Rh7BG148000

Endoglucanase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0008244
Physical Location & Seq
Reverse (-)
10999606 .. 11003766
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG148000.1

Sequence Viewer

Length: 1518 bp
ATGGAGAAATTTGTGAGACTCATTTCCATGGCTCCTCTGTTTCTGCTTCTCTGCTTTCCTCTGGCTCTGGCTGGTCATGACTATGGCGCAGCTCTCAGCAAGAGCATTCTCTTCTTTGAAGCTCAGAGATCTGGTGTCCTTCCCCGTAACCAAAGGGTCACTTGGAGATCCAACTCCGGCTTGTATGATGGCAAGACCAGCGGGGTGGATCTTGTTGGGGGTTACTATGACGCAGGGGACAATGTGAAATTTGGGCTTCCCATGGCGTTCACTGTTACTATGATGTCCTGGAGTATAATAGAGTACGGAAAGCAAATGGCTTCAAGCGGTGAACTTGGGCACGCCATGGACGCTGTCAAGTGGGGCACTGACTACTTCATTAAAGCTCACCCAGAACCCAACGTTCTCTATGGAGAGGTCGGAGACGGGAACAGTGACCACTACTGCTGGCAGAGGCCGGAGGACATGACCACCGACCGCCACGCTTACAAGATCAGCCCGAGTAATCCCGGGTCGGATCTCGCCGGAGAAACCGCCGCCGCCATGGCCGCCGCCTCCATTGTGTTCCACCGCACCAACCCCGCCTACTCCCGCGAGCTCTTACAGCATGCGTACCAGCTGTTTGATTTTGCAGACAAGTACAGGGGCAAATATGACAGCAGCATTACAGTTGCTCAAAAGTACTACCGATCCATCAGTGGCTACAATGATGAGTTGCTTTGGGCCGCTGCTTGGTTGTACCAGGCTTCCAACAATGAGTACTACTTGAACTACCTTGCTAACAATGGTGACTCCATGGGTGGAACTGGTTGGGGCATGACCGAGTTTGGTTGGGATGTCAAGTACTCTGGTGTTCAGACCCTTGTTGCTAAGTTCTTAATGCAAGGCAAAGCTGGTAAGCATGCTGCTGTCTTTGAGAAGTACACGGCGAAAGCTGAGTACTTTATGTGTTCATGCCTCGGAAAGGGCAGCCGCAATGTGCAGAAGACTCCTGGTGGCCTCATTTTCCGTCAAAGGTGGAACAACATGCAATTTGTGACAAGTGCCTCCTTCTTGGCCACAGTCTACTCCGACTATCTTACATCTTCCAGGAGAACACTGAAGTGTGCTTCTGGCAATGTGGCACCTACTGAGCTTCTATCCTTTGCAAAATCTCAGGTGGACTACATTCTCGGAGACAACCCAAGAGCCACTAGCTACATGGTTGGATATGGAAACAACTATCCTCAACAAGTTCACCACAGGGCTTCCTCAATTGTTTCCATCAAGAAGGATTCTTCATTTGTGAGCTGCAGGGGAGGTTATGCTACTTGGTTTAGCAGGAAGGCTAGTGACCCCAATCTCCTTACGGGTGCTATTGTTGGTGGACCTGATGCCTATGACAACTTTGCTGATCAGAGGGACAACTATGAGCAGACCGAACCTGCTACCTACAACAATGCTCCTCTTATCGGTTTATTGGCTCGTCTAGGAGGTGGTCAGAGCGGCTATAACCAGCTTCTTCCAGGTATATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 map00500 map01100
Pfam Domains
Protein Families
CBM49 Glyco_hydro_9

Protein Analysis

505

Amino Acids

55.83

Weight (kDa)

