pycom05g29920

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011534
Physical Location & Seq
Forward (+)
30236274 .. 30239676
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1917 bp
ATGCAGACGAGGTACATGGAGAGATCGAATAGCATGGCGAGGGAGAAGCGAAGTTTGGATTCGTCTTCAGCTGAAGAAGGCCAGCCGGATAGGAAACGGCCTGCTCTCGCCAGTGTAATTGTTGAAGCTCTCAAGGTGGATAGTCTGCAGAAGCTTTGCTCGTCACTGGAGCCGATTCTACGCAGAGTTGTTAGTGAAGAGGTGGAGCGTGCTTTAGCAAAATTAGGCCCTGCCAAACTTACTGGAAGCAGGTCTTCTCCTAAACAAATTGAAGGGCCCGATGGAAGAAACTTGCAGCTGCACTTTAGGTCCAGGTTGTCCCTTCCTCTTTTTACTGGTGGAAAAGTAGAAGGGGAGTGGGGTTCTGCAATCCCTATTGTCTTGATTGATGCAAATACAAAGAATGTTGTGACATCAGGCCCGGAGTCAGCAGTGAAACTTGACGTTGTTGTGCTTGAAGGTGATTTTAACAACGAGGATGATGAGAACTGGACTGAAGAAGAATTTGAGAGCCATGTAGTAAAAGAGCGTGAAGGAAAGAGGCCACTTCTTACTGGCGATCTGCAAGTGACACTGAAGGAAGGTGTAGGAACACTAGGGGAATTGACATTTACCGATAATTCTAGCTGGATTAGGAGCAGGAAGTTTAGGCTAGGACTGAAAGTTGCCTCTGGCTTTTGCGACAACATTCGTATACGCGAAGCAAAAACAGATGCCTTTACTGTTAAGGATCACCGTGGGGAATTATACAAGAAACATTACCCTCCTGCGTTAAATGATGAGGTCTGGAGATTGGAGAAAATTGGAAAAGATGGCTCATTCCACAAGAGACTGAATAAAGCTGGAATTGTTACAGTTGAAGAGTTCCTGCGACTTGTGGTTAGAGACTCGCAGAGATTGCGGAATATTCTTGGAAGTGGCATGTCGAATAAGATGTGGGATGTACTCATACAGCATGCAAAAACTTGTCTTCTTGGCGGGAAACTCTATGTCTACTATCCTGAGGATGCAAGGAACGTTGGTGTTGTTTTTAACAATATCTACGAGTTGAGTGGCCTAATTACCAATGAACAATTTTACTCAGCTGATTCTCTCTCGGACGATCAGAAGGTCTACGTGGATGGTTTGGTAACAAAGGCATATGAGAACTGGATGCATGTTATGGAGTACGATGGAAAGTCTCTTCTTAAACAGCAGAAGAGTCCTGATGTTTCTCTACCTGAGGTCCCAATAGCCTCGCAGGATTATCCAAACTCATTTGATCAGCAGTTCACTCTACCTAGCCTGCCAGCTTCAGTTTCTTCAGAACCGCCTACTATGGATTCTGGCCTAAATGTGGGAGGTTATACTGACGGCATGGCTAACAGATTCTCGGTACAGTCACAGAATCTAAATCTCAGTGCTCCCAGTCAGCTCGATGGCTTGTCATTCCCTCTACAAAATCAGCAGCCTATCACTTCACACCAGGCTCAGTTTCAAGGAAATGAAAATATGCTTGCCCTTGGTCCACCACAGTCGTCCACATCTGGGTTCCAGAATATTGGTACATCCAATCCATCTTATAGGGGAGCCGAGGACCTCTTCCCAGAGGAAGAAATTCGTACGAGGAGCCACGAGATTCTCGAAAATGAAGATATGCAGCATCTACTTCGTATCTTTAACATGGGCGGTCAGGGTCAGGGTCAAGGCCACGGTTATGGTCATGCCTCCATGAACCTCACTGAAGACAATTATCCATATCCGTCACCATACATGCCCACTCCACAAGTGAACTACAGTGTTGATGATCAGAGCCGTTCGTCTGGGAAAGCTGTTGTTGGCTGGCTCAAGCTCAAGGCAGCCCTTAGATGGGGCATATTCATAAGAAAGAAGGCTGCTGAGAGACGGGCACAGCTTGTTGAGTTGGATGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

639

Amino Acids

71.22

Weight (kDa)

5.71

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 98 - 245 1.1e-62 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 257 - 323 7.1e-33 Calmodulin binding protein central domain
