Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0012756

Physical Location & Seq

Genomic Coordinates Reverse (-)

22296099 .. 22297233

Gene Structure 1135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 711 bp

ATGGCTCTAGCTACCCAGCCCACCACCAACCTCCTCCAAAACCCCTTCCTCTCCTCCAAACCCCAACCCAGACCCCAGCAGCTCCCCACCGTGGTCCCCTGCCCGGCCCGGCGTCGTTCAGGCTCCGTCCAATGCTCAGTCGCCGTCGCGCCCACCGCGGCCTCCAAGGTCGCCAAGGAGTACAAGGTCAAGTCGGTGAAGGCGAGGCAGATCATTGATAGCAGGGGCAATCCGACCGTCGAGGTTGATCTGGTCACCGACGATCTGTATCGATCGGCCGTGCCCAGTGGCGCTTCTACCGGAATCTACGAGGCGTTGGAGCTCAGGGACGGTGACAAAGCGGTCTACGGCGGGAAAGGTGTGCTCACTGCTGTGAAGAATATCAATGAGATTTTGGGACCCAAGCTCATTGGCGTCGATGTCAGAAATCAAAATGAGGTCGATGCACTTATGCTGGAAATCGATGGAACCCCAAACAAGTCGAAACTGGGGGCCAATGCCATTTTGGGAGTATCTCTGAGCGTGTGTAGAGCGGGAGCAGGAGCAAAGGGAGTGCCATTGTACAAGCACATCCAGGAAGTGTCGGGAATAAAGGAGCTCGTTATGCCAGTCCCTGCTTTCAATGTGATCGATGGAGGCAGCCATGCTGGAAATAATCTGGCAATGCAGGAGTTTATCGATCTTGCCCGTGGGTGCATCCTCGTTCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003824 GO:0004634 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0016043 GO:0016829 GO:0016835 GO:0016836 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

237

Amino Acids

24.89

Weight (kDa)

9.17

Isoelectric Point (pI)