8.02

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_9 PF00759 28 - 487 1.8e-168 Glycosyl hydrolase family 9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1432
AccB1I GGYRCC 1 cut(s) 1123
AccBSI CCGCTC 1 cut(s) 1485
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 1 cut(s) 594
AclI AACGTT 1 cut(s) 402
AclWI GGATC 4 cut(s) 162, 216, 525, 684
AcoI YGGCCR 2 cut(s) 546, 1056
AcsI RAATTY 2 cut(s) 8, 248
AcuI CTGAAG 1 cut(s) 1121
AfaI GTAC 9 cut(s) 305, 614, 641, 683, 740, 761, 845, 923, 941
AfiI CCNNNNNNNGG 3 cut(s) 732, 964, 1451
AgsI TTSAA 3 cut(s) 119, 324, 769
AjnI CCWGG 5 cut(s) 287, 741, 991, 1088, 1504
AleI CACNNNNGTG 1 cut(s) 1102
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 3 cut(s) 10, 417, 1170
AlwI GGATC 4 cut(s) 162, 216, 525, 684
Ama87I CYCGRG 2 cut(s) 499, 509
AoxI GGCC 5 cut(s) 455, 546, 723, 997, 1056
ApeKI GCWGC 6 cut(s) 89, 660, 728, 905, 969, 1290
ApoI RAATTY 2 cut(s) 8, 248
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 723, 1367
AsuC2I CCSGG 2 cut(s) 510, 511
AsuHPI GGTGA 4 cut(s) 341, 380, 800, 1229
AvaI CYCGRG 2 cut(s) 499, 509
AvaII GGWCC 1 cut(s) 1367
BaeGI GKGCMC 2 cut(s) 342, 368
BalI TGGCCA 1 cut(s) 1058
BanI GGYRCC 1 cut(s) 1123
BanII GRGCYC 1 cut(s) 600
BbsI GAAGAC 1 cut(s) 992
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 6 cut(s) 101, 672, 715, 892, 981, 1277
BccI CCATC 3 cut(s) 182, 701, 1271
BceAI ACGGC 1 cut(s) 942
BciT130I CCWGG 5 cut(s) 289, 743, 993, 1090, 1506
BclI TGATCA 1 cut(s) 1393
BcnI CCSGG 2 cut(s) 510, 511
BcoDI GTCTC 3 cut(s) 10, 417, 1170
BfaI CTAG 3 cut(s) 1194, 1329, 1469
BfmI CTRYAG 1 cut(s) 1291
BfuAI ACCTGC 1 cut(s) 1432
BglI GCCNNNNNGGC 1 cut(s) 545
BglII AGATCT 1 cut(s) 128
BmcAI AGTACT 4 cut(s) 683, 761, 845, 941
Bme1390I CCNGG 7 cut(s) 289, 510, 511, 743, 993, 1090, 1506
Bme18I GGWCC 1 cut(s) 1367
BmeT110I CYCGRG 2 cut(s) 499, 509
BmgT120I GGNCC 2 cut(s) 723, 1367
BmiI GGNNCC 2 cut(s) 33, 1125
BmrFI CCNGG 7 cut(s) 289, 510, 511, 743, 993, 1090, 1506
BmsI GCATC 1 cut(s) 1363
BpiI GAAGAC 1 cut(s) 992
BpmI CTGGAG 1 cut(s) 310
BpuMI CCSGG 2 cut(s) 510, 511
BsaJI CCNNGG 7 cut(s) 27, 261, 345, 509, 543, 795, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 732, 964, 1451
Bse1I ACTGG 1 cut(s) 811
Bse3DI GCAATG 2 cut(s) 982, 1123
BseBI CCWGG 5 cut(s) 289, 743, 993, 1090, 1506
BseDI CCNNGG 7 cut(s) 27, 261, 345, 509, 543, 795, 958
BseGI GGATG 1 cut(s) 841
BseLI CCNNNNNNNGG 3 cut(s) 732, 964, 1451
BseMI GCAATG 2 cut(s) 982, 1123
BseMII CTCAG 5 cut(s) 109, 137, 927, 1122, 1169
BseNI ACTGG 1 cut(s) 811
BseRI GAGGAG 2 cut(s) 24, 1434
BseSI GKGCMC 2 cut(s) 342, 368
BseXI GCAGC 6 cut(s) 101, 672, 715, 892, 981, 1277
BsgI GTGCAG 1 cut(s) 1001
Bsh1236I CGCG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 5 cut(s) 457, 548, 725, 999, 1058
BshNI GGYRCC 1 cut(s) 1123
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 2 cut(s) 499, 509