Calmod_bind_C PF20452 328 - 390 1.4e-22 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 240
AccI GTMKAC 3 cut(s) 694, 993, 1113
AccII CGCG 1 cut(s) 699
AciI CCGC 4 cut(s) 901, 978, 1310, 1668
AclI AACGTT 1 cut(s) 1017
AclWI GGATC 1 cut(s) 738
AcsI RAATTY 2 cut(s) 503, 1596
AcuI CTGAAG 7 cut(s) 51, 93, 516, 596, 1278, 1287, 1743
AfaI GTAC 6 cut(s) 14, 945, 1169, 1377, 1546, 1603
AfiI CCNNNNNNNGG 4 cut(s) 1336, 1527, 1848, 1849
AgsI TTSAA 5 cut(s) 125, 272, 458, 860, 1478
AjnI CCWGG 2 cut(s) 311, 1464
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
Alw21I GWGCWC 1 cut(s) 1405
Alw26I GTCTC 4 cut(s) 823, 879, 1185, 1876
AlwI GGATC 1 cut(s) 738
AoxI GGCC 9 cut(s) 79, 98, 226, 275, 418, 542, 1054, 1327, 1687
ApaI GGGCCC 1 cut(s) 279
ApeKI GCWGC 6 cut(s) 295, 298, 1447, 1639, 1838, 1874
ApoI RAATTY 2 cut(s) 503, 1596
Asp700I GAANNNNTTC 1 cut(s) 1596
AspS9I GGNCC 8 cut(s) 227, 275, 276, 309, 419, 1225, 1505, 1576
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 3 cut(s) 473, 725, 1737
AvaII GGWCC 4 cut(s) 309, 1225, 1505, 1576
AxyI CCTNAGG 2 cut(s) 1002, 1221
BaeGI GKGCMC 2 cut(s) 279, 1891
BanII GRGCYC 1 cut(s) 279
BauI CACGAG 1 cut(s) 1613
BbsI GAAGAC 4 cut(s) 57, 246, 962, 1731
Bbv12I GWGCWC 1 cut(s) 1405
BbvI GCAGC 6 cut(s) 285, 307, 1459, 1651, 1850, 1861
BccI CCATC 7 cut(s) 275, 806, 1115, 1166, 1412, 1564, 1842
BceAI ACGGC 3 cut(s) 113, 1369, 1779
BcgI CGANNNNNNTGC 2 cut(s) 1631, 1665
BciT130I CCWGG 2 cut(s) 313, 1466
BclI TGATCA 2 cut(s) 1261, 1786
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 4 cut(s) 823, 879, 1185, 1876
BfaI CTAG 4 cut(s) 596, 624, 653, 1281
BfmI CTRYAG 2 cut(s) 146, 1774
BfuAI ACCTGC 1 cut(s) 240
BisI GCNGC 6 cut(s) 296, 299, 1448, 1640, 1839, 1875
BlsI GCNGC 6 cut(s) 297, 300, 1449, 1641, 1840, 1876
Bme1390I CCNGG 3 cut(s) 313, 422, 1466
Bme18I GGWCC 4 cut(s) 309, 1225, 1505, 1576
BmgT120I GGNCC 8 cut(s) 227, 275, 276, 309, 419, 1225, 1505, 1576
BmiI GGNNCC 6 cut(s) 171, 277, 1227, 1532, 1570, 1610
BmrFI CCNGG 3 cut(s) 313, 422, 1466
BmrI ACTGGG 1 cut(s) 1401
BmsI GCATC 5 cut(s) 379, 703, 997, 1143, 1651
BmuI ACTGGG 1 cut(s) 1401
BpiI GAAGAC 4 cut(s) 57, 246, 962, 1731
BpmI CTGGAG 2 cut(s) 188, 808
BpuEI CTTGAG 3 cut(s) 116, 1811, 1817
