pycom06g06020

Pentatricopeptide repeat-containing protein At4g18975

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009535
Physical Location & Seq
Forward (+)
9267495 .. 9271259
3765 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
ATGTGGAAGTCTCCTGCCATGTCCATTCTCGTTGGGCGGTTGACTCGGCTTGGAGTTACTAGGGCCCAAATTCTGAATTCGACTTACAGTACTGTGGTGCAAGCTCAAATATCTAACCAAAGAGCTGCAAAGACTACGGTGGCCTTGGAGGATCAACACAACCACCAAGGTAATAGTTACGATCCAGAGAAGAATGCTGAAGGGGAGAAAAGGGACCAAATAGGATGGAATGTTCCTAGAAAGGATAAGGTTAACTTTCTTGTAAAAACTCTTCTAGATCTCAGTGACAGTAAGGAGGCTGTTTACGGTTCTCTTGATGGATGGGTTGCATGGGAGGAAGACTTTCCCATTGGAAAAATTAGGATGGCGTTAATTGCTCTTGAGAAGGAACAGCAGTGGCATAGAATAGTTCAGGTAATCAAATGGATGCTAAGCAAGGGACAAGGAAACACAATGGGAACATATGGGCAACTGATCCGAGCTTTAGATATGGACCAGAGACCCGAAGAAGCACACAAGTTTTGGGAGAAGAAGATTGGCATGGATTTGCATGCAGTTCCATGGAAGTTATGCAAAAGTATGATAGCTATATATTATCGAAACAACATGTTGAAGAATCTTGTAAAGCTTTTTGAGGGCCTGGAAGCTTTTGATCGGAAACCTCCAGAGAAGTCAATTGTCCGGAGAGTGGCAGATGCATATGAGATGTTAGGCATGCTTGAAGACAAAGAGAGGGTGTTACAGAAGTACAACTATCTTTTCACAGAAAATGTAGCACTCAAGAAATCCACAATGGCCTTGTATAAGAAAAAGAAGAAACACGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

31.83

Weight (kDa)

