Rorug07G0070600

Pentatricopeptide repeat-containing protein At4g18975

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009535
Physical Location & Seq
Forward (+)
5376393 .. 5380685
4293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0070600.1

Sequence Viewer

Length: 2697 bp
ATGGCTCCATTGACACCCATCTCGCTCTCTCTATTAACCCTTCTCGGTCTATTAACATCAGGAGCGCTGTCAACGACATTCACAATGGTAAACAAATGCGAGTACGCAGTGTGGCCGGGCATTCTCTCCAACCCCGGCGTTCCGCAGCTTGCGACCACCGGCTTCGCTCTCCAGAAAGGCGAGACCCAGACCCTAACCGCCCCCGCCTCCTGGAGAGGCCGCTTGTGGGGTCGAACCCTCTGCACCAACGACTCCACCGGAAACTTCTCCTGCGTCACAGGCGATTGCGGCTCCGGAAAGCTAGAATGCTCCGGAAGCGGCGCCGCCCTTCCCGTAACTCTCGCCGAGTTCTCCTTAGGCGGCGCCGGCGGCCTTGACTTCTTTGACGTCAGCCTCGTCGACGGTTACAACCTCCCTATGCTGGTGGCTCCCCAGGGTGGGACCGGCACCAATTGTACCAACACCGGCTGCGTGGTCGACCTTAACGGCGTTTGTCCCTCGGAGCTCCAGGTGACTAGCGCTGCGGGAAGCGAAGTCGTCGGGTGCAGGAGCGCGTGCGAGGTGGTCCAGCGGCCGCTGTACTGTTGCATCGAGGCGTACGCTTCACCCGACACGTGCAAGCCTTCTTTGTACTCGCAAATGTTCAAGAACGCGTGCCCTCTCGCGTACAGTTACGCATACGACGATAAGACCAGCACCTTCACATGTGCCAATGCCAATTACACCGTCACCTTCTGTCCCTCTCCGAACACCAGGTTACCATCTCTTCAACCCGAAGACGATCCCCAATACACACAGTGCAAGCAACCTTTCAATTGTGGTAACCTCACAGGCATATCCTACCCTTTCTGGGGAGGACCCAATCGCCCCCAAGAGTGCGGTCGCTTAGGCTTCGAACTCACCAACTGCGAAGACGAAACTCAACTCCCTCACATAGTAATCGAAAAGCTAGATTTCCATGTGTCCAACATCAACAGCCAAGATCTTCTTCACACCATGACAATCGCTCGCTCCGACCTCTGGGACAGTCCTTGTACTGACCTCCTCGTCAACACCACTCTCGACTACAACCGTTTCTCTTATGTCCAAACTGTTCGCAACCTCACCTTGTACTACGGCTGCCTCCCTCAAAACGAATCGGTCCTCAACAATTTTACGTGCAGAATAGACGGCACAGATAAGGATAGAAATATTTCGTACTACGTGGATGATTCACTCTCAATGGTTAATGTTTCGGGCCGACCCACATGTTCTACCAATATTCGAGTCCCCATTTTTTGGGAGGGCTTTGATGTTATGCCAGACAATGCAACGGAAGAAGTGGGAAAGGTTTTGAAGCAAGGGTTTCAGGTTGAGTACAGCGCGGATTGGGATCTCTGTAGGTCATGCATGAACTCTAATGGCACCTGTGGATCGAACGCAACGGCGGATTCGTTTCTCTGCCTTTGCGGGGATCGTCCTTCCAATTCTACTTGTCCAATTGTTCATGGCACCAAGTTCAACTGGAAAACAAAGGTTTTCATAGGCGTAGCTGCGACTATTGCAGGATCTTGTATTTTATTGTTTGTTATTCTCTTCCGTTGGACTTCACTGATTAGGGGAAATGACAATGAAGATGCTGAGGCCTTCATTCAGAACATTGGACCTCTAGCAGTAAAAAGATACAAATTTTCGGATGTAAGGAAAATGACCAACTCATTTAAAGATAAACTGGGTCAAGGCGGTTATGGTGATGTGTACGAAGGGAAGCTATTGAATGGTTGTCGTGTGGCTGTGAAGGTTCTTAAAGAATCCAAAGGGAATGGAGAGGACTTTGTTAATGAGGTTGCTAGCATTAGTAGAACTTCCCATGTTAATGTGGTCACTCTTTTGGGGTATTGCTTTGAAGGTAAGAAAAAAGCGCTCATATATGAGTTCATGCCCAATGGATCACTTGAGAAGTTCATATACAAAGACAGTAATCTTTTGACAACCACTCCACACTTGGAATTGGAAAAACTCTTCCAAATTGCTATCGGGATAGCTCGAGGGCTTGAGTACTTGCACCGTGGGTGCAACACACGAATCCTGCACTTTGACATAAAACCACATAATATCCTTTTGGATGAAAACTTTTTCCCAAAGATTTCTGACTTTGGTCTTTCTAAACTTTGTACAAGGAAGGAGAGCATTATTTCAATGTTGGATGCTAGAGGGACAATAGGGTATATGGCTCCAGAAGTGTTCTCCAGAAACTTTGGAAGAGTGTCTGCTAAGTCCGATGTCTATAGTTATGGAATGATGATTCTGGAGATGGTTGGAGGAAGAAAGAATGTTGAAGCTCGAGTAAGCCGCACCAGTGAAATTTATTTTCCAGATTGGGTTTATGAGCATCTTGAGCAGGGCAGCAATTTTGGATTGCTCAGTGCCGCGACTGAAGAGGAAAAGGAACTTGCGAGGAAGATGATCTTAGTGGGAATGTGGTGCATACAGACAAAGCCATCAGATAGGCCATCCATGAGTAAAGTGATTGAAATGTTGGAAGGAAGCACTGAAGCCTTGCAAATACCACCAAAGCCTGTCCTATCTTCTCCTGTACAGCAATTCACCACAAACAAATCTGAAAGCATTGTTTTGCTACTGTACATAAACATACAGAAGCAGTGTAATGTACAGGATCTTATCAGAACCTCCAACACTGTAGACTGCATTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

898

Amino Acids

98.87

Weight (kDa)

5.43

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thaumatin PF00314 31 - 247 4.7e-81 Thaumatin family
GUB_WAK_bind PF13947 267 - 335 3.9e-11 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 387 - 485 3.4e-15 Wall-associated receptor kinase C-terminal
PK_Tyr_Ser-Thr PF07714 567 - 840 3.4e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 568 - 838 1.7e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009535)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04590 AT1G04590
fragaria_vesca FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240 FvH4_5g00240
malus_domestica MD06G1063200.v1.1
prunus_persica Prupe.5G075000_v2.0.a1
pyrus_communis pycom06g06020
rosa_chinensis RchiOBHm_Chr7g0202441
rosa_laevigata RLG00000003603
rosa_multiflora Rmu_sc0031703.1_g000002
rosa_roxburghii Rroxscaffold_3G00254610
rosa_rugosa Rorug07G0070600
rosa_samantha Rh7AG198000 Rh7BG199300 Rh7CG209100 Rh7DG204900
rosa_wichuraiana Rw7G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1046
AatII GACGTC 1 cut(s) 390
AccB1I GGYRCC 5 cut(s) 320, 362, 446, 1403, 1490
AccB7I CCANNNNNTGG 1 cut(s) 1278
AccI GTMKAC 3 cut(s) 399, 477, 2682
AccII CGCG 5 cut(s) 554, 653, 665, 1364, 2411
AccIII TCCGGA 2 cut(s) 293, 311
AclWI GGATC 7 cut(s) 776, 1380, 1420, 1461, 1555, 1936, 2664
AcoI YGGCCR 2 cut(s) 113, 572
AcsI RAATTY 2 cut(s) 1667, 2343
AcuI CTGAAG 2 cut(s) 2436, 2553
AcvI CACGTG 1 cut(s) 615
AcyI GRCGYC 3 cut(s) 321, 363, 387
AdeI CACNNNGTG 1 cut(s) 798
AfeI AGCGCT 3 cut(s) 66, 520, 1902
AflIII ACRYGT 4 cut(s) 612, 651, 704, 1247
AjnI CCWGG 4 cut(s) 209, 432, 507, 752
AjuI GAANNNNNNNTTGG 2 cut(s) 972, 1004
AluBI AGCT 8 cut(s) 148, 301, 505, 949, 1532, 1750, 2024, 2321