BpuMI CCSGG 1 cut(s) 422
BsaAI YACGTR 1 cut(s) 1117
BsaJI CCNNGG 4 cut(s) 736, 1501, 1572, 1690
Bsc4I CCNNNNNNNGG 4 cut(s) 1336, 1527, 1848, 1849
Bse1I ACTGG 8 cut(s) 111, 171, 247, 340, 494, 559, 1154, 1407
Bse21I CCTNAGG 2 cut(s) 1002, 1221
BseBI CCWGG 2 cut(s) 313, 1466
BseDI CCNNGG 4 cut(s) 736, 1501, 1572, 1690
BseGI GGATG 7 cut(s) 484, 946, 1012, 1126, 1158, 1547, 1912
BseLI CCNNNNNNNGG 4 cut(s) 1336, 1527, 1848, 1849
BseMII CTCAG 6 cut(s) 993, 1095, 1212, 1411, 1484, 1869
BseNI ACTGG 8 cut(s) 111, 171, 247, 340, 494, 559, 1154, 1407
BseRI GAGGAG 1 cut(s) 1621
BseSI GKGCMC 2 cut(s) 279, 1891
BseXI GCAGC 6 cut(s) 285, 307, 1459, 1651, 1850, 1861
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 699
BshFI GGCC 9 cut(s) 81, 100, 228, 277, 420, 544, 1056, 1329, 1689
BsiHKAI GWGCWC 1 cut(s) 1405
BsiSI CCGG 2 cut(s) 86, 422
BsiWI CGTACG 1 cut(s) 1601
BslFI GGGAC 2 cut(s) 304, 1211
BslI CCNNNNNNNGG 4 cut(s) 1336, 1527, 1848, 1849
BsmAI GTCTC 4 cut(s) 823, 879, 1185, 1876
BsmBI CGTCTC 1 cut(s) 1876
BsmFI GGGAC 2 cut(s) 304, 1211
BsnI GGCC 9 cut(s) 81, 100, 228, 277, 420, 544, 1056, 1329, 1689
Bsp120I GGGCCC 1 cut(s) 275
Bsp1286I GDGCHC 3 cut(s) 279, 1405, 1891
Bsp143I GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
BspACI CCGC 4 cut(s) 901, 978, 1310, 1668
BspANI GGCC 9 cut(s) 81, 100, 228, 277, 420, 544, 1056, 1329, 1689
BspCNI CTCAG 6 cut(s) 994, 1094, 1213, 1410, 1483, 1870
BspFNI CGCG 1 cut(s) 699
BspLI GGNNCC 6 cut(s) 171, 277, 1227, 1532, 1570, 1610
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 240
BspPI GGATC 1 cut(s) 738
BsrI ACTGG 8 cut(s) 111, 171, 247, 340, 494, 559, 1154, 1407
BssECI CCNNGG 4 cut(s) 736, 1501, 1572, 1690
BssMI GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
BssNAI GTATAC 1 cut(s) 695
BssSI CACGAG 1 cut(s) 1613
BssT1I CCWWGG 1 cut(s) 1501
Bst1107I GTATAC 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 1613
Bst2UI CCWGG 2 cut(s) 313, 1466
Bst4CI ACNGT 7 cut(s) 724, 737, 856, 1380, 1515, 1694, 1778
Bst6I CTCTTC 5 cut(s) 192, 855, 1188, 1193, 1586
BstAPI GCANNNNNTGC 1 cut(s) 898
BstBAI YACGTR 1 cut(s) 1117
BstC8I GCNNGC 8 cut(s) 83, 102, 210, 957, 1286, 1290, 1497, 1823
BstDEI CTNAG 7 cut(s) 1002, 1081, 1221, 1397, 1470, 1844, 1878
BstDSI CCRYGG 2 cut(s) 736, 1690
BstF5I GGATG 7 cut(s) 484, 946, 1012, 1126, 1158, 1547, 1912
