5, 513
Hsp92II CATG 2 cut(s) 469, 643
LmnI GCTCC 1 cut(s) 364
Lsp1109I GCAGC 3 cut(s) 9, 54, 255
LweI GCATC 1 cut(s) 9
MaeI CTAG 1 cut(s) 540
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 3 cut(s) 140, 476, 617
MboII GAAGA 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 5 cut(s) 187, 237, 273, 389, 586
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 2 cut(s) 494, 540
MnlI CCTC 5 cut(s) 84, 188, 213, 301, 441
Mox20I TGGCCA 1 cut(s) 644
MscI TGGCCA 1 cut(s) 644
MseI TTAA 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 644
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 678
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 2 cut(s) 28, 519
NciI CCSGG 1 cut(s) 437
NlaIII CATG 2 cut(s) 469, 643
NlaIV GGNNCC 1 cut(s) 452
NmuCI GTSAC 2 cut(s) 140, 476
NspI RCATGY 1 cut(s) 469
NspV TTCGAA 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PctI GAATGC 1 cut(s) 678
PfeI GAWTC 2 cut(s) 118, 603
PkrI GCNGC 3 cut(s) 24, 69, 270
PleI GAGTC 2 cut(s) 494, 540
PpsI GAGTC 2 cut(s) 494, 540
PsiI TTATAA 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 452
RsaI GTAC 2 cut(s) 326, 559
RsaNI GTAC 2 cut(s) 325, 558
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 3 cut(s) 23, 68, 269
SchI GAGTC 2 cut(s) 494, 540
ScrFI CCNGG 1 cut(s) 437
SfaNI GCATC 1 cut(s) 9
SfcI CTRYAG 1 cut(s) 613
SfuI TTCGAA 1 cut(s) 682
SmlI CTYRAG 1 cut(s) 75
SmoI CTYRAG 1 cut(s) 75
Sse9I AATT 5 cut(s) 187, 237, 273, 389, 586
SsiI CCGC 4 cut(s) 105, 257, 346, 543
SspMI CTAG 1 cut(s) 540
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 5 cut(s) 92, 146, 400, 485, 557
TaiI ACGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 169, 682
TasI AATT 5 cut(s) 187, 237, 273, 389, 586
TfiI GAWTC 2 cut(s) 118, 603
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 696
TseFI GTSAC 2 cut(s) 140, 476
TseI GCWGC 3 cut(s) 22, 67, 268
Tsp45I GTSAC 2 cut(s) 140, 476
TspDTI ATGAA 2 cut(s) 274, 616
TspRI CASTG 1 cut(s) 696
XagI CCTNNNNNAGG 1 cut(s) 13
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XspI CTAG 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.