pycom05g32950

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008724
Physical Location & Seq
Forward (+)
32146318 .. 32148974
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1833 bp
ATGAAGTTTATGAAAATTGGAACCAAGCCGGACACCTTCTACGCTGAAGAAGCTACCAGGACCGTGATTTCGGATGCACCCAGCGACTTTGTCATACAAATTAACAACATCAATTATCTTCTCCATACGTTTCCACTTCTTCCAAAATGTGGCCTCTTACAACGGCTTTGCTCTCACTCTGGTGATTCGGAAAAGGTCAGCATAGAGCTTCACGATATTCCAGGAGGTGAAGATGCGTTCGAAATGTGTGCTAAGTTCTGTTACGGAATTACGATTAATCTCAGCGCCTATAACTTTGTACCTGCATTTTGTGCTGCTAAGTTCCTCCGAATGACTGAGTCAGTAGAGAAGGGAAATTTTGTTCCGAAACTTGAGGCTTTCTTCAATTCATGCATTCTTGAAGGTTGGAAAGACTCTATTACTGCACTACAAACTACAGTGAAGTTTCCTGAGTGGTCTGAGAATCTTGGGATCATCAGAAAGTGCATTGATTCAATTGTTGAGAAAATCCATACTCCTCCAGCAAAGGTTTCATGGTCCTACACTTATACCAGGCCTGGCTACACCAAAAAGCAACATCATTCTGTCCCAAAGGATTGGTGGACTGAGGATATATCAGACCTTGATGTAGACCCGTTTCGATGTATAATTACTGCTGTTAGATCCACGTATATGCTGCCACCGCAGCTCATTGGGGAAGCTTTGCACGTCTATGCTTGTTGTTGGCTGCCAGACACCACAAGAACACCTCCACAAACAGGTGAGGAATTTATGGAAAAGAATCGAAGAATTGTTGATAGCATTGTGAGCATGATTCCCGAAGACAAGGGAGCAGTTTATGTTGGTTTCTTGCTAAGGCTTATTGTCATTGCCAATTACTTGGGAGTGTCCCCAGTGACAAAGACAGAATTGTTAAGGAGGTCCGGTCTACAACTCGAAGAGGCAACGGTGGATGACCTGATTTTTCCTTCACACTCTCCCGCTGACCCAGAATTTTACGATATTGACTTGGTTTTGGCAGTGTTAGAAAGTTTCTTAGTGCTATCGAGAAGACAATCCCCTGCAGCTGCTTCTGAATTGAACAGAAGCGCTCAGGTTTTAGGAACAATGAGAAAGGTTGGGAAACTCATTGATTCGTACCTTCAAGTCGTTGCCAAGGATGTCAACATGCCAGTTTCAAAAGTGGTATCACTGGCTGAAGCTTTGCCAGATATTGCAAGGGAAGACCATGACGGCATCTACAAGGCTATTAACATTTATCTGAAGGAGCATGGGGATCTGAGCAAGGCAGACAAGAAGCGCCTGTGCCGCATTTTAGACTGCCAGAAGTTGTCGCCTGAGATACGTGCTCATGCTGTGAAAAATGAGCGGCTACCATTGAGGATTGTTGTGCAAGTCCTCTTCTTTGAACAGGAGAGAGACAGAGATTCCAACTCCAAGACAGCAGCAGCAACTCCTGATCGTCGTAAACATAAACCACTGCCAGTTAAACCCTCCCAGTCCCAGGAGCCGGGGAAACAAACAGTGCCAGCTAGCAGAGAATCAAATAGTCATAGTCATGATGAGGTAAAATTTGGAGCAGCAAGTGTTCATAATAAATTCAGTAGTAGTACCACTGGAAAGAGAGATTATGCGCTGCTGCTAATGCAAAACAAAAGATCAGACGGAATGTTAGCAGTGGAAAACGAAAGAAAGGGTGTCAGATCAGAAACGCAACAAGTAGATCATCAAGGACATGATACCAAAAGGGAAATCCTAAGAGAAGGAGCATCTGGATCTGCAGGAGCAGGGAGCAAGTTGGACGCTAAGAAGATCGATCCAAAGGGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

611

Amino Acids

68.31

Weight (kDa)

7.89

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 199 - 447 1.8e-78 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 149
AccBSI CCGCTC 1 cut(s) 1369
AccI GTMKAC 2 cut(s) 630, 928
AciI CCGC 4 cut(s) 683, 981, 1309, 1369
AclWI GGATC 5 cut(s) 479, 657, 1284, 1783, 1811
AcsI RAATTY 5 cut(s) 355, 767, 992, 1571, 1598
AcuI CTGAAG 3 cut(s) 66, 1218, 1283
AfaI GTAC 3 cut(s) 300, 1139, 1612
AfeI AGCGCT 1 cut(s) 1090