AluI AGCT 8 cut(s) 148, 301, 505, 949, 1532, 1750, 2024, 2321
Alw21I GWGCWC 1 cut(s) 507
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 7 cut(s) 776, 1380, 1420, 1461, 1555, 1936, 2664
Ama87I CYCGRG 2 cut(s) 2025, 2322
Aor13HI TCCGGA 2 cut(s) 293, 311
Aor51HI AGCGCT 3 cut(s) 66, 520, 1902
AoxI GGCC 7 cut(s) 113, 217, 370, 572, 1237, 1623, 2489
ApeKI GCWGC 6 cut(s) 145, 468, 521, 1119, 1532, 2385
ApoI RAATTY 2 cut(s) 1667, 2343
Asp700I GAANNNNTTC 1 cut(s) 1192
AspLEI GCGC 7 cut(s) 67, 323, 365, 521, 554, 1364, 1903
AspS9I GGNCC 6 cut(s) 441, 565, 857, 1141, 1237, 1643
AsuC2I CCSGG 2 cut(s) 117, 135
AsuHPI GGTGA 7 cut(s) 523, 597, 721, 892, 1096, 1742, 2578
AsuII TTCGAA 1 cut(s) 894
AsuNHI GCTAGC 1 cut(s) 1829
AvaI CYCGRG 2 cut(s) 2025, 2322
AvaII GGWCC 5 cut(s) 441, 565, 857, 1141, 1643
AxyI CCTNAGG 1 cut(s) 355
BaeGI GKGCMC 1 cut(s) 659
BanI GGYRCC 5 cut(s) 320, 362, 446, 1403, 1490
BanII GRGCYC 1 cut(s) 507
BarI GAAGNNNNNNTAC 2 cut(s) 1931, 1963
BbrPI CACGTG 1 cut(s) 615
BbsI GAAGAC 2 cut(s) 783, 918
Bbv12I GWGCWC 1 cut(s) 507
BbvCI CCTCAGC 1 cut(s) 1620
BbvI GCAGC 6 cut(s) 157, 455, 508, 1106, 1519, 2397
BccI CCATC 5 cut(s) 26, 769, 2287, 2488, 2500
BceAI ACGGC 4 cut(s) 502, 1132, 1186, 1440
BcgI CGANNNNNNTGC 2 cut(s) 222, 256
BciT130I CCWGG 4 cut(s) 211, 434, 509, 754
BcnI CCSGG 2 cut(s) 117, 135
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 6 cut(s) 302, 516, 950, 1649, 1830, 2190
BfmI CTRYAG 3 cut(s) 1378, 2266, 2679
BfoI RGCGCY 5 cut(s) 68, 324, 366, 522, 1904
BglII AGATCT 1 cut(s) 982
BmcAI AGTACT 1 cut(s) 2039
Bme1390I CCNGG 6 cut(s) 117, 135, 211, 434, 509, 754
Bme18I GGWCC 5 cut(s) 441, 565, 857, 1141, 1643
BmeT110I CYCGRG 2 cut(s) 2025, 2322
BmgT120I GGNCC 6 cut(s) 441, 565, 857, 1141, 1237, 1643
BmrFI CCNGG 6 cut(s) 117, 135, 211, 434, 509, 754
BmrI ACTGGG 1 cut(s) 1721
BmsI GCATC 4 cut(s) 597, 1606, 2176, 2380
BmtI GCTAGC 1 cut(s) 1833
BmuI ACTGGG 1 cut(s) 1721
BoxI GACNNNNGTC 1 cut(s) 2136
BpiI GAAGAC 2 cut(s) 783, 918
BpmI CTGGAG 6 cut(s) 155, 232, 491, 2199, 2212, 2309
Bpu10I CCTNAGC 2 cut(s) 886, 1620
Bpu14I TTCGAA 1 cut(s) 894
BpuEI CTTGAG 3 cut(s) 1955, 2054, 2396
BpuMI CCSGG 2 cut(s) 117, 135
BsaAI YACGTR 3 cut(s) 615, 1158, 1204
BsaBI GATNNNNATC 1 cut(s) 1371
BsaHI GRCGYC 3 cut(s) 321, 363, 387
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 5 cut(s) 133, 432, 433, 498, 2047
BsaWI WCCGGW 3 cut(s) 257, 293, 311
BsaXI ACNNNNNCTCC 2 cut(s) 1960, 1990
