RLG00000032284

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019818
Physical Location & Seq
Reverse (-)
13070465 .. 13071252
788 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032284

Sequence Viewer

Length: 636 bp
ATGGCCTTACATGGGAGGCCGCTTATTATGCAGCTGATCTCATTGGTAATGGTATCTATAGAAGCAGCATCTGATCAAGCTTTGCCTCCTCAAGCCCTGCCTGGATGCCCCGGCCGGTGTGGTAATCTCAACATCCCATATCCCTTCGGGATCGGGAAAGGTTGTTACCTAAGAGCAAGATTTAACATCACTTGCAACGAATCTGCCCAACCCGCAACAGCCCTATGGGGGGATGGCCGTAGTGTCACTACCACCAACATATTCCTCGCTGAAGGTGAGTTGCAAATATTGAGTTACATAGCCCATGATTGTTATAACAAACAGGGTAATCAAACGCAGCACGGTTCCTCTTCGCTCGGGCTGGCTCCTCCGTTCACCATCTCCAGTACCCGAAACAAGTTCACCGCCGTTGGCTGTGATACTATTGCAATTTTCAGAGGGAGTCGCCCTTACCCTAATGACGGTGGGTACATAAGGGCGTGCACGTCCATGTGTAATAATCTTGATAGTGCTGTTACCAACTCGTGCTCCGCTGGCGTTGTCCTTGGGTGTTGCCAAACTGTAATCCCTAGCGGACTCAAAAATGGTAGTGTCACTTTGAGTAGTGTTTCTCAGCATAAGGAGGCGTCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

212

Amino Acids

22.2

Weight (kDa)

8.31

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 96 1.5e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019818)

