Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0009901

Physical Location & Seq

Genomic Coordinates Forward (+)

18292590 .. 18294743

Gene Structure 2154 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g13270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGCAACTTTCATCCTCAAAAACTCTCCATTTGCAAACCGAAATTTTCTCTTCAAAGGCAGCCATGTTTGTAGCTCTCCTTTTACCTTGGTCCCTTTTGTTCCTCCTCTATGTCTTCTTCACCACCATCCAGAAATATCAAACAAAACTACCGAAACCACCATTGAGTTCTACCTATGGGCCTCCTTCCTACCCCATAATTGGCTGCTTTATCTCTTTCTGCAAGAACCAACACAACCTCCTAGACTGGTACACCAGTCTCCTCTCAGCCTCACCGTCACAGACTATAGTTGTGCACAGGATAGGTGCACGACGCATTGTTGTGACGGCAAACCCATCCAACGTGGAGCACATTCTCAAAACAAACTTTGTAAACTTCCCCAAGGGAAAACCCCTAACTGACTTCCTCGGGGACCTTCTGGGCTGCGGCATATTCAACGTGGATGGCGACTTGTGGTCCACGCAGCGGAAGCTCGCGAGCCATGAGTTCAGCACAAAGTCCCTGAGGGAGTTTGTTGTGAAGACTCTTGAGGAAGAAGTCGAGGGCCGACTTATTCCGCTGCTGGAAGAAGCTGCAGAGAGCCAAAGGGTTTTGGATATGCAGGAAATACTGAAGATGTTCGCCTTTGACACTGTGTGTAAGGTTTCATTGGGCACCGACCCATTTTGCTTGGATTTGAACCGACCCGTGCCTCCCCTTGTGAAAGCTTTTGATTGTGCTGCTGAGATAAGTGCCAGAAGGGCTACAGCTCCTTTGTATTTGATGTGGAAGATGAAGCGTGCTTTGAGCATTGGGTCCGAAAAGAAGCTCAGGGAAGCAGTGAAACTCGTTCATGGCTCTGTTCATGAAATAATCCAAAACAAGAAGAAGATTCTTCAAAGAGATGAAGGAAGGAGAATTGAAAGCCACGACATATTGTCACGGTTGTTACTAGCACGACACGGGGAGGAAGTGGTGAGAGACATGGTGATAAGTTTCATACTTGCCGGACGTGACACCACCTCAGCAGCCATGACTTGGCTCTTCTGGTTGCTCAGCAAGCATCCTGACATAACCCACAAGATTGTGAACGAAGTCGAAAATGTCGTCGTACAAAACAATGGTACGGAAAAACTAGGGTTTGATGATTTGCAAAAAATGAGGTTTCTGAGGGCTAGTTTGTGTGAGACTATGAGGCTCTACCCACCAGTCCCATGGGATTCCAAGCATGCTGAGGTGGACACTGTCTTGCCAGATGGGACTTGGGTTGGGAAGGGAGATAGGGTTACTTATTTTCCATATGGGATGGGGAGGATGGAGGAGCTGTGGGGAAAAGACCGGTTTGAGTTTAACCCGGACAGGTGGTTTGACATTGAACCGGGTTGTGATGGTGATAGGGTTTTGAAATTTGTGAGTCCATACAAGTTCCCGGTTTTTCAGGCCGGTCCAAGGGTTTGTCTTGGGAAGGAGATGGCTTTTATTCAGATGAAGTATGTGATGGCTTCGGTGTTGAGGCGGTTTGAGATCAGACCGGTTTGTGGGGAAGACCGGCCGGCTTTTGTTCCCCTCTTGACGGCTCATATGGCAGGTGGGTTGAAGGTGTTGGTTCGAAAAAGAAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0002213 GO:0003006 GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006950 GO:0006952 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009611 GO:0009694 GO:0009791 GO:0009908 GO:0009987 GO:0010154 GO:0010817 GO:0012505 GO:0016020 GO:0016491 GO:0019752 GO:0022414 GO:0031984 GO:0032501 GO:0032502 GO:0032787 GO:0042175 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048443 GO:0048466 GO:0048467 GO:0048480 GO:0048608 GO:0048653 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0052694 GO:0055114 GO:0061458 GO:0065007 GO:0065008 GO:0071704 GO:0090567 GO:0098827 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

p450

Protein Analysis

534

Amino Acids

60.69

Weight (kDa)