Bse118I RCCGGY 4 cut(s) 158, 365, 443, 464
Bse1I ACTGG 3 cut(s) 1508, 1716, 2337
Bse21I CCTNAGG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 1371
BseAI TCCGGA 2 cut(s) 293, 311
BseBI CCWGG 4 cut(s) 211, 434, 509, 754
BseDI CCNNGG 5 cut(s) 133, 432, 433, 498, 2047
BseGI GGATG 5 cut(s) 1213, 1681, 2110, 2191, 2492
BseJI GATNNNNATC 1 cut(s) 1371
BseMII CTCAG 2 cut(s) 1611, 2416
BseNI ACTGG 3 cut(s) 1508, 1716, 2337
BseRI GAGGAG 1 cut(s) 1034
BseSI GKGCMC 1 cut(s) 659
BseX3I CGGCCG 1 cut(s) 572
BseXI GCAGC 6 cut(s) 157, 455, 508, 1106, 1519, 2397
BsgI GTGCAG 4 cut(s) 226, 565, 1180, 2054
Bsh1236I CGCG 5 cut(s) 554, 653, 665, 1364, 2411
Bsh1285I CGRYCG 2 cut(s) 575, 883
BshFI GGCC 7 cut(s) 115, 219, 372, 574, 1239, 1625, 2491
BshNI GGYRCC 5 cut(s) 320, 362, 446, 1403, 1490
BsiEI CGRYCG 2 cut(s) 575, 883
BsiHKAI GWGCWC 1 cut(s) 507
BsiHKCI CYCGRG 2 cut(s) 2025, 2322
BsiSI CCGG 9 cut(s) 116, 135, 159, 258, 294, 312, 366, 444, 465
BsiWI CGTACG 1 cut(s) 597
BslFI GGGAC 6 cut(s) 454, 480, 723, 1037, 1253, 2209
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 6 cut(s) 454, 480, 723, 1037, 1253, 2209
BsmI GAATGC 2 cut(s) 120, 311
BsnI GGCC 7 cut(s) 115, 219, 372, 574, 1239, 1625, 2491
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 2 cut(s) 2025, 2322
Bsp119I TTCGAA 1 cut(s) 894
Bsp1286I GDGCHC 2 cut(s) 507, 659
Bsp13I TCCGGA 2 cut(s) 293, 311
Bsp1407I TGTACA 4 cut(s) 2153, 2575, 2622, 2650
Bsp143I GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
BspANI GGCC 7 cut(s) 115, 219, 372, 574, 1239, 1625, 2491
BspCNI CTCAG 2 cut(s) 1612, 2415
BspEI TCCGGA 2 cut(s) 293, 311
BspFNI CGCG 5 cut(s) 554, 653, 665, 1364, 2411
BspOI GCTAGC 1 cut(s) 1833
BspPI GGATC 7 cut(s) 776, 1380, 1420, 1461, 1555, 1936, 2664
BspT104I TTCGAA 1 cut(s) 894
BspT107I GGYRCC 5 cut(s) 320, 362, 446, 1403, 1490
BspTNI GGTCTC 1 cut(s) 176
BsrFI RCCGGY 4 cut(s) 158, 365, 443, 464
BsrGI TGTACA 4 cut(s) 2153, 2575, 2622, 2650
BsrI ACTGG 3 cut(s) 1508, 1716, 2337
BssAI RCCGGY 4 cut(s) 158, 365, 443, 464
BssECI CCNNGG 5 cut(s) 133, 432, 433, 498, 2047
BssMI GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
BssNI GRCGYC 3 cut(s) 321, 363, 387
Bst2UI CCWGG 4 cut(s) 211, 434, 509, 754
Bst6I CTCTTC 5 cut(s) 771, 1580, 2006, 2236, 2412
BstACI GRCGYC 3 cut(s) 321, 363, 387
BstAUI TGTACA 4 cut(s) 2153, 2575, 2622, 2650
BstBAI YACGTR 3 cut(s) 615, 1158, 1204
