RchiOBHm_Chr1g0340171

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0019819
Physical Location & Seq
Forward (+)
31717488 .. 31721500
4013 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56698

Sequence Viewer

Length: 711 bp
ATGGCTTCTATCCATGTCCTTGGTTTGGTTTGCTTTTCTCTCTTTTTATCAACTCATTCAGTGCTTGCGGCGATAGATTATGGACAAGCTCTTACAAAGTCTTTGTTGTATTATGAGGCTCAACGATCTGGTAAGTTGCCTCCTGAACAGAGAGTGCAATGGCGTGGCGATTCAGCGCTTAAAGATGGAAATGATACTGGTGTTAATCTTGATGGAGGGTATTATGATTCTGGGGACAATGTCAAGTTTGGATTCCCCATGGCATTCACAATAACAATGCTTTCATGGAGCACAATAGAGTTCGCAAGCAAACTTGAAACCAAGAATGAGCTCTCAAACGCATTGGACGCCATTAAATGGGGCACAGACTATTTGATCAAAGCACACGCTCAGCCAAATGTTCTCTATGGTGAAGTTGGCGATGGCGGTTCTGATCATGCTTGTTGGCAGAGACCGGAAGACATGACTACCCCACGGACATCCTTCAAGATCGATGAGCAGCATCCAGGTTCTGATCTTGCTGGTGAAACCGCAGCTGCTTTTGCCGCAGCTTCCATTGCTTTCAAGGATAAAGACCCTACATATTCTGGCCAGCTCTTGACGCATGCGAAACAGCTGTTTGAATTTGCCTGCAGTCATGCTGGTGTTTATCAGAATAGTATTAATCTAGCAGGCCAGTTCTACTCCAGCAGCGGATATGAGGTAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

236

Amino Acids

25.73

Weight (kDa)

5.04

Isoelectric Point (pI)

24.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 27 - 233 7.8e-89 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019819)