9.05

Isoelectric Point (pI)

42.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	60 - 511	2.9e-67	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009901)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48520 AT5G63450 AT5G63450
fragaria_vesca	FvH4_5g08100 FvH4_5g08170
malus_domestica	MD06G1142900.v1.1 MD14G1158100.v1.1
prunus_persica	Prupe.5G152200_v2.0.a1
pyrus_communis	pycom06g13270 pycom14g13170
rosa_chinensis	RchiOBHm_Chr7g0190341
rosa_laevigata	RLG00000004540
rosa_multiflora	Rmu_co8407537.1_g000001 Rmu_ssc0000038.1_g000004
rosa_roxburghii	Rroxscaffold_3G00264710
rosa_rugosa	Rorug06G0504000
rosa_samantha	Rh7AG109700 Rh7BG112300 Rh7CG114600 Rh7DG112700
rosa_wichuraiana	Rw7G009520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1556
Acc36I	ACCTGC	1 cut(s)	1556
AccB1I	GGYRCC	1 cut(s)	653
AccB7I	CCANNNNNTGG	1 cut(s)	1017
AccII	CGCG	1 cut(s)	476
AciI	CCGC	4 cut(s)	426, 466, 557, 1495
AcoI	YGGCCR	1 cut(s)	1529
AcsI	RAATTY	2 cut(s)	42, 1385
AcuI	CTGAAG	1 cut(s)	632
AdeI	CACNNNGTG	1 cut(s)	636
AfaI	GTAC	3 cut(s)	251, 1092, 1105
AfiI	CCNNNNNNNGG	6 cut(s)	200, 465, 1017, 1428, 1517, 1552
AgeI	ACCGGT	2 cut(s)	1317, 1510
AgsI	TTSAA	8 cut(s)	54, 436, 679, 878, 902, 1355, 1384, 1576
AhdI	GACNNNNNGTC	2 cut(s)	454, 916
AjiI	CACGTC	1 cut(s)	992
AluBI	AGCT	7 cut(s)	74, 472, 572, 707, 749, 808, 1303
AluI	AGCT	7 cut(s)	74, 472, 572, 707, 749, 808, 1303
Alw21I	GWGCWC	3 cut(s)	297, 310, 351
Alw26I	GTCTC	3 cut(s)	263, 954, 1160
Alw44I	GTGCAC	2 cut(s)	293, 306
Ama87I	CYCGRG	1 cut(s)	407
AoxI	GGCC	4 cut(s)	179, 544, 1419, 1529
ApaLI	GTGCAC	2 cut(s)	293, 306
ApeKI	GCWGC	8 cut(s)	59, 204, 423, 463, 559, 572, 719, 1007
ApoI	RAATTY	2 cut(s)	42, 1385
ArsI	GACNNNNNNTTYG	4 cut(s)	1071, 1103, 1328, 1360
AsiGI	ACCGGT	2 cut(s)	1317, 1510
Asp700I	GAANNNNTTC	1 cut(s)	617
AspS9I	GGNCC	7 cut(s)	90, 179, 412, 456, 544, 795, 1424
AsuC2I	CCSGG	3 cut(s)	1334, 1359, 1409
AsuHPI	GGTGA	5 cut(s)	112, 264, 967, 979, 1382
AsuII	TTCGAA	1 cut(s)	1588
AvaI	CYCGRG	1 cut(s)	407
AvaII	GGWCC	5 cut(s)	90, 412, 456, 795, 1424
AxyI	CCTNAGG	1 cut(s)	503
BaeGI	GKGCMC	3 cut(s)	297, 310, 656
BaeI	ACNNNNGTAYC	2 cut(s)	241, 274
BanI	GGYRCC	1 cut(s)	653