BstBI TTCGAA 1 cut(s) 894
BstC8I GCNNGC 8 cut(s) 150, 367, 556, 620, 655, 803, 1009, 1831
BstDEI CTNAG 6 cut(s) 355, 886, 1620, 2253, 2402, 2449
BstDSI CCRYGG 1 cut(s) 2047
BstEII GGTNACC 2 cut(s) 756, 821
BstF5I GGATG 5 cut(s) 1213, 1681, 2110, 2191, 2492
BstFNI CGCG 5 cut(s) 554, 653, 665, 1364, 2411
BstH2I RGCGCY 5 cut(s) 68, 324, 366, 522, 1904
BstHHI GCGC 7 cut(s) 67, 323, 365, 521, 554, 1364, 1903
BstKTI GATC 9 cut(s) 784, 985, 1375, 1415, 1456, 1550, 1931, 2448, 2659
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
BstMCI CGRYCG 2 cut(s) 575, 883
BstMWI GCNNNNNNNGC 7 cut(s) 279, 288, 315, 366, 369, 1541, 2377
BstNI CCWGG 4 cut(s) 211, 434, 509, 754
BstNSI RCATGY 2 cut(s) 708, 1251
BstPAI GACNNNNGTC 1 cut(s) 2136
BstPI GGTNACC 2 cut(s) 756, 821
BstSCI CCNGG 6 cut(s) 115, 133, 209, 432, 507, 752
BstSFI CTRYAG 3 cut(s) 1378, 2266, 2679
BstSLI GKGCMC 1 cut(s) 659
BstUI CGCG 5 cut(s) 554, 653, 665, 1364, 2411
BstV1I GCAGC 6 cut(s) 157, 455, 508, 1106, 1519, 2397
BstV2I GAAGAC 2 cut(s) 783, 918
BstX2I RGATCY 4 cut(s) 982, 1372, 1547, 2656
BstYI RGATCY 4 cut(s) 982, 1372, 1547, 2656
BstZI CGGCCG 1 cut(s) 572
Bsu36I CCTNAGG 1 cut(s) 355
BsuRI GGCC 7 cut(s) 115, 219, 372, 574, 1239, 1625, 2491
BtgI CCRYGG 1 cut(s) 2047
BtsCI GGATG 5 cut(s) 1213, 1681, 2110, 2191, 2492
BtsI GCAGTG 2 cut(s) 114, 2648
BtsIMutI CAGTG 8 cut(s) 114, 803, 1589, 2344, 2410, 2529, 2648, 2676
Cac8I GCNNGC 8 cut(s) 150, 367, 556, 620, 655, 803, 1009, 1831
CciNI GCGGCCGC 1 cut(s) 572
CfoI GCGC 7 cut(s) 67, 323, 365, 521, 554, 1364, 1903
Cfr10I RCCGGY 4 cut(s) 158, 365, 443, 464
Cfr13I GGNCC 6 cut(s) 441, 565, 857, 1141, 1237, 1643
CseI GACGC 1 cut(s) 262
CsiI ACCWGGT 1 cut(s) 752
DdeI CTNAG 6 cut(s) 355, 886, 1620, 2253, 2402, 2449
DinI GGCGCC 2 cut(s) 322, 364
DpnI GATC 9 cut(s) 783, 984, 1374, 1414, 1455, 1549, 1930, 2447, 2658
DpnII GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
DraI TTTAAA 1 cut(s) 1702
DraIII CACNNNGTG 1 cut(s) 798
DrdI GACNNNNNNGTC 1 cut(s) 1046
DseDI GACNNNNNNGTC 1 cut(s) 1046
EaeI YGGCCR 2 cut(s) 113, 572
EagI CGGCCG 1 cut(s) 572
Eam1104I CTCTTC 5 cut(s) 771, 1580, 2006, 2236, 2412
EarI CTCTTC 5 cut(s) 771, 1580, 2006, 2236, 2412
EciI GGCGGA 1 cut(s) 1442
Ecl136II GAGCTC 1 cut(s) 505
EclXI CGGCCG 1 cut(s) 572
Eco147I AGGCCT 1 cut(s) 1625
Eco24I GRGCYC 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 176
Eco47I GGWCC 5 cut(s) 441, 565, 857, 1141, 1643