Species Orthologous Gene IDs
pyrus_communis pycom06g10010 pycom17g12680
rosa_chinensis RchiOBHm_Chr0c20g0500181 RchiOBHm_Chr1g0340171
rosa_multiflora Rmu_sc0003311.1_g000003 Rmu_sc0032132.1_g000001
rosa_samantha Rh1CG166700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 357
AciI CCGC 5 cut(s) 68, 426, 531, 546, 693
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 623
AcyI GRCGYC 1 cut(s) 348
AfeI AGCGCT 1 cut(s) 177
AfiI CCNNNNNNNGG 2 cut(s) 25, 357
AgsI TTSAA 4 cut(s) 317, 487, 565, 623
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 6 cut(s) 89, 331, 536, 551, 595, 616
AluI AGCT 6 cut(s) 89, 331, 536, 551, 595, 616
Alw21I GWGCWC 2 cut(s) 293, 333
Alw26I GTCTC 1 cut(s) 445
AlwNI CAGNNNCTG 1 cut(s) 512
Aor51HI AGCGCT 1 cut(s) 177
AoxI GGCC 2 cut(s) 589, 673
ApeKI GCWGC 5 cut(s) 499, 533, 536, 548, 690
ApoI RAATTY 1 cut(s) 623
AseI ATTAAT 1 cut(s) 663
AspLEI GCGC 1 cut(s) 178
AsuHPI GGTGA 2 cut(s) 422, 536
BaeGI GKGCMC 1 cut(s) 365
BaeI ACNNNNGTAYC 2 cut(s) 186, 219
BalI TGGCCA 1 cut(s) 591
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 2 cut(s) 293, 333
BbvI GCAGC 5 cut(s) 511, 523, 545, 560, 702
BccI CCATC 3 cut(s) 179, 206, 416
BciT130I CCWGG 1 cut(s) 507
BclI TGATCA 2 cut(s) 375, 433
BcoDI GTCTC 1 cut(s) 445
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 631
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 7 cut(s) 69, 500, 534, 537, 546, 549, 691
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 7 cut(s) 70, 501, 535, 538, 547, 550, 692
Bme1390I CCNGG 1 cut(s) 507
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 465
BpmI CTGGAG 1 cut(s) 670
Bpu1102I GCTNAGC 1 cut(s) 390
Bsa29I ATCGAT 1 cut(s) 492
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 445
BsaJI CCNNGG 3 cut(s) 19, 258, 473
BsaWI WCCGGW 1 cut(s) 454
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 357
Bse1I ACTGG 2 cut(s) 202, 676
Bse3DI GCAATG 2 cut(s) 164, 555
BseBI CCWGG 1 cut(s) 507
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 19, 258, 473
BseGI GGATG 2 cut(s) 479, 502
BseLI CCNNNNNNNGG 2 cut(s) 25, 357
BseMI GCAATG 2 cut(s) 164, 555
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 2 cut(s) 202, 676
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 5 cut(s) 511, 523, 545, 560, 702
BshFI GGCC 2 cut(s) 591, 675
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 293, 333
BsiSI CCGG 1 cut(s) 455
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 2 cut(s) 25, 357
BsmAI GTCTC 1 cut(s) 445
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 2 cut(s) 591, 675
Bso31I GGTCTC 1 cut(s) 445
Bsp1286I GDGCHC 3 cut(s) 293, 333, 365
Bsp143I GATC 5 cut(s) 125, 375, 433, 489, 514
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 5 cut(s) 68, 426, 531, 546, 693
BspANI GGCC 2 cut(s) 591, 675
BspCNI CTCAG 1 cut(s) 403
BspDI ATCGAT 1 cut(s) 492
BspMAI CTGCAG 1 cut(s) 635
BspTNI GGTCTC 1 cut(s) 445
BsrDI GCAATG 2 cut(s) 164, 555
BsrI ACTGG 2 cut(s) 202, 676
BssECI CCNNGG 3 cut(s) 19, 258, 473
BssMI GATC 5 cut(s) 125, 375, 433, 489, 514
BssNI GRCGYC 1 cut(s) 348
BssT1I CCWWGG 2 cut(s) 19, 258
Bst2UI CCWGG 1 cut(s) 507
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 6 cut(s) 66, 307, 593, 606, 631, 673
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 2 cut(s) 258, 473
BstF5I GGATG 2 cut(s) 479, 502
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 5 cut(s) 128, 378, 436, 492, 517
BstMAI GTCTC 1 cut(s) 445
BstMBI GATC 5 cut(s) 125, 375, 433, 489, 514
BstMWI GCNNNNNNNGC 5 cut(s) 347, 542, 545, 557, 601
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 1 cut(s) 505
BstSFI CTRYAG 1 cut(s) 631
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 5 cut(s) 511, 523, 545, 560, 702
BstV2I GAAGAC 1 cut(s) 465
BstXI CCANNNNNNTGG 1 cut(s) 20
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 2 cut(s) 591, 675
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 2 cut(s) 258, 473
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 2 cut(s) 479, 502
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 6 cut(s) 66, 307, 593, 606, 631, 673
CaiI CAGNNNCTG 1 cut(s) 512
CfoI GCGC 1 cut(s) 178
