Rorug06G0501200

Non-specific phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011304
Physical Location & Seq
Reverse (-)
61626377 .. 61626847
471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0501200.1

Sequence Viewer

Length: 471 bp
ATGGATTTCAGAATCATGGGTCTGGATGCTCCAATCTTAACAGCACTGCAGCAGCTGGCGGACCTCCCGGAGGAGACCGAGAAGACCTTCAACGCCCCGACCCGGACCTACGTCCGCGACGCCAAGGCCATGGCTGCCACTCCGGCCGACGTGAAGGAGTTGCCCAATTCCTATGTCTTCGTCATCGACATGCCGGGACTCAAGTCAGGGGACATCAAGGTGCAGGTGGAGGATGACAATGTGCTTCTGATCAGCGGCGAGAGGAAGAGGGAGGAGGAGAAAGAGGGGGCTAAGTATGTGAGGATGGAGAGGAGGGTCGGTAAGTTTATGAGGAAGTTTGTGCTGCCTGAGAATGCTAATGCTGATACCATTTCTGCTGTTTGCCAAGATGGGGTTCTGACTGTGACTGTGGAGAAGCTGCCACCTCCTCAGCCGAAGAAGCCCAAGACTATTGAGGTCAAGATTGGTTAG

Protein Analysis

156

Amino Acids

17.4

Weight (kDa)