Eco47III AGCGCT 3 cut(s) 66, 520, 1902
Eco52I CGGCCG 1 cut(s) 572
Eco53kI GAGCTC 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 2436, 2553
Eco72I CACGTG 1 cut(s) 615
Eco81I CCTNAGG 1 cut(s) 355
Eco88I CYCGRG 2 cut(s) 2025, 2322
Eco91I GGTNACC 2 cut(s) 756, 821
EcoICRI GAGCTC 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 857
EcoO65I GGTNACC 2 cut(s) 756, 821
EcoRII CCWGG 4 cut(s) 209, 432, 507, 752
EcoT22I ATGCAT 1 cut(s) 1391
EcoT38I GRGCYC 1 cut(s) 507
EgeI GGCGCC 2 cut(s) 322, 364
EheI GGCGCC 2 cut(s) 322, 364
FalI AAGNNNNNCTT 4 cut(s) 972, 1004, 2432, 2464
FaqI GGGAC 6 cut(s) 454, 480, 723, 1037, 1253, 2209
FauI CCCGC 3 cut(s) 211, 517, 1442
FblI GTMKAC 3 cut(s) 399, 477, 2682
FokI GGATG 5 cut(s) 1220, 1688, 2117, 2198, 2479
FriOI GRGCYC 1 cut(s) 507
FspBI CTAG 6 cut(s) 302, 516, 950, 1649, 1830, 2190
GlaI GCGC 7 cut(s) 66, 322, 364, 520, 553, 1363, 1902
GsuI CTGGAG 6 cut(s) 155, 232, 491, 2199, 2212, 2309
HaeII RGCGCY 5 cut(s) 68, 324, 366, 522, 1904
HaeIII GGCC 7 cut(s) 115, 219, 372, 574, 1239, 1625, 2491
HapII CCGG 9 cut(s) 116, 135, 159, 258, 294, 312, 366, 444, 465
HgaI GACGC 1 cut(s) 262
HhaI GCGC 7 cut(s) 67, 323, 365, 521, 554, 1364, 1903
Hin1I GRCGYC 3 cut(s) 321, 363, 387
Hin6I GCGC 7 cut(s) 65, 321, 363, 519, 552, 1362, 1901
HinP1I GCGC 7 cut(s) 65, 321, 363, 519, 552, 1362, 1901
HincII GTYRAC 4 cut(s) 72, 400, 478, 1051
HindII GTYRAC 4 cut(s) 72, 400, 478, 1051
HinfI GANTC 8 cut(s) 251, 1136, 1211, 1266, 1430, 1790, 2064, 2284
HpaII CCGG 9 cut(s) 116, 135, 159, 258, 294, 312, 366, 444, 465
HphI GGTGA 7 cut(s) 523, 597, 721, 892, 1096, 1742, 2578
Hpy166II GTNNAC 7 cut(s) 72, 91, 400, 478, 1051, 1738, 2683
Hpy8I GTNNAC 7 cut(s) 72, 91, 400, 478, 1051, 1738, 2683
Hpy99I CGWCG 4 cut(s) 401, 404, 542, 686
HpyCH4IV ACGT 4 cut(s) 387, 614, 1157, 1203
HpyF10VI GCNNNNNNNGC 7 cut(s) 279, 288, 315, 366, 369, 1541, 2377
HpyF3I CTNAG 6 cut(s) 355, 886, 1620, 2253, 2402, 2449
HpySE526I ACGT 4 cut(s) 387, 614, 1157, 1203
Hsp92I GRCGYC 3 cut(s) 321, 363, 387
HspAI GCGC 7 cut(s) 65, 321, 363, 519, 552, 1362, 1901
KasI GGCGCC 2 cut(s) 320, 362
Kpn2I TCCGGA 2 cut(s) 293, 311
KroI GCCGGC 1 cut(s) 365
KroNI GCCGGC 1 cut(s) 367
Kzo9I GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
Lsp1109I GCAGC 6 cut(s) 157, 455, 508, 1106, 1519, 2397
LweI GCATC 4 cut(s) 597, 1606, 2176, 2380
MabI ACCWGGT 1 cut(s) 752
MaeI CTAG 6 cut(s) 302, 516, 950, 1649, 1830, 2190
