pycom17g12680

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0019819
Physical Location & Seq
Forward (+)
10419442 .. 10420597
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g12680.1

Sequence Viewer

Length: 1107 bp
ATGGAGAAATTTGTGAGGGTAATTTCCATGGCTCCTCTGCTTCTGCTGCTCTGTTTGCCTTTTGCTTTGGCCGGCCATGACTACGGGCAGGCTCTGAGCAAGAGCATTCTGTTCTTGGAAGCTCAGAGATCTGGGTTTCTTCCCCGCAACCAGAGAGTCACTTGGAGATCTAATTCTGGCTTGTACGATGGTAAGGCCAATGGGGTGGATCTGGTGGGAGGGTACTATGATGCAGGTGACAATGTGAAGTTTGGGCTTCCCATGGCCTTCACAGTGACAATGATGTCCTGGAGTATAATCGAGTACGGCAAGCAAATGGCTGCAAGCGGTGAGCTCGGCCATGCCATGGAGGCCGTTAAATGGGGCACTGACTACTTCATCAAAGCTCATCCTGAACCCAATGTCCTCTACGGAGAGGTCGGAGATGGGAACATTGACCATTACTGTTGGCAAAGACCGGAGGACATGACCACTAACCGCCGGGCATACAAGATCAGCCCCAGCAATCCCGGGTCCGATTTCGCCGGAGAAACCGCCGCCGCCATGGCGGCTGCTTCCATTGTCTTCCGCCGCTCCAACCCCGCATACTCCCGTGAACTCCTCCGCCACGCCTATCAGCTGTTTGATTTTGCGGACAAGTACAGGGGTAAATATGACAGCAGCGCTCAATCTTCTTTTGGGGATTTGAAGAAAATGTTGGTTGGACCGACTGGTTTGCACCATCAAGATCATAAATTGATATTCAAAGACAAGGAGAGGGACTCGAAGGCTTTCCTCTACATGTCTGGGCGATATCTTGAGATGCGACGCAATGCCAAGATGGAAAGGGCTTCGAAGTCCATCTCCGAAATCAGCATTGAAGTAGACAGGCTTGCTGGCCAGGTGTCGGCTCTTGAATCAATTATCAATAAAGGTAAAAATGTTGCAGAGCAAGATGTGCTTGTTCTGATTGAGCAATTGATGAACCAGTTGCTTAAATTAGACGGGATCATGGGAGACGGAGAAGGCGATGTGCAATGGCTGTTGTTAGTGGCTGTGCTCAGTGATGGTAATGGTGGAGCTTTGGATTCCAGAGGTTCATCTTCCATGGTAGAACAAGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

369

Amino Acids

40.81

Weight (kDa)

5.96

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 223 7.7e-87 Glycosyl hydrolase family 9
BAG PF02179 281 - 338 2.1e-08 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019819)

