pycom06g10010

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0019819
Physical Location & Seq
Forward (+)
15352890 .. 15355232
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10010.3

Sequence Viewer

Length: 627 bp
ATGGAGAAATTTGTGAGGGTAGTTTCCATGGCTCCTCTGCTTCTGCTGCTCTGTTTTCCTTTTGCTTTGGCCGGCCATGACTACGGGCAGGCTCTGAGCAACAGCATTCTGTTCTTTGTAGCTCAGAGATCTGGGTTTCTTCCCCCCAACCAGAGAGTCACTTGGAGATCCAATTCTGGCTTGTATGATGGCAAGGCCAATGGGGTGGATCTGGTGGGAGGGTACTATGATGCAGGTGACAATGTGAAGTTTGGGCTTCCCATGGCCTTCACAGTGACAATGATGTCCTGGAGTATAATCGAGTACGGCAGCCAAATGGCTGCAAGTGGCGAGCTCGGCCATGCCATGGATGCCGTCAAATGGGGCACTGACTACTTCATCAAAGCCCACCCTGAACCCAATGTCCTCTATGGCGAGGTGGGAGATGGGAACACTGACCATTACTGTTGGCAAAGGCCGGAGGACATGACCACTAACCGCCAGGCTTACAAGATCGAGCCCCAGCAATCCCGGGATCGATCTTGCCGGGGAAACCGCCGCCGCCATGGCCGCTGCCTCCATTGTCTTCCGCCGCTCCAACCCCGCATACTCCCGCGAGCTCCTCAGCCACGCGTATCAGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

209

Amino Acids

23.31

Weight (kDa)

8.84

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019819)

