pycom06g10660

Dof zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012750
Physical Location & Seq
Forward (+)
15950972 .. 15952371
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g10660.1

Sequence Viewer

Length: 945 bp
ATGGATACTGCTCAGTGGCCACAGGGAATTGTGGTGAAACCAATAGAGGAGATAGTGACAAACTCATGCCCTAAGCCTGCAGCAGCTAATAATTTGGAGAGGAAAGCAAGGCCTCAGAAGGAACAAGCCCTAAACTGTCCAAGGTGCAACTCCACCAACACGAAATTCTGCTACTACAACAACTACAGCCTCACACAACCAAGGTACTTCTGCAAGACTTGCAGAAGGTACTGGACTGAAGGTGGGTCCCTCAGAAGCATCCCAGTTGGAGGTGGTTCAAGGAAGAACAAGAGATCTTCATCCTCAAACAATTCTCCATCAACAACTTCTAACAATTCTTCTTCTTCCTCAGCATCCAAAAGGCTTCCTGATCTGATTCACCAAAACCCTAAAATCAACCAAGCCCAAGATCTGAACCTCACTTTCCCATCCACTAATCAAGATTTCAGGAATATTATCTCTGGTCACTTGCTTAATCACCATCATCAACAACTTCCAAACAACAACATCAATGATCAAAGCACCAACAAGCAGCAAAACCACATCTCTACTTCTTCCCCAACTTCTACTACTACAACTACAACAACATCCCACCTTTCTGCCTTAGAGCTTCTCACTGGATTGACTTCTAGGGGTTTGAACTCCTTCATGCCTATGCCAGTCCTATCAGACCCGAACAACAACAACTCTGTATATAATAGTCCTTCTGGACTAATTAATCCGATGCAGGATTTCAAGCCAACTCTTAATTTTTCACTGGATGGGCTTGGAAGTGGATATGGGAGTCTTCAAGGTCATCATGTTCAACAGAGCAGTGGGAGGCTTTTGTTTCCCTTTGAAGATTTGAAACAAGCTCCTTCAGGAGGAGGGATTAATGAGCAGAACAAGCAGCATGGAGATTCAACTGGGTATTGGACAGGAATGCTAGGAGGAGGATCATGGTAA

Protein Analysis

315

Amino Acids

34.4

Weight (kDa)

9.33

Isoelectric Point (pI)