Species Orthologous Gene IDs
pyrus_communis pycom06g10010 pycom17g12680
rosa_chinensis RchiOBHm_Chr0c20g0500181 RchiOBHm_Chr1g0340171
rosa_multiflora Rmu_sc0003311.1_g000003 Rmu_sc0032132.1_g000001
rosa_samantha Rh1CG166700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 283
Acc36I ACCTGC 1 cut(s) 224
AccB7I CCANNNNNTGG 1 cut(s) 346
AccBSI CCGCTC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 864
AclWI GGATC 2 cut(s) 216, 995
AcoI YGGCCR 4 cut(s) 69, 73, 337, 877
AcsI RAATTY 1 cut(s) 8
AfaI GTAC 4 cut(s) 185, 224, 305, 641
AfeI AGCGCT 1 cut(s) 664
AfiI CCNNNNNNNGG 3 cut(s) 346, 360, 886
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 4 cut(s) 688, 745, 860, 896
AjnI CCWGG 2 cut(s) 287, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
AluBI AGCT 5 cut(s) 122, 334, 386, 619, 1061
AluI AGCT 5 cut(s) 122, 334, 386, 619, 1061
Alw21I GWGCWC 2 cut(s) 336, 1041
Alw26I GTCTC 1 cut(s) 990
AlwI GGATC 2 cut(s) 216, 995
AlwNI CAGNNNCTG 1 cut(s) 94
Ama87I CYCGRG 1 cut(s) 509
Aor51HI AGCGCT 1 cut(s) 664
AoxI GGCC 7 cut(s) 69, 73, 195, 264, 337, 351, 877
ApeKI GCWGC 4 cut(s) 46, 320, 551, 660
ApoI RAATTY 1 cut(s) 8
Asp700I GAANNNNTTC 1 cut(s) 770
AspLEI GCGC 1 cut(s) 665
AspS9I GGNCC 2 cut(s) 513, 704
AsuC2I CCSGG 3 cut(s) 482, 510, 511
AsuHPI GGTGA 2 cut(s) 248, 341
AsuII TTCGAA 1 cut(s) 833
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 2 cut(s) 513, 704
BaeGI GKGCMC 1 cut(s) 368
BalI TGGCCA 1 cut(s) 879
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 2 cut(s) 336, 1041
BbvI GCAGC 4 cut(s) 33, 307, 538, 672
BccI CCATC 6 cut(s) 182, 419, 729, 814, 848, 1040
BceAI ACGGC 2 cut(s) 322, 338
BcgI CGANNNNNNTGC 2 cut(s) 697, 731
BciT130I CCWGG 2 cut(s) 289, 881
BcnI CCSGG 3 cut(s) 482, 510, 511
BcoDI GTCTC 1 cut(s) 990
BfoI RGCGCY 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 224
BglI GCCNNNNNGGC 3 cut(s) 350, 545, 548
BglII AGATCT 2 cut(s) 128, 167
BisI GCNGC 8 cut(s) 47, 321, 537, 540, 549, 552, 571, 661
BlsI GCNGC 8 cut(s) 48, 322, 538, 541, 550, 553, 572, 662
Bme1390I CCNGG 5 cut(s) 289, 482, 510, 511, 881
Bme18I GGWCC 2 cut(s) 513, 704
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 2 cut(s) 513, 704
BmiI GGNNCC 2 cut(s) 33, 514
BmrFI CCNGG 5 cut(s) 289, 482, 510, 511, 881
BmsI GCATC 2 cut(s) 220, 792
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 746, 778
BpmI CTGGAG 1 cut(s) 310
Bpu14I TTCGAA 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 3 cut(s) 482, 510, 511
BsaJI CCNNGG 6 cut(s) 27, 261, 345, 509, 543, 1086
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 360, 886
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 2 cut(s) 715, 967
Bse3DI GCAATG 2 cut(s) 817, 1022
BseBI CCWGG 2 cut(s) 289, 881
BseDI CCNNGG 6 cut(s) 27, 261, 345, 509, 543, 1086
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 3 cut(s) 346, 360, 886
