Rmu_co8419631.1_g000001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8419631.1
OG0008244
Physical Location & Seq
Forward (+)
1 .. 842
842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8419631.1_g000001.1.cds

Sequence Viewer

Length: 705 bp
gtggactacattctcggagacaacccaagagccactagctacatggttggatatggaaacaactatcctcaacaagttcaccacagggcttcctcaattgtttccatcaagaaggattcttcatttgtgagctgcaggggaggttatgctacttggtttagcaggaaggctagtgaccctaatctccttacgggtgctattgttggtggacctgatgcctatgacaactttgctgatcagagggacaactatgagcagaccgaacctgctacctacaacaatgctcctcttatcggtttattggctcgtctaggaggcggtcagagcggctataaccagcttcttccagttgttacaacccagccaaaacaagctccactacctaaggttactccagctgctccagcttcatcttctggcccaattgcaatagcacagaaggtgacatcttcatgggttgccaagggagtaacttactacagatattccacaactgtgaccaacaagtctggcaagacactcaaaaacctcaagcttacaatatctaaactgtacggtcctctttggggtcttacaaagaccactgattcctatgtttttccaagctggctcaactctttacctgcgggaaagagcctcgagtttgtctatatccattctgcttctgcagcaaatgtcttggtctcaagctactcattagcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

234

Amino Acids

25.16

Weight (kDa)

9.5

Isoelectric Point (pI)

