Rh7AG147400

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008244
Physical Location & Seq
Reverse (-)
11951713 .. 11955839
4127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG147400.1

Sequence Viewer

Length: 1398 bp
ATGACCACCGACCGCCACGCTTACAAGATCAGCCCGAGTAACCCCGGGTCGGATCTCGCCGGAGAAACCGCCGCCGCCATGGCCGCCGCCTCCATTGTGTTCCACCGCACCAACCCCGCCTACTCCCGCGAGCTCTTACAGCATGCGTACGAGCTGTTTGATTTTGCAGACAAGTACAGGGGCAAATATGACAGCAGCATTACAGTTGCTCAAAAGTACTACCGATCCATCAGTGGCTACAATGATGAGTTGCTTTGGGCCGCTGCTTGGTTGTACCAGGCTTCCAACAATGAGTACTACTTGAACTACCTTGCTAACAATGGTGACTCCATGGGTGGAACTGGTTGGGGCATGACCGAGTTTGGTTGGGATGTCAAGTACTCTGGTGTTCAGACCCTTGTTGCTAAGTTCTTAATGCAAGGCAAAGCTGGTAAGCATGCTGCTGTCTTTGAGAAGTACACGGCGAAAGCTGAGTACTTTATGTGTTCATGCCTCGGAAAGGGCAGCCGCAATGTGCAGAAGACTCCTGGTGGCCTCATTTTCCGTCAAAGGTGGAACAACATGCAATTTGTGACAAGTGCCTCCTTCTTGGCCACAGTCTACTCCGACTATCTTACATCTTCCAGGAGAACACTGAAGTGTGCTTCTGGCAATGTGGCACCTACTGAGCTTTTATCCTTTGCAAAATCTCAGGTGGACTACATTCTCGGAGACAACCCAAGAGCCACTAGCTACATGGTTGGATATGGAAACAACTATCCTCAACAAGTTCACCACAGGGCTTCCTCAATTGTTTCCATCAAGAAGGATTCTTCATTTGTGAGCTGCAGGGGAGGTTATGCTACTTGGTTTAGCAGGAAGGCTAGTGACCCTAATCTCCTTACGGGTGCTATTGTTGGTGGACCTGATGCCTATGACAACTTTGCTGATCAGAGGGACAACTATGAGCAGACCGAACCTGCTACCTACAACAATGCTCCTCTTATCGGTTTATTGGCTCGTCTAGGAGGCGGTCAGAGCGGCTATAACCAGCTTCTTCCAGTTGTTACAACCCAGCCAAAACAAGCTCCACTACCTAAGGTTACTCCAGCTGCTCCAGCTTCATCTTCTGGCCCAATTGCAATAGCACAGAAGGTGACATCTTCATGGGTTGCCAAGGGAGTAACTTACTACAGATATTCCACAACTGTGACCAACAAGTCTGGCAAGACACTCAAAAACCTCAAGCTTACAATATCTAAACTGTACGGTCCTCTTTGGGGTCTTACAAAGACCACTGATTCCTATGTTTTTCCAAGCTGGCTCAACTCTTTACCTGCGGGAAAGAGCCTCGAGTTTGTCTACATCCATTCTGCTTCTGCAGCAAATGTCTTGGTCTCAAGCTACTCATTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

465

Amino Acids

50.92

Weight (kDa)

9.31

Isoelectric Point (pI)

