Rmu_sc0032132.1_g000001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0032132.1
OG0019819
Physical Location & Seq
Forward (+)
1 .. 706
706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0032132.1_g000001.1.cds

Sequence Viewer

Length: 510 bp
gttgatcttaatggagggtattatgatgctggggacaatgtgaagtttggattccccatggcattcacaataacaatgctttcatggagcacaatagagtttgcaagcaaacttgagaccaagaatgagctctccaacgcattggacgccattaaatggggaacagactatttgatcaaagctcaccctcagccaaatgtgctctatggcgaagttggcgatggcggttctgatcatacttgttggcagagaccggaagacatgactaccccacggacatccttcaagatcgatgagcagcatccaggttctgatcttgctggtgaaaccgcagctgcttttgccgcagcttccattgctttcaaggataaagaccctacatattctggcaagctcttgacgcatgcaaaacagctgtttgaatttgcccgcagtcatgctggtgtttatcagaatagtattgatgtagcaggccagttctactccagcagcggatatgaggtgatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
Glyco_hydro_9

Protein Analysis

169

Amino Acids

18.51

Weight (kDa)

4.85

Isoelectric Point (pI)

19.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019819)

Species Orthologous Gene IDs
pyrus_communis pycom06g10010 pycom17g12680
rosa_chinensis RchiOBHm_Chr0c20g0500181 RchiOBHm_Chr1g0340171
rosa_multiflora Rmu_sc0003311.1_g000003 Rmu_sc0032132.1_g000001
rosa_samantha Rh1CG166700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 156
AciI CCGC 5 cut(s) 225, 330, 345, 430, 492
AcsI RAATTY 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 3 cut(s) 286, 364, 422
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 6 cut(s) 130, 182, 335, 350, 394, 415
AluI AGCT 6 cut(s) 130, 182, 335, 350, 394, 415
Alw21I GWGCWC 3 cut(s) 92, 132, 204
Alw26I GTCTC 2 cut(s) 110, 244
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 5 cut(s) 298, 332, 335, 347, 489
ApoI RAATTY 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 176, 335
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 3 cut(s) 92, 132, 204
BbvCI CCTCAGC 1 cut(s) 189
BbvI GCAGC 5 cut(s) 310, 322, 344, 359, 501
BccI CCATC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 306
BclI TGATCA 2 cut(s) 174, 232
BcoDI GTCTC 2 cut(s) 110, 244
BisI GCNGC 6 cut(s) 299, 333, 336, 345, 348, 490
BlsI GCNGC 6 cut(s) 300, 334, 337, 346, 349, 491
Bme1390I CCNGG 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 16, 310
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 469
Bpu10I CCTNAGC 1 cut(s) 189
BpuEI CTTGAG 1 cut(s) 134
Bsa29I ATCGAT 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 2 cut(s) 110, 244
BsaJI CCNNGG 2 cut(s) 57, 272
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 1 cut(s) 475
Bse3DI GCAATG 1 cut(s) 354
BseBI CCWGG 1 cut(s) 306
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 57, 272
BseGI GGATG 2 cut(s) 278, 301
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 475
BseXI GCAGC 5 cut(s) 310, 322, 344, 359, 501
BseYI CCCAGC 1 cut(s) 29
BshFI GGCC 1 cut(s) 474
BshVI ATCGAT 1 cut(s) 291
BsiHKAI GWGCWC 3 cut(s) 92, 132, 204
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 2 cut(s) 110, 244
BsmFI GGGAC 1 cut(s) 47
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 2 cut(s) 110, 244
Bsp1286I GDGCHC 3 cut(s) 92, 132, 204
Bsp143I GATC 5 cut(s) 4, 174, 232, 288, 313
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 5 cut(s) 225, 330, 345, 430, 492
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 1 cut(s) 202
BspDI ATCGAT 1 cut(s) 291
BspTNI GGTCTC 2 cut(s) 110, 244
BsrDI GCAATG 1 cut(s) 354
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 57, 272
BssMI GATC 5 cut(s) 4, 174, 232, 288, 313
BssNI GRCGYC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 147
BstC8I GCNNGC 5 cut(s) 106, 392, 405, 430, 472
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 2 cut(s) 57, 272
BstF5I GGATG 2 cut(s) 278, 301
BstKTI GATC 5 cut(s) 7, 177, 235, 291, 316
BstMAI GTCTC 2 cut(s) 110, 244
BstMBI GATC 5 cut(s) 4, 174, 232, 288, 313
BstMWI GCNNNNNNNGC 7 cut(s) 146, 199, 216, 341, 344, 356, 400
