Rmu_co8181580.1_g000001

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8181580.1
OG0008244
Physical Location & Seq
Forward (+)
61 .. 686
626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8181580.1_g000001.1.cds

Sequence Viewer

Length: 377 bp
atggcgttcactgttactatgatgtcctggagtataatagagtacggaaagcaaatggcttcaagcggtgaacttgggcatgccatggacgctgtcaagtggggcactgactacttcattaaagctcacccagaacccaacgttctctatggagaggtcggagacgggaacagtgaccactactgctggcagaggccggaggacatgaccaccgaccgccacgcttacaagatcagcccgagtaaccccgggtcggatctcgccggagaaaccgccgccgccatggccgccgcctccattgtgttccaccgcaccaaccccgcctactcccgcgagctcttacagcatgcgtaccaggttcgtctcactccctccgc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

126

Amino Acids

13.91

Weight (kDa)

5.57

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 333
AclI AACGTT 1 cut(s) 141
AclWI GGATC 1 cut(s) 264
AcoI YGGCCR 1 cut(s) 285
AfaI GTAC 2 cut(s) 44, 353
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 1 cut(s) 63
AjnI CCWGG 2 cut(s) 26, 354
AluBI AGCT 2 cut(s) 125, 337
AluI AGCT 2 cut(s) 125, 337
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 156, 368
AlwI GGATC 1 cut(s) 264
Ama87I CYCGRG 2 cut(s) 238, 248
AoxI GGCC 2 cut(s) 194, 285
AsuC2I CCSGG 2 cut(s) 249, 250
AsuHPI GGTGA 2 cut(s) 80, 119
AvaI CYCGRG 2 cut(s) 238, 248
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BciT130I CCWGG 2 cut(s) 28, 356
BcnI CCSGG 2 cut(s) 249, 250
BcoDI GTCTC 2 cut(s) 156, 368
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 4 cut(s) 276, 279, 288, 291
BlsI GCNGC 4 cut(s) 277, 280, 289, 292
Bme1390I CCNGG 4 cut(s) 28, 249, 250, 356
BmeT110I CYCGRG 2 cut(s) 238, 248
BmrFI CCNGG 4 cut(s) 28, 249, 250, 356
BpmI CTGGAG 1 cut(s) 49
BpuMI CCSGG 2 cut(s) 249, 250
BsaJI CCNNGG 4 cut(s) 84, 247, 248, 282
Bsc4I CCNNNNNNNGG 1 cut(s) 253
BseBI CCWGG 2 cut(s) 28, 356
BseDI CCNNGG 4 cut(s) 84, 247, 248, 282
BseLI CCNNNNNNNGG 1 cut(s) 253
BseSI GKGCMC 1 cut(s) 107
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 2 cut(s) 196, 287
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 2 cut(s) 238, 248
BsiSI CCGG 3 cut(s) 197, 249, 264
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 2 cut(s) 156, 368
BsmBI CGTCTC 2 cut(s) 156, 368
BsnI GGCC 2 cut(s) 196, 287
BsoBI CYCGRG 2 cut(s) 238, 248
Bsp1286I GDGCHC 2 cut(s) 107, 339
Bsp143I GATC 2 cut(s) 231, 256
Bsp19I CCATGG 2 cut(s) 84, 282
BspANI GGCC 2 cut(s) 196, 287
BspFNI CGCG 1 cut(s) 333
BspPI GGATC 1 cut(s) 264
BssECI CCNNGG 4 cut(s) 84, 247, 248, 282
BssMI GATC 2 cut(s) 231, 256
BssT1I CCWWGG 2 cut(s) 84, 282
Bst2UI CCWGG 2 cut(s) 28, 356
Bst4CI ACNGT 2 cut(s) 13, 173
BstC8I GCNNGC 4 cut(s) 81, 188, 335, 348
BstDSI CCRYGG 2 cut(s) 84, 282
BstFNI CGCG 1 cut(s) 333
BstKTI GATC 2 cut(s) 234, 259
BstMAI GTCTC 2 cut(s) 156, 368
BstMBI GATC 2 cut(s) 231, 256
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 89, 284, 287, 343
BstNI CCWGG 2 cut(s) 28, 356
BstNSI RCATGY 2 cut(s) 83, 350
BstSCI CCNGG 4 cut(s) 26, 247, 248, 354
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 256
BstYI RGATCY 1 cut(s) 256
BsuRI GGCC 2 cut(s) 196, 287
BtgI CCRYGG 2 cut(s) 84, 282
BtsIMutI CAGTG 3 cut(s) 9, 105, 178
Cac8I GCNNGC 4 cut(s) 81, 188, 335, 348
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 1 cut(s) 98
CsiI ACCWGGT 1 cut(s) 354
Csp6I GTAC 2 cut(s) 43, 352
CviAII CATG 5 cut(s) 80, 85, 205, 283, 347