Species Orthologous Gene IDs
pyrus_communis pycom05g25280
rosa_chinensis RchiOBHm_Chr5g0016941
rosa_laevigata RLG00000032284
rosa_multiflora Rmu_sc0003630.1_g000063 Rmu_sc0003801.1_g000024 Rmu_sc0007023.1_g000008
rosa_rugosa Rorug05G0587100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 315
AciI CCGC 5 cut(s) 20, 213, 405, 531, 573
AclWI GGATC 1 cut(s) 158
AcoI YGGCCR 2 cut(s) 112, 235
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 626
AfaI GTAC 2 cut(s) 388, 470
AfiI CCNNNNNNNGG 4 cut(s) 12, 228, 229, 461
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 2 cut(s) 34, 80
AluI AGCT 2 cut(s) 34, 80
Alw21I GWGCWC 2 cut(s) 485, 530
Alw44I GTGCAC 1 cut(s) 481
AlwI GGATC 1 cut(s) 158
AlwNI CAGNNNCTG 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 356
AoxI GGCC 4 cut(s) 3, 17, 112, 235
ApaLI GTGCAC 1 cut(s) 481
ApeKI GCWGC 3 cut(s) 31, 65, 337
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 3 cut(s) 287, 367, 394
AvaI CYCGRG 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 485
BauI CACGAG 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 485, 530
BbvI GCAGC 3 cut(s) 43, 77, 349
BccI CCATC 2 cut(s) 227, 386
BceAI ACGGC 2 cut(s) 222, 392
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 1 cut(s) 73
BcnI CCSGG 1 cut(s) 111
BfaI CTAG 2 cut(s) 570, 634
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 4 cut(s) 20, 32, 66, 338
BlsI GCNGC 4 cut(s) 21, 33, 67, 339
Bme1390I CCNGG 2 cut(s) 102, 111
BmeT110I CYCGRG 1 cut(s) 356
BmgBI CACGTC 1 cut(s) 486
BmiI GGNNCC 2 cut(s) 346, 366
BmrFI CCNGG 2 cut(s) 102, 111
BmsI GCATC 2 cut(s) 77, 95
BpmI CTGGAG 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 626
BsaJI CCNNGG 2 cut(s) 109, 544
BsaXI ACNNNNNCTCC 2 cut(s) 512, 542
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 228, 229, 461
Bse118I RCCGGY 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 384
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 109, 544
BseGI GGATG 3 cut(s) 110, 132, 238
BseLI CCNNNNNNNGG 4 cut(s) 12, 228, 229, 461
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 1 cut(s) 384
BseRI GAGGAG 2 cut(s) 78, 357
BseSI GKGCMC 1 cut(s) 485
BseX3I CGGCCG 1 cut(s) 112
BseXI GCAGC 3 cut(s) 43, 77, 349
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 4 cut(s) 5, 19, 114, 237
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 2 cut(s) 485, 530
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 2 cut(s) 111, 115
BslI CCNNNNNNNGG 4 cut(s) 12, 228, 229, 461
BsnI GGCC 4 cut(s) 5, 19, 114, 237
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 2 cut(s) 485, 530
Bsp143I GATC 3 cut(s) 36, 73, 150
BspACI CCGC 5 cut(s) 20, 213, 405, 531, 573
BspANI GGCC 4 cut(s) 5, 19, 114, 237
BspCNI CTCAG 1 cut(s) 625
BspLI GGNNCC 2 cut(s) 346, 366
BspPI GGATC 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 114
BsrI ACTGG 1 cut(s) 384
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 2 cut(s) 109, 544
BssMI GATC 3 cut(s) 36, 73, 150
BssNI GRCGYC 1 cut(s) 626
BssSI CACGAG 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 544
Bst2BI CACGAG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 3 cut(s) 344, 464, 562
Bst6I CTCTTC 1 cut(s) 355
BstACI GRCGYC 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 363, 481, 535
BstDEI CTNAG 2 cut(s) 170, 612
BstF5I GGATG 3 cut(s) 110, 132, 238
BstKTI GATC 3 cut(s) 39, 76, 153
BstMBI GATC 3 cut(s) 36, 73, 150
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 28, 212, 534
BstNI CCWGG 1 cut(s) 102
BstSCI CCNGG 2 cut(s) 100, 109
BstSFI CTRYAG 1 cut(s) 57
BstSLI GKGCMC 1 cut(s) 485
BstV1I GCAGC 3 cut(s) 43, 77, 349
BstZI CGGCCG 1 cut(s) 112
BsuRI GGCC 4 cut(s) 5, 19, 114, 237
BtrI CACGTC 1 cut(s) 486
BtsCI GGATG 3 cut(s) 110, 132, 238
Cac8I GCNNGC 3 cut(s) 363, 481, 535
CaiI CAGNNNCTG 1 cut(s) 71
Cfr10I RCCGGY 1 cut(s) 114
