pycom06g10020

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008244
Physical Location & Seq
Forward (+)
15355521 .. 15356555
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 717 bp
ATGGGAGGAACCGGTTGGAGCATGACCGAGTTCGGTTGGGATGTCAAGTATCCCGGTGTCCAAACCCTTGTTGCCAAGTTTTTAATGCAAGGCAAAGCAGGTAGTCATACAGCAGTGTTTCAGAAGTACCAACAGAAGGCTGAGTACTTCATGTGTTCATGCGTTGGTAAGGGCAGCCAAAACGCACAGAAGACTCCCGGTGGCCTAATCTACCGCCAGAGGTGGAATAACATGCAGTTTGTCACCAGTGCCTCCTTCTTGGCCACCGTCTACTCCGACTATCTCACATCTTCCAGGAGTACGCTTCAGTGTGCTTCTGGCAATGTGGCACCCAACGAGCTCCTATCTTTCGCCCAATCTCAGGTGGATTACATTCTTGGAGATAACCCGAGAGCCACTAGTTACATGGTTGGCTACGGAAACAACTACCCTCAACAAGTCCACCACAGGGCTTCCTCAATTGTTTCCTACAAGAAGGACTCAACATTTGTGAGCTGCAGGGGAGGCTATGCCACTTGGTTTAGCCGGAAAGCTAGTGACCCCAATCTGCTTACTGGCGCTATTGTCGGTGGACCTGATGCCTATGACAACTTTGCTGATCAGAGAGACAACTATGAGCAGACAGAGCCTGCTACCTACAACAATGCTCCCCTTCTCGGTATATTGGCACGCCTAAATGCCGGCCATGGCGGGTATAACCAGCTCCTACCAGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

239

Amino Acids

26.08

Weight (kDa)