MaeII ACGT 4 cut(s) 387, 614, 1157, 1203
MaeIII GTNAC 9 cut(s) 274, 334, 404, 511, 671, 727, 756, 821, 1861
MalI GATC 9 cut(s) 783, 984, 1374, 1414, 1455, 1549, 1930, 2447, 2658
MboI GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
MfeI CAATTG 3 cut(s) 451, 814, 1479
MflI RGATCY 4 cut(s) 982, 1372, 1547, 2656
MhlI GDGCHC 2 cut(s) 507, 659
MluI ACGCGT 1 cut(s) 651
Mly113I GGCGCC 2 cut(s) 321, 363
MlyI GAGTC 2 cut(s) 245, 1275
MmeI TCCRAC 8 cut(s) 153, 990, 1038, 1562, 2163, 2278, 2499, 2697
Mph1103I ATGCAT 1 cut(s) 1391
MreI CGCCGGCG 1 cut(s) 365
MroI TCCGGA 2 cut(s) 293, 311
MroNI GCCGGC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 1192
MseI TTAA 9 cut(s) 35, 53, 483, 1227, 1701, 1785, 1818, 1854, 2695
MslI CAYNNNNRTG 1 cut(s) 1854
MspA1I CMGCKG 2 cut(s) 571, 577
MspI CCGG 9 cut(s) 116, 135, 159, 258, 294, 312, 366, 444, 465
MspR9I CCNGG 6 cut(s) 117, 135, 211, 434, 509, 754
MunI CAATTG 3 cut(s) 451, 814, 1479
Mva1269I GAATGC 2 cut(s) 120, 311
MvaI CCWGG 4 cut(s) 211, 434, 509, 754
MvnI CGCG 5 cut(s) 554, 653, 665, 1364, 2411
MwoI GCNNNNNNNGC 7 cut(s) 279, 288, 315, 366, 369, 1541, 2377
NaeI GCCGGC 1 cut(s) 367
NarI GGCGCC 2 cut(s) 321, 363
NciI CCSGG 2 cut(s) 117, 135
NdeII GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
NgoMIV GCCGGC 1 cut(s) 365
NheI GCTAGC 1 cut(s) 1829
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 4 cut(s) 274, 511, 727, 1861
NotI GCGGCCGC 1 cut(s) 572
NsiI ATGCAT 1 cut(s) 1391
NspI RCATGY 2 cut(s) 708, 1251
NspV TTCGAA 1 cut(s) 894
PaeR7I CTCGAG 2 cut(s) 2025, 2322
PasI CCCWGGG 1 cut(s) 433
PceI AGGCCT 1 cut(s) 1625
PciI ACATGT 2 cut(s) 704, 1247
PcsI WCGNNNNNNNCGW 5 cut(s) 393, 606, 681, 1011, 1430
PctI GAATGC 2 cut(s) 120, 311
PdiI GCCGGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 1192
PfeI GAWTC 6 cut(s) 1136, 1211, 1430, 1790, 2064, 2284
Pfl23II CGTACG 1 cut(s) 597
PflMI CCANNNNNTGG 1 cut(s) 1278
PfoI TCCNGGA 1 cut(s) 209
PleI GAGTC 2 cut(s) 245, 1274
PluTI GGCGCC 2 cut(s) 324, 366
PmaCI CACGTG 1 cut(s) 615
PmlI CACGTG 1 cut(s) 615
PpsI GAGTC 2 cut(s) 245, 1274
Ppu21I YACGTR 3 cut(s) 615, 1158, 1204
PpuMI RGGWCCY 1 cut(s) 857
PscI ACATGT 2 cut(s) 704, 1247
PshAI GACNNNNGTC 1 cut(s) 2136
Psp124BI GAGCTC 1 cut(s) 507
Psp5II RGGWCCY 1 cut(s) 857
Psp6I CCWGG 4 cut(s) 209, 432, 507, 752
PspCI CACGTG 1 cut(s) 615
PspEI GGTNACC 2 cut(s) 756, 821
PspGI CCWGG 4 cut(s) 209, 432, 507, 752