30.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 505
Acc36I ACCTGC 2 cut(s) 274, 631
AccBSI CCGCTC 1 cut(s) 327
AciI CCGC 3 cut(s) 318, 327, 626
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 2 cut(s) 293, 566
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 9 cut(s) 39, 132, 340, 374, 398, 407, 535, 606, 690
AluI AGCT 9 cut(s) 39, 132, 340, 374, 398, 407, 535, 606, 690
Alw26I GTCTC 2 cut(s) 12, 688
Ama87I CYCGRG 1 cut(s) 638
AoxI GGCC 1 cut(s) 418
ApeKI GCWGC 3 cut(s) 132, 398, 668
AspS9I GGNCC 3 cut(s) 209, 419, 557
AsuHPI GGTGA 2 cut(s) 71, 454
AvaI CYCGRG 1 cut(s) 638
AvaII GGWCC 2 cut(s) 209, 557
AxyI CCTNAGG 1 cut(s) 384
BbvI GCAGC 3 cut(s) 119, 385, 680
BccI CCATC 1 cut(s) 113
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 12, 688
BfaI CTAG 3 cut(s) 36, 171, 311
BfmI CTRYAG 3 cut(s) 133, 478, 666
BfuAI ACCTGC 2 cut(s) 274, 631
BisI GCNGC 4 cut(s) 133, 328, 399, 669
BlsI GCNGC 4 cut(s) 134, 329, 400, 670
Bme18I GGWCC 2 cut(s) 209, 557
BmeT110I CYCGRG 1 cut(s) 638
BmgT120I GGNCC 3 cut(s) 209, 419, 557
BmsI GCATC 1 cut(s) 205
BpmI CTGGAG 2 cut(s) 378, 387
BpuEI CTTGAG 2 cut(s) 515, 670
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 1 cut(s) 462
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 566
Bse1I ACTGG 1 cut(s) 347
Bse21I CCTNAGG 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 462
BseLI CCNNNNNNNGG 2 cut(s) 293, 566
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 3 cut(s) 119, 385, 680
BseYI CCCAGC 1 cut(s) 360
BshFI GGCC 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 638
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 293, 566
BsmAI GTCTC 2 cut(s) 12, 688
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 1 cut(s) 420
Bso31I GGTCTC 1 cut(s) 688
BsoBI CYCGRG 1 cut(s) 638
Bsp143I GATC 1 cut(s) 235
BspACI CCGC 3 cut(s) 318, 327, 626
BspANI GGCC 1 cut(s) 420
BspMAI CTGCAG 2 cut(s) 137, 670
BspMI ACCTGC 2 cut(s) 274, 631
BspTNI GGTCTC 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 327
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 1 cut(s) 235
BssT1I CCWWGG 1 cut(s) 462
Bst4CI ACNGT 3 cut(s) 496, 552, 557
BstC8I GCNNGC 1 cut(s) 608
BstDEI CTNAG 1 cut(s) 384
BstKTI GATC 1 cut(s) 238
BstMAI GTCTC 2 cut(s) 12, 688
BstMBI GATC 1 cut(s) 235
BstMWI GCNNNNNNNGC 3 cut(s) 324, 404, 668
BstSFI CTRYAG 3 cut(s) 133, 478, 666
BstV1I GCAGC 3 cut(s) 119, 385, 680
Bsu36I CCTNAGG 1 cut(s) 384
BsuRI GGCC 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 582
BveI ACCTGC 2 cut(s) 274, 631
Cac8I GCNNGC 1 cut(s) 608
Cfr13I GGNCC 3 cut(s) 209, 419, 557
Csp6I GTAC 1 cut(s) 553
CviAII CATG 2 cut(s) 43, 453
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 1 cut(s) 384
DpnI GATC 1 cut(s) 237
DpnII GATC 1 cut(s) 235
DrdI GACNNNNNNGTC 1 cut(s) 505
DseDI GACNNNNNNGTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 462
Eco31I GGTCTC 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 209, 557
Eco81I CCTNAGG 1 cut(s) 384
Eco88I CYCGRG 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 2 cut(s) 46, 456
FaiI YATR 9 cut(s) 44, 54, 147, 222, 252, 333, 454, 594, 651
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 42, 452
FauI CCCGC 1 cut(s) 619
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 4 cut(s) 133, 328, 399, 669
Fsp4HI GCNGC 4 cut(s) 133, 328, 399, 669
FspBI CTAG 3 cut(s) 36, 171, 311
GluI GCNGC 4 cut(s) 133, 328, 399, 669
GsaI CCCAGC 1 cut(s) 364
GsuI CTGGAG 2 cut(s) 378, 387
HaeIII GGCC 1 cut(s) 420
Hin1II CATG 2 cut(s) 46, 456
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 2 cut(s) 116, 587
HphI GGTGA 2 cut(s) 71, 454
Hpy166II GTNNAC 3 cut(s) 4, 79, 209
Hpy188I TCNGA 3 cut(s) 17, 240, 324
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 3 cut(s) 4, 79, 209
HpyAV CCTTC 3 cut(s) 106, 160, 433
HpyCH4III ACNGT 3 cut(s) 496, 552, 557
HpyCH4V TGCA 3 cut(s) 135, 428, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 324, 404, 668
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 2 cut(s) 46, 456
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 1 cut(s) 235
LmnI GCTCC 3 cut(s) 289, 379, 406
Lsp1109I GCAGC 3 cut(s) 119, 385, 680
LweI GCATC 1 cut(s) 205
MaeI CTAG 3 cut(s) 36, 171, 311
MaeIII GTNAC 6 cut(s) 173, 352, 388, 442, 469, 496
MalI GATC 1 cut(s) 237
MbiI CCGCTC 1 cut(s) 327
MboI GATC 1 cut(s) 235
MboII GAAGA 4 cut(s) 111, 335, 405, 441
MfeI CAATTG 2 cut(s) 96, 423
MluCI AATT 2 cut(s) 96, 423
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 9 cut(s) 78, 103, 134, 234, 297, 308, 539, 570, 647
MslI CAYNNNNRTG 3 cut(s) 451, 494, 700
MspA1I CMGCKG 1 cut(s) 398
MunI CAATTG 2 cut(s) 96, 423
MwoI GCNNNNNNNGC 3 cut(s) 324, 404, 668
NdeII GATC 1 cut(s) 235
NlaIII CATG 2 cut(s) 46, 456
NmuCI GTSAC 3 cut(s) 173, 442, 496
OliI CACNNNNGTG 1 cut(s) 494
PaeR7I CTCGAG 1 cut(s) 638
PfeI GAWTC 2 cut(s) 116, 587
PkrI GCNGC 4 cut(s) 134, 329, 400, 670
PspFI CCCAGC 1 cut(s) 360
PspPI GGNCC 3 cut(s) 209, 419, 557
PspXI VCTCGAGB 1 cut(s) 638
PstI CTGCAG 2 cut(s) 137, 670
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 3 cut(s) 451, 494, 700
SatI GCNGC 4 cut(s) 133, 328, 399, 669
Sau3AI GATC 1 cut(s) 235
Sau96I GGNCC 3 cut(s) 209, 419, 557
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 3 cut(s) 133, 478, 666
Sfr274I CTCGAG 1 cut(s) 638
SinI GGWCC 2 cut(s) 209, 557
SlaI CTCGAG 1 cut(s) 638
SmiMI CAYNNNNRTG 3 cut(s) 451, 494, 700
SmlI CTYRAG 3 cut(s) 530, 638, 685
SmoI CTYRAG 3 cut(s) 530, 638, 685
Sse9I AATT 2 cut(s) 96, 423
SsiI CCGC 3 cut(s) 318, 327, 626
SspMI CTAG 3 cut(s) 36, 171, 311
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 3 cut(s) 496, 552, 557
TaqI TCGA 1 cut(s) 639
TaqII GACCGA 1 cut(s) 275
TasI AATT 2 cut(s) 96, 423
TauI GCSGC 1 cut(s) 330
TfiI GAWTC 2 cut(s) 116, 587
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 3 cut(s) 173, 442, 496
TseI GCWGC 3 cut(s) 132, 398, 668
Tsp45I GTSAC 3 cut(s) 173, 442, 496
TspDTI ATGAA 3 cut(s) 111, 399, 441
TspRI CASTG 1 cut(s) 589
VpaK11BI GGWCC 2 cut(s) 209, 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XhoI CTCGAG 1 cut(s) 638
XspI CTAG 3 cut(s) 36, 171, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.