pycom06g13060

Non-specific phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011304
Physical Location & Seq
Forward (+)
18141768 .. 18144124
2357 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13060.2

Sequence Viewer

Length: 1590 bp
ATGGGAGCTTCCAAGACCAAAACTTTCCCACATTTTCCATCGTTTTCCTTCGTTTTCTCACTCTTTCTCACTCTCTCATCGACTTTCCGGCCAACCCATTTCTGCTCTGCCCAGCAGCAGCAACCCATCAAAACCATCGTCGTTTTGGTCATGGAAAACCGCTCCTTCGACCACATACTGGGTTGGATGAAAAAATCCGTTAACCCAGCAATCAACGGCGTCACTGGCAAAGAATGCAACCCGGTTTCAACCAAACCCGCCGACCCGGAAAAATCCATCTGTTTCTCCGACGACGCCGAGTTCGTGGACCCGGATCCGGGTCACTCATTCGAAGCGGTCGAGCAGCAGGTATTCGGGTCGGGTTCCCACCCTTCGATGACCGGGTTCGTCGAACAAGCATTGTCAATGTCCCCAAACCTCTCCGAAACCGTCATGAAAGGCTTCAGACCGGAATCCGTTCCGGTCTACGCGGCCCTGGTCAGCGAATTCGCGGTGTTCGACCGGTGGTTCTCTTCAATTCCCGGTCCGACTCAACCCAACCGGCTGTTCGTCTACTCCGCAACTTCCCACGGCTCGACCAGCCACGTCAAGAAACAATTAGCTTCCGGGTACCCACAAAAAACAATCTTCGATTCGGTCCATGAAAACGGGTTGGATTTCGGTGTCTACTTCCAGAACATCCCGACAACAATGTTCTACCGAAACATGCGGAAACTGAAGTACATTTTCAAGTTCCACCAGTACGATTTGAAGTTCAAGAAAGATGCTCGGAAGGGGAAGCTCCCGAGCTTGACGGTGATCGAACCGCGTTACTTCGACCTCAAGGGCATGCCGGCGAACGACGACCACCCGTCCCATGATGTCGCCAACGGACAGAGGCTGGTTAAGGATGTTTACGAGACGTTGAGGGCGAGTCCTCAGTGGAACGACACGCTTTTGGTGATCACGTACGACGAGCATGGCGGCTTTTTCGATCATGTCAAGACTCCGTATGTTAATGTTCCGAACCCGGACGGGAATAGCGGTCCGGCTCCTTCTTTTTTTAAGTTCGATCGGCTCGGAGTTCGCGTGCCGACGATTATGGTGTCGCCGTGGATTAAGAAGGGGACTGTGATGAGTGGTCCTCAAGGACCTACACCGAACTCCGAGTTTGAGCACTCTTCAATCCCCGCCACCATAAAGAAAATGTTCAACCTCAGCTCTAACTTCTTAACTCACAGAGACGCATGGGCCGGGACATTCGAGCAGGTTGTTGGGCAATTATCTTCTCCCAGAACTGACTGCCCAGTGACCTTGCCGGACGTAGTGCCTCTGAGGAAAACGGAGGCCAAGGAAGACAGTAATTTATCCGAGTTTCAGGGGGAGGTAGTGCAGTTAGGGGCCGTGCTCAACGGCGACCACTTCTTGAGCAGCTACCCGGACGAAATGAGCAAGAAGATGAGTGTGAGGGAAGCTCATGCGTACGTGAAAGGTGCAGTTTCAAGGTTCATTAGAGCAAGCAAGGAGGCTATAAAACTGGGAGCAGAAGAGTCAGCAATTGTAGATATGAGATCTTCCCTCACAACTAGGTCCTCAAATATTAAAAATTAA

Protein Analysis

530

Amino Acids

58.96

Weight (kDa)

8.42

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48610
fragaria_vesca FvH4_5g08480 FvH4_5g08480 FvH4_5g08480
malus_domestica MD06G1140600.v1.1 MD14G1156100.v1.1
prunus_persica Prupe.5G149800_v2.0.a1
pyrus_communis pycom06g13060 pycom14g12960
rosa_chinensis RchiOBHm_Chr7g0189931
rosa_laevigata RLG00000004576
rosa_multiflora Rmu_sc0014588.1_g000003 Rmu_sc0033399.1_g000001
rosa_roxburghii Rroxscaffold_3G00265040
rosa_rugosa Rorug06G0501200