AfiI CCNNNNNNNGG 4 cut(s) 149, 758, 1504, 1510
AgsI TTSAA 7 cut(s) 385, 401, 495, 1081, 1145, 1179, 1409
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 5 cut(s) 56, 220, 551, 556, 1503
AleI CACNNNNGTG 1 cut(s) 180
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 7 cut(s) 53, 208, 688, 701, 1067, 1202, 1532
AluI AGCT 7 cut(s) 53, 208, 688, 701, 1067, 1202, 1532
Alw21I GWGCWC 1 cut(s) 1351
Alw26I GTCTC 1 cut(s) 1413
AlwI GGATC 5 cut(s) 479, 657, 1284, 1783, 1811
Aor51HI AGCGCT 1 cut(s) 1090
AoxI GGCC 2 cut(s) 151, 554
ApoI RAATTY 5 cut(s) 355, 767, 992, 1571, 1598
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AseI ATTAAT 1 cut(s) 276
AspLEI GCGC 4 cut(s) 287, 1091, 1302, 1636
AspS9I GGNCC 3 cut(s) 60, 537, 921
AsuC2I CCSGG 1 cut(s) 1512
AsuHPI GGTGA 3 cut(s) 194, 239, 773
AsuII TTCGAA 1 cut(s) 240
AsuNHI GCTAGC 1 cut(s) 1532
AvaII GGWCC 3 cut(s) 60, 537, 921
BbsI GAAGAC 3 cut(s) 828, 1057, 1230
Bbv12I GWGCWC 1 cut(s) 1351
BceAI ACGGC 2 cut(s) 179, 1249
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 5 cut(s) 58, 222, 553, 558, 1505
BcnI CCSGG 1 cut(s) 1512
BcoDI GTCTC 1 cut(s) 1413
BfaI CTAG 1 cut(s) 1533
BfmI CTRYAG 3 cut(s) 435, 1062, 1779
BfoI RGCGCY 3 cut(s) 288, 1092, 1303
BfuAI ACCTGC 1 cut(s) 310
Bme1390I CCNGG 6 cut(s) 58, 222, 553, 558, 1505, 1512
Bme18I GGWCC 3 cut(s) 60, 537, 921
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 3 cut(s) 60, 537, 921
BmiI GGNNCC 2 cut(s) 22, 1509
BmrFI CCNGG 6 cut(s) 58, 222, 553, 558, 1505, 1512
BmrI ACTGGG 2 cut(s) 887, 1492
BmsI GCATC 4 cut(s) 64, 223, 1245, 1778
BmtI GCTAGC 1 cut(s) 1536
BmuI ACTGGG 2 cut(s) 887, 1492
BpiI GAAGAC 3 cut(s) 828, 1057, 1230
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 2 cut(s) 854, 1092
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 1512
Bsa29I ATCGAT 1 cut(s) 1815
BsaAI YACGTR 2 cut(s) 669, 1346
BsaJI CCNNGG 3 cut(s) 1155, 1503, 1511
BsaWI WCCGGW 1 cut(s) 923
BsaXI ACNNNNNCTCC 2 cut(s) 910, 940
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 758, 1504, 1510
Bse1I ACTGG 6 cut(s) 893, 1172, 1197, 1484, 1498, 1621
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 5 cut(s) 58, 222, 553, 558, 1505
BseCI ATCGAT 1 cut(s) 1815
BseDI CCNNGG 3 cut(s) 1155, 1503, 1511
BseGI GGATG 3 cut(s) 79, 958, 1165
BseLI CCNNNNNNNGG 4 cut(s) 149, 758, 1504, 1510
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 8 cut(s) 295, 327, 441, 450, 597, 1106, 1271, 1329
BseNI ACTGG 6 cut(s) 893, 1172, 1197, 1484, 1498, 1621
BseRI GAGGAG 1 cut(s) 507
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 408