38.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	63 - 191	2.1e-56	Enolase, N-terminal domain
Enolase_C	PF00113	200 - 228	1.1e-08	Enolase, C-terminal TIM barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012756)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G74030
fragaria_vesca	FvH4_5g12990 FvH4_5g12990 FvH4_5g12990
malus_domestica	MD06G1208300.v1.1 MD14G1218700.v1.1
prunus_persica	Prupe.5G212800_v2.0.a1
pyrus_communis	pycom06g18610
rosa_chinensis	RchiOBHm_Chr7g0184181
rosa_laevigata	RLG00000004996
rosa_multiflora	Rmu_sc0012221.1_g000006
rosa_roxburghii	Rroxscaffold_3G00269820
rosa_rugosa	Rorug06G0463700
rosa_samantha	Rh7AG082200 Rh7BG065000 Rh7CG065400 Rh7DG065100
rosa_wichuraiana	Rw7G005330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	341
AccBSI	CCGCTC	1 cut(s)	533
AccI	GTMKAC	1 cut(s)	345
AccII	CGCG	2 cut(s)	149, 158
AciI	CCGC	5 cut(s)	156, 158, 341, 351, 533
AcoI	YGGCCR	1 cut(s)	276
AcyI	GRCGYC	2 cut(s)	112, 414
AfaI	GTAC	2 cut(s)	182, 563
AfiI	CCNNNNNNNGG	2 cut(s)	91, 103
AgsI	TTSAA	1 cut(s)	622
AjnI	CCWGG	1 cut(s)	573
AleI	CACNNNNGTG	1 cut(s)	371
AluBI	AGCT	5 cut(s)	11, 82, 322, 406, 598
AluI	AGCT	5 cut(s)	11, 82, 322, 406, 598
Alw21I	GWGCWC	3 cut(s)	324, 366, 600
AlwNI	CAGNNNCTG	1 cut(s)	614
AoxI	GGCC	4 cut(s)	105, 159, 276, 492
ApeKI	GCWGC	2 cut(s)	79, 639
AspLEI	GCGC	2 cut(s)	151, 293
AspS9I	GGNCC	4 cut(s)	94, 106, 398, 492
AsuC2I	CCSGG	2 cut(s)	104, 109
AsuHPI	GGTGA	3 cut(s)	208, 247, 344
AvaII	GGWCC	2 cut(s)	94, 398
BaeGI	GKGCMC	1 cut(s)	285
BanII	GRGCYC	2 cut(s)	324, 600
Bbv12I	GWGCWC	3 cut(s)	324, 366, 600
BbvI	GCAGC	2 cut(s)	91, 651
BccI	CCATC	2 cut(s)	458, 626
BceAI	ACGGC	3 cut(s)	128, 263, 364
BciT130I	CCWGG	1 cut(s)	575
BcnI	CCSGG	2 cut(s)	104, 109
BfaI	CTAG	1 cut(s)	8
BfoI	RGCGCY	1 cut(s)	294
BisI	GCNGC	3 cut(s)	80, 159, 640
BlsI	GCNGC	3 cut(s)	81, 160, 641
Bme1390I	CCNGG	3 cut(s)	104, 109, 575
Bme18I	GGWCC	2 cut(s)	94, 398
BmgT120I	GGNCC	4 cut(s)	94, 106, 398, 492
BmiI	GGNNCC	6 cut(s)	96, 124, 399, 400, 469, 493
BmrFI	CCNGG	3 cut(s)	104, 109, 575
BmrI	ACTGGG	2 cut(s)	279, 497
BmsI	GCATC	2 cut(s)	433, 705
BmuI	ACTGGG	2 cut(s)	279, 497
Bpu10I	CCTNAGC	1 cut(s)	323
BpuMI	CCSGG	2 cut(s)	104, 109
Bsa29I	ATCGAT	4 cut(s)	271, 462, 630, 678
BsaBI	GATNNNNATC	1 cut(s)	267
BsaHI	GRCGYC	2 cut(s)	112, 414
BsaJI	CCNNGG	5 cut(s)	90, 156, 165, 174, 688
BsaWI	WCCGGW	1 cut(s)	299
Bsc4I	CCNNNNNNNGG	2 cut(s)	91, 103
Bse1I	ACTGG	3 cut(s)	285, 492, 608
Bse3DI	GCAATG	1 cut(s)	669
Bse8I	GATNNNNATC	1 cut(s)	267
BseBI	CCWGG	1 cut(s)	575
BseCI	ATCGAT	4 cut(s)	271, 462, 630, 678
BseDI	CCNNGG	5 cut(s)	90, 156, 165, 174, 688
BseGI	GGATG	2 cut(s)	570, 696
BseJI	GATNNNNATC	1 cut(s)	267
BseLI	CCNNNNNNNGG	2 cut(s)	91, 103
BseMI	GCAATG	1 cut(s)	669
BseMII	CTCAG	3 cut(s)	150, 337, 509