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 2 cut(s) 356, 610
CviAII CATG 7 cut(s) 14, 259, 285, 437, 463, 605, 638
DdeI CTNAG 1 cut(s) 390
DpnI GATC 5 cut(s) 127, 377, 435, 491, 516
DpnII GATC 5 cut(s) 125, 375, 433, 489, 514
EaeI YGGCCR 1 cut(s) 589
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 19, 258
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 445
Eco47III AGCGCT 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 2 cut(s) 19, 258
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 19, 258
FaeI CATG 7 cut(s) 17, 262, 288, 440, 466, 608, 641
FaqI GGGAC 1 cut(s) 248
FatI CATG 7 cut(s) 13, 258, 284, 436, 462, 604, 637
FbaI TGATCA 2 cut(s) 375, 433
Fnu4HI GCNGC 7 cut(s) 69, 500, 534, 537, 546, 549, 691
FokI GGATG 2 cut(s) 466, 489
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 7 cut(s) 69, 500, 534, 537, 546, 549, 691
FspBI CTAG 1 cut(s) 668
GlaI GCGC 1 cut(s) 177
GluI GCNGC 7 cut(s) 69, 500, 534, 537, 546, 549, 691
GsuI CTGGAG 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 591, 675
HapII CCGG 1 cut(s) 455
HgaI GACGC 2 cut(s) 356, 610
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 7 cut(s) 17, 262, 288, 440, 466, 608, 641
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HinfI GANTC 3 cut(s) 170, 227, 252
HpaII CCGG 1 cut(s) 455
HphI GGTGA 2 cut(s) 422, 536
Hpy188I TCNGA 3 cut(s) 433, 514, 654
Hpy188III TCNNGA 4 cut(s) 143, 209, 487, 598
HpyAV CCTTC 1 cut(s) 493
HpyCH4V TGCA 2 cut(s) 157, 633
HpyF10VI GCNNNNNNNGC 5 cut(s) 347, 542, 545, 557, 601
HpyF3I CTNAG 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 7 cut(s) 17, 262, 288, 440, 466, 608, 641
HspAI GCGC 1 cut(s) 176
Ksp22I TGATCA 2 cut(s) 375, 433
Kzo9I GATC 5 cut(s) 125, 375, 433, 489, 514
LmnI GCTCC 1 cut(s) 288
Lsp1109I GCAGC 5 cut(s) 511, 523, 545, 560, 702
LweI GCATC 1 cut(s) 511
MaeI CTAG 1 cut(s) 668
MalI GATC 5 cut(s) 127, 377, 435, 491, 516
MboI GATC 5 cut(s) 125, 375, 433, 489, 514
MboII GAAGA 1 cut(s) 470
MhlI GDGCHC 3 cut(s) 293, 333, 365
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 1 cut(s) 623
MluNI TGGCCA 1 cut(s) 591
MnlI CCTC 4 cut(s) 109, 150, 209, 694
Mox20I TGGCCA 1 cut(s) 591
MscI TGGCCA 1 cut(s) 591
MseI TTAA 4 cut(s) 180, 204, 354, 663
MslI CAYNNNNRTG 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 591
MspA1I CMGCKG 3 cut(s) 536, 616, 693
MspI CCGG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 507
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 507
MwoI GCNNNNNNNGC 5 cut(s) 347, 542, 545, 557, 601
NcoI CCATGG 1 cut(s) 258
NdeII GATC 5 cut(s) 125, 375, 433, 489, 514
NlaIII CATG 7 cut(s) 17, 262, 288, 440, 466, 608, 641
NspI RCATGY 1 cut(s) 608
PaeI GCATGC 1 cut(s) 608
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 3 cut(s) 170, 227, 252
PflFI GACNNNGTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 357
PkrI GCNGC 7 cut(s) 70, 501, 535, 538, 547, 550, 692
PshBI ATTAAT 1 cut(s) 663
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PstI CTGCAG 1 cut(s) 635
PstNI CAGNNNCTG 1 cut(s) 512
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 2 cut(s) 536, 616
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 4 cut(s) 180, 204, 354, 663
SatI GCNGC 7 cut(s) 69, 500, 534, 537, 546, 549, 691
Sau3AI GATC 5 cut(s) 125, 375, 433, 489, 514
ScrFI CCNGG 1 cut(s) 507
SduI GDGCHC 3 cut(s) 293, 333, 365
SetI ASST 8 cut(s) 91, 333, 511, 538, 553, 597, 618, 705
SfaNI GCATC 1 cut(s) 511
SfcI CTRYAG 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 642
SphI GCATGC 1 cut(s) 608
Sse9I AATT 1 cut(s) 623
SsiI CCGC 5 cut(s) 68, 426, 531, 546, 693
SspMI CTAG 1 cut(s) 668
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 2 cut(s) 19, 258
TaqI TCGA 1 cut(s) 492
TasI AATT 1 cut(s) 623
TauI GCSGC 2 cut(s) 71, 548
TfiI GAWTC 3 cut(s) 170, 227, 252
Tru1I TTAA 4 cut(s) 180, 204, 354, 663
Tru9I TTAA 4 cut(s) 180, 204, 354, 663
TscAI CASTG 1 cut(s) 66
TseI GCWGC 5 cut(s) 499, 533, 536, 548, 690
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 239
Van91I CCANNNNNTGG 1 cut(s) 357
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 1 cut(s) 623
XceI RCATGY 1 cut(s) 608
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.