BbsI	GAAGAC	3 cut(s)	106, 527, 1530
Bbv12I	GWGCWC	3 cut(s)	297, 310, 351
BbvCI	CCTCAGC	2 cut(s)	1003, 1212
BbvI	GCAGC	8 cut(s)	71, 191, 410, 475, 546, 559, 706, 1019
BccI	CCATC	9 cut(s)	134, 343, 437, 1229, 1279, 1288, 1361, 1444, 1471
BceAI	ACGGC	2 cut(s)	342, 1569
BcnI	CCSGG	3 cut(s)	1334, 1359, 1409
BcoDI	GTCTC	3 cut(s)	263, 954, 1160
BfaI	CTAG	4 cut(s)	242, 932, 1115, 1155
BfmI	CTRYAG	3 cut(s)	285, 573, 744
BfuAI	ACCTGC	1 cut(s)	1556
BglI	GCCNNNNNGGC	1 cut(s)	740
BisI	GCNGC	9 cut(s)	60, 205, 424, 427, 464, 560, 573, 720, 1008
BlpI	GCTNAGC	1 cut(s)	1034
BlsI	GCNGC	9 cut(s)	61, 206, 425, 428, 465, 561, 574, 721, 1009
Bme1390I	CCNGG	3 cut(s)	1334, 1359, 1409
Bme18I	GGWCC	5 cut(s)	90, 412, 456, 795, 1424
BmeRI	GACNNNNNGTC	2 cut(s)	454, 916
BmeT110I	CYCGRG	1 cut(s)	407
BmgBI	CACGTC	1 cut(s)	992
BmgT120I	GGNCC	7 cut(s)	90, 179, 412, 456, 544, 795, 1424
BmiI	GGNNCC	4 cut(s)	92, 413, 655, 796
BmrFI	CCNGG	3 cut(s)	1334, 1359, 1409
BmsI	GCATC	1 cut(s)	1051
BpiI	GAAGAC	3 cut(s)	106, 527, 1530
Bpu10I	CCTNAGC	3 cut(s)	809, 1003, 1212
Bpu1102I	GCTNAGC	1 cut(s)	1034
Bpu14I	TTCGAA	1 cut(s)	1588
BpuEI	CTTGAG	1 cut(s)	548
BpuMI	CCSGG	3 cut(s)	1334, 1359, 1409
BsaJI	CCNNGG	5 cut(s)	86, 381, 406, 1193, 1427
BsaWI	WCCGGW	2 cut(s)	1317, 1510
BsaXI	ACNNNNNCTCC	6 cut(s)	222, 252, 1248, 1278, 1289, 1319
Bsc4I	CCNNNNNNNGG	6 cut(s)	200, 465, 1017, 1428, 1517, 1552
Bse118I	RCCGGY	5 cut(s)	1317, 1421, 1510, 1527, 1531
Bse1I	ACTGG	3 cut(s)	251, 255, 1187
Bse21I	CCTNAGG	1 cut(s)	503
BseDI	CCNNGG	5 cut(s)	86, 381, 406, 1193, 1427
BseGI	GGATG	7 cut(s)	11, 126, 335, 448, 1042, 1290, 1299
BseLI	CCNNNNNNNGG	6 cut(s)	200, 465, 1017, 1428, 1517, 1552
BseMII	CTCAG	8 cut(s)	279, 494, 714, 823, 1017, 1048, 1139, 1203
BseNI	ACTGG	3 cut(s)	251, 255, 1187
BseRI	GAGGAG	3 cut(s)	95, 251, 1313
BseSI	GKGCMC	3 cut(s)	297, 310, 656
BseX3I	CGGCCG	1 cut(s)	1529
BseXI	GCAGC	8 cut(s)	71, 191, 410, 475, 546, 559, 706, 1019
Bsh1236I	CGCG	1 cut(s)	476
Bsh1285I	CGRYCG	1 cut(s)	1532
BshFI	GGCC	4 cut(s)	181, 546, 1421, 1531
BshNI	GGYRCC	1 cut(s)	653
BshTI	ACCGGT	2 cut(s)	1317, 1510
BsiEI	CGRYCG	1 cut(s)	1532
BsiHKAI	GWGCWC	3 cut(s)	297, 310, 351
BsiHKCI	CYCGRG	1 cut(s)	407