9.53

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04590 AT1G04590
fragaria_vesca FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240
malus_domestica MD06G1063200.v1.1
prunus_persica Prupe.5G075000_v2.0.a1
pyrus_communis pycom06g06020
rosa_chinensis RchiOBHm_Chr7g0202441
rosa_laevigata RLG00000003603
rosa_multiflora Rmu_sc0031703.1_g000002
rosa_roxburghii Rroxscaffold_3G00254610
rosa_rugosa Rorug07G0070600
rosa_samantha Rh7AG198000 Rh7BG199300 Rh7CG209100 Rh7DG204900
rosa_wichuraiana Rw7G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 681
AciI CCGC 1 cut(s) 37
AclWI GGATC 3 cut(s) 159, 176, 469
AcsI RAATTY 2 cut(s) 69, 76
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 2 cut(s) 91, 749
AfiI CCNNNNNNNGG 1 cut(s) 688
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 613, 722
AjnI CCWGG 1 cut(s) 639
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 6 cut(s) 104, 125, 482, 587, 628, 647
AluI AGCT 6 cut(s) 104, 125, 482, 587, 628, 647
Alw26I GTCTC 2 cut(s) 15, 493
AlwI GGATC 3 cut(s) 159, 176, 469
Aor13HI TCCGGA 1 cut(s) 681
AoxI GGCC 4 cut(s) 63, 141, 637, 795
ApaI GGGCCC 1 cut(s) 67
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 2 cut(s) 69, 76
Asp700I GAANNNNTTC 1 cut(s) 342
AspS9I GGNCC 5 cut(s) 63, 64, 214, 493, 637
AvaII GGWCC 2 cut(s) 214, 493
BaeGI GKGCMC 1 cut(s) 67
BanII GRGCYC 1 cut(s) 67
BarI GAAGNNNNNNTAC 2 cut(s) 255, 287
BbsI GAAGAC 2 cut(s) 345, 729
BbvI GCAGC 1 cut(s) 112
BccI CCATC 4 cut(s) 219, 311, 315, 358
BciT130I CCWGG 1 cut(s) 641
BcoDI GTCTC 2 cut(s) 15, 493
BfaI CTAG 3 cut(s) 60, 237, 275
BglII AGATCT 1 cut(s) 277
BisI GCNGC 1 cut(s) 126
BlpI GCTNAGC 1 cut(s) 431
BlsI GCNGC 1 cut(s) 127
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 641
Bme18I GGWCC 2 cut(s) 214, 493
BmgT120I GGNCC 5 cut(s) 63, 64, 214, 493, 637
BmiI GGNNCC 2 cut(s) 65, 215
BmrFI CCNGG 1 cut(s) 641
BmsI GCATC 2 cut(s) 417, 685
BpiI GAAGAC 2 cut(s) 345, 729
BpmI CTGGAG 1 cut(s) 648
Bpu1102I GCTNAGC 1 cut(s) 431
BpuEI CTTGAG 2 cut(s) 401, 764
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 144, 166, 560
BsaWI WCCGGW 1 cut(s) 681
Bsc4I CCNNNNNNNGG 1 cut(s) 688
BseAI TCCGGA 1 cut(s) 681
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 3 cut(s) 144, 166, 560
BseGI GGATG 4 cut(s) 230, 326, 369, 432
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 1 cut(s) 295
BseSI GKGCMC 1 cut(s) 67
BseXI GCAGC 1 cut(s) 112
BshFI GGCC 4 cut(s) 65, 143, 639, 797
BsiSI CCGG 1 cut(s) 682
BslFI GGGAC 2 cut(s) 227, 453
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 2 cut(s) 15, 493
BsmFI GGGAC 2 cut(s) 227, 453
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 4 cut(s) 65, 143, 639, 797
Bso31I GGTCTC 1 cut(s) 493
Bsp120I GGGCCC 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 67
Bsp13I TCCGGA 1 cut(s) 681
Bsp143I GATC 5 cut(s) 151, 181, 277, 474, 652
Bsp1720I GCTNAGC 1 cut(s) 431
Bsp19I CCATGG 1 cut(s) 560
BspACI CCGC 1 cut(s) 37
BspANI GGCC 4 cut(s) 65, 143, 639, 797
BspCNI CTCAG 1 cut(s) 294
BspEI TCCGGA 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 65, 215
BspPI GGATC 3 cut(s) 159, 176, 469
BspTNI GGTCTC 1 cut(s) 493
BssECI CCNNGG 3 cut(s) 144, 166, 560
BssMI GATC 5 cut(s) 151, 181, 277, 474, 652
BssT1I CCWWGG 3 cut(s) 144, 166, 560
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 6 cut(s) 89, 94, 139, 290, 308, 824
Bst6I CTCTTC 1 cut(s) 276
BstC8I GCNNGC 3 cut(s) 102, 552, 716
BstDEI CTNAG 2 cut(s) 281, 431
BstDSI CCRYGG 1 cut(s) 560
BstF5I GGATG 4 cut(s) 230, 326, 369, 432
BstKTI GATC 5 cut(s) 154, 184, 280, 477, 655
BstMAI GTCTC 2 cut(s) 15, 493
BstMBI GATC 5 cut(s) 151, 181, 277, 474, 652
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 3 cut(s) 554, 610, 718
BstSCI CCNGG 1 cut(s) 639
BstSLI GKGCMC 1 cut(s) 67
BstV1I GCAGC 1 cut(s) 112
BstV2I GAAGAC 2 cut(s) 345, 729
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 4 cut(s) 65, 143, 639, 797
BtgI CCRYGG 1 cut(s) 560
BtsCI GGATG 4 cut(s) 230, 326, 369, 432
BtsI GCAGTG 1 cut(s) 401
BtsIMutI CAGTG 2 cut(s) 289, 401
Cac8I GCNNGC 3 cut(s) 102, 552, 716
Cfr13I GGNCC 5 cut(s) 63, 64, 214, 493, 637
Csp6I GTAC 2 cut(s) 90, 748
CviAII CATG 7 cut(s) 19, 330, 541, 551, 561, 607, 715
CviQI GTAC 2 cut(s) 90, 748
DdeI CTNAG 2 cut(s) 281, 431
DpnI GATC 5 cut(s) 153, 183, 279, 476, 654
DpnII GATC 5 cut(s) 151, 181, 277, 474, 652