rosa_samantha Rh7AG106300 Rh7BG109000 Rh7CG111100 Rh7DG109500
rosa_wichuraiana Rw7G009230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 337, 1237
Acc65I GGTACC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 609
AccB7I CCANNNNNTGG 1 cut(s) 178
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 3 cut(s) 465, 552, 666
AccII CGCG 4 cut(s) 470, 491, 808, 1068
AclWI GGATC 2 cut(s) 308, 321
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 2 cut(s) 427, 737
AcyI GRCGYC 2 cut(s) 219, 294
AfaI GTAC 5 cut(s) 611, 722, 743, 950, 1463
AfiI CCNNNNNNNGG 3 cut(s) 178, 316, 317
AgeI ACCGGT 1 cut(s) 501
AgsI TTSAA 8 cut(s) 249, 516, 730, 751, 757, 1164, 1192, 1482
AhdI GACNNNNNGTC 1 cut(s) 850
AjiI CACGTC 1 cut(s) 586
AjnI CCWGG 1 cut(s) 474
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 7 cut(s) 8, 602, 781, 789, 1200, 1413, 1454
AluI AGCT 7 cut(s) 8, 602, 781, 789, 1200, 1413, 1454
Alw21I GWGCWC 2 cut(s) 1158, 1389
Alw26I GTCTC 2 cut(s) 893, 1215
AlwI GGATC 2 cut(s) 308, 321
AlwNI CAGNNNCTG 1 cut(s) 880
Ama87I CYCGRG 1 cut(s) 784
AoxI GGCC 5 cut(s) 89, 471, 1230, 1326, 1380
ApeKI GCWGC 4 cut(s) 115, 118, 343, 1410
ApoI RAATTY 1 cut(s) 485
ArsI GACNNNNNNTTYG 4 cut(s) 123, 155, 284, 316
AsiGI ACCGGT 1 cut(s) 501
Asp700I GAANNNNTTC 3 cut(s) 440, 456, 1187
Asp718I GGTACC 1 cut(s) 609
AsuHPI GGTGA 2 cut(s) 808, 952
AsuII TTCGAA 1 cut(s) 330
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 7 cut(s) 307, 524, 637, 1025, 1121, 1130, 1569
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 609
BbsI GAAGAC 1 cut(s) 1341
Bbv12I GWGCWC 2 cut(s) 1158, 1389
BbvCI CCTCAGC 1 cut(s) 1196
BbvI GCAGC 4 cut(s) 127, 130, 355, 1422
BccI CCATC 4 cut(s) 46, 134, 143, 284
BceAI ACGGC 5 cut(s) 232, 586, 1075, 1367, 1408
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 942
BcoDI GTCTC 2 cut(s) 893, 1215
BfaI CTAG 1 cut(s) 1566
BfuAI ACCTGC 2 cut(s) 337, 1237
BglII AGATCT 1 cut(s) 1550
BisI GCNGC 6 cut(s) 116, 119, 344, 471, 964, 1411
BlsI GCNGC 6 cut(s) 117, 120, 345, 472, 965, 1412
Bme18I GGWCC 7 cut(s) 307, 524, 637, 1025, 1121, 1130, 1569
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 784
BmgBI CACGTC 1 cut(s) 586
BmiI GGNNCC 6 cut(s) 309, 315, 364, 611, 1032, 1381
BmrI ACTGGG 3 cut(s) 188, 1280, 1526
BmsI GCATC 1 cut(s) 754
BmuI ACTGGG 3 cut(s) 188, 1280, 1526
BpiI GAAGAC 1 cut(s) 1341
BplI GAGNNNNNCTC 4 cut(s) 1108, 1140, 1438, 1470