BsiSI CCGG 4 cut(s) 177, 458, 510, 525
BslFI GGGAC 2 cut(s) 251, 1415
BslI CCNNNNNNNGG 3 cut(s) 732, 964, 1451
BsmAI GTCTC 3 cut(s) 10, 417, 1170
BsmBI CGTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 251, 1415
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 5 cut(s) 457, 548, 725, 999, 1058
BsoBI CYCGRG 2 cut(s) 499, 509
Bsp1286I GDGCHC 3 cut(s) 342, 368, 600
Bsp143I GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
Bsp19I CCATGG 5 cut(s) 27, 261, 345, 543, 795
BspANI GGCC 5 cut(s) 457, 548, 725, 999, 1058
BspCNI CTCAG 5 cut(s) 108, 136, 928, 1123, 1168
BspFNI CGCG 1 cut(s) 594
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 33, 1125
BspMAI CTGCAG 1 cut(s) 1295
BspMI ACCTGC 1 cut(s) 1432
BspPI GGATC 4 cut(s) 162, 216, 525, 684
BspT107I GGYRCC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 1485
BsrDI GCAATG 2 cut(s) 982, 1123
BsrI ACTGG 1 cut(s) 811
BssECI CCNNGG 7 cut(s) 27, 261, 345, 509, 543, 795, 958
BssMI GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
BssT1I CCWWGG 5 cut(s) 27, 261, 345, 543, 795
Bst2UI CCWGG 5 cut(s) 289, 743, 993, 1090, 1506
Bst4CI ACNGT 4 cut(s) 274, 434, 670, 1063
Bst6I CTCTTC 1 cut(s) 116
BstC8I GCNNGC 5 cut(s) 342, 449, 596, 609, 903
BstDEI CTNAG 6 cut(s) 95, 123, 870, 936, 1131, 1155
BstDSI CCRYGG 5 cut(s) 27, 261, 345, 543, 795
BstENI CCTNNNNNAGG 1 cut(s) 962
BstF5I GGATG 1 cut(s) 841
BstFNI CGCG 1 cut(s) 594
BstHHI GCGC 1 cut(s) 89
BstKTI GATC 7 cut(s) 131, 170, 211, 495, 520, 692, 1396
BstMAI GTCTC 3 cut(s) 10, 417, 1170
BstMBI GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 5 cut(s) 198, 350, 545, 548, 604
BstNI CCWGG 5 cut(s) 289, 743, 993, 1090, 1506
BstNSI RCATGY 3 cut(s) 611, 905, 1030
BstSCI CCNGG 7 cut(s) 287, 508, 509, 741, 991, 1088, 1504
BstSFI CTRYAG 1 cut(s) 1291
BstSLI GKGCMC 2 cut(s) 342, 368
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 6 cut(s) 101, 672, 715, 892, 981, 1277
BstV2I GAAGAC 1 cut(s) 992
BstX2I RGATCY 4 cut(s) 128, 167, 208, 517
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 4 cut(s) 128, 167, 208, 517
BsuRI GGCC 5 cut(s) 457, 548, 725, 999, 1058
BtgI CCRYGG 5 cut(s) 27, 261, 345, 543, 795
BtsCI GGATG 1 cut(s) 841
BtsIMutI CAGTG 5 cut(s) 270, 366, 439, 703, 1097
BveI ACCTGC 1 cut(s) 1432
Cac8I GCNNGC 5 cut(s) 342, 449, 596, 609, 903
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 723, 1367
Cfr9I CCCGGG 1 cut(s) 509
CseI GACGC 2 cut(s) 239, 359
Csp6I GTAC 9 cut(s) 304, 613, 640, 682, 739, 760, 844, 922, 940
CviQI GTAC 9 cut(s) 304, 613, 640, 682, 739, 760, 844, 922, 940
DdeI CTNAG 6 cut(s) 95, 123, 870, 936, 1131, 1155
DpnI GATC 7 cut(s) 130, 169, 210, 494, 519, 691, 1395
DpnII GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
EaeI YGGCCR 2 cut(s) 546, 1056
Eam1104I CTCTTC 1 cut(s) 116
EarI CTCTTC 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 598