5.68

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 50 - 154 1.1e-31 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011304)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48610
fragaria_vesca FvH4_5g08480 FvH4_5g08480 FvH4_5g08480
malus_domestica MD06G1140600.v1.1 MD14G1156100.v1.1
prunus_persica Prupe.5G149800_v2.0.a1
pyrus_communis pycom06g13060 pycom14g12960
rosa_chinensis RchiOBHm_Chr7g0189931
rosa_laevigata RLG00000004576
rosa_multiflora Rmu_sc0014588.1_g000003 Rmu_sc0033399.1_g000001
rosa_roxburghii Rroxscaffold_3G00265040
rosa_rugosa Rorug06G0501200
rosa_samantha Rh7AG106300 Rh7BG109000 Rh7CG111100 Rh7DG109500
rosa_wichuraiana Rw7G009230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 214
AccII CGCG 1 cut(s) 117
AciI CCGC 3 cut(s) 59, 115, 255
AcoI YGGCCR 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 70, 102, 391
AgsI TTSAA 1 cut(s) 91
AjiI CACGTC 1 cut(s) 151
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
AluBI AGCT 2 cut(s) 55, 418
AluI AGCT 2 cut(s) 55, 418
Alw26I GTCTC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 55
AoxI GGCC 2 cut(s) 126, 144
ApeKI GCWGC 5 cut(s) 49, 52, 134, 343, 418
Asp700I GAANNNNTTC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 61, 105
AsuC2I CCSGG 3 cut(s) 68, 103, 195
AvaII GGWCC 2 cut(s) 61, 105
BbsI GAAGAC 2 cut(s) 89, 169
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 5 cut(s) 61, 64, 121, 330, 405
BccI CCATC 2 cut(s) 298, 383
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 3 cut(s) 68, 103, 195
BcoDI GTCTC 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 6 cut(s) 50, 53, 135, 256, 344, 419
BlsI GCNGC 6 cut(s) 51, 54, 136, 257, 345, 420
Bme1390I CCNGG 3 cut(s) 68, 103, 195
Bme18I GGWCC 2 cut(s) 61, 105
BmgBI CACGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 61, 105
BmrFI CCNGG 3 cut(s) 68, 103, 195
BmsI GCATC 1 cut(s) 16
BoxI GACNNNNGTC 2 cut(s) 110, 202
BpiI GAAGAC 2 cut(s) 89, 169
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 3 cut(s) 68, 103, 195
BsaHI GRCGYC 1 cut(s) 120
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 123, 129
BsaXI ACNNNNNCTCC 2 cut(s) 304, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 102, 391
BseDI CCNNGG 2 cut(s) 123, 129
BseGI GGATG 3 cut(s) 31, 238, 309
BseLI CCNNNNNNNGG 3 cut(s) 70, 102, 391
BseMII CTCAG 2 cut(s) 339, 443
BseRI GAGGAG 5 cut(s) 86, 287, 290, 325, 417
BseX3I CGGCCG 1 cut(s) 144
BseXI GCAGC 5 cut(s) 61, 64, 121, 330, 405
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 147
BshFI GGCC 2 cut(s) 128, 146
BsiEI CGRYCG 1 cut(s) 147
BsiSI CCGG 4 cut(s) 68, 103, 143, 194
BslFI GGGAC 2 cut(s) 210, 224
BslI CCNNNNNNNGG 3 cut(s) 70, 102, 391
BsmAI GTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 210, 224
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 2 cut(s) 128, 146
Bso31I GGTCTC 1 cut(s) 68
Bsp143I GATC 1 cut(s) 249
Bsp19I CCATGG 1 cut(s) 129
BspACI CCGC 3 cut(s) 59, 115, 255
BspANI GGCC 2 cut(s) 128, 146
BspCNI CTCAG 2 cut(s) 340, 442
BspFNI CGCG 1 cut(s) 117
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 123, 129
BssMI GATC 1 cut(s) 249
BssNI GRCGYC 1 cut(s) 120
BssT1I CCWWGG 2 cut(s) 123, 129
Bst4CI ACNGT 2 cut(s) 403, 409
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 57
BstDEI CTNAG 3 cut(s) 291, 348, 429
BstDSI CCRYGG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 68
BstF5I GGATG 3 cut(s) 31, 238, 309
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 1 cut(s) 252
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 1 cut(s) 249
BstMCI CGRYCG 1 cut(s) 147
BstMWI GCNNNNNNNGC 3 cut(s) 134, 143, 439
BstNSI RCATGY 1 cut(s) 193
BstPAI GACNNNNGTC 2 cut(s) 110, 202
BstSCI CCNGG 3 cut(s) 66, 101, 193
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 5 cut(s) 61, 64, 121, 330, 405
BstV2I GAAGAC 2 cut(s) 89, 169
BstXI CCANNNNNNTGG 1 cut(s) 130
BstZI CGGCCG 1 cut(s) 144
BsuRI GGCC 2 cut(s) 128, 146
BtgI CCRYGG 1 cut(s) 129
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 3 cut(s) 31, 238, 309
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 44