Bpu10I CCTNAGC 1 cut(s) 1196
Bpu14I TTCGAA 1 cut(s) 330
BpuEI CTTGAG 3 cut(s) 806, 1110, 1426
BsaAI YACGTR 2 cut(s) 948, 1465
BsaHI GRCGYC 2 cut(s) 219, 294
BsaJI CCNNGG 4 cut(s) 474, 568, 1091, 1329
BsaWI WCCGGW 3 cut(s) 448, 460, 501
BsaXI ACNNNNNCTCC 2 cut(s) 1053, 1083
Bsc4I CCNNNNNNNGG 3 cut(s) 178, 316, 317
Bse118I RCCGGY 3 cut(s) 501, 540, 832
Bse1I ACTGG 5 cut(s) 183, 229, 739, 1286, 1521
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 4 cut(s) 474, 568, 1091, 1329
BseGI GGATG 3 cut(s) 192, 678, 895
BseLI CCNNNNNNNGG 3 cut(s) 178, 316, 317
BseMII CTCAG 3 cut(s) 932, 1210, 1304
BseNI ACTGG 5 cut(s) 183, 229, 739, 1286, 1521
BseXI GCAGC 4 cut(s) 127, 130, 355, 1422
BseYI CCCAGC 2 cut(s) 111, 205
BsgI GTGCAG 2 cut(s) 1391, 1494
Bsh1236I CGCG 4 cut(s) 470, 491, 808, 1068
Bsh1285I CGRYCG 3 cut(s) 339, 502, 1054
BshFI GGCC 5 cut(s) 91, 473, 1232, 1328, 1382
BshNI GGYRCC 1 cut(s) 609
BshTI ACCGGT 1 cut(s) 501
BsiEI CGRYCG 3 cut(s) 339, 502, 1054
BsiHKAI GWGCWC 2 cut(s) 1158, 1389
BsiHKCI CYCGRG 1 cut(s) 784
BsiWI CGTACG 2 cut(s) 948, 1461
BslFI GGGAC 4 cut(s) 394, 838, 1120, 1249
BslI CCNNNNNNNGG 3 cut(s) 178, 316, 317
BsmAI GTCTC 2 cut(s) 893, 1215
BsmBI CGTCTC 2 cut(s) 893, 1215
BsmFI GGGAC 4 cut(s) 394, 838, 1120, 1249
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 5 cut(s) 91, 473, 1232, 1328, 1382
BsoBI CYCGRG 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 1158, 1389
Bsp143I GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
BspANI GGCC 5 cut(s) 91, 473, 1232, 1328, 1382
BspCNI CTCAG 3 cut(s) 931, 1209, 1305
BspFNI CGCG 4 cut(s) 470, 491, 808, 1068
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 6 cut(s) 309, 315, 364, 611, 1032, 1381
BspMI ACCTGC 2 cut(s) 337, 1237
BspPI GGATC 2 cut(s) 308, 321
BspT104I TTCGAA 1 cut(s) 330
BspT107I GGYRCC 1 cut(s) 609
BsrBI CCGCTC 1 cut(s) 162
BsrFI RCCGGY 3 cut(s) 501, 540, 832
BsrI ACTGG 5 cut(s) 183, 229, 739, 1286, 1521
BssAI RCCGGY 3 cut(s) 501, 540, 832
BssECI CCNNGG 4 cut(s) 474, 568, 1091, 1329
BssMI GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
BssNI GRCGYC 2 cut(s) 219, 294
BssT1I CCWWGG 1 cut(s) 1329
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 4 cut(s) 430, 796, 1111, 1340
Bst6I CTCTTC 3 cut(s) 517, 1165, 1521
BstACI GRCGYC 2 cut(s) 219, 294
BstAPI GCANNNNNTGC 1 cut(s) 234
BstBAI YACGTR 2 cut(s) 948, 1465
BstBI TTCGAA 1 cut(s) 330