Eco130I CCWWGG 5 cut(s) 27, 261, 345, 543, 795
Eco24I GRGCYC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 1367
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 1121
Eco88I CYCGRG 2 cut(s) 499, 509
EcoICRI GAGCTC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 962
EcoRII CCWGG 5 cut(s) 287, 741, 991, 1088, 1504
EcoT14I CCWWGG 5 cut(s) 27, 261, 345, 543, 795
EcoT38I GRGCYC 1 cut(s) 600
ErhI CCWWGG 5 cut(s) 27, 261, 345, 543, 795
Esp3I CGTCTC 1 cut(s) 417
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 2 cut(s) 251, 1415
FauI CCCGC 3 cut(s) 194, 589, 599
FbaI TGATCA 1 cut(s) 1393
FblI GTMKAC 1 cut(s) 1065
FokI GGATG 1 cut(s) 848
FriOI GRGCYC 1 cut(s) 600
FspBI CTAG 3 cut(s) 1194, 1329, 1469
GlaI GCGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 310
HaeIII GGCC 5 cut(s) 457, 548, 725, 999, 1058
HapII CCGG 4 cut(s) 177, 458, 510, 525
HgaI GACGC 2 cut(s) 239, 359
HhaI GCGC 1 cut(s) 89
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HinfI GANTC 4 cut(s) 18, 791, 988, 1274
HpaII CCGG 4 cut(s) 177, 458, 510, 525
HphI GGTGA 4 cut(s) 341, 380, 800, 1229
Hpy166II GTNNAC 7 cut(s) 270, 332, 924, 1066, 1162, 1237, 1367
Hpy188I TCNGA 9 cut(s) 126, 422, 517, 858, 962, 1072, 1175, 1398, 1482
Hpy188III TCNNGA 2 cut(s) 77, 1267
Hpy8I GTNNAC 7 cut(s) 270, 332, 924, 1066, 1162, 1237, 1367
HpyAV CCTTC 4 cut(s) 149, 1060, 1264, 1318
HpyCH4III ACNGT 4 cut(s) 274, 434, 670, 1063
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 6 cut(s) 632, 883, 982, 1030, 1148, 1293
HpyF10VI GCNNNNNNNGC 5 cut(s) 198, 350, 545, 548, 604
HpyF3I CTNAG 6 cut(s) 95, 123, 870, 936, 1131, 1155
HpySE526I ACGT 1 cut(s) 402
HspAI GCGC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 1393
Kzo9I GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
LmnI GCTCC 2 cut(s) 37, 1447
Lsp1109I GCAGC 6 cut(s) 101, 672, 715, 892, 981, 1277
LweI GCATC 1 cut(s) 1363
MaeI CTAG 3 cut(s) 1194, 1329, 1469
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 8 cut(s) 146, 157, 221, 274, 434, 788, 1036, 1331
MalI GATC 7 cut(s) 130, 169, 210, 494, 519, 691, 1395
MbiI CCGCTC 1 cut(s) 1485
MboI GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
MboII GAAGA 5 cut(s) 103, 997, 1077, 1269, 1493
MfeI CAATTG 1 cut(s) 1254
MflI RGATCY 4 cut(s) 128, 167, 208, 517
MhlI GDGCHC 3 cut(s) 342, 368, 600
MlsI TGGCCA 1 cut(s) 1058
MluCI AATT 4 cut(s) 8, 248, 1031, 1254
MluNI TGGCCA 1 cut(s) 1058
MlyI GAGTC 3 cut(s) 12, 785, 982
MmeI TCCRAC 6 cut(s) 195, 400, 495, 774, 1095, 1186
Mox20I TGGCCA 1 cut(s) 1058
MscI TGGCCA 1 cut(s) 1058
MseI TTAA 2 cut(s) 381, 878
MslI CAYNNNNRTG 3 cut(s) 26, 81, 1102
Msp20I TGGCCA 1 cut(s) 1058
MspA1I CMGCKG 3 cut(s) 201, 619, 728
MspI CCGG 4 cut(s) 177, 458, 510, 525
MspR9I CCNGG 7 cut(s) 289, 510, 511, 743, 993, 1090, 1506
MunI CAATTG 1 cut(s) 1254
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 5 cut(s) 289, 743, 993, 1090, 1506