BseMI GCAATG 2 cut(s) 817, 1022
BseMII CTCAG 3 cut(s) 86, 137, 1054
BseNI ACTGG 2 cut(s) 715, 967
BseRI GAGGAG 2 cut(s) 24, 590
BseSI GKGCMC 1 cut(s) 368
BseXI GCAGC 4 cut(s) 33, 307, 538, 672
BseYI CCCAGC 1 cut(s) 500
BshFI GGCC 7 cut(s) 71, 75, 197, 266, 339, 353, 879
BsiHKAI GWGCWC 2 cut(s) 336, 1041
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 5 cut(s) 72, 458, 481, 510, 525
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 3 cut(s) 346, 360, 886
BsmAI GTCTC 1 cut(s) 990
BsmBI CGTCTC 1 cut(s) 990
BsmFI GGGAC 1 cut(s) 773
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 7 cut(s) 71, 75, 197, 266, 339, 353, 879
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 336, 368, 1041
Bsp143I GATC 6 cut(s) 128, 167, 208, 492, 727, 987
Bsp19I CCATGG 5 cut(s) 27, 261, 345, 543, 1086
BspANI GGCC 7 cut(s) 71, 75, 197, 266, 339, 353, 879
BspCNI CTCAG 3 cut(s) 87, 136, 1053
BspLI GGNNCC 2 cut(s) 33, 514
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 2 cut(s) 216, 995
BspT104I TTCGAA 1 cut(s) 833
BsrBI CCGCTC 1 cut(s) 573
BsrDI GCAATG 2 cut(s) 817, 1022
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 2 cut(s) 715, 967
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 6 cut(s) 27, 261, 345, 509, 543, 1086
BssMI GATC 6 cut(s) 128, 167, 208, 492, 727, 987
BssT1I CCWWGG 5 cut(s) 27, 261, 345, 543, 1086
Bst2UI CCWGG 2 cut(s) 289, 881
Bst4CI ACNGT 2 cut(s) 274, 446
BstAPI GCANNNNNTGC 1 cut(s) 937
BstBI TTCGAA 1 cut(s) 833
BstC8I GCNNGC 6 cut(s) 73, 90, 311, 325, 873, 877
BstDEI CTNAG 3 cut(s) 95, 123, 1040
BstDSI CCRYGG 5 cut(s) 27, 261, 345, 543, 1086
BstF5I GGATG 1 cut(s) 388
BstH2I RGCGCY 1 cut(s) 666
BstHHI GCGC 1 cut(s) 665
BstKTI GATC 6 cut(s) 131, 170, 211, 495, 730, 990
BstMAI GTCTC 1 cut(s) 990
BstMBI GATC 6 cut(s) 128, 167, 208, 492, 727, 987
BstMWI GCNNNNNNNGC 6 cut(s) 46, 55, 350, 545, 548, 937
BstNI CCWGG 2 cut(s) 289, 881
BstNSI RCATGY 1 cut(s) 784
BstSCI CCNGG 5 cut(s) 287, 480, 508, 509, 879
BstSLI GKGCMC 1 cut(s) 368
BstV1I GCAGC 4 cut(s) 33, 307, 538, 672
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 3 cut(s) 128, 167, 208
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 3 cut(s) 128, 167, 208
BsuRI GGCC 7 cut(s) 71, 75, 197, 266, 339, 353, 879
BtgI CCRYGG 5 cut(s) 27, 261, 345, 543, 1086
BtgZI GCGATG 1 cut(s) 1023
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 3 cut(s) 279, 366, 1048
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 6 cut(s) 73, 90, 311, 325, 873, 877
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 1 cut(s) 665
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 2 cut(s) 513, 704
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 1 cut(s) 816
Csp6I GTAC 4 cut(s) 184, 223, 304, 640
CviQI GTAC 4 cut(s) 184, 223, 304, 640
DdeI CTNAG 3 cut(s) 95, 123, 1040