56.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 42 - 98 3.5e-33 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24060
fragaria_vesca FvH4_5g11410
malus_domestica MD06G1114000.v1.1 MD14G1135300.v1.1
prunus_persica Prupe.5G126800_v2.0.a1
pyrus_communis pycom06g10660 pycom14g10850
rosa_chinensis RchiOBHm_Chr7g0186471
rosa_laevigata RLG00000004843
rosa_multiflora Rmu_sc0000645.1_g000033
rosa_roxburghii Rroxscaffold_3G00268120
rosa_rugosa Rorug06G0474500
rosa_samantha Rh7AG066800 Rh7BG082600 Rh7CG081100 Rh7DG081800
rosa_wichuraiana Rw7G006800 Rw7G007040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 943
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 1 cut(s) 164
AcuI CTGAAG 2 cut(s) 258, 843
AfaI GTAC 2 cut(s) 206, 230
AfiI CCNNNNNNNGG 2 cut(s) 269, 863
AgsI TTSAA 8 cut(s) 279, 640, 736, 791, 806, 839, 847, 903
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 3 cut(s) 86, 610, 854
AluI AGCT 3 cut(s) 86, 610, 854
AlwI GGATC 1 cut(s) 943
AoxI GGCC 2 cut(s) 17, 110
ApeKI GCWGC 4 cut(s) 80, 83, 532, 889
ApoI RAATTY 1 cut(s) 164
AseI ATTAAT 2 cut(s) 717, 873
Asp700I GAANNNNTTC 1 cut(s) 644
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 3 cut(s) 46, 371, 470
AvaII GGWCC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 779
BbvCI CCTCAGC 1 cut(s) 349
BbvI GCAGC 4 cut(s) 92, 95, 544, 901
BccI CCATC 4 cut(s) 325, 436, 489, 755
BclI TGATCA 1 cut(s) 514
BfaI CTAG 2 cut(s) 630, 926
BfmI CTRYAG 2 cut(s) 78, 184
BglII AGATCT 2 cut(s) 293, 409
BisI GCNGC 4 cut(s) 81, 84, 533, 890
BlsI GCNGC 4 cut(s) 82, 85, 534, 891
Bme18I GGWCC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 247, 248
BmrI ACTGGG 2 cut(s) 257, 915
BmsI GCATC 3 cut(s) 267, 362, 714
BmuI ACTGGG 2 cut(s) 257, 915
BpiI GAAGAC 1 cut(s) 779
Bpu10I CCTNAGC 2 cut(s) 72, 349
BsaBI GATNNNNATC 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 140, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 863
Bse1I ACTGG 6 cut(s) 236, 263, 622, 659, 762, 910
Bse8I GATNNNNATC 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 140, 200
BseGI GGATG 6 cut(s) 258, 299, 353, 428, 587, 766
BseJI GATNNNNATC 1 cut(s) 298
BseLI CCNNNNNNNGG 2 cut(s) 269, 863
BseMII CTCAG 4 cut(s) 26, 128, 265, 363
BseNI ACTGG 6 cut(s) 236, 263, 622, 659, 762, 910
BseRI GAGGAG 3 cut(s) 62, 879, 945
BseXI GCAGC 4 cut(s) 92, 95, 544, 901
BshFI GGCC 2 cut(s) 19, 112
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 269, 863
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 927
BsnI GGCC 2 cut(s) 19, 112
Bsp143I GATC 5 cut(s) 293, 370, 409, 514, 935
BspANI GGCC 2 cut(s) 19, 112
BspCNI CTCAG 4 cut(s) 25, 127, 264, 362
BspLI GGNNCC 2 cut(s) 247, 248
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 943
BsrI ACTGG 6 cut(s) 236, 263, 622, 659, 762, 910
BssECI CCNNGG 2 cut(s) 140, 200
BssMI GATC 5 cut(s) 293, 370, 409, 514, 935
BssT1I CCWWGG 2 cut(s) 140, 200
Bst4CI ACNGT 1 cut(s) 137
BstAPI GCANNNNNTGC 1 cut(s) 219
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 6 cut(s) 12, 72, 114, 251, 349, 604
BstENI CCTNNNNNAGG 1 cut(s) 861
BstF5I GGATG 6 cut(s) 258, 299, 353, 428, 587, 766
BstKTI GATC 5 cut(s) 296, 373, 412, 517, 938
BstMBI GATC 5 cut(s) 293, 370, 409, 514, 935
BstMWI GCNNNNNNNGC 2 cut(s) 219, 886
BstSFI CTRYAG 2 cut(s) 78, 184
BstV1I GCAGC 4 cut(s) 92, 95, 544, 901
BstV2I GAAGAC 1 cut(s) 779
BstX2I RGATCY 2 cut(s) 293, 409
BstYI RGATCY 2 cut(s) 293, 409
BsuRI GGCC 2 cut(s) 19, 112
BtsCI GGATG 6 cut(s) 258, 299, 353, 428, 587, 766
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 4 cut(s) 20, 615, 755, 820
Cac8I GCNNGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 205, 229
CviAII CATG 5 cut(s) 66, 649, 800, 893, 939
CviQI GTAC 2 cut(s) 205, 229
DdeI CTNAG 6 cut(s) 12, 72, 114, 251, 349, 604
DpnI GATC 5 cut(s) 295, 372, 411, 516, 937
DpnII GATC 5 cut(s) 293, 370, 409, 514, 935
EaeI YGGCCR 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 140, 200