CviJI RGCY 6 cut(s) 59, 125, 196, 237, 287, 337
CviKI_1 RGCY 6 cut(s) 59, 125, 196, 237, 287, 337
CviQI GTAC 2 cut(s) 43, 352
DpnI GATC 2 cut(s) 233, 258
DpnII GATC 2 cut(s) 231, 256
EaeI YGGCCR 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 84, 282
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 2 cut(s) 238, 248
EcoICRI GAGCTC 1 cut(s) 337
EcoRII CCWGG 2 cut(s) 26, 354
EcoT14I CCWWGG 2 cut(s) 84, 282
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 2 cut(s) 84, 282
Esp3I CGTCTC 2 cut(s) 156, 368
FaeI CATG 5 cut(s) 83, 88, 208, 286, 350
FaiI YATR 8 cut(s) 20, 35, 81, 86, 150, 206, 284, 348
FatI CATG 5 cut(s) 79, 84, 204, 282, 346
FauI CCCGC 2 cut(s) 328, 338
Fnu4HI GCNGC 4 cut(s) 276, 279, 288, 291
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 4 cut(s) 276, 279, 288, 291
GluI GCNGC 4 cut(s) 276, 279, 288, 291
GsuI CTGGAG 1 cut(s) 49
HaeIII GGCC 2 cut(s) 196, 287
HapII CCGG 3 cut(s) 197, 249, 264
HgaI GACGC 1 cut(s) 98
Hin1II CATG 5 cut(s) 83, 88, 208, 286, 350
HpaII CCGG 3 cut(s) 197, 249, 264
HphI GGTGA 2 cut(s) 80, 119
Hpy166II GTNNAC 2 cut(s) 9, 71
Hpy188I TCNGA 2 cut(s) 161, 256
Hpy8I GTNNAC 2 cut(s) 9, 71
HpyCH4III ACNGT 2 cut(s) 13, 173
HpyCH4IV ACGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 284, 287, 343
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 5 cut(s) 83, 88, 208, 286, 350
Kzo9I GATC 2 cut(s) 231, 256
LpnPI CCDG 9 cut(s) 13, 40, 144, 172, 210, 262, 277, 341, 368
MabI ACCWGGT 1 cut(s) 354
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 13, 173, 242
MalI GATC 2 cut(s) 233, 258
MboI GATC 2 cut(s) 231, 256
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 2 cut(s) 107, 339
MmeI TCCRAC 2 cut(s) 139, 234
MnlI CCTC 4 cut(s) 148, 186, 193, 304
MseI TTAA 1 cut(s) 120
MspI CCGG 3 cut(s) 197, 249, 264
MspR9I CCNGG 4 cut(s) 28, 249, 250, 356
MvaI CCWGG 2 cut(s) 28, 356
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 89, 284, 287, 343
NciI CCSGG 2 cut(s) 249, 250
NcoI CCATGG 2 cut(s) 84, 282
NdeII GATC 2 cut(s) 231, 256
NlaIII CATG 5 cut(s) 83, 88, 208, 286, 350
NmuCI GTSAC 1 cut(s) 173
NspI RCATGY 2 cut(s) 83, 350
PaeI GCATGC 2 cut(s) 83, 350
PflFI GACNNNGTC 1 cut(s) 92
PfoI TCCNGGA 1 cut(s) 26
PkrI GCNGC 4 cut(s) 277, 280, 289, 292
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 26, 354
PspGI CCWGG 2 cut(s) 26, 354
PsuI RGATCY 1 cut(s) 256
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 2 cut(s) 44, 353
RsaNI GTAC 2 cut(s) 43, 352
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 4 cut(s) 276, 279, 288, 291
Sau3AI GATC 2 cut(s) 231, 256
ScrFI CCNGG 4 cut(s) 28, 249, 250, 356
SduI GDGCHC 2 cut(s) 107, 339
SetI ASST 5 cut(s) 127, 144, 159, 339, 360
SexAI ACCWGGT 1 cut(s) 354
SmaI CCCGGG 1 cut(s) 250
SphI GCATGC 2 cut(s) 83, 350
SstI GAGCTC 1 cut(s) 339
StyD4I CCNGG 4 cut(s) 26, 247, 248, 354
StyI CCWWGG 2 cut(s) 84, 282
TaaI ACNGT 2 cut(s) 13, 173
TaiI ACGT 1 cut(s) 144
TauI GCSGC 4 cut(s) 278, 281, 290, 293
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 3 cut(s) 16, 112, 178
TseFI GTSAC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 106
TspGWI ACGGA 1 cut(s) 60
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 3 cut(s) 16, 112, 178
Tth111I GACNNNGTC 1 cut(s) 92
XceI RCATGY 2 cut(s) 83, 350
XmaI CCCGGG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.