BstFNI CGCG 1 cut(s) 699
BstKTI GATC 6 cut(s) 26, 562, 733, 1105, 1264, 1789
BstMAI GTCTC 4 cut(s) 823, 879, 1185, 1876
BstMBI GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
BstMWI GCNNNNNNNGC 1 cut(s) 898
BstNI CCWGG 2 cut(s) 313, 1466
BstNSI RCATGY 4 cut(s) 925, 959, 1160, 1756
BstSCI CCNGG 3 cut(s) 311, 420, 1464
BstSFI CTRYAG 2 cut(s) 146, 1774
BstSLI GKGCMC 2 cut(s) 279, 1891
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 6 cut(s) 285, 307, 1459, 1651, 1850, 1861
BstV2I GAAGAC 4 cut(s) 57, 246, 962, 1731
BstXI CCANNNNNNTGG 2 cut(s) 1541, 1697
BstZ17I GTATAC 1 cut(s) 695
Bsu36I CCTNAGG 2 cut(s) 1002, 1221
BsuRI GGCC 9 cut(s) 81, 100, 228, 277, 420, 544, 1056, 1329, 1689
BtgI CCRYGG 2 cut(s) 736, 1690
BtsCI GGATG 7 cut(s) 484, 946, 1012, 1126, 1158, 1547, 1912
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 7 cut(s) 118, 164, 438, 572, 1405, 1719, 1783
BveI ACCTGC 1 cut(s) 240
Cac8I GCNNGC 8 cut(s) 83, 102, 210, 957, 1286, 1290, 1497, 1823
Cfr13I GGNCC 8 cut(s) 227, 275, 276, 309, 419, 1225, 1505, 1576
Csp6I GTAC 6 cut(s) 13, 944, 1168, 1376, 1545, 1602
CviQI GTAC 6 cut(s) 13, 944, 1168, 1376, 1545, 1602
DdeI CTNAG 7 cut(s) 1002, 1081, 1221, 1397, 1470, 1844, 1878
DpnI GATC 6 cut(s) 25, 561, 732, 1104, 1263, 1788
DpnII GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
Eam1104I CTCTTC 5 cut(s) 192, 855, 1188, 1193, 1586
EarI CTCTTC 5 cut(s) 192, 855, 1188, 1193, 1586
Eco130I CCWWGG 1 cut(s) 1501
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 4 cut(s) 309, 1225, 1505, 1576
Eco57I CTGAAG 7 cut(s) 51, 93, 516, 596, 1278, 1287, 1743
Eco81I CCTNAGG 2 cut(s) 1002, 1221
EcoO109I RGGNCCY 4 cut(s) 227, 275, 1225, 1576
EcoRII CCWGG 2 cut(s) 311, 1464
EcoT14I CCWWGG 1 cut(s) 1501
EcoT22I ATGCAT 1 cut(s) 1158
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 1501
Esp3I CGTCTC 1 cut(s) 1876
FalI AAGNNNNNCTT 2 cut(s) 238, 270
FaqI GGGAC 2 cut(s) 304, 1211
FauI CCCGC 1 cut(s) 971
FauNDI CATATG 1 cut(s) 1141
FbaI TGATCA 2 cut(s) 1261, 1786
FblI GTMKAC 3 cut(s) 694, 993, 1113
Fnu4HI GCNGC 6 cut(s) 296, 299, 1448, 1640, 1839, 1875
FokI GGATG 6 cut(s) 491, 953, 1019, 1133, 1165, 1534
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 6 cut(s) 296, 299, 1448, 1640, 1839, 1875
FspBI CTAG 4 cut(s) 596, 624, 653, 1281