30.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 1 - 332 1.9e-103 Glycosyl hydrolase family 9
CBM49 PF09478 373 - 450 1.8e-28 Carbohydrate binding domain CBM49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1198
Acc36I ACCTGC 2 cut(s) 967, 1324
AccB1I GGYRCC 1 cut(s) 658
AccBSI CCGCTC 1 cut(s) 1020
AccI GTMKAC 2 cut(s) 600, 1341
AccII CGCG 1 cut(s) 129
AclWI GGATC 2 cut(s) 60, 219
AcoI YGGCCR 2 cut(s) 81, 591
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 9 cut(s) 149, 176, 218, 275, 296, 380, 458, 476, 1247
AfiI CCNNNNNNNGG 5 cut(s) 49, 267, 499, 986, 1259
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 3 cut(s) 276, 526, 623
AleI CACNNNNGTG 2 cut(s) 637, 1187
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 705, 1381
AlwI GGATC 2 cut(s) 60, 219
Ama87I CYCGRG 3 cut(s) 34, 44, 1331
AoxI GGCC 5 cut(s) 81, 258, 532, 591, 1111
ApeKI GCWGC 7 cut(s) 195, 263, 440, 504, 825, 1091, 1361
AspS9I GGNCC 4 cut(s) 258, 902, 1112, 1250
AsuC2I CCSGG 2 cut(s) 45, 46
AsuHPI GGTGA 3 cut(s) 335, 764, 1147
AvaI CYCGRG 3 cut(s) 34, 44, 1331
AvaII GGWCC 2 cut(s) 902, 1250
AxyI CCTNAGG 1 cut(s) 1077
BalI TGGCCA 1 cut(s) 593
BanI GGYRCC 1 cut(s) 658
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 7 cut(s) 207, 250, 427, 516, 812, 1078, 1373
BccI CCATC 2 cut(s) 236, 806
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 3 cut(s) 278, 528, 625
BclI TGATCA 1 cut(s) 928
BcnI CCSGG 2 cut(s) 45, 46
BcoDI GTCTC 2 cut(s) 705, 1381
BfaI CTAG 3 cut(s) 729, 864, 1004
BfmI CTRYAG 3 cut(s) 826, 1171, 1359
BfuAI ACCTGC 2 cut(s) 967, 1324
BglI GCCNNNNNGGC 1 cut(s) 80
BmcAI AGTACT 4 cut(s) 218, 296, 380, 476
Bme1390I CCNGG 5 cut(s) 45, 46, 278, 528, 625
Bme18I GGWCC 2 cut(s) 902, 1250
BmeT110I CYCGRG 3 cut(s) 34, 44, 1331
BmgT120I GGNCC 4 cut(s) 258, 902, 1112, 1250
BmiI GGNNCC 1 cut(s) 660
BmrFI CCNGG 5 cut(s) 45, 46, 278, 528, 625
BmsI GCATC 1 cut(s) 898
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 2 cut(s) 1071, 1080
BpuEI CTTGAG 2 cut(s) 1208, 1363
BpuMI CCSGG 2 cut(s) 45, 46
BsaI GGTCTC 1 cut(s) 1381
BsaJI CCNNGG 6 cut(s) 43, 44, 78, 330, 493, 1155
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 267, 499, 986, 1259
Bse1I ACTGG 2 cut(s) 346, 1040
Bse21I CCTNAGG 1 cut(s) 1077
Bse3DI GCAATG 2 cut(s) 517, 658
BseBI CCWGG 3 cut(s) 278, 528, 625
BseDI CCNNGG 6 cut(s) 43, 44, 78, 330, 493, 1155
BseGI GGATG 2 cut(s) 376, 1344
BseLI CCNNNNNNNGG 5 cut(s) 49, 267, 499, 986, 1259
BseMI GCAATG 2 cut(s) 517, 658
BseMII CTCAG 3 cut(s) 462, 657, 704
BseNI ACTGG 2 cut(s) 346, 1040
BseRI GAGGAG 1 cut(s) 969
BseXI GCAGC 7 cut(s) 207, 250, 427, 516, 812, 1078, 1373
BseYI CCCAGC 1 cut(s) 1053
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 129
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 5 cut(s) 83, 260, 534, 593, 1113