Eco147I AGGCCT 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 246
Eco57I CTGAAG 2 cut(s) 258, 843
EcoNI CCTNNNNNAGG 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 246
EcoT14I CCWWGG 2 cut(s) 140, 200
ErhI CCWWGG 2 cut(s) 140, 200
FaeI CATG 5 cut(s) 69, 652, 803, 896, 942
FaiI YATR 9 cut(s) 67, 650, 656, 694, 696, 780, 801, 894, 940
FaqI GGGAC 1 cut(s) 232
FatI CATG 5 cut(s) 65, 648, 799, 892, 938
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 4 cut(s) 81, 84, 533, 890
FokI GGATG 6 cut(s) 245, 286, 340, 415, 574, 773
Fsp4HI GCNGC 4 cut(s) 81, 84, 533, 890
FspBI CTAG 2 cut(s) 630, 926
GluI GCNGC 4 cut(s) 81, 84, 533, 890
HaeIII GGCC 2 cut(s) 19, 112
Hin1II CATG 5 cut(s) 69, 652, 803, 896, 942
HinfI GANTC 3 cut(s) 376, 784, 899
HphI GGTGA 3 cut(s) 46, 371, 470
Hpy188I TCNGA 6 cut(s) 117, 254, 375, 414, 670, 723
Hpy188III TCNNGA 5 cut(s) 368, 440, 448, 708, 861
HpyAV CCTTC 6 cut(s) 112, 219, 233, 655, 714, 867
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4V TGCA 5 cut(s) 80, 147, 213, 222, 727
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 886
HpyF3I CTNAG 6 cut(s) 12, 72, 114, 251, 349, 604
Hsp92II CATG 5 cut(s) 69, 652, 803, 896, 942
KflI GGGWCCC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 5 cut(s) 293, 370, 409, 514, 935
LmnI GCTCC 1 cut(s) 859
Lsp1109I GCAGC 4 cut(s) 92, 95, 544, 901
LweI GCATC 3 cut(s) 267, 362, 714
MaeI CTAG 2 cut(s) 630, 926
MaeIII GTNAC 2 cut(s) 55, 464
MalI GATC 5 cut(s) 295, 372, 411, 516, 937
MboI GATC 5 cut(s) 293, 370, 409, 514, 935
MboII GAAGA 8 cut(s) 288, 295, 330, 333, 336, 546, 779, 851
MflI RGATCY 2 cut(s) 293, 409
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 7 cut(s) 27, 91, 164, 310, 334, 714, 748
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 1 cut(s) 793
MmeI TCCRAC 1 cut(s) 247
Mox20I TGGCCA 1 cut(s) 19
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 19
MseI TTAA 4 cut(s) 474, 717, 747, 873
MslI CAYNNNNRTG 1 cut(s) 653
Msp20I TGGCCA 1 cut(s) 19
Mva1269I GAATGC 1 cut(s) 927
MwoI GCNNNNNNNGC 2 cut(s) 219, 886
NdeII GATC 5 cut(s) 293, 370, 409, 514, 935
NlaIII CATG 5 cut(s) 69, 652, 803, 896, 942
NlaIV GGNNCC 2 cut(s) 247, 248
NmuCI GTSAC 2 cut(s) 55, 464
PceI AGGCCT 1 cut(s) 112
PctI GAATGC 1 cut(s) 927
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 376, 899
PkrI GCNGC 4 cut(s) 82, 85, 534, 891
PleI GAGTC 1 cut(s) 792
PpsI GAGTC 1 cut(s) 792
PpuMI RGGWCCY 1 cut(s) 246
PshBI ATTAAT 2 cut(s) 717, 873
Psp5II RGGWCCY 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 247, 248
PspPI GGNCC 1 cut(s) 246
PspPPI RGGWCCY 1 cut(s) 246
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 293, 409
RsaI GTAC 2 cut(s) 206, 230
RsaNI GTAC 2 cut(s) 205, 229
RseI CAYNNNNRTG 1 cut(s) 653
SaqAI TTAA 4 cut(s) 474, 717, 747, 873
SatI GCNGC 4 cut(s) 81, 84, 533, 890
Sau3AI GATC 5 cut(s) 293, 370, 409, 514, 935
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 1 cut(s) 793
SfaNI GCATC 3 cut(s) 267, 362, 714
SfcI CTRYAG 2 cut(s) 78, 184
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 653
Sse9I AATT 7 cut(s) 27, 91, 164, 310, 334, 714, 748
SseBI AGGCCT 1 cut(s) 112
SspI AATATT 1 cut(s) 454
SspMI CTAG 2 cut(s) 630, 926
StuI AGGCCT 1 cut(s) 112
StyI CCWWGG 2 cut(s) 140, 200
TaaI ACNGT 1 cut(s) 137
TasI AATT 7 cut(s) 27, 91, 164, 310, 334, 714, 748
TfiI GAWTC 2 cut(s) 376, 899
Tru1I TTAA 4 cut(s) 474, 717, 747, 873
Tru9I TTAA 4 cut(s) 474, 717, 747, 873
TscAI CASTG 4 cut(s) 20, 622, 762, 820
TseFI GTSAC 2 cut(s) 55, 464
TseI GCWGC 4 cut(s) 80, 83, 532, 889
Tsp45I GTSAC 2 cut(s) 55, 464
TspDTI ATGAA 2 cut(s) 288, 637
TspRI CASTG 4 cut(s) 20, 622, 762, 820
VpaK11BI GGWCC 1 cut(s) 246
VspI ATTAAT 2 cut(s) 717, 873
XagI CCTNNNNNAGG 1 cut(s) 861
XapI RAATTY 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 2 cut(s) 630, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.