BstC8I GCNNGC 4 cut(s) 830, 834, 1070, 1498
BstDEI CTNAG 3 cut(s) 918, 1196, 1313
BstDSI CCRYGG 2 cut(s) 568, 1091
BstF5I GGATG 3 cut(s) 192, 678, 895
BstFNI CGCG 4 cut(s) 470, 491, 808, 1068
BstKTI GATC 6 cut(s) 316, 801, 945, 976, 1054, 1553
BstMAI GTCTC 2 cut(s) 893, 1215
BstMBI GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
BstMCI CGRYCG 3 cut(s) 339, 502, 1054
BstMWI GCNNNNNNNGC 3 cut(s) 225, 234, 579
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 2 cut(s) 709, 832
BstUI CGCG 4 cut(s) 470, 491, 808, 1068
BstV1I GCAGC 4 cut(s) 127, 130, 355, 1422
BstV2I GAAGAC 1 cut(s) 1341
BstX2I RGATCY 2 cut(s) 313, 1550
BstYI RGATCY 2 cut(s) 313, 1550
BsuRI GGCC 5 cut(s) 91, 473, 1232, 1328, 1382
BtgI CCRYGG 2 cut(s) 568, 1091
BtrI CACGTC 1 cut(s) 586
BtsCI GGATG 3 cut(s) 192, 678, 895
BtsIMutI CAGTG 3 cut(s) 222, 926, 1293
BveI ACCTGC 2 cut(s) 337, 1237
Cac8I GCNNGC 4 cut(s) 830, 834, 1070, 1498
CaiI CAGNNNCTG 1 cut(s) 880
CciI TCATGA 1 cut(s) 432
Cfr10I RCCGGY 3 cut(s) 501, 540, 832
CpoI CGGWCCG 2 cut(s) 524, 1025
CseI GACGC 3 cut(s) 208, 302, 1232
Csp6I GTAC 5 cut(s) 610, 721, 742, 949, 1462
CspAI ACCGGT 1 cut(s) 501
CspI CGGWCCG 2 cut(s) 524, 1025
CviQI GTAC 5 cut(s) 610, 721, 742, 949, 1462
DdeI CTNAG 3 cut(s) 918, 1196, 1313
DpnI GATC 6 cut(s) 315, 800, 944, 975, 1053, 1552
DpnII GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
DriI GACNNNNNGTC 1 cut(s) 850
EaeI YGGCCR 1 cut(s) 89
Eam1104I CTCTTC 3 cut(s) 517, 1165, 1521
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 3 cut(s) 517, 1165, 1521
Eco130I CCWWGG 1 cut(s) 1329
Eco47I GGWCC 7 cut(s) 307, 524, 637, 1025, 1121, 1130, 1569
Eco57I CTGAAG 2 cut(s) 427, 737
Eco88I CYCGRG 1 cut(s) 784
EcoO109I RGGNCCY 2 cut(s) 1130, 1569
EcoRI GAATTC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 1329
ErhI CCWWGG 1 cut(s) 1329
Esp3I CGTCTC 2 cut(s) 893, 1215
FaqI GGGAC 4 cut(s) 394, 838, 1120, 1249
FauI CCCGC 2 cut(s) 265, 1177
FbaI TGATCA 1 cut(s) 942
FblI GTMKAC 3 cut(s) 465, 552, 666
Fnu4HI GCNGC 6 cut(s) 116, 119, 344, 471, 964, 1411
FokI GGATG 3 cut(s) 199, 665, 902
Fsp4HI GCNGC 6 cut(s) 116, 119, 344, 471, 964, 1411
FspBI CTAG 1 cut(s) 1566
GluI GCNGC 6 cut(s) 116, 119, 344, 471, 964, 1411
GsaI CCCAGC 2 cut(s) 115, 209
HaeIII GGCC 5 cut(s) 91, 473, 1232, 1328, 1382
HgaI GACGC 3 cut(s) 208, 302, 1232
Hin1I GRCGYC 2 cut(s) 219, 294