BsiSI	CCGG	9 cut(s)	987, 1318, 1334, 1358, 1409, 1422, 1511, 1528, 1532
BslFI	GGGAC	5 cut(s)	76, 425, 484, 1175, 1252
BslI	CCNNNNNNNGG	6 cut(s)	200, 465, 1017, 1428, 1517, 1552
BsmAI	GTCTC	3 cut(s)	263, 954, 1160
BsmFI	GGGAC	5 cut(s)	76, 425, 484, 1175, 1252
BsnI	GGCC	4 cut(s)	181, 546, 1421, 1531
BsoBI	CYCGRG	1 cut(s)	407
Bsp119I	TTCGAA	1 cut(s)	1588
Bsp1286I	GDGCHC	4 cut(s)	297, 310, 351, 656
Bsp143I	GATC	1 cut(s)	1503
Bsp1720I	GCTNAGC	1 cut(s)	1034
Bsp19I	CCATGG	1 cut(s)	1193
Bsp68I	TCGCGA	1 cut(s)	476
BspACI	CCGC	4 cut(s)	426, 466, 557, 1495
BspANI	GGCC	4 cut(s)	181, 546, 1421, 1531
BspCNI	CTCAG	8 cut(s)	278, 495, 715, 822, 1016, 1047, 1140, 1204
BspFNI	CGCG	1 cut(s)	476
BspHI	TCATGA	1 cut(s)	844
BspLI	GGNNCC	4 cut(s)	92, 413, 655, 796
BspMAI	CTGCAG	1 cut(s)	577
BspMI	ACCTGC	1 cut(s)	1556
BspQI	GCTCTTC	1 cut(s)	1028
BspT104I	TTCGAA	1 cut(s)	1588
BspT107I	GGYRCC	1 cut(s)	653
BsrFI	RCCGGY	5 cut(s)	1317, 1421, 1510, 1527, 1531
BsrI	ACTGG	3 cut(s)	251, 255, 1187
BssAI	RCCGGY	5 cut(s)	1317, 1421, 1510, 1527, 1531
BssECI	CCNNGG	5 cut(s)	86, 381, 406, 1193, 1427
BssMI	GATC	1 cut(s)	1503
BssT1I	CCWWGG	4 cut(s)	86, 381, 1193, 1427
Bst4CI	ACNGT	4 cut(s)	276, 634, 924, 1225
Bst6I	CTCTTC	2 cut(s)	55, 1028
BstBI	TTCGAA	1 cut(s)	1588
BstC8I	GCNNGC	6 cut(s)	474, 478, 780, 1040, 1209, 1533
BstDEI	CTNAG	8 cut(s)	265, 503, 723, 809, 1003, 1034, 1148, 1212
BstDSI	CCRYGG	1 cut(s)	1193
BstF5I	GGATG	7 cut(s)	11, 126, 335, 448, 1042, 1290, 1299
BstFNI	CGCG	1 cut(s)	476
BstKTI	GATC	1 cut(s)	1506
BstMAI	GTCTC	3 cut(s)	263, 954, 1160
BstMBI	GATC	1 cut(s)	1503
BstMCI	CGRYCG	1 cut(s)	1532
BstMWI	GCNNNNNNNGC	4 cut(s)	469, 740, 1039, 1562
BstNSI	RCATGY	1 cut(s)	1211
BstSCI	CCNGG	3 cut(s)	1332, 1357, 1407
BstSFI	CTRYAG	3 cut(s)	285, 573, 744
BstSLI	GKGCMC	3 cut(s)	297, 310, 656
BstUI	CGCG	1 cut(s)	476
BstV1I	GCAGC	8 cut(s)	71, 191, 410, 475, 546, 559, 706, 1019
BstV2I	GAAGAC	3 cut(s)	106, 527, 1530
BstXI	CCANNNNNNTGG	1 cut(s)	1194
BstZI	CGGCCG	1 cut(s)	1529
Bsu36I	CCTNAGG	1 cut(s)	503
BsuRI	GGCC	4 cut(s)	181, 546, 1421, 1531
BtgI	CCRYGG	1 cut(s)	1193
BtrI	CACGTC	1 cut(s)	992
BtsCI	GGATG	7 cut(s)	11, 126, 335, 448, 1042, 1290, 1299