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco130I CCWWGG 3 cut(s) 144, 166, 560
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 493
Eco47I GGWCC 2 cut(s) 214, 493
Eco57I CTGAAG 1 cut(s) 219
EcoO109I RGGNCCY 2 cut(s) 63, 637
EcoRI GAATTC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 3 cut(s) 144, 166, 560
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 3 cut(s) 144, 166, 560
FaeI CATG 7 cut(s) 22, 333, 544, 554, 564, 610, 718
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 2 cut(s) 227, 453
FatI CATG 7 cut(s) 18, 329, 540, 550, 560, 606, 714
FauNDI CATATG 2 cut(s) 463, 700
Fnu4HI GCNGC 1 cut(s) 126
FokI GGATG 4 cut(s) 237, 333, 376, 439
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 126
FspBI CTAG 3 cut(s) 60, 237, 275
GluI GCNGC 1 cut(s) 126
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 4 cut(s) 65, 143, 639, 797
HapII CCGG 1 cut(s) 682
Hin1II CATG 7 cut(s) 22, 333, 544, 554, 564, 610, 718
HincII GTYRAC 2 cut(s) 42, 253
HindII GTYRAC 2 cut(s) 42, 253
HindIII AAGCTT 2 cut(s) 626, 645
HinfI GANTC 2 cut(s) 43, 616
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 682
Hpy166II GTNNAC 3 cut(s) 42, 253, 304
Hpy188I TCNGA 3 cut(s) 75, 479, 657
Hpy188III TCNNGA 7 cut(s) 185, 275, 314, 380, 665, 682, 781
Hpy8I GTNNAC 3 cut(s) 42, 253, 304
HpyAV CCTTC 2 cut(s) 194, 379
HpyCH4III ACNGT 6 cut(s) 89, 94, 139, 290, 308, 824
HpyCH4V TGCA 7 cut(s) 100, 128, 329, 550, 554, 573, 698
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 2 cut(s) 281, 431
Hsp92II CATG 7 cut(s) 22, 333, 544, 554, 564, 610, 718
Kpn2I TCCGGA 1 cut(s) 681
KspAI GTTAAC 1 cut(s) 253
Kzo9I GATC 5 cut(s) 151, 181, 277, 474, 652
LpnPI CCDG 8 cut(s) 27, 198, 398, 509, 626, 653, 678, 695
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 2 cut(s) 417, 685
MaeI CTAG 3 cut(s) 60, 237, 275
MaeIII GTNAC 4 cut(s) 55, 176, 284, 738
MalI GATC 5 cut(s) 153, 183, 279, 476, 654
MboI GATC 5 cut(s) 151, 181, 277, 474, 652
MboII GAAGA 9 cut(s) 202, 263, 350, 518, 541, 544, 625, 734, 826
MfeI CAATTG 1 cut(s) 675
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 69, 76, 357, 372, 675
MlyI GAGTC 1 cut(s) 37
MnlI CCTC 6 cut(s) 142, 289, 328, 628, 672, 726
Mph1103I ATGCAT 1 cut(s) 700
MroI TCCGGA 1 cut(s) 681
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 2 cut(s) 252, 371
MspI CCGG 1 cut(s) 682
MspR9I CCNGG 1 cut(s) 641
MunI CAATTG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 641
MwoI GCNNNNNNNGC 1 cut(s) 374
NcoI CCATGG 1 cut(s) 560
NdeI CATATG 2 cut(s) 463, 700
NdeII GATC 5 cut(s) 151, 181, 277, 474, 652
NlaIII CATG 7 cut(s) 22, 333, 544, 554, 564, 610, 718
NlaIV GGNNCC 2 cut(s) 65, 215
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 3 cut(s) 554, 610, 718
PaeI GCATGC 2 cut(s) 554, 718
PciI ACATGT 1 cut(s) 606
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 616
PkrI GCNGC 1 cut(s) 127
PleI GAGTC 1 cut(s) 37
PpsI GAGTC 1 cut(s) 37
PscI ACATGT 1 cut(s) 606
Psp6I CCWGG 1 cut(s) 639
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 2 cut(s) 65, 215
PspOMI GGGCCC 1 cut(s) 63
PspPI GGNCC 5 cut(s) 63, 64, 214, 493, 637
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 2 cut(s) 91, 749
RsaNI GTAC 2 cut(s) 90, 748
SaqAI TTAA 2 cut(s) 252, 371
SatI GCNGC 1 cut(s) 126
Sau3AI GATC 5 cut(s) 151, 181, 277, 474, 652
Sau96I GGNCC 5 cut(s) 63, 64, 214, 493, 637
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 1 cut(s) 67
SfaNI GCATC 2 cut(s) 417, 685
SinI GGWCC 2 cut(s) 214, 493
SmlI CTYRAG 2 cut(s) 380, 779
SmoI CTYRAG 2 cut(s) 380, 779
SphI GCATGC 2 cut(s) 554, 718
Sse9I AATT 5 cut(s) 69, 76, 357, 372, 675
SsiI CCGC 1 cut(s) 37
SspMI CTAG 3 cut(s) 60, 237, 275
StyD4I CCNGG 1 cut(s) 639
StyI CCWWGG 3 cut(s) 144, 166, 560
TaaI ACNGT 6 cut(s) 89, 94, 139, 290, 308, 824
TaqI TCGA 2 cut(s) 80, 598
TasI AATT 5 cut(s) 69, 76, 357, 372, 675
TatI WGTACW 2 cut(s) 89, 747
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 2 cut(s) 252, 371
Tru9I TTAA 2 cut(s) 252, 371
TscAI CASTG 2 cut(s) 289, 401
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 125
Tsp45I GTSAC 1 cut(s) 284
TspRI CASTG 2 cut(s) 289, 401
VpaK11BI GGWCC 2 cut(s) 214, 493
XapI RAATTY 2 cut(s) 69, 76
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 3 cut(s) 554, 610, 718
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 3 cut(s) 60, 237, 275
ZrmI AGTACT 1 cut(s) 91
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.