BshNI GGYRCC 1 cut(s) 658
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 3 cut(s) 34, 44, 1331
BsiSI CCGG 2 cut(s) 45, 60
BsiWI CGTACG 1 cut(s) 147
BslFI GGGAC 1 cut(s) 950
BslI CCNNNNNNNGG 5 cut(s) 49, 267, 499, 986, 1259
BsmAI GTCTC 2 cut(s) 705, 1381
BsmFI GGGAC 1 cut(s) 950
BsnI GGCC 5 cut(s) 83, 260, 534, 593, 1113
Bso31I GGTCTC 1 cut(s) 1381
BsoBI CYCGRG 3 cut(s) 34, 44, 1331
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 4 cut(s) 27, 52, 224, 928
Bsp19I CCATGG 2 cut(s) 78, 330
BspANI GGCC 5 cut(s) 83, 260, 534, 593, 1113
BspCNI CTCAG 3 cut(s) 463, 658, 703
BspFNI CGCG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 660
BspMAI CTGCAG 2 cut(s) 830, 1363
BspMI ACCTGC 2 cut(s) 967, 1324
BspPI GGATC 2 cut(s) 60, 219
BspT107I GGYRCC 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 1381
BsrBI CCGCTC 1 cut(s) 1020
BsrDI GCAATG 2 cut(s) 517, 658
BsrI ACTGG 2 cut(s) 346, 1040
BssECI CCNNGG 6 cut(s) 43, 44, 78, 330, 493, 1155
BssMI GATC 4 cut(s) 27, 52, 224, 928
BssT1I CCWWGG 3 cut(s) 78, 330, 1155
Bst2UI CCWGG 3 cut(s) 278, 528, 625
Bst4CI ACNGT 5 cut(s) 205, 598, 1189, 1245, 1250
BstC8I GCNNGC 4 cut(s) 131, 144, 438, 1301
BstDEI CTNAG 5 cut(s) 405, 471, 666, 690, 1077
BstDSI CCRYGG 2 cut(s) 78, 330
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 2 cut(s) 376, 1344
BstFNI CGCG 1 cut(s) 129
BstKTI GATC 4 cut(s) 30, 55, 227, 931
BstMAI GTCTC 2 cut(s) 705, 1381
BstMBI GATC 4 cut(s) 27, 52, 224, 928
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 6 cut(s) 80, 83, 139, 1017, 1097, 1361
BstNI CCWGG 3 cut(s) 278, 528, 625
BstNSI RCATGY 3 cut(s) 146, 440, 565
BstSCI CCNGG 5 cut(s) 43, 44, 276, 526, 623
BstSFI CTRYAG 3 cut(s) 826, 1171, 1359
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 7 cut(s) 207, 250, 427, 516, 812, 1078, 1373
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu36I CCTNAGG 1 cut(s) 1077
BsuRI GGCC 5 cut(s) 83, 260, 534, 593, 1113
BtgI CCRYGG 2 cut(s) 78, 330
BtsCI GGATG 2 cut(s) 376, 1344
BtsIMutI CAGTG 3 cut(s) 238, 632, 1275
BveI ACCTGC 2 cut(s) 967, 1324
Cac8I GCNNGC 4 cut(s) 131, 144, 438, 1301
Cfr13I GGNCC 4 cut(s) 258, 902, 1112, 1250
Cfr9I CCCGGG 1 cut(s) 44
Csp6I GTAC 9 cut(s) 148, 175, 217, 274, 295, 379, 457, 475, 1246
CviAII CATG 9 cut(s) 79, 143, 331, 352, 437, 489, 562, 736, 1146
CviQI GTAC 9 cut(s) 148, 175, 217, 274, 295, 379, 457, 475, 1246
DdeI CTNAG 5 cut(s) 405, 471, 666, 690, 1077
DpnI GATC 4 cut(s) 29, 54, 226, 930
DpnII GATC 4 cut(s) 27, 52, 224, 928
DrdI GACNNNNNNGTC 1 cut(s) 1198
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 2 cut(s) 81, 591
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 78, 330, 1155
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 1381
Eco47I GGWCC 2 cut(s) 902, 1250