8.79

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 5 - 222 2.8e-61 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 328
AccI GTMKAC 1 cut(s) 270
AciI CCGC 2 cut(s) 214, 690
AcoI YGGCCR 2 cut(s) 261, 682
AcuI CTGAAG 1 cut(s) 290
AfaI GTAC 3 cut(s) 128, 146, 301
AfiI CCNNNNNNNGG 4 cut(s) 136, 361, 448, 656
AgeI ACCGGT 1 cut(s) 11
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 2 cut(s) 293, 709
AluBI AGCT 4 cut(s) 340, 495, 533, 703
AluI AGCT 4 cut(s) 340, 495, 533, 703
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 600
AlwNI CAGNNNCTG 1 cut(s) 629
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 3 cut(s) 202, 261, 682
ApeKI GCWGC 2 cut(s) 174, 495
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AsiGI ACCGGT 1 cut(s) 11
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 2 cut(s) 54, 198
AsuHPI GGTGA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 342
BarI GAAGNNNNNNTAC 2 cut(s) 128, 160
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 186, 482
BciT130I CCWGG 2 cut(s) 295, 711
BciVI GTATCC 1 cut(s) 60
BclI TGATCA 1 cut(s) 598
BcnI CCSGG 2 cut(s) 54, 198
BcoDI GTCTC 1 cut(s) 600
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 2 cut(s) 399, 534
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 1 cut(s) 561
BfuAI ACCTGC 1 cut(s) 89
BfuI GTATCC 1 cut(s) 60
BisI GCNGC 2 cut(s) 175, 496
BlsI GCNGC 2 cut(s) 176, 497
BmcAI AGTACT 1 cut(s) 146
Bme1390I CCNGG 4 cut(s) 54, 198, 295, 711
Bme18I GGWCC 1 cut(s) 572
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 10, 330
BmrFI CCNGG 4 cut(s) 54, 198, 295, 711
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 197
BpuMI CCSGG 2 cut(s) 54, 198
BsaJI CCNNGG 2 cut(s) 685, 710
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 136, 361, 448, 656
Bse118I RCCGGY 2 cut(s) 11, 680
Bse1I ACTGG 2 cut(s) 246, 559
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 2 cut(s) 295, 711
BseDI CCNNGG 2 cut(s) 685, 710
BseGI GGATG 1 cut(s) 46
BseLI CCNNNNNNNGG 4 cut(s) 136, 361, 448, 656
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 2 cut(s) 132, 374
BseNI ACTGG 2 cut(s) 246, 559
BseXI GCAGC 2 cut(s) 186, 482
BshFI GGCC 3 cut(s) 204, 263, 684
BshNI GGYRCC 1 cut(s) 328
BshTI ACCGGT 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 342
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 5 cut(s) 12, 54, 198, 526, 681
BslI CCNNNNNNNGG 4 cut(s) 136, 361, 448, 656
BsmAI GTCTC 1 cut(s) 600
BsnI GGCC 3 cut(s) 204, 263, 684
BsoBI CYCGRG 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 1 cut(s) 598
Bsp19I CCATGG 1 cut(s) 685
BspACI CCGC 2 cut(s) 214, 690
BspANI GGCC 3 cut(s) 204, 263, 684
BspCNI CTCAG 2 cut(s) 133, 373
BspLI GGNNCC 2 cut(s) 10, 330
BspMAI CTGCAG 1 cut(s) 500
BspMI ACCTGC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 2 cut(s) 11, 680
BsrI ACTGG 2 cut(s) 246, 559
BssAI RCCGGY 2 cut(s) 11, 680
BssECI CCNNGG 2 cut(s) 685, 710
BssMI GATC 1 cut(s) 598
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 2 cut(s) 295, 711
Bst4CI ACNGT 1 cut(s) 268
BstC8I GCNNGC 3 cut(s) 630, 670, 682
BstDEI CTNAG 2 cut(s) 141, 360
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 1 cut(s) 601
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 1 cut(s) 598
BstMWI GCNNNNNNNGC 2 cut(s) 504, 625
BstNI CCWGG 2 cut(s) 295, 711
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 4 cut(s) 52, 196, 293, 709
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 2 cut(s) 186, 482
BstV2I GAAGAC 1 cut(s) 197
BsuI GTATCC 1 cut(s) 60
BsuRI GGCC 3 cut(s) 204, 263, 684
BtgI CCRYGG 1 cut(s) 685
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 3 cut(s) 120, 253, 314
BveI ACCTGC 1 cut(s) 89
Cac8I GCNNGC 3 cut(s) 630, 670, 682
CaiI CAGNNNCTG 1 cut(s) 629
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 2 cut(s) 11, 680
Cfr13I GGNCC 1 cut(s) 572
Csp6I GTAC 3 cut(s) 127, 145, 300
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 6 cut(s) 22, 151, 159, 232, 406, 686
CviQI GTAC 3 cut(s) 127, 145, 300
DdeI CTNAG 2 cut(s) 141, 360
DpnI GATC 1 cut(s) 600
DpnII GATC 1 cut(s) 598
EaeI YGGCCR 2 cut(s) 261, 682
Ecl136II GAGCTC 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 685