BtsI	GCAGTG	1 cut(s)	825
BtsIMutI	CAGTG	3 cut(s)	630, 825, 1221
BtuMI	TCGCGA	1 cut(s)	476
BveI	ACCTGC	1 cut(s)	1556
Cac8I	GCNNGC	6 cut(s)	474, 478, 780, 1040, 1209, 1533
CciI	TCATGA	1 cut(s)	844
Cfr10I	RCCGGY	5 cut(s)	1317, 1421, 1510, 1527, 1531
Cfr13I	GGNCC	7 cut(s)	90, 179, 412, 456, 544, 795, 1424
CseI	GACGC	1 cut(s)	321
Csp6I	GTAC	3 cut(s)	250, 1091, 1104
CspAI	ACCGGT	2 cut(s)	1317, 1510
CviAII	CATG	8 cut(s)	64, 482, 833, 845, 964, 1012, 1194, 1208
CviQI	GTAC	3 cut(s)	250, 1091, 1104
DdeI	CTNAG	8 cut(s)	265, 503, 723, 809, 1003, 1034, 1148, 1212
DpnI	GATC	1 cut(s)	1505
DpnII	GATC	1 cut(s)	1503
DraIII	CACNNNGTG	1 cut(s)	636
DriI	GACNNNNNGTC	2 cut(s)	454, 916
EaeI	YGGCCR	1 cut(s)	1529
EagI	CGGCCG	1 cut(s)	1529
Eam1104I	CTCTTC	2 cut(s)	55, 1028
Eam1105I	GACNNNNNGTC	2 cut(s)	454, 916
EarI	CTCTTC	2 cut(s)	55, 1028
EclXI	CGGCCG	1 cut(s)	1529
Eco130I	CCWWGG	4 cut(s)	86, 381, 1193, 1427
Eco47I	GGWCC	5 cut(s)	90, 412, 456, 795, 1424
Eco52I	CGGCCG	1 cut(s)	1529
Eco57I	CTGAAG	1 cut(s)	632
Eco81I	CCTNAGG	1 cut(s)	503
Eco88I	CYCGRG	1 cut(s)	407
EcoO109I	RGGNCCY	1 cut(s)	412
EcoT14I	CCWWGG	4 cut(s)	86, 381, 1193, 1427
ErhI	CCWWGG	4 cut(s)	86, 381, 1193, 1427
FaeI	CATG	8 cut(s)	67, 485, 836, 848, 967, 1015, 1197, 1211
FaqI	GGGAC	5 cut(s)	76, 425, 484, 1175, 1252
FatI	CATG	8 cut(s)	63, 481, 832, 844, 963, 1011, 1193, 1207
FauNDI	CATATG	2 cut(s)	1279, 1560
Fnu4HI	GCNGC	9 cut(s)	60, 205, 424, 427, 464, 560, 573, 720, 1008
FokI	GGATG	6 cut(s)	113, 322, 455, 1029, 1297, 1306
Fsp4HI	GCNGC	9 cut(s)	60, 205, 424, 427, 464, 560, 573, 720, 1008
FspBI	CTAG	4 cut(s)	242, 932, 1115, 1155
GluI	GCNGC	9 cut(s)	60, 205, 424, 427, 464, 560, 573, 720, 1008
HaeIII	GGCC	4 cut(s)	181, 546, 1421, 1531
HapII	CCGG	9 cut(s)	987, 1318, 1334, 1358, 1409, 1422, 1511, 1528, 1532
HgaI	GACGC	1 cut(s)	321
Hin1II	CATG	8 cut(s)	67, 485, 836, 848, 967, 1015, 1197, 1211
HindIII	AAGCTT	1 cut(s)	705
HinfI	GANTC	4 cut(s)	523, 871, 1199, 1393
HpaII	CCGG	9 cut(s)	987, 1318, 1334, 1358, 1409, 1422, 1511, 1528, 1532
HphI	GGTGA	5 cut(s)	112, 264, 967, 979, 1382
Hpy166II	GTNNAC	7 cut(s)	252, 295, 308, 373, 459, 1069, 1219
Hpy188I	TCNGA	4 cut(s)	799, 1149, 1464, 1508