Species Orthologous Gene IDs
pyrus_communis pycom06g10010 pycom17g12680
rosa_chinensis RchiOBHm_Chr0c20g0500181 RchiOBHm_Chr1g0340171
rosa_multiflora Rmu_sc0003311.1_g000003 Rmu_sc0032132.1_g000001
rosa_samantha Rh1CG166700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 574
AccII CGCG 2 cut(s) 595, 612
AciI CCGC 9 cut(s) 478, 535, 538, 541, 550, 569, 572, 583, 593
AclWI GGATC 3 cut(s) 162, 216, 522
AcoI YGGCCR 4 cut(s) 69, 73, 337, 547
AcsI RAATTY 1 cut(s) 8
AfaI GTAC 2 cut(s) 224, 305
AfiI CCNNNNNNNGG 2 cut(s) 346, 360
AflIII ACRYGT 1 cut(s) 610
AjnI CCWGG 2 cut(s) 287, 480
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 4 cut(s) 122, 334, 599, 620
AluI AGCT 4 cut(s) 122, 334, 599, 620
Alw21I GWGCWC 2 cut(s) 336, 601
AlwI GGATC 3 cut(s) 162, 216, 522
AlwNI CAGNNNCTG 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 7 cut(s) 69, 73, 195, 264, 337, 455, 547
ApeKI GCWGC 4 cut(s) 46, 309, 320, 552
ApoI RAATTY 1 cut(s) 8
AsuC2I CCSGG 3 cut(s) 511, 512, 527
AsuHPI GGTGA 1 cut(s) 248
AvaI CYCGRG 1 cut(s) 510
BaeGI GKGCMC 1 cut(s) 368
BanII GRGCYC 3 cut(s) 336, 501, 601
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 2 cut(s) 336, 601
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 4 cut(s) 33, 307, 321, 539
BccI CCATC 2 cut(s) 182, 419
BceAI ACGGC 2 cut(s) 322, 338
BciT130I CCWGG 2 cut(s) 289, 482
BcnI CCSGG 3 cut(s) 511, 512, 527
BfuAI ACCTGC 1 cut(s) 224
BglI GCCNNNNNGGC 1 cut(s) 546
BglII AGATCT 1 cut(s) 128
BisI GCNGC 8 cut(s) 47, 310, 321, 538, 541, 550, 553, 572
BlsI GCNGC 8 cut(s) 48, 311, 322, 539, 542, 551, 554, 573
Bme1390I CCNGG 5 cut(s) 289, 482, 511, 512, 527
BmeT110I CYCGRG 1 cut(s) 510
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 5 cut(s) 289, 482, 511, 512, 527
BmsI GCATC 2 cut(s) 220, 340
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 603
BpuMI CCSGG 3 cut(s) 511, 512, 527
Bsa29I ATCGAT 1 cut(s) 517
BsaJI CCNNGG 6 cut(s) 27, 261, 345, 510, 526, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 360
Bse118I RCCGGY 1 cut(s) 71
BseBI CCWGG 2 cut(s) 289, 482
BseCI ATCGAT 1 cut(s) 517
BseDI CCNNGG 6 cut(s) 27, 261, 345, 510, 526, 544
BseGI GGATG 1 cut(s) 355
BseLI CCNNNNNNNGG 2 cut(s) 346, 360
BseMII CTCAG 3 cut(s) 86, 137, 617
BseRI GAGGAG 2 cut(s) 24, 591
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 4 cut(s) 33, 307, 321, 539
BseYI CCCAGC 1 cut(s) 501
Bsh1236I CGCG 2 cut(s) 595, 612
BshFI GGCC 7 cut(s) 71, 75, 197, 266, 339, 457, 549
BshVI ATCGAT 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 336, 601
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 4 cut(s) 72, 458, 511, 526
BslI CCNNNNNNNGG 2 cut(s) 346, 360
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 7 cut(s) 71, 75, 197, 266, 339, 457, 549
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 4 cut(s) 336, 368, 501, 601
Bsp143I GATC 6 cut(s) 128, 167, 208, 492, 514, 518
Bsp19I CCATGG 4 cut(s) 27, 261, 345, 544
BspACI CCGC 9 cut(s) 478, 535, 538, 541, 550, 569, 572, 583, 593
BspANI GGCC 7 cut(s) 71, 75, 197, 266, 339, 457, 549