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 572
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 388
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 293, 709
EcoT14I CCWWGG 1 cut(s) 685
EcoT38I GRGCYC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 685
FaeI CATG 6 cut(s) 25, 154, 162, 235, 409, 689
FatI CATG 6 cut(s) 21, 150, 158, 231, 405, 685
FauI CCCGC 1 cut(s) 683
FbaI TGATCA 1 cut(s) 598
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 175, 496
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 175, 496
FspBI CTAG 2 cut(s) 399, 534
GlaI GCGC 1 cut(s) 559
GluI GCNGC 2 cut(s) 175, 496
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 3 cut(s) 204, 263, 684
HapII CCGG 5 cut(s) 12, 54, 198, 526, 681
HhaI GCGC 1 cut(s) 560
Hin1II CATG 6 cut(s) 25, 154, 162, 235, 409, 689
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HinfI GANTC 2 cut(s) 193, 479
HpaII CCGG 5 cut(s) 12, 54, 198, 526, 681
HphI GGTGA 1 cut(s) 235
Hpy166II GTNNAC 3 cut(s) 271, 442, 572
Hpy188I TCNGA 3 cut(s) 123, 277, 603
Hpy8I GTNNAC 3 cut(s) 271, 442, 572
HpyAV CCTTC 4 cut(s) 130, 265, 469, 662
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 3 cut(s) 88, 235, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 504, 625
HpyF3I CTNAG 2 cut(s) 141, 360
Hsp92II CATG 6 cut(s) 25, 154, 162, 235, 409, 689
HspAI GCGC 1 cut(s) 558
KroI GCCGGC 1 cut(s) 680
KroNI GCCGGC 1 cut(s) 682
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 1 cut(s) 598
LmnI GCTCC 4 cut(s) 18, 345, 652, 708
Lsp1109I GCAGC 2 cut(s) 186, 482
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 399, 534
MaeIII GTNAC 3 cut(s) 241, 401, 536
MalI GATC 1 cut(s) 600
MboI GATC 1 cut(s) 598
MboII GAAGA 2 cut(s) 202, 282
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 2 cut(s) 187, 473
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 5 cut(s) 213, 262, 441, 466, 497
Mox20I TGGCCA 1 cut(s) 263
MroNI GCCGGC 1 cut(s) 680
MscI TGGCCA 1 cut(s) 263
MseI TTAA 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 5 cut(s) 12, 54, 198, 526, 681
MspR9I CCNGG 4 cut(s) 54, 198, 295, 711
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 2 cut(s) 295, 711
MwoI GCNNNNNNNGC 2 cut(s) 504, 625
NaeI GCCGGC 1 cut(s) 682
NciI CCSGG 2 cut(s) 54, 198
NcoI CCATGG 1 cut(s) 685
NdeII GATC 1 cut(s) 598
NgoMIV GCCGGC 1 cut(s) 680
NlaIII CATG 6 cut(s) 25, 154, 162, 235, 409, 689
NlaIV GGNNCC 2 cut(s) 10, 330
NmuCI GTSAC 2 cut(s) 241, 536
NspI RCATGY 1 cut(s) 235
PdiI GCCGGC 1 cut(s) 682
PfoI TCCNGGA 1 cut(s) 293
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 2 cut(s) 176, 497
PleI GAGTC 2 cut(s) 187, 473
PpsI GAGTC 2 cut(s) 187, 473
Psp124BI GAGCTC 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 293, 709
PspGI CCWGG 2 cut(s) 293, 709
PspN4I GGNNCC 2 cut(s) 10, 330
PspPI GGNCC 1 cut(s) 572
PstI CTGCAG 1 cut(s) 500
PstNI CAGNNNCTG 1 cut(s) 629
RsaI GTAC 3 cut(s) 128, 146, 301
RsaNI GTAC 3 cut(s) 127, 145, 300
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 2 cut(s) 175, 496
Sau3AI GATC 1 cut(s) 598
Sau96I GGNCC 1 cut(s) 572
ScaI AGTACT 1 cut(s) 146
SchI GAGTC 2 cut(s) 187, 473
ScrFI CCNGG 4 cut(s) 54, 198, 295, 711
SduI GDGCHC 1 cut(s) 342
SetI ASST 9 cut(s) 103, 224, 342, 366, 497, 535, 577, 638, 705
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 1 cut(s) 572
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 1 cut(s) 459
SsiI CCGC 2 cut(s) 214, 690
SspMI CTAG 2 cut(s) 399, 534
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 4 cut(s) 52, 196, 293, 709
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 1 cut(s) 268
TaqII GACCGA 1 cut(s) 41
TasI AATT 1 cut(s) 459
TatI WGTACW 1 cut(s) 144
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 3 cut(s) 120, 253, 314
TseFI GTSAC 2 cut(s) 241, 536
TseI GCWGC 2 cut(s) 174, 495
Tsp45I GTSAC 2 cut(s) 241, 536
TspDTI ATGAA 2 cut(s) 139, 147
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 3 cut(s) 120, 253, 314
VpaK11BI GGWCC 1 cut(s) 572
XceI RCATGY 1 cut(s) 235
XcmI CCANNNNNNNNNTGG 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 270
XspI CTAG 2 cut(s) 399, 534
ZrmI AGTACT 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.