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 304
BstV1I GCAGC 5 cut(s) 310, 322, 344, 359, 501
BstV2I GAAGAC 1 cut(s) 264
BstXI CCANNNNNNTGG 1 cut(s) 142
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 291
BtgI CCRYGG 2 cut(s) 57, 272
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 2 cut(s) 278, 301
Cac8I GCNNGC 5 cut(s) 106, 392, 405, 430, 472
CaiI CAGNNNCTG 1 cut(s) 311
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 2 cut(s) 155, 409
CviAII CATG 5 cut(s) 58, 84, 262, 404, 437
CviJI RGCY 8 cut(s) 130, 182, 193, 335, 350, 394, 415, 474
CviKI_1 RGCY 8 cut(s) 130, 182, 193, 335, 350, 394, 415, 474
DdeI CTNAG 1 cut(s) 189
DpnI GATC 5 cut(s) 6, 176, 234, 290, 315
DpnII GATC 5 cut(s) 4, 174, 232, 288, 313
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 132
Eco31I GGTCTC 2 cut(s) 110, 244
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 5 cut(s) 61, 87, 265, 407, 440
FaqI GGGAC 1 cut(s) 47
FatI CATG 5 cut(s) 57, 83, 261, 403, 436
FauI CCCGC 1 cut(s) 437
FbaI TGATCA 2 cut(s) 174, 232
Fnu4HI GCNGC 6 cut(s) 299, 333, 336, 345, 348, 490
FokI GGATG 2 cut(s) 265, 288
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 6 cut(s) 299, 333, 336, 345, 348, 490
GluI GCNGC 6 cut(s) 299, 333, 336, 345, 348, 490
GsaI CCCAGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 469
HaeIII GGCC 1 cut(s) 474
HapII CCGG 1 cut(s) 254
HgaI GACGC 2 cut(s) 155, 409
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 5 cut(s) 61, 87, 265, 407, 440
HinfI GANTC 1 cut(s) 51
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 176, 335
Hpy188I TCNGA 3 cut(s) 232, 313, 453
Hpy188III TCNNGA 2 cut(s) 286, 397
HpyAV CCTTC 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 104, 407
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 199, 216, 341, 344, 356, 400
HpyF3I CTNAG 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 5 cut(s) 61, 87, 265, 407, 440
Ksp22I TGATCA 2 cut(s) 174, 232
Kzo9I GATC 5 cut(s) 4, 174, 232, 288, 313
LmnI GCTCC 1 cut(s) 87
Lsp1109I GCAGC 5 cut(s) 310, 322, 344, 359, 501
LweI GCATC 2 cut(s) 16, 310
MalI GATC 5 cut(s) 6, 176, 234, 290, 315
MboI GATC 5 cut(s) 4, 174, 232, 288, 313
MboII GAAGA 1 cut(s) 269
MhlI GDGCHC 3 cut(s) 92, 132, 204
MluCI AATT 1 cut(s) 422
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 3 cut(s) 8, 198, 493
MseI TTAA 2 cut(s) 9, 153
MslI CAYNNNNRTG 1 cut(s) 441
MspA1I CMGCKG 3 cut(s) 335, 415, 492
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 7 cut(s) 146, 199, 216, 341, 344, 356, 400
NcoI CCATGG 1 cut(s) 57
NdeII GATC 5 cut(s) 4, 174, 232, 288, 313
NlaIII CATG 5 cut(s) 61, 87, 265, 407, 440
NspI RCATGY 1 cut(s) 407
PaeI GCATGC 1 cut(s) 407
PctI GAATGC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 51
PflMI CCANNNNNTGG 1 cut(s) 156
PkrI GCNGC 6 cut(s) 300, 334, 337, 346, 349, 491
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 311
PvuII CAGCTG 2 cut(s) 335, 415
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 9, 153
SatI GCNGC 6 cut(s) 299, 333, 336, 345, 348, 490
Sau3AI GATC 5 cut(s) 4, 174, 232, 288, 313
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 3 cut(s) 92, 132, 204
SetI ASST 8 cut(s) 132, 184, 310, 337, 352, 396, 417, 504
SfaNI GCATC 2 cut(s) 16, 310
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 1 cut(s) 407
Sse9I AATT 1 cut(s) 422
SsiI CCGC 5 cut(s) 225, 330, 345, 430, 492
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 304
StyI CCWWGG 1 cut(s) 57
TaqI TCGA 1 cut(s) 291
TasI AATT 1 cut(s) 422
TauI GCSGC 1 cut(s) 347
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 2 cut(s) 9, 153
Tru9I TTAA 2 cut(s) 9, 153
TseI GCWGC 5 cut(s) 298, 332, 335, 347, 489
TspDTI ATGAA 1 cut(s) 72
TspGWI ACGGA 1 cut(s) 289
Van91I CCANNNNNTGG 1 cut(s) 156
XapI RAATTY 1 cut(s) 422
XceI RCATGY 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.