BshFI GGCC 2 cut(s) 153, 556
BshVI ATCGAT 1 cut(s) 1815
BsiHKAI GWGCWC 1 cut(s) 1351
BsiSI CCGG 3 cut(s) 29, 924, 1511
BslFI GGGAC 3 cut(s) 572, 874, 1486
BslI CCNNNNNNNGG 4 cut(s) 149, 758, 1504, 1510
BsmAI GTCTC 1 cut(s) 1413
BsmFI GGGAC 3 cut(s) 572, 874, 1486
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 2 cut(s) 153, 556
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 1351
BspACI CCGC 4 cut(s) 683, 981, 1309, 1369
BspANI GGCC 2 cut(s) 153, 556
BspCNI CTCAG 8 cut(s) 294, 328, 442, 451, 598, 1105, 1272, 1330
BspDI ATCGAT 1 cut(s) 1815
BspHI TCATGA 1 cut(s) 1558
BspLI GGNNCC 2 cut(s) 22, 1509
BspMAI CTGCAG 2 cut(s) 1066, 1783
BspMI ACCTGC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 1536
BspPI GGATC 5 cut(s) 479, 657, 1284, 1783, 1811
BspT104I TTCGAA 1 cut(s) 240
BsrBI CCGCTC 1 cut(s) 1369
BsrDI GCAATG 1 cut(s) 867
BsrI ACTGG 6 cut(s) 893, 1172, 1197, 1484, 1498, 1621
BssECI CCNNGG 3 cut(s) 1155, 1503, 1511
BssT1I CCWWGG 1 cut(s) 1155
Bst2UI CCWGG 5 cut(s) 58, 222, 553, 558, 1505
Bst4CI ACNGT 4 cut(s) 64, 439, 949, 1525
Bst6I CTCTTC 2 cut(s) 933, 1406
BstAPI GCANNNNNTGC 1 cut(s) 311
BstBAI YACGTR 2 cut(s) 669, 1346
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 1530, 1534
BstF5I GGATG 3 cut(s) 79, 958, 1165
BstH2I RGCGCY 3 cut(s) 288, 1092, 1303
BstHHI GCGC 4 cut(s) 287, 1091, 1302, 1636
BstMAI GTCTC 1 cut(s) 1413
BstMWI GCNNNNNNNGC 7 cut(s) 50, 311, 682, 685, 807, 1308, 1645
BstNI CCWGG 5 cut(s) 58, 222, 553, 558, 1505
BstNSI RCATGY 1 cut(s) 1171
BstSCI CCNGG 6 cut(s) 56, 220, 551, 556, 1503, 1510
BstSFI CTRYAG 3 cut(s) 435, 1062, 1779
BstV2I GAAGAC 3 cut(s) 828, 1057, 1230
BstX2I RGATCY 3 cut(s) 662, 1276, 1775
BstXI CCANNNNNNTGG 2 cut(s) 597, 880
BstYI RGATCY 3 cut(s) 662, 1276, 1775
Bsu15I ATCGAT 1 cut(s) 1815
BsuRI GGCC 2 cut(s) 153, 556
BsuTUI ATCGAT 1 cut(s) 1815
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 3 cut(s) 79, 958, 1165
BtsI GCAGTG 3 cut(s) 1026, 1478, 1683
BtsIMutI CAGTG 8 cut(s) 444, 900, 1026, 1190, 1478, 1530, 1614, 1683
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 2 cut(s) 1530, 1534
CciI TCATGA 1 cut(s) 1558
CfoI GCGC 4 cut(s) 287, 1091, 1302, 1636
Cfr13I GGNCC 3 cut(s) 60, 537, 921
ClaI ATCGAT 1 cut(s) 1815
CseI GACGC 1 cut(s) 1811
Csp6I GTAC 3 cut(s) 299, 1138, 1611
CviAII CATG 9 cut(s) 390, 534, 811, 1168, 1229, 1271, 1352, 1559, 1736
CviQI GTAC 3 cut(s) 299, 1138, 1611
Eam1104I CTCTTC 2 cut(s) 933, 1406
EarI CTCTTC 2 cut(s) 933, 1406
Eco130I CCWWGG 1 cut(s) 1155
Eco147I AGGCCT 1 cut(s) 556