GluI GCNGC 6 cut(s) 296, 299, 1448, 1640, 1839, 1875
GsuI CTGGAG 2 cut(s) 188, 808
HaeIII GGCC 9 cut(s) 81, 100, 228, 277, 420, 544, 1056, 1329, 1689
HapII CCGG 2 cut(s) 86, 422
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 2 cut(s) 86, 422
HphI GGTGA 3 cut(s) 473, 725, 1737
Hpy166II GTNNAC 7 cut(s) 695, 994, 1114, 1272, 1508, 1521, 1771
Hpy188I TCNGA 4 cut(s) 1099, 1107, 1306, 1791
Hpy188III TCNNGA 6 cut(s) 382, 787, 1001, 1205, 1534, 1622
Hpy8I GTNNAC 7 cut(s) 695, 994, 1114, 1272, 1508, 1521, 1771
HpyCH4III ACNGT 7 cut(s) 724, 737, 856, 1380, 1515, 1694, 1778
HpyCH4IV ACGT 3 cut(s) 444, 1017, 1116
HpyF10VI GCNNNNNNNGC 1 cut(s) 898
HpyF3I CTNAG 7 cut(s) 1002, 1081, 1221, 1397, 1470, 1844, 1878
HpySE526I ACGT 3 cut(s) 444, 1017, 1116
Ksp22I TGATCA 2 cut(s) 1261, 1786
Kzo9I GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
LmnI GCTCC 6 cut(s) 169, 205, 636, 1408, 1568, 1608
Lsp1109I GCAGC 6 cut(s) 285, 307, 1459, 1651, 1850, 1861
LweI GCATC 5 cut(s) 379, 703, 997, 1143, 1651
MaeI CTAG 4 cut(s) 596, 624, 653, 1281
MaeII ACGT 3 cut(s) 444, 1017, 1116
MaeIII GTNAC 7 cut(s) 162, 409, 568, 850, 1129, 1380, 1743
MalI GATC 6 cut(s) 25, 561, 732, 1104, 1263, 1788
MboI GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
MhlI GDGCHC 3 cut(s) 279, 1405, 1891
MlyI GAGTC 3 cut(s) 434, 881, 1210
MmeI TCCRAC 1 cut(s) 1884
Mph1103I ATGCAT 1 cut(s) 1158
MroXI GAANNNNTTC 1 cut(s) 1596
MseI TTAA 6 cut(s) 468, 726, 773, 1032, 1188, 1659
MslI CAYNNNNRTG 1 cut(s) 1695
MspA1I CMGCKG 3 cut(s) 71, 298, 1085
MspI CCGG 2 cut(s) 86, 422
MspR9I CCNGG 3 cut(s) 313, 422, 1466
MvaI CCWGG 2 cut(s) 313, 1466
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 1 cut(s) 898
NciI CCSGG 1 cut(s) 422
NdeI CATATG 1 cut(s) 1141
NdeII GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
NlaIV GGNNCC 6 cut(s) 171, 277, 1227, 1532, 1570, 1610
NmeAIII GCCGAG 1 cut(s) 1597
NmuCI GTSAC 5 cut(s) 162, 409, 568, 1380, 1743
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 4 cut(s) 925, 959, 1160, 1756
PaeI GCATGC 1 cut(s) 959
PcsI WCGNNNNNNNCGW 1 cut(s) 1620
PdmI GAANNNNTTC 1 cut(s) 1596
PfeI GAWTC 8 cut(s) 59, 175, 1088, 1322, 1368, 1387, 1618, 1910
Pfl23II CGTACG 1 cut(s) 1601
PkrI GCNGC 6 cut(s) 297, 300, 1449, 1641, 1840, 1876
PleI GAGTC 3 cut(s) 433, 881, 1209
PpsI GAGTC 3 cut(s) 433, 881, 1209