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 5 cut(s) 198, 350, 545, 548, 604
NciI CCSGG 2 cut(s) 510, 511
NcoI CCATGG 5 cut(s) 27, 261, 345, 543, 795
NdeII GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
NlaIV GGNNCC 2 cut(s) 33, 1125
NmuCI GTSAC 5 cut(s) 157, 434, 788, 1036, 1331
NspI RCATGY 3 cut(s) 611, 905, 1030
OliI CACNNNNGTG 1 cut(s) 1102
PaeI GCATGC 2 cut(s) 611, 905
PagI TCATGA 1 cut(s) 76
PctI GAATGC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 1274
PflFI GACNNNGTC 1 cut(s) 353
PfoI TCCNGGA 2 cut(s) 287, 1088
PleI GAGTC 3 cut(s) 12, 785, 982
PpsI GAGTC 3 cut(s) 12, 785, 982
Psp124BI GAGCTC 1 cut(s) 600
Psp1406I AACGTT 1 cut(s) 402
Psp6I CCWGG 5 cut(s) 287, 741, 991, 1088, 1504
PspGI CCWGG 5 cut(s) 287, 741, 991, 1088, 1504
PspN4I GGNNCC 2 cut(s) 33, 1125
PspPI GGNCC 2 cut(s) 723, 1367
PstI CTGCAG 1 cut(s) 1295
PsuI RGATCY 4 cut(s) 128, 167, 208, 517
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 619
RsaI GTAC 9 cut(s) 305, 614, 641, 683, 740, 761, 845, 923, 941
RsaNI GTAC 9 cut(s) 304, 613, 640, 682, 739, 760, 844, 922, 940
RseI CAYNNNNRTG 3 cut(s) 26, 81, 1102
SacI GAGCTC 1 cut(s) 600
SaqAI TTAA 2 cut(s) 381, 878
Sau3AI GATC 7 cut(s) 128, 167, 208, 492, 517, 689, 1393
Sau96I GGNCC 2 cut(s) 723, 1367
ScaI AGTACT 4 cut(s) 683, 761, 845, 941
SchI GAGTC 3 cut(s) 12, 785, 982
ScrFI CCNGG 7 cut(s) 289, 510, 511, 743, 993, 1090, 1506
SduI GDGCHC 3 cut(s) 342, 368, 600
SfaNI GCATC 1 cut(s) 1363
SfcI CTRYAG 1 cut(s) 1291
SinI GGWCC 1 cut(s) 1367
SmaI CCCGGG 1 cut(s) 511
SmiMI CAYNNNNRTG 3 cut(s) 26, 81, 1102
SphI GCATGC 2 cut(s) 611, 905
Sse9I AATT 4 cut(s) 8, 248, 1031, 1254
SspMI CTAG 3 cut(s) 1194, 1329, 1469
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 7 cut(s) 287, 508, 509, 741, 991, 1088, 1504
StyI CCWWGG 5 cut(s) 27, 261, 345, 543, 795
TaaI ACNGT 4 cut(s) 274, 434, 670, 1063
TaiI ACGT 1 cut(s) 405
TaqII GACCGA 2 cut(s) 836, 1433
TasI AATT 4 cut(s) 8, 248, 1031, 1254
TatI WGTACW 6 cut(s) 639, 681, 759, 843, 921, 939
TauI GCSGC 7 cut(s) 539, 542, 551, 554, 728, 975, 1488
TfiI GAWTC 1 cut(s) 1274
Tru1I TTAA 2 cut(s) 381, 878
Tru9I TTAA 2 cut(s) 381, 878
TscAI CASTG 5 cut(s) 277, 373, 439, 703, 1104
TseFI GTSAC 5 cut(s) 157, 434, 788, 1036, 1331
TseI GCWGC 6 cut(s) 89, 660, 728, 905, 969, 1290
Tsp45I GTSAC 5 cut(s) 157, 434, 788, 1036, 1331
TspDTI ATGAA 3 cut(s) 367, 942, 1269
TspGWI ACGGA 2 cut(s) 321, 998
TspMI CCCGGG 1 cut(s) 509
TspRI CASTG 5 cut(s) 277, 373, 439, 703, 1104
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 1367
XagI CCTNNNNNAGG 1 cut(s) 962
XapI RAATTY 2 cut(s) 8, 248
XceI RCATGY 3 cut(s) 611, 905, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 1198
XmaI CCCGGG 1 cut(s) 509
XmiI GTMKAC 1 cut(s) 1065
XspI CTAG 3 cut(s) 1194, 1329, 1469
ZrmI AGTACT 4 cut(s) 683, 761, 845, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.