Eco47I GGWCC 3 cut(s) 60, 537, 921
Eco47III AGCGCT 1 cut(s) 1090
Eco57I CTGAAG 3 cut(s) 66, 1218, 1283
EcoRII CCWGG 5 cut(s) 56, 220, 551, 556, 1503
EcoT14I CCWWGG 1 cut(s) 1155
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 1 cut(s) 1155
FaeI CATG 9 cut(s) 393, 537, 814, 1171, 1232, 1274, 1355, 1562, 1739
FaqI GGGAC 3 cut(s) 572, 874, 1486
FatI CATG 9 cut(s) 389, 533, 810, 1167, 1228, 1270, 1351, 1558, 1735
FauI CCCGC 1 cut(s) 988
FblI GTMKAC 2 cut(s) 630, 928
FokI GGATG 3 cut(s) 86, 965, 1172
FspBI CTAG 1 cut(s) 1533
GlaI GCGC 4 cut(s) 286, 1090, 1301, 1635
GsaI CCCAGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 3 cut(s) 288, 1092, 1303
HaeIII GGCC 2 cut(s) 153, 556
HapII CCGG 3 cut(s) 29, 924, 1511
HgaI GACGC 1 cut(s) 1811
HhaI GCGC 4 cut(s) 287, 1091, 1302, 1636
Hin1II CATG 9 cut(s) 393, 537, 814, 1171, 1232, 1274, 1355, 1562, 1739
Hin6I GCGC 4 cut(s) 285, 1089, 1300, 1634
HinP1I GCGC 4 cut(s) 285, 1089, 1300, 1634
HincII GTYRAC 1 cut(s) 1165
HindII GTYRAC 1 cut(s) 1165
HindIII AAGCTT 2 cut(s) 699, 1200
HpaII CCGG 3 cut(s) 29, 924, 1511
HphI GGTGA 3 cut(s) 194, 239, 773
Hpy166II GTNNAC 5 cut(s) 603, 631, 929, 1165, 1469
Hpy188III TCNNGA 8 cut(s) 212, 398, 449, 818, 1047, 1457, 1559, 1773
Hpy8I GTNNAC 5 cut(s) 603, 631, 929, 1165, 1469
Hpy99I CGWCG 1 cut(s) 1467
HpyAV CCTTC 7 cut(s) 46, 343, 395, 978, 1151, 1258, 1757
HpyCH4III ACNGT 4 cut(s) 64, 439, 949, 1525
HpyCH4IV ACGT 4 cut(s) 128, 668, 708, 1345
HpyF10VI GCNNNNNNNGC 7 cut(s) 50, 311, 682, 685, 807, 1308, 1645
HpySE526I ACGT 4 cut(s) 128, 668, 708, 1345
Hsp92II CATG 9 cut(s) 393, 537, 814, 1171, 1232, 1274, 1355, 1562, 1739
HspAI GCGC 4 cut(s) 285, 1089, 1300, 1634
LmnI GCTCC 7 cut(s) 830, 1267, 1507, 1577, 1766, 1784, 1791
LweI GCATC 4 cut(s) 64, 223, 1245, 1778
MaeI CTAG 1 cut(s) 1533
MaeII ACGT 4 cut(s) 128, 668, 708, 1345
MaeIII GTNAC 2 cut(s) 260, 895
MbiI CCGCTC 1 cut(s) 1369
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 3 cut(s) 662, 1276, 1775
MhlI GDGCHC 1 cut(s) 1351
MlyI GAGTC 2 cut(s) 347, 407
MmeI TCCRAC 3 cut(s) 386, 1455, 1779
Mph1103I ATGCAT 1 cut(s) 395
MseI TTAA 5 cut(s) 102, 276, 914, 1251, 1488
MslI CAYNNNNRTG 4 cut(s) 180, 671, 711, 1557
MspA1I CMGCKG 2 cut(s) 983, 1067
MspI CCGG 3 cut(s) 29, 924, 1511
MspR9I CCNGG 6 cut(s) 58, 222, 553, 558, 1505, 1512
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 5 cut(s) 58, 222, 553, 558, 1505
MwoI GCNNNNNNNGC 7 cut(s) 50, 311, 682, 685, 807, 1308, 1645
NciI CCSGG 1 cut(s) 1512
NheI GCTAGC 1 cut(s) 1532
NlaIII CATG 9 cut(s) 393, 537, 814, 1171, 1232, 1274, 1355, 1562, 1739