DpnI GATC 6 cut(s) 130, 169, 210, 494, 729, 989
DpnII GATC 6 cut(s) 128, 167, 208, 492, 727, 987
DrdI GACNNNNNNGTC 1 cut(s) 283
DseDI GACNNNNNNGTC 1 cut(s) 283
EaeI YGGCCR 4 cut(s) 69, 73, 337, 877
EciI GGCGGA 2 cut(s) 557, 593
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 5 cut(s) 27, 261, 345, 543, 1086
Eco24I GRGCYC 1 cut(s) 336
Eco32I GATATC 1 cut(s) 794
Eco47I GGWCC 2 cut(s) 513, 704
Eco47III AGCGCT 1 cut(s) 664
Eco53kI GAGCTC 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 287, 879
EcoRV GATATC 1 cut(s) 794
EcoT14I CCWWGG 5 cut(s) 27, 261, 345, 543, 1086
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 5 cut(s) 27, 261, 345, 543, 1086
Esp3I CGTCTC 1 cut(s) 990
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FaqI GGGAC 1 cut(s) 773
FauI CCCGC 2 cut(s) 152, 589
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 8 cut(s) 47, 321, 537, 540, 549, 552, 571, 661
FokI GGATG 1 cut(s) 375
FriOI GRGCYC 1 cut(s) 336
FseI GGCCGGCC 1 cut(s) 75
Fsp4HI GCNGC 8 cut(s) 47, 321, 537, 540, 549, 552, 571, 661
GlaI GCGC 1 cut(s) 664
GluI GCNGC 8 cut(s) 47, 321, 537, 540, 549, 552, 571, 661
GsaI CCCAGC 1 cut(s) 504
GsuI CTGGAG 1 cut(s) 310
HaeII RGCGCY 1 cut(s) 666
HaeIII GGCC 7 cut(s) 71, 75, 197, 266, 339, 353, 879
HapII CCGG 5 cut(s) 72, 458, 481, 510, 525
HgaI GACGC 1 cut(s) 816
HhaI GCGC 1 cut(s) 665
Hin6I GCGC 1 cut(s) 663
HinP1I GCGC 1 cut(s) 663
HinfI GANTC 4 cut(s) 156, 761, 896, 1067
HpaII CCGG 5 cut(s) 72, 458, 481, 510, 525
HphI GGTGA 2 cut(s) 248, 341
Hpy166II GTNNAC 2 cut(s) 596, 865
Hpy188I TCNGA 6 cut(s) 96, 126, 422, 517, 847, 948
Hpy188III TCNNGA 5 cut(s) 392, 725, 797, 893, 1071
Hpy8I GTNNAC 2 cut(s) 596, 865
Hpy99I CGWCG 1 cut(s) 810
HpyAV CCTTC 3 cut(s) 277, 760, 998
HpyCH4III ACNGT 2 cut(s) 274, 446
HpyCH4V TGCA 5 cut(s) 233, 323, 718, 926, 1015
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 55, 350, 545, 548, 937
HpyF3I CTNAG 3 cut(s) 95, 123, 1040
HspAI GCGC 1 cut(s) 663
KroI GCCGGC 1 cut(s) 71
KroNI GCCGGC 1 cut(s) 73
Kzo9I GATC 6 cut(s) 128, 167, 208, 492, 727, 987
LmnI GCTCC 3 cut(s) 37, 578, 1058
Lsp1109I GCAGC 4 cut(s) 33, 307, 538, 672
LweI GCATC 2 cut(s) 220, 792
MaeIII GTNAC 3 cut(s) 157, 236, 274
MalI GATC 6 cut(s) 130, 169, 210, 494, 729, 989
MbiI CCGCTC 1 cut(s) 573
MboI GATC 6 cut(s) 128, 167, 208, 492, 727, 987
MboII GAAGA 5 cut(s) 131, 556, 663, 700, 1074
MfeI CAATTG 1 cut(s) 956
MflI RGATCY 3 cut(s) 128, 167, 208
MhlI GDGCHC 3 cut(s) 336, 368, 1041
MlsI TGGCCA 1 cut(s) 879
MluCI AATT 7 cut(s) 8, 21, 172, 734, 900, 956, 977
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 2 cut(s) 165, 755
MmeI TCCRAC 3 cut(s) 400, 600, 682
Mox20I TGGCCA 1 cut(s) 879
MroNI GCCGGC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 770
MscI TGGCCA 1 cut(s) 879
MseI TTAA 2 cut(s) 357, 975
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 5 cut(s) 72, 458, 481, 510, 525