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 1 cut(s) 57
CaiI CAGNNNCTG 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 61, 105
CseI GACGC 1 cut(s) 128
CviAII CATG 3 cut(s) 16, 130, 190
CviJI RGCY 8 cut(s) 55, 128, 134, 146, 290, 418, 433, 442
CviKI_1 RGCY 8 cut(s) 55, 128, 134, 146, 290, 418, 433, 442
DdeI CTNAG 3 cut(s) 291, 348, 429
DpnI GATC 1 cut(s) 251
DpnII GATC 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 144
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
EciI GGCGGA 1 cut(s) 74
EclXI CGGCCG 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 123, 129
Eco31I GGTCTC 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 61, 105
Eco52I CGGCCG 1 cut(s) 144
EcoNI CCTNNNNNAGG 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 123, 129
ErhI CCWWGG 2 cut(s) 123, 129
FaeI CATG 3 cut(s) 19, 133, 193
FaiI YATR 6 cut(s) 17, 131, 174, 191, 297, 329
FaqI GGGAC 2 cut(s) 210, 224
FatI CATG 3 cut(s) 15, 129, 189
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 6 cut(s) 50, 53, 135, 256, 344, 419
FokI GGATG 3 cut(s) 38, 245, 316
Fsp4HI GCNGC 6 cut(s) 50, 53, 135, 256, 344, 419
GluI GCNGC 6 cut(s) 50, 53, 135, 256, 344, 419
HaeIII GGCC 2 cut(s) 128, 146
HapII CCGG 4 cut(s) 68, 103, 143, 194
HgaI GACGC 1 cut(s) 128
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 3 cut(s) 19, 133, 193
HinfI GANTC 2 cut(s) 12, 198
HpaII CCGG 4 cut(s) 68, 103, 143, 194
Hpy188I TCNGA 3 cut(s) 11, 249, 399
Hpy188III TCNNGA 2 cut(s) 23, 460
Hpy99I CGWCG 2 cut(s) 122, 152
HpyAV CCTTC 2 cut(s) 97, 148
HpyCH4III ACNGT 2 cut(s) 403, 409
HpyCH4IV ACGT 2 cut(s) 111, 150
HpyCH4V TGCA 2 cut(s) 49, 223
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 143, 439
HpyF3I CTNAG 3 cut(s) 291, 348, 429
HpySE526I ACGT 2 cut(s) 111, 150
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 3 cut(s) 19, 133, 193
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 1 cut(s) 249
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 9 cut(s) 8, 41, 81, 116, 156, 192, 207, 209, 360
Lsp1109I GCAGC 5 cut(s) 61, 64, 121, 330, 405
LweI GCATC 1 cut(s) 16
MaeII ACGT 2 cut(s) 111, 150
MaeIII GTNAC 1 cut(s) 403
MalI GATC 1 cut(s) 251
MboI GATC 1 cut(s) 249
MboII GAAGA 4 cut(s) 94, 169, 277, 448
MluCI AATT 1 cut(s) 166
MlyI GAGTC 1 cut(s) 192
MroXI GAANNNNTTC 1 cut(s) 86
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 2 cut(s) 188, 218
MspA1I CMGCKG 2 cut(s) 55, 255
MspI CCGG 4 cut(s) 68, 103, 143, 194
MspR9I CCNGG 3 cut(s) 68, 103, 195
Mva1269I GAATGC 1 cut(s) 358
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 3 cut(s) 134, 143, 439
NciI CCSGG 3 cut(s) 68, 103, 195
NcoI CCATGG 1 cut(s) 129
NdeII GATC 1 cut(s) 249
NlaIII CATG 3 cut(s) 19, 133, 193
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 1 cut(s) 193
PaqCI CACCTGC 1 cut(s) 214
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 66
PkrI GCNGC 6 cut(s) 51, 54, 136, 257, 345, 420
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
PshAI GACNNNNGTC 2 cut(s) 110, 202
PspPI GGNCC 2 cut(s) 61, 105
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 55
PvuII CAGCTG 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 188, 218
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 6 cut(s) 50, 53, 135, 256, 344, 419
Sau3AI GATC 1 cut(s) 249
Sau96I GGNCC 2 cut(s) 61, 105
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 3 cut(s) 68, 103, 195
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 47
SinI GGWCC 2 cut(s) 61, 105
SmiMI CAYNNNNRTG 2 cut(s) 188, 218
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 1 cut(s) 166
SsiI CCGC 3 cut(s) 59, 115, 255
StyD4I CCNGG 3 cut(s) 66, 101, 193
StyI CCWWGG 2 cut(s) 123, 129
TaaI ACNGT 2 cut(s) 403, 409
TaiI ACGT 2 cut(s) 114, 153
TaqI TCGA 1 cut(s) 186
TaqII GACCGA 1 cut(s) 92
TasI AATT 1 cut(s) 166
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 5 cut(s) 49, 52, 134, 343, 418
Tsp45I GTSAC 1 cut(s) 403
TspRI CASTG 1 cut(s) 51
VpaK11BI GGWCC 2 cut(s) 61, 105
XagI CCTNNNNNAGG 1 cut(s) 68
XceI RCATGY 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.