NlaIV GGNNCC 2 cut(s) 22, 1509
NmuCI GTSAC 1 cut(s) 895
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 240
OliI CACNNNNGTG 1 cut(s) 180
PagI TCATGA 1 cut(s) 1558
PceI AGGCCT 1 cut(s) 556
PctI GAATGC 1 cut(s) 393
PfeI GAWTC 8 cut(s) 185, 463, 491, 781, 814, 1133, 1427, 1541
PflFI GACNNNGTC 2 cut(s) 89, 337
PflMI CCANNNNNTGG 1 cut(s) 149
PfoI TCCNGGA 1 cut(s) 220
PleI GAGTC 2 cut(s) 346, 407
PpsI GAGTC 2 cut(s) 346, 407
Ppu21I YACGTR 2 cut(s) 669, 1346
PshBI ATTAAT 1 cut(s) 276
Psp6I CCWGG 5 cut(s) 56, 220, 551, 556, 1503
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 5 cut(s) 56, 220, 551, 556, 1503
PspN4I GGNNCC 2 cut(s) 22, 1509
PspPI GGNCC 3 cut(s) 60, 537, 921
PstI CTGCAG 2 cut(s) 1066, 1783
PsuI RGATCY 3 cut(s) 662, 1276, 1775
PsyI GACNNNGTC 2 cut(s) 89, 337
PvuII CAGCTG 1 cut(s) 1067
RsaI GTAC 3 cut(s) 300, 1139, 1612
RsaNI GTAC 3 cut(s) 299, 1138, 1611
RseI CAYNNNNRTG 4 cut(s) 180, 671, 711, 1557
SaqAI TTAA 5 cut(s) 102, 276, 914, 1251, 1488
Sau96I GGNCC 3 cut(s) 60, 537, 921
SchI GAGTC 2 cut(s) 347, 407
ScrFI CCNGG 6 cut(s) 58, 222, 553, 558, 1505, 1512
SduI GDGCHC 1 cut(s) 1351
SfaNI GCATC 4 cut(s) 64, 223, 1245, 1778
SfcI CTRYAG 3 cut(s) 435, 1062, 1779
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 3 cut(s) 60, 537, 921
SmiMI CAYNNNNRTG 4 cut(s) 180, 671, 711, 1557
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
SseBI AGGCCT 1 cut(s) 556
SsiI CCGC 4 cut(s) 683, 981, 1309, 1369
SspMI CTAG 1 cut(s) 1533
StuI AGGCCT 1 cut(s) 556
StyD4I CCNGG 6 cut(s) 56, 220, 551, 556, 1503, 1510
StyI CCWWGG 1 cut(s) 1155
TaaI ACNGT 4 cut(s) 64, 439, 949, 1525
TaiI ACGT 4 cut(s) 131, 671, 711, 1348
TaqI TCGA 6 cut(s) 240, 640, 784, 936, 1046, 1815
TauI GCSGC 2 cut(s) 1311, 1372
TfiI GAWTC 8 cut(s) 185, 463, 491, 781, 814, 1133, 1427, 1541
Tru1I TTAA 5 cut(s) 102, 276, 914, 1251, 1488
Tru9I TTAA 5 cut(s) 102, 276, 914, 1251, 1488
TscAI CASTG 8 cut(s) 444, 900, 1026, 1197, 1485, 1530, 1621, 1683
TseFI GTSAC 1 cut(s) 895
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 5 cut(s) 17, 26, 378, 522, 1580
TspGWI ACGGA 2 cut(s) 279, 1680
TspRI CASTG 8 cut(s) 444, 900, 1026, 1197, 1485, 1530, 1621, 1683
Tth111I GACNNNGTC 2 cut(s) 89, 337
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 3 cut(s) 60, 537, 921
VspI ATTAAT 1 cut(s) 276
XapI RAATTY 5 cut(s) 355, 767, 992, 1571, 1598
XceI RCATGY 1 cut(s) 1171
XcmI CCANNNNNNNNNTGG 1 cut(s) 597
XmiI GTMKAC 2 cut(s) 630, 928
XspI CTAG 1 cut(s) 1533
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.