CseI GACGC 1 cut(s) 615
Csp6I GTAC 2 cut(s) 387, 469
CviAII CATG 3 cut(s) 11, 305, 490
CviQI GTAC 2 cut(s) 387, 469
DdeI CTNAG 2 cut(s) 170, 612
DpnI GATC 3 cut(s) 38, 75, 152
DpnII GATC 3 cut(s) 36, 73, 150
EaeI YGGCCR 2 cut(s) 112, 235
EagI CGGCCG 1 cut(s) 112
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
EclXI CGGCCG 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 544
Eco52I CGGCCG 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 356
EcoRII CCWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 544
FaeI CATG 3 cut(s) 14, 308, 493
FatI CATG 3 cut(s) 10, 304, 489
FauI CCCGC 1 cut(s) 220
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 4 cut(s) 20, 32, 66, 338
FokI GGATG 3 cut(s) 117, 119, 245
Fsp4HI GCNGC 4 cut(s) 20, 32, 66, 338
FspBI CTAG 2 cut(s) 570, 634
GluI GCNGC 4 cut(s) 20, 32, 66, 338
GsuI CTGGAG 1 cut(s) 367
HaeIII GGCC 4 cut(s) 5, 19, 114, 237
HapII CCGG 2 cut(s) 111, 115
HgaI GACGC 1 cut(s) 615
Hin1I GRCGYC 1 cut(s) 626
Hin1II CATG 3 cut(s) 14, 308, 493
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 3 cut(s) 200, 442, 576
HpaII CCGG 2 cut(s) 111, 115
HphI GGTGA 3 cut(s) 287, 367, 394
Hpy166II GTNNAC 3 cut(s) 375, 402, 483
Hpy188I TCNGA 2 cut(s) 73, 437
Hpy188III TCNNGA 3 cut(s) 148, 154, 503
Hpy8I GTNNAC 3 cut(s) 375, 402, 483
HpyAV CCTTC 2 cut(s) 154, 266
HpyCH4III ACNGT 3 cut(s) 344, 464, 562
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 5 cut(s) 31, 195, 283, 428, 483
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 212, 534
HpyF3I CTNAG 2 cut(s) 170, 612
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 626
Hsp92II CATG 3 cut(s) 14, 308, 493
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 36, 73, 150
LmnI GCTCC 2 cut(s) 370, 533
LpnPI CCDG 9 cut(s) 87, 110, 114, 124, 128, 308, 347, 397, 519
Lsp1109I GCAGC 3 cut(s) 43, 77, 349
LweI GCATC 2 cut(s) 77, 95
MaeI CTAG 2 cut(s) 570, 634
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 5 cut(s) 164, 244, 293, 514, 592
MalI GATC 3 cut(s) 38, 75, 152
MboI GATC 3 cut(s) 36, 73, 150
MboII GAAGA 1 cut(s) 342
MhlI GDGCHC 2 cut(s) 485, 530
MluCI AATT 1 cut(s) 429
MlyI GAGTC 2 cut(s) 451, 570
MnlI CCTC 8 cut(s) 9, 96, 99, 275, 358, 378, 431, 616
MseI TTAA 1 cut(s) 183
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 2 cut(s) 34, 533
MspI CCGG 2 cut(s) 111, 115
MspR9I CCNGG 2 cut(s) 102, 111
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 28, 212, 534
NciI CCSGG 1 cut(s) 111
NdeII GATC 3 cut(s) 36, 73, 150
NlaIII CATG 3 cut(s) 14, 308, 493
NlaIV GGNNCC 2 cut(s) 346, 366
NmuCI GTSAC 2 cut(s) 244, 592
PfeI GAWTC 1 cut(s) 200
PkrI GCNGC 4 cut(s) 21, 33, 67, 339
PleI GAGTC 2 cut(s) 450, 570
PpsI GAGTC 2 cut(s) 450, 570
PsiI TTATAA 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 2 cut(s) 346, 366
PstNI CAGNNNCTG 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 2 cut(s) 388, 470
RsaNI GTAC 2 cut(s) 387, 469
RseI CAYNNNNRTG 1 cut(s) 488
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 4 cut(s) 20, 32, 66, 338
Sau3AI GATC 3 cut(s) 36, 73, 150
SchI GAGTC 2 cut(s) 451, 570
ScrFI CCNGG 2 cut(s) 102, 111
SduI GDGCHC 2 cut(s) 485, 530
SetI ASST 6 cut(s) 36, 82, 163, 171, 277, 488
SfaNI GCATC 2 cut(s) 77, 95
SfcI CTRYAG 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 1 cut(s) 429
SsiI CCGC 5 cut(s) 20, 213, 405, 531, 573
SspI AATATT 1 cut(s) 288
SspMI CTAG 2 cut(s) 570, 634
StyD4I CCNGG 2 cut(s) 100, 109
StyI CCWWGG 1 cut(s) 544
TaaI ACNGT 3 cut(s) 344, 464, 562
TaiI ACGT 1 cut(s) 488
TasI AATT 1 cut(s) 429
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 2 cut(s) 244, 592
TseI GCWGC 3 cut(s) 31, 65, 337
Tsp45I GTSAC 2 cut(s) 244, 592
TspGWI ACGGA 1 cut(s) 360
VneI GTGCAC 1 cut(s) 481
XspI CTAG 2 cut(s) 570, 634
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.