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 656
Eco81I CCTNAGG 1 cut(s) 1077
Eco88I CYCGRG 3 cut(s) 34, 44, 1331
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoRII CCWGG 3 cut(s) 276, 526, 623
EcoT14I CCWWGG 3 cut(s) 78, 330, 1155
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 3 cut(s) 78, 330, 1155
FaeI CATG 9 cut(s) 82, 146, 334, 355, 440, 492, 565, 739, 1149
FaqI GGGAC 1 cut(s) 950
FatI CATG 9 cut(s) 78, 142, 330, 351, 436, 488, 561, 735, 1145
FauI CCCGC 3 cut(s) 124, 134, 1312
FbaI TGATCA 1 cut(s) 928
FblI GTMKAC 2 cut(s) 600, 1341
FokI GGATG 2 cut(s) 383, 1331
FriOI GRGCYC 1 cut(s) 135
FspBI CTAG 3 cut(s) 729, 864, 1004
GsaI CCCAGC 1 cut(s) 1057
GsuI CTGGAG 2 cut(s) 1071, 1080
HaeIII GGCC 5 cut(s) 83, 260, 534, 593, 1113
HapII CCGG 2 cut(s) 45, 60
Hin1II CATG 9 cut(s) 82, 146, 334, 355, 440, 492, 565, 739, 1149
HindIII AAGCTT 1 cut(s) 1226
HinfI GANTC 4 cut(s) 326, 523, 809, 1280
HpaII CCGG 2 cut(s) 45, 60
HphI GGTGA 3 cut(s) 335, 764, 1147
Hpy166II GTNNAC 6 cut(s) 459, 601, 697, 772, 902, 1342
Hpy188I TCNGA 7 cut(s) 52, 393, 497, 607, 710, 933, 1017
Hpy188III TCNNGA 1 cut(s) 802
Hpy8I GTNNAC 6 cut(s) 459, 601, 697, 772, 902, 1342
HpyAV CCTTC 4 cut(s) 595, 799, 853, 1126
HpyCH4III ACNGT 5 cut(s) 205, 598, 1189, 1245, 1250
HpyCH4V TGCA 8 cut(s) 167, 418, 517, 565, 683, 828, 1121, 1361
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 83, 139, 1017, 1097, 1361
HpyF3I CTNAG 5 cut(s) 405, 471, 666, 690, 1077
Hsp92II CATG 9 cut(s) 82, 146, 334, 355, 440, 492, 565, 739, 1149
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 4 cut(s) 27, 52, 224, 928
LmnI GCTCC 3 cut(s) 982, 1072, 1099
Lsp1109I GCAGC 7 cut(s) 207, 250, 427, 516, 812, 1078, 1373
LweI GCATC 1 cut(s) 898
MaeI CTAG 3 cut(s) 729, 864, 1004
MaeIII GTNAC 9 cut(s) 38, 323, 571, 866, 1045, 1081, 1135, 1162, 1189
MalI GATC 4 cut(s) 29, 54, 226, 930
MbiI CCGCTC 1 cut(s) 1020
MboI GATC 4 cut(s) 27, 52, 224, 928
MboII GAAGA 6 cut(s) 532, 612, 804, 1028, 1098, 1134
MfeI CAATTG 2 cut(s) 789, 1116
MflI RGATCY 1 cut(s) 52
MhlI GDGCHC 1 cut(s) 135
MlsI TGGCCA 1 cut(s) 593
MluCI AATT 3 cut(s) 566, 789, 1116
MluNI TGGCCA 1 cut(s) 593
MlyI GAGTC 2 cut(s) 320, 517
MmeI TCCRAC 4 cut(s) 30, 309, 630, 721
Mox20I TGGCCA 1 cut(s) 593
MscI TGGCCA 1 cut(s) 593
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 4 cut(s) 637, 1144, 1187, 1393
Msp20I TGGCCA 1 cut(s) 593
MspA1I CMGCKG 2 cut(s) 263, 1091
MspI CCGG 2 cut(s) 45, 60
MspR9I CCNGG 5 cut(s) 45, 46, 278, 528, 625
MunI CAATTG 2 cut(s) 789, 1116
MvaI CCWGG 3 cut(s) 278, 528, 625
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 6 cut(s) 80, 83, 139, 1017, 1097, 1361
NciI CCSGG 2 cut(s) 45, 46
NcoI CCATGG 2 cut(s) 78, 330
NdeII GATC 4 cut(s) 27, 52, 224, 928