BspCNI CTCAG 3 cut(s) 87, 136, 616
BspDI ATCGAT 1 cut(s) 517
BspFNI CGCG 2 cut(s) 595, 612
BspLI GGNNCC 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 3 cut(s) 162, 216, 522
BsrBI CCGCTC 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 6 cut(s) 27, 261, 345, 510, 526, 544
BssMI GATC 6 cut(s) 128, 167, 208, 492, 514, 518
BssT1I CCWWGG 4 cut(s) 27, 261, 345, 544
Bst2UI CCWGG 2 cut(s) 289, 482
Bst4CI ACNGT 2 cut(s) 274, 446
BstC8I GCNNGC 4 cut(s) 73, 90, 332, 597
BstDEI CTNAG 3 cut(s) 95, 123, 603
BstDSI CCRYGG 4 cut(s) 27, 261, 345, 544
BstF5I GGATG 1 cut(s) 355
BstFNI CGCG 2 cut(s) 595, 612
BstKTI GATC 6 cut(s) 131, 170, 211, 495, 517, 521
BstMBI GATC 6 cut(s) 128, 167, 208, 492, 514, 518
BstMWI GCNNNNNNNGC 5 cut(s) 46, 336, 350, 546, 549
BstNI CCWGG 2 cut(s) 289, 482
BstSCI CCNGG 5 cut(s) 287, 480, 509, 510, 525
BstSLI GKGCMC 1 cut(s) 368
BstUI CGCG 2 cut(s) 595, 612
BstV1I GCAGC 4 cut(s) 33, 307, 321, 539
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 3 cut(s) 128, 167, 208
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 3 cut(s) 128, 167, 208
Bsu15I ATCGAT 1 cut(s) 517
BsuRI GGCC 7 cut(s) 71, 75, 197, 266, 339, 457, 549
BsuTUI ATCGAT 1 cut(s) 517
BtgI CCRYGG 4 cut(s) 27, 261, 345, 544
BtsCI GGATG 1 cut(s) 355
BtsIMutI CAGTG 3 cut(s) 279, 366, 432
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 4 cut(s) 73, 90, 332, 597
CaiI CAGNNNCTG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 71
Cfr9I CCCGGG 1 cut(s) 510
ClaI ATCGAT 1 cut(s) 517
Csp6I GTAC 2 cut(s) 223, 304
CviAII CATG 7 cut(s) 28, 77, 262, 341, 346, 466, 545
CviQI GTAC 2 cut(s) 223, 304
DdeI CTNAG 3 cut(s) 95, 123, 603
DpnI GATC 6 cut(s) 130, 169, 210, 494, 516, 520
DpnII GATC 6 cut(s) 128, 167, 208, 492, 514, 518
DrdI GACNNNNNNGTC 1 cut(s) 283
DseDI GACNNNNNNGTC 1 cut(s) 283
EaeI YGGCCR 4 cut(s) 69, 73, 337, 547
EciI GGCGGA 1 cut(s) 558
Ecl136II GAGCTC 2 cut(s) 334, 599
Eco130I CCWWGG 4 cut(s) 27, 261, 345, 544
Eco24I GRGCYC 3 cut(s) 336, 501, 601
Eco53kI GAGCTC 2 cut(s) 334, 599
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 2 cut(s) 334, 599
EcoRII CCWGG 2 cut(s) 287, 480
EcoT14I CCWWGG 4 cut(s) 27, 261, 345, 544
EcoT38I GRGCYC 3 cut(s) 336, 501, 601
ErhI CCWWGG 4 cut(s) 27, 261, 345, 544
FaeI CATG 7 cut(s) 31, 80, 265, 344, 349, 469, 548
FatI CATG 7 cut(s) 27, 76, 261, 340, 345, 465, 544
FauI CCCGC 2 cut(s) 590, 600
Fnu4HI GCNGC 8 cut(s) 47, 310, 321, 538, 541, 550, 553, 572
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 3 cut(s) 336, 501, 601
FseI GGCCGGCC 1 cut(s) 75
Fsp4HI GCNGC 8 cut(s) 47, 310, 321, 538, 541, 550, 553, 572
GluI GCNGC 8 cut(s) 47, 310, 321, 538, 541, 550, 553, 572
GsaI CCCAGC 1 cut(s) 505
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 7 cut(s) 71, 75, 197, 266, 339, 457, 549
HapII CCGG 4 cut(s) 72, 458, 511, 526
Hin1II CATG 7 cut(s) 31, 80, 265, 344, 349, 469, 548
HinfI GANTC 1 cut(s) 156
HpaII CCGG 4 cut(s) 72, 458, 511, 526
HphI GGTGA 1 cut(s) 248
Hpy188I TCNGA 2 cut(s) 96, 126
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 2 cut(s) 274, 446