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 6 cut(s) 452, 529, 632, 913, 985, 1529
HpaI GTTAAC 1 cut(s) 202
HphI GGTGA 2 cut(s) 808, 952
Hpy166II GTNNAC 6 cut(s) 202, 307, 466, 553, 667, 895
Hpy188III TCNNGA 8 cut(s) 433, 589, 673, 682, 757, 784, 982, 1405
Hpy8I GTNNAC 6 cut(s) 202, 307, 466, 553, 667, 895
Hpy99I CGWCG 7 cut(s) 143, 293, 296, 392, 845, 956, 1078
HpyAV CCTTC 6 cut(s) 58, 175, 381, 766, 1044, 1096
HpyCH4III ACNGT 4 cut(s) 430, 796, 1111, 1340
HpyCH4IV ACGT 5 cut(s) 585, 902, 947, 1302, 1464
HpyCH4V TGCA 3 cut(s) 237, 1372, 1475
HpyF10VI GCNNNNNNNGC 3 cut(s) 225, 234, 579
HpyF3I CTNAG 3 cut(s) 918, 1196, 1313
HpySE526I ACGT 5 cut(s) 585, 902, 947, 1302, 1464
Hsp92I GRCGYC 2 cut(s) 219, 294
KpnI GGTACC 1 cut(s) 613
KroI GCCGGC 1 cut(s) 832
KroNI GCCGGC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 942
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
LmnI GCTCC 5 cut(s) 5, 167, 786, 1036, 1520
Lsp1109I GCAGC 4 cut(s) 127, 130, 355, 1422
LweI GCATC 1 cut(s) 754
MaeI CTAG 1 cut(s) 1566
MaeII ACGT 5 cut(s) 585, 902, 947, 1302, 1464
MaeIII GTNAC 4 cut(s) 220, 320, 809, 1288
MalI GATC 6 cut(s) 315, 800, 944, 975, 1053, 1552
MbiI CCGCTC 1 cut(s) 162
MboI GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
MboII GAAGA 8 cut(s) 504, 619, 1152, 1257, 1346, 1447, 1538, 1545
MfeI CAATTG 1 cut(s) 1536
MflI RGATCY 2 cut(s) 313, 1550
MhlI GDGCHC 2 cut(s) 1158, 1389
MluCI AATT 7 cut(s) 485, 516, 596, 1259, 1342, 1536, 1585
MlyI GAGTC 4 cut(s) 523, 922, 979, 1538
MmeI TCCRAC 4 cut(s) 164, 312, 551, 633
MroNI GCCGGC 1 cut(s) 832
MroXI GAANNNNTTC 3 cut(s) 440, 456, 1187
MseI TTAA 8 cut(s) 201, 885, 996, 1044, 1098, 1211, 1581, 1588
MunI CAATTG 1 cut(s) 1536
Mva1269I GAATGC 1 cut(s) 239
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 4 cut(s) 470, 491, 808, 1068
MwoI GCNNNNNNNGC 3 cut(s) 225, 234, 579
NaeI GCCGGC 1 cut(s) 834
NdeII GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
NgoMIV GCCGGC 1 cut(s) 832
NlaIV GGNNCC 6 cut(s) 309, 315, 364, 611, 1032, 1381
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 3 cut(s) 220, 320, 1288
NspI RCATGY 2 cut(s) 709, 832
NspV TTCGAA 1 cut(s) 330
PaeI GCATGC 1 cut(s) 832
PagI TCATGA 1 cut(s) 432
PcsI WCGNNNNNNNCGW 5 cut(s) 300, 336, 495, 908, 1065
PctI GAATGC 1 cut(s) 239
PdiI GCCGGC 1 cut(s) 834
PdmI GAANNNNTTC 3 cut(s) 440, 456, 1187
PfeI GAWTC 2 cut(s) 452, 632
Pfl23II CGTACG 2 cut(s) 948, 1461