MspR9I CCNGG 5 cut(s) 289, 482, 510, 511, 881
MunI CAATTG 1 cut(s) 956
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 289, 881
MwoI GCNNNNNNNGC 6 cut(s) 46, 55, 350, 545, 548, 937
NaeI GCCGGC 1 cut(s) 73
NciI CCSGG 3 cut(s) 482, 510, 511
NcoI CCATGG 5 cut(s) 27, 261, 345, 543, 1086
NdeII GATC 6 cut(s) 128, 167, 208, 492, 727, 987
NgoMIV GCCGGC 1 cut(s) 71
NlaIV GGNNCC 2 cut(s) 33, 514
NmeAIII GCCGAG 1 cut(s) 315
NmuCI GTSAC 3 cut(s) 157, 236, 274
NspI RCATGY 1 cut(s) 784
NspV TTCGAA 1 cut(s) 833
PaqCI CACCTGC 1 cut(s) 224
PciI ACATGT 1 cut(s) 780
PcsI WCGNNNNNNNCGW 1 cut(s) 1005
PctI GAATGC 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 770
PfeI GAWTC 2 cut(s) 896, 1067
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 287
PkrI GCNGC 8 cut(s) 48, 322, 538, 541, 550, 553, 572, 662
PleI GAGTC 2 cut(s) 164, 755
PpsI GAGTC 2 cut(s) 164, 755
PscI ACATGT 1 cut(s) 780
Psp124BI GAGCTC 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 287, 879
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 2 cut(s) 287, 879
PspN4I GGNNCC 2 cut(s) 33, 514
PspPI GGNCC 2 cut(s) 513, 704
PstNI CAGNNNCTG 1 cut(s) 94
PsuI RGATCY 3 cut(s) 128, 167, 208
PvuII CAGCTG 1 cut(s) 619
RigI GGCCGGCC 1 cut(s) 75
RsaI GTAC 4 cut(s) 185, 224, 305, 641
RsaNI GTAC 4 cut(s) 184, 223, 304, 640
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 357, 975
SatI GCNGC 8 cut(s) 47, 321, 537, 540, 549, 552, 571, 661
Sau3AI GATC 6 cut(s) 128, 167, 208, 492, 727, 987
Sau96I GGNCC 2 cut(s) 513, 704
SchI GAGTC 2 cut(s) 165, 755
ScrFI CCNGG 5 cut(s) 289, 482, 510, 511, 881
SduI GDGCHC 3 cut(s) 336, 368, 1041
SfaNI GCATC 2 cut(s) 220, 792
SfuI TTCGAA 1 cut(s) 833
SinI GGWCC 2 cut(s) 513, 704
SmaI CCCGGG 1 cut(s) 511
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 7 cut(s) 8, 21, 172, 734, 900, 956, 977
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 5 cut(s) 287, 480, 508, 509, 879
StyI CCWWGG 5 cut(s) 27, 261, 345, 543, 1086
TaaI ACNGT 2 cut(s) 274, 446
TaqI TCGA 3 cut(s) 300, 764, 833
TaqII GACCGA 1 cut(s) 721
TasI AATT 7 cut(s) 8, 21, 172, 734, 900, 956, 977
TatI WGTACW 1 cut(s) 639
TauI GCSGC 4 cut(s) 539, 542, 551, 573
TfiI GAWTC 2 cut(s) 896, 1067
Tru1I TTAA 2 cut(s) 357, 975
Tru9I TTAA 2 cut(s) 357, 975
TscAI CASTG 3 cut(s) 279, 373, 1048
TseFI GTSAC 3 cut(s) 157, 236, 274
TseI GCWGC 4 cut(s) 46, 320, 551, 660
Tsp45I GTSAC 3 cut(s) 157, 236, 274
TspDTI ATGAA 3 cut(s) 367, 977, 1068
TspGWI ACGGA 2 cut(s) 426, 1014
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 3 cut(s) 279, 373, 1048
Van91I CCANNNNNTGG 1 cut(s) 346
VpaK11BI GGWCC 2 cut(s) 513, 704
XapI RAATTY 1 cut(s) 8
XceI RCATGY 1 cut(s) 784
XmaI CCCGGG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 864
XmnI GAANNNNTTC 1 cut(s) 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.