NlaIII CATG 9 cut(s) 82, 146, 334, 355, 440, 492, 565, 739, 1149
NlaIV GGNNCC 1 cut(s) 660
NmuCI GTSAC 5 cut(s) 323, 571, 866, 1135, 1189
NspI RCATGY 3 cut(s) 146, 440, 565
OliI CACNNNNGTG 2 cut(s) 637, 1187
PaeI GCATGC 2 cut(s) 146, 440
PaeR7I CTCGAG 1 cut(s) 1331
PfeI GAWTC 2 cut(s) 809, 1280
Pfl23II CGTACG 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 623
PleI GAGTC 2 cut(s) 320, 517
PpsI GAGTC 2 cut(s) 320, 517
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 3 cut(s) 276, 526, 623
PspFI CCCAGC 1 cut(s) 1053
PspGI CCWGG 3 cut(s) 276, 526, 623
PspLI CGTACG 1 cut(s) 147
PspN4I GGNNCC 1 cut(s) 660
PspPI GGNCC 4 cut(s) 258, 902, 1112, 1250
PspXI VCTCGAGB 1 cut(s) 1331
PstI CTGCAG 2 cut(s) 830, 1363
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 1091
RsaI GTAC 9 cut(s) 149, 176, 218, 275, 296, 380, 458, 476, 1247
RsaNI GTAC 9 cut(s) 148, 175, 217, 274, 295, 379, 457, 475, 1246
RseI CAYNNNNRTG 4 cut(s) 637, 1144, 1187, 1393
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 413
Sau3AI GATC 4 cut(s) 27, 52, 224, 928
Sau96I GGNCC 4 cut(s) 258, 902, 1112, 1250
ScaI AGTACT 4 cut(s) 218, 296, 380, 476
SchI GAGTC 2 cut(s) 320, 517
ScrFI CCNGG 5 cut(s) 45, 46, 278, 528, 625
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 1 cut(s) 898
SfcI CTRYAG 3 cut(s) 826, 1171, 1359
Sfr274I CTCGAG 1 cut(s) 1331
SinI GGWCC 2 cut(s) 902, 1250
SlaI CTCGAG 1 cut(s) 1331
SmaI CCCGGG 1 cut(s) 46
SmiMI CAYNNNNRTG 4 cut(s) 637, 1144, 1187, 1393
SmlI CTYRAG 3 cut(s) 1223, 1331, 1378
SmoI CTYRAG 3 cut(s) 1223, 1331, 1378
SphI GCATGC 2 cut(s) 146, 440
Sse9I AATT 3 cut(s) 566, 789, 1116
SspMI CTAG 3 cut(s) 729, 864, 1004
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 43, 44, 276, 526, 623
StyI CCWWGG 3 cut(s) 78, 330, 1155
TaaI ACNGT 5 cut(s) 205, 598, 1189, 1245, 1250
TaqI TCGA 1 cut(s) 1332
TaqII GACCGA 2 cut(s) 371, 968
TasI AATT 3 cut(s) 566, 789, 1116
TatI WGTACW 6 cut(s) 174, 216, 294, 378, 456, 474
TauI GCSGC 7 cut(s) 74, 77, 86, 89, 263, 510, 1023
TfiI GAWTC 2 cut(s) 809, 1280
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 3 cut(s) 238, 639, 1282
TseFI GTSAC 5 cut(s) 323, 571, 866, 1135, 1189
TseI GCWGC 7 cut(s) 195, 263, 440, 504, 825, 1091, 1361
Tsp45I GTSAC 5 cut(s) 323, 571, 866, 1135, 1189
TspDTI ATGAA 4 cut(s) 477, 804, 1092, 1134
TspGWI ACGGA 1 cut(s) 533
TspMI CCCGGG 1 cut(s) 44
TspRI CASTG 3 cut(s) 238, 639, 1282
VpaK11BI GGWCC 2 cut(s) 902, 1250
XagI CCTNNNNNAGG 1 cut(s) 497
XceI RCATGY 3 cut(s) 146, 440, 565
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XhoI CTCGAG 1 cut(s) 1331
XmaI CCCGGG 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 600, 1341
XspI CTAG 3 cut(s) 729, 864, 1004
ZrmI AGTACT 4 cut(s) 218, 296, 380, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.