Hpy188III	TCNNGA	6 cut(s)	130, 475, 527, 845, 1046, 1549
Hpy8I	GTNNAC	7 cut(s)	252, 295, 308, 373, 459, 1069, 1219
Hpy99I	CGWCG	2 cut(s)	315, 1091
HpyAV	CCTTC	8 cut(s)	195, 425, 732, 881, 885, 1246, 1438, 1570
HpyCH4III	ACNGT	4 cut(s)	276, 634, 924, 1225
HpyCH4IV	ACGT	3 cut(s)	342, 438, 991
HpyCH4V	TGCA	8 cut(s)	4, 34, 222, 295, 308, 575, 601, 1132
HpyF10VI	GCNNNNNNNGC	4 cut(s)	469, 740, 1039, 1562
HpyF3I	CTNAG	8 cut(s)	265, 503, 723, 809, 1003, 1034, 1148, 1212
HpySE526I	ACGT	3 cut(s)	342, 438, 991
Hsp92II	CATG	8 cut(s)	67, 485, 836, 848, 967, 1015, 1197, 1211
KroI	GCCGGC	1 cut(s)	1531
KroNI	GCCGGC	1 cut(s)	1533
Kzo9I	GATC	1 cut(s)	1503
LguI	GCTCTTC	1 cut(s)	1028
LmnI	GCTCC	3 cut(s)	346, 754, 1300
Lsp1109I	GCAGC	8 cut(s)	71, 191, 410, 475, 546, 559, 706, 1019
LweI	GCATC	1 cut(s)	1051
MaeI	CTAG	4 cut(s)	242, 932, 1115, 1155
MaeII	ACGT	3 cut(s)	342, 438, 991
MaeIII	GTNAC	6 cut(s)	276, 322, 918, 927, 992, 1264
MalI	GATC	1 cut(s)	1505
MboI	GATC	1 cut(s)	1503
MhlI	GDGCHC	4 cut(s)	297, 310, 351, 656
MluCI	AATT	4 cut(s)	42, 198, 897, 1385
MlyI	GAGTC	2 cut(s)	517, 1402
MmeI	TCCRAC	1 cut(s)	363
MroNI	GCCGGC	1 cut(s)	1531
MroXI	GAANNNNTTC	1 cut(s)	617
MseI	TTAA	1 cut(s)	1329
MslI	CAYNNNNRTG	1 cut(s)	320
MspA1I	CMGCKG	2 cut(s)	466, 559
MspI	CCGG	9 cut(s)	987, 1318, 1334, 1358, 1409, 1422, 1511, 1528, 1532
MspR9I	CCNGG	3 cut(s)	1334, 1359, 1409
MvnI	CGCG	1 cut(s)	476
MwoI	GCNNNNNNNGC	4 cut(s)	469, 740, 1039, 1562
NaeI	GCCGGC	1 cut(s)	1533
NciI	CCSGG	3 cut(s)	1334, 1359, 1409
NcoI	CCATGG	1 cut(s)	1193
NdeI	CATATG	2 cut(s)	1279, 1560
NdeII	GATC	1 cut(s)	1503
NgoMIV	GCCGGC	1 cut(s)	1531
NlaIII	CATG	8 cut(s)	67, 485, 836, 848, 967, 1015, 1197, 1211
NlaIV	GGNNCC	4 cut(s)	92, 413, 655, 796
NmuCI	GTSAC	4 cut(s)	276, 322, 918, 992
NruI	TCGCGA	1 cut(s)	476
NspI	RCATGY	1 cut(s)	1211
NspV	TTCGAA	1 cut(s)	1588
PaeI	GCATGC	1 cut(s)	1211
PagI	TCATGA	1 cut(s)	844
PaqCI	CACCTGC	1 cut(s)	1556
PciSI	GCTCTTC	1 cut(s)	1028
PcsI	WCGNNNNNNNCGW	2 cut(s)	444, 1083
PdiI	GCCGGC	1 cut(s)	1533
PdmI	GAANNNNTTC	1 cut(s)	617
PfeI	GAWTC	2 cut(s)	871, 1199
PflFI	GACNNNGTC	1 cut(s)	1223
PflMI	CCANNNNNTGG	1 cut(s)	1017
PinAI	ACCGGT	2 cut(s)	1317, 1510