Ppu21I YACGTR 1 cut(s) 1117
PpuMI RGGWCCY 2 cut(s) 1225, 1576
Psp1406I AACGTT 1 cut(s) 1017
Psp5II RGGWCCY 2 cut(s) 1225, 1576
Psp6I CCWGG 2 cut(s) 311, 1464
PspGI CCWGG 2 cut(s) 311, 1464
PspLI CGTACG 1 cut(s) 1601
PspN4I GGNNCC 6 cut(s) 171, 277, 1227, 1532, 1570, 1610
PspOMI GGGCCC 1 cut(s) 275
PspPI GGNCC 8 cut(s) 227, 275, 276, 309, 419, 1225, 1505, 1576
PspPPI RGGWCCY 2 cut(s) 1225, 1576
PstI CTGCAG 1 cut(s) 150
PvuII CAGCTG 3 cut(s) 71, 298, 1085
RsaI GTAC 6 cut(s) 14, 945, 1169, 1377, 1546, 1603
RsaNI GTAC 6 cut(s) 13, 944, 1168, 1376, 1545, 1602
RseI CAYNNNNRTG 1 cut(s) 1695
SaqAI TTAA 6 cut(s) 468, 726, 773, 1032, 1188, 1659
SatI GCNGC 6 cut(s) 296, 299, 1448, 1640, 1839, 1875
Sau3AI GATC 6 cut(s) 23, 559, 730, 1102, 1261, 1786
Sau96I GGNCC 8 cut(s) 227, 275, 276, 309, 419, 1225, 1505, 1576
SchI GAGTC 3 cut(s) 434, 881, 1210
ScrFI CCNGG 3 cut(s) 313, 422, 1466
SduI GDGCHC 3 cut(s) 279, 1405, 1891
SfaNI GCATC 5 cut(s) 379, 703, 997, 1143, 1651
SfcI CTRYAG 2 cut(s) 146, 1774
SinI GGWCC 4 cut(s) 309, 1225, 1505, 1576
SmiMI CAYNNNNRTG 1 cut(s) 1695
SmlI CTYRAG 3 cut(s) 131, 1826, 1832
SmoI CTYRAG 3 cut(s) 131, 1826, 1832
SphI GCATGC 1 cut(s) 959
SsiI CCGC 4 cut(s) 901, 978, 1310, 1668
SspI AATATT 2 cut(s) 907, 1540
SspMI CTAG 4 cut(s) 596, 624, 653, 1281
StyD4I CCNGG 3 cut(s) 311, 420, 1464
StyI CCWWGG 1 cut(s) 1501
TaaI ACNGT 7 cut(s) 724, 737, 856, 1380, 1515, 1694, 1778
TaiI ACGT 3 cut(s) 447, 1020, 1119
TaqI TCGA 4 cut(s) 26, 926, 1416, 1623
TatI WGTACW 1 cut(s) 943
TfiI GAWTC 8 cut(s) 59, 175, 1088, 1322, 1368, 1387, 1618, 1910
Tru1I TTAA 6 cut(s) 468, 726, 773, 1032, 1188, 1659
Tru9I TTAA 6 cut(s) 468, 726, 773, 1032, 1188, 1659
TscAI CASTG 7 cut(s) 118, 171, 438, 579, 1405, 1726, 1783
TseFI GTSAC 5 cut(s) 162, 409, 568, 1380, 1743
TseI GCWGC 6 cut(s) 295, 298, 1447, 1639, 1838, 1874
Tsp45I GTSAC 5 cut(s) 162, 409, 568, 1380, 1743
TspDTI ATGAA 6 cut(s) 1083, 1500, 1644, 1727, 1849, 1902
TspGWI ACGGA 1 cut(s) 1731
TspRI CASTG 7 cut(s) 118, 171, 438, 579, 1405, 1726, 1783
VpaK11BI GGWCC 4 cut(s) 309, 1225, 1505, 1576
XapI RAATTY 2 cut(s) 503, 1596
XceI RCATGY 4 cut(s) 925, 959, 1160, 1756
XmiI GTMKAC 3 cut(s) 694, 993, 1113
XmnI GAANNNNTTC 1 cut(s) 1596
XspI CTAG 4 cut(s) 596, 624, 653, 1281
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.