HpyCH4V TGCA 2 cut(s) 233, 323
HpyF10VI GCNNNNNNNGC 5 cut(s) 46, 336, 350, 546, 549
HpyF3I CTNAG 3 cut(s) 95, 123, 603
Hsp92II CATG 7 cut(s) 31, 80, 265, 344, 349, 469, 548
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 6 cut(s) 128, 167, 208, 492, 514, 518
LmnI GCTCC 3 cut(s) 37, 579, 604
Lsp1109I GCAGC 4 cut(s) 33, 307, 321, 539
LweI GCATC 2 cut(s) 220, 340
MaeIII GTNAC 3 cut(s) 157, 236, 274
MalI GATC 6 cut(s) 130, 169, 210, 494, 516, 520
MbiI CCGCTC 1 cut(s) 574
MboI GATC 6 cut(s) 128, 167, 208, 492, 514, 518
MboII GAAGA 2 cut(s) 131, 557
MflI RGATCY 3 cut(s) 128, 167, 208
MhlI GDGCHC 4 cut(s) 336, 368, 501, 601
MluCI AATT 2 cut(s) 8, 172
MluI ACGCGT 1 cut(s) 610
MlyI GAGTC 1 cut(s) 165
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 8 cut(s) 9, 45, 212, 409, 416, 454, 566, 612
MroNI GCCGGC 1 cut(s) 71
MspA1I CMGCKG 2 cut(s) 552, 620
MspI CCGG 4 cut(s) 72, 458, 511, 526
MspR9I CCNGG 5 cut(s) 289, 482, 511, 512, 527
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 289, 482
MvnI CGCG 2 cut(s) 595, 612
MwoI GCNNNNNNNGC 5 cut(s) 46, 336, 350, 546, 549
NaeI GCCGGC 1 cut(s) 73
NciI CCSGG 3 cut(s) 511, 512, 527
NcoI CCATGG 4 cut(s) 27, 261, 345, 544
NdeII GATC 6 cut(s) 128, 167, 208, 492, 514, 518
NgoMIV GCCGGC 1 cut(s) 71
NlaIII CATG 7 cut(s) 31, 80, 265, 344, 349, 469, 548
NlaIV GGNNCC 1 cut(s) 33
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 3 cut(s) 157, 236, 274
PaqCI CACCTGC 1 cut(s) 224
PctI GAATGC 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 73
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 8 cut(s) 48, 311, 322, 539, 542, 551, 554, 573
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp124BI GAGCTC 2 cut(s) 336, 601
Psp6I CCWGG 2 cut(s) 287, 480
PspFI CCCAGC 1 cut(s) 501
PspGI CCWGG 2 cut(s) 287, 480
PspN4I GGNNCC 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 3 cut(s) 128, 167, 208
PvuII CAGCTG 1 cut(s) 620
RigI GGCCGGCC 1 cut(s) 75
RsaI GTAC 2 cut(s) 224, 305
RsaNI GTAC 2 cut(s) 223, 304
SacI GAGCTC 2 cut(s) 336, 601
SatI GCNGC 8 cut(s) 47, 310, 321, 538, 541, 550, 553, 572
Sau3AI GATC 6 cut(s) 128, 167, 208, 492, 514, 518
SchI GAGTC 1 cut(s) 165
ScrFI CCNGG 5 cut(s) 289, 482, 511, 512, 527
SduI GDGCHC 4 cut(s) 336, 368, 501, 601
SetI ASST 6 cut(s) 124, 238, 336, 420, 601, 622
SfaNI GCATC 2 cut(s) 220, 340
SmaI CCCGGG 1 cut(s) 512
Sse9I AATT 2 cut(s) 8, 172
SsiI CCGC 9 cut(s) 478, 535, 538, 541, 550, 569, 572, 583, 593
SstI GAGCTC 2 cut(s) 336, 601
StyD4I CCNGG 5 cut(s) 287, 480, 509, 510, 525
StyI CCWWGG 4 cut(s) 27, 261, 345, 544
TaaI ACNGT 2 cut(s) 274, 446
TaqI TCGA 3 cut(s) 300, 495, 517
TasI AATT 2 cut(s) 8, 172
TauI GCSGC 4 cut(s) 540, 543, 552, 574
TscAI CASTG 3 cut(s) 279, 373, 439
TseFI GTSAC 3 cut(s) 157, 236, 274
TseI GCWGC 4 cut(s) 46, 309, 320, 552
Tsp45I GTSAC 3 cut(s) 157, 236, 274
TspDTI ATGAA 1 cut(s) 367
TspMI CCCGGG 1 cut(s) 510
TspRI CASTG 3 cut(s) 279, 373, 439
Van91I CCANNNNNTGG 1 cut(s) 346
XapI RAATTY 1 cut(s) 8
XmaI CCCGGG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.