PkrI	GCNGC	9 cut(s)	61, 206, 425, 428, 465, 561, 574, 721, 1009
PleI	GAGTC	2 cut(s)	517, 1401
PpsI	GAGTC	2 cut(s)	517, 1401
PpuMI	RGGWCCY	1 cut(s)	412
Psp5II	RGGWCCY	1 cut(s)	412
PspN4I	GGNNCC	4 cut(s)	92, 413, 655, 796
PspPI	GGNCC	7 cut(s)	90, 179, 412, 456, 544, 795, 1424
PspPPI	RGGWCCY	1 cut(s)	412
PstI	CTGCAG	1 cut(s)	577
PsyI	GACNNNGTC	1 cut(s)	1223
RruI	TCGCGA	1 cut(s)	476
RsaI	GTAC	3 cut(s)	251, 1092, 1105
RsaNI	GTAC	3 cut(s)	250, 1091, 1104
RseI	CAYNNNNRTG	1 cut(s)	320
SapI	GCTCTTC	1 cut(s)	1028
SaqAI	TTAA	1 cut(s)	1329
SatI	GCNGC	9 cut(s)	60, 205, 424, 427, 464, 560, 573, 720, 1008
Sau3AI	GATC	1 cut(s)	1503
Sau96I	GGNCC	7 cut(s)	90, 179, 412, 456, 544, 795, 1424
SchI	GAGTC	2 cut(s)	517, 1402
ScrFI	CCNGG	3 cut(s)	1334, 1359, 1409
SduI	GDGCHC	4 cut(s)	297, 310, 351, 656
SfaNI	GCATC	1 cut(s)	1051
SfcI	CTRYAG	3 cut(s)	285, 573, 744
SfuI	TTCGAA	1 cut(s)	1588
SinI	GGWCC	5 cut(s)	90, 412, 456, 795, 1424
SmiMI	CAYNNNNRTG	1 cut(s)	320
SmlI	CTYRAG	1 cut(s)	527
SmoI	CTYRAG	1 cut(s)	527
SphI	GCATGC	1 cut(s)	1211
Sse9I	AATT	4 cut(s)	42, 198, 897, 1385
SsiI	CCGC	4 cut(s)	426, 466, 557, 1495
SspMI	CTAG	4 cut(s)	242, 932, 1115, 1155
StyD4I	CCNGG	3 cut(s)	1332, 1357, 1407
StyI	CCWWGG	4 cut(s)	86, 381, 1193, 1427
TaaI	ACNGT	4 cut(s)	276, 634, 924, 1225
TaiI	ACGT	3 cut(s)	345, 441, 994
TaqI	TCGA	3 cut(s)	540, 1077, 1588
TasI	AATT	4 cut(s)	42, 198, 897, 1385
TauI	GCSGC	1 cut(s)	429
TfiI	GAWTC	2 cut(s)	871, 1199
Tru1I	TTAA	1 cut(s)	1329
Tru9I	TTAA	1 cut(s)	1329
TscAI	CASTG	3 cut(s)	637, 825, 1228
TseFI	GTSAC	4 cut(s)	276, 322, 918, 992
TseI	GCWGC	8 cut(s)	59, 204, 423, 463, 559, 572, 719, 1007
Tsp45I	GTSAC	4 cut(s)	276, 322, 918, 992
TspDTI	ATGAA	8 cut(s)	636, 788, 821, 833, 861, 900, 967, 1481
TspGWI	ACGGA	1 cut(s)	1121
TspRI	CASTG	3 cut(s)	637, 825, 1228
Tth111I	GACNNNGTC	1 cut(s)	1223
Van91I	CCANNNNNTGG	1 cut(s)	1017
VneI	GTGCAC	2 cut(s)	293, 306
VpaK11BI	GGWCC	5 cut(s)	90, 412, 456, 795, 1424
XapI	RAATTY	2 cut(s)	42, 1385
XceI	RCATGY	1 cut(s)	1211
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1191, 1239
XmnI	GAANNNNTTC	1 cut(s)	617
XspI	CTAG	4 cut(s)	242, 932, 1115, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.