PspLI CGTACG 1 cut(s) 597
PspPI GGNCC 6 cut(s) 441, 565, 857, 1141, 1237, 1643
PspPPI RGGWCCY 1 cut(s) 857
PspXI VCTCGAGB 2 cut(s) 2025, 2322
PsuI RGATCY 4 cut(s) 982, 1372, 1547, 2656
RseI CAYNNNNRTG 1 cut(s) 1854
SacI GAGCTC 1 cut(s) 507
SalI GTCGAC 2 cut(s) 398, 476
SaqAI TTAA 9 cut(s) 35, 53, 483, 1227, 1701, 1785, 1818, 1854, 2695
Sau3AI GATC 9 cut(s) 781, 982, 1372, 1412, 1453, 1547, 1928, 2445, 2656
Sau96I GGNCC 6 cut(s) 441, 565, 857, 1141, 1237, 1643
ScaI AGTACT 1 cut(s) 2039
SchI GAGTC 2 cut(s) 245, 1275
ScrFI CCNGG 6 cut(s) 117, 135, 211, 434, 509, 754
SduI GDGCHC 2 cut(s) 507, 659
SexAI ACCWGGT 1 cut(s) 752
SfaNI GCATC 4 cut(s) 597, 1606, 2176, 2380
SfcI CTRYAG 3 cut(s) 1378, 2266, 2679
SfoI GGCGCC 2 cut(s) 322, 364
Sfr274I CTCGAG 2 cut(s) 2025, 2322
SfuI TTCGAA 1 cut(s) 894
SgrAI CRCCGGYG 1 cut(s) 365
SgrDI CGTCGACG 1 cut(s) 398
SinI GGWCC 5 cut(s) 441, 565, 857, 1141, 1643
SlaI CTCGAG 2 cut(s) 2025, 2322
SmiMI CAYNNNNRTG 1 cut(s) 1854
SmlI CTYRAG 5 cut(s) 1934, 2025, 2033, 2322, 2375
SmoI CTYRAG 5 cut(s) 1934, 2025, 2033, 2322, 2375
SseBI AGGCCT 1 cut(s) 1625
SspDI GGCGCC 2 cut(s) 320, 362
SspI AATATT 2 cut(s) 1192, 1261
SspMI CTAG 6 cut(s) 302, 516, 950, 1649, 1830, 2190
SstI GAGCTC 1 cut(s) 507
StuI AGGCCT 1 cut(s) 1625
StyD4I CCNGG 6 cut(s) 115, 133, 209, 432, 507, 752
TaiI ACGT 4 cut(s) 390, 617, 1160, 1206
TaqII GACCGA 2 cut(s) 35, 1129
TfiI GAWTC 6 cut(s) 1136, 1211, 1430, 1790, 2064, 2284
Tru1I TTAA 9 cut(s) 35, 53, 483, 1227, 1701, 1785, 1818, 1854, 2695
Tru9I TTAA 9 cut(s) 35, 53, 483, 1227, 1701, 1785, 1818, 1854, 2695
TscAI CASTG 8 cut(s) 114, 803, 1596, 2344, 2410, 2536, 2648, 2683
TseFI GTSAC 4 cut(s) 274, 511, 727, 1861
TseI GCWGC 6 cut(s) 145, 468, 521, 1119, 1532, 2385
Tsp45I GTSAC 4 cut(s) 274, 511, 727, 1861
TspDTI ATGAA 8 cut(s) 1406, 1475, 1510, 1618, 1626, 1906, 1933, 2121
TspGWI ACGGA 2 cut(s) 1328, 1568
TspRI CASTG 8 cut(s) 114, 803, 1596, 2344, 2410, 2536, 2648, 2683
Van91I CCANNNNNTGG 1 cut(s) 1278
VpaK11BI GGWCC 5 cut(s) 441, 565, 857, 1141, 1643
XapI RAATTY 2 cut(s) 1667, 2343
XceI RCATGY 2 cut(s) 708, 1251
XcmI CCANNNNNNNNNTGG 1 cut(s) 1981
XhoI CTCGAG 2 cut(s) 2025, 2322
XmiI GTMKAC 3 cut(s) 399, 477, 2682
XmnI GAANNNNTTC 1 cut(s) 1192
XspI CTAG 6 cut(s) 302, 516, 950, 1649, 1830, 2190
ZraI GACGTC 1 cut(s) 388
ZrmI AGTACT 1 cut(s) 2039
Zsp2I ATGCAT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.