PflMI CCANNNNNTGG 1 cut(s) 178
PinAI ACCGGT 1 cut(s) 501
PkrI GCNGC 6 cut(s) 117, 120, 345, 472, 965, 1412
Ple19I CGATCG 1 cut(s) 1054
PleI GAGTC 4 cut(s) 523, 921, 979, 1537
PpsI GAGTC 4 cut(s) 523, 921, 979, 1537
Ppu21I YACGTR 2 cut(s) 948, 1465
PpuMI RGGWCCY 2 cut(s) 1130, 1569
Psp5II RGGWCCY 2 cut(s) 1130, 1569
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 2 cut(s) 111, 205
PspGI CCWGG 1 cut(s) 474
PspLI CGTACG 2 cut(s) 948, 1461
PspN4I GGNNCC 6 cut(s) 309, 315, 364, 611, 1032, 1381
PspPPI RGGWCCY 2 cut(s) 1130, 1569
PstNI CAGNNNCTG 1 cut(s) 880
PsuI RGATCY 2 cut(s) 313, 1550
PvuI CGATCG 1 cut(s) 1054
RsaI GTAC 5 cut(s) 611, 722, 743, 950, 1463
RsaNI GTAC 5 cut(s) 610, 721, 742, 949, 1462
Rsr2I CGGWCCG 2 cut(s) 524, 1025
RsrII CGGWCCG 2 cut(s) 524, 1025
SaqAI TTAA 8 cut(s) 201, 885, 996, 1044, 1098, 1211, 1581, 1588
SatI GCNGC 6 cut(s) 116, 119, 344, 471, 964, 1411
Sau3AI GATC 6 cut(s) 313, 798, 942, 973, 1051, 1550
SchI GAGTC 4 cut(s) 523, 922, 979, 1538
SduI GDGCHC 2 cut(s) 1158, 1389
SfaNI GCATC 1 cut(s) 754
SfuI TTCGAA 1 cut(s) 330
SinI GGWCC 7 cut(s) 307, 524, 637, 1025, 1121, 1130, 1569
SmlI CTYRAG 3 cut(s) 821, 1125, 1405
SmoI CTYRAG 3 cut(s) 821, 1125, 1405
SphI GCATGC 1 cut(s) 832
Sse9I AATT 7 cut(s) 485, 516, 596, 1259, 1342, 1536, 1585
SspI AATATT 1 cut(s) 1579
SspMI CTAG 1 cut(s) 1566
StyI CCWWGG 1 cut(s) 1329
TaaI ACNGT 4 cut(s) 430, 796, 1111, 1340
TaiI ACGT 5 cut(s) 588, 905, 950, 1305, 1467
TaqII GACCGA 1 cut(s) 625
TasI AATT 7 cut(s) 485, 516, 596, 1259, 1342, 1536, 1585
TatI WGTACW 1 cut(s) 720
TauI GCSGC 2 cut(s) 473, 966
TfiI GAWTC 2 cut(s) 452, 632
Tru1I TTAA 8 cut(s) 201, 885, 996, 1044, 1098, 1211, 1581, 1588
Tru9I TTAA 8 cut(s) 201, 885, 996, 1044, 1098, 1211, 1581, 1588
TscAI CASTG 3 cut(s) 229, 926, 1293
TseFI GTSAC 3 cut(s) 220, 320, 1288
TseI GCWGC 4 cut(s) 115, 118, 343, 1410
Tsp45I GTSAC 3 cut(s) 220, 320, 1288
TspDTI ATGAA 4 cut(s) 203, 449, 657, 1477
TspGWI ACGGA 5 cut(s) 187, 445, 885, 978, 1337
TspRI CASTG 3 cut(s) 229, 926, 1293
Van91I CCANNNNNTGG 1 cut(s) 178
VpaK11BI GGWCC 7 cut(s) 307, 524, 637, 1025, 1121, 1130, 1569
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 709, 832
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 3 cut(s) 465, 552, 666
XmnI GAANNNNTTC 3 cut(s) 440, 456, 1187
XspI CTAG 1 cut(s) 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.