BseNI	ACTGG	3 cut(s)	285, 492, 608
BseRI	GAGGAG	2 cut(s)	23, 43
BseSI	GKGCMC	1 cut(s)	285
BseX3I	CGGCCG	1 cut(s)	276
BseXI	GCAGC	2 cut(s)	91, 651
BseYI	CCCAGC	2 cut(s)	15, 75
Bsh1236I	CGCG	2 cut(s)	149, 158
Bsh1285I	CGRYCG	3 cut(s)	237, 275, 279
BshFI	GGCC	4 cut(s)	107, 161, 278, 494
BshVI	ATCGAT	4 cut(s)	271, 462, 630, 678
BsiEI	CGRYCG	3 cut(s)	237, 275, 279
BsiHKAI	GWGCWC	3 cut(s)	324, 366, 600
BsiSI	CCGG	3 cut(s)	104, 109, 300
BslFI	GGGAC	4 cut(s)	80, 341, 411, 596
BslI	CCNNNNNNNGG	2 cut(s)	91, 103
BsmFI	GGGAC	4 cut(s)	80, 341, 411, 596
BsnI	GGCC	4 cut(s)	107, 161, 278, 494
Bsp1286I	GDGCHC	4 cut(s)	285, 324, 366, 600
Bsp1407I	TGTACA	1 cut(s)	561
Bsp143I	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
BspACI	CCGC	5 cut(s)	156, 158, 341, 351, 533
BspANI	GGCC	4 cut(s)	107, 161, 278, 494
BspCNI	CTCAG	3 cut(s)	149, 336, 510
BspDI	ATCGAT	4 cut(s)	271, 462, 630, 678
BspFNI	CGCG	2 cut(s)	149, 158
BspLI	GGNNCC	6 cut(s)	96, 124, 399, 400, 469, 493
BsrBI	CCGCTC	1 cut(s)	533
BsrDI	GCAATG	1 cut(s)	669
BsrGI	TGTACA	1 cut(s)	561
BsrI	ACTGG	3 cut(s)	285, 492, 608
BssECI	CCNNGG	5 cut(s)	90, 156, 165, 174, 688
BssMI	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
BssNI	GRCGYC	2 cut(s)	112, 414
BssT1I	CCWWGG	2 cut(s)	165, 174
Bst2UI	CCWGG	1 cut(s)	575
Bst4CI	ACNGT	3 cut(s)	91, 238, 332
BstACI	GRCGYC	2 cut(s)	112, 414
BstAUI	TGTACA	1 cut(s)	561
BstDEI	CTNAG	3 cut(s)	136, 323, 518
BstDSI	CCRYGG	3 cut(s)	90, 156, 688
BstEII	GGTNACC	1 cut(s)	253
BstF5I	GGATG	2 cut(s)	570, 696
BstFNI	CGCG	2 cut(s)	149, 158
BstH2I	RGCGCY	1 cut(s)	294
BstHHI	GCGC	2 cut(s)	151, 293
BstKTI	GATC	6 cut(s)	213, 250, 265, 275, 630, 682
BstMBI	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
BstMCI	CGRYCG	3 cut(s)	237, 275, 279
BstMWI	GCNNNNNNNGC	2 cut(s)	155, 604
BstNI	CCWGG	1 cut(s)	575
BstPI	GGTNACC	1 cut(s)	253
BstSCI	CCNGG	3 cut(s)	102, 107, 573
BstSLI	GKGCMC	1 cut(s)	285
BstUI	CGCG	2 cut(s)	149, 158
BstV1I	GCAGC	2 cut(s)	91, 651
BstZI	CGGCCG	1 cut(s)	276
Bsu15I	ATCGAT	4 cut(s)	271, 462, 630, 678
BsuRI	GGCC	4 cut(s)	107, 161, 278, 494
BsuTUI	ATCGAT	4 cut(s)	271, 462, 630, 678
BtgI	CCRYGG	3 cut(s)	90, 156, 688
BtsCI	GGATG	2 cut(s)	570, 696
BtsI	GCAGTG	1 cut(s)	366
BtsIMutI	CAGTG	2 cut(s)	292, 366
CaiI	CAGNNNCTG	1 cut(s)	614
CfoI	GCGC	2 cut(s)	151, 293
Cfr13I	GGNCC	4 cut(s)	94, 106, 398, 492
Cfr42I	CCGCGG	1 cut(s)	159
ClaI	ATCGAT	4 cut(s)	271, 462, 630, 678
CseI	GACGC	2 cut(s)	101, 403
Csp6I	GTAC	2 cut(s)	181, 562
CviAII	CATG	1 cut(s)	644
CviQI	GTAC	2 cut(s)	181, 562
DdeI	CTNAG	3 cut(s)	136, 323, 518
DpnI	GATC	6 cut(s)	212, 249, 264, 274, 629, 681
DpnII	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
DrdI	GACNNNNNNGTC	1 cut(s)	341
DseDI	GACNNNNNNGTC	1 cut(s)	341
EaeI	YGGCCR	1 cut(s)	276
EagI	CGGCCG	1 cut(s)	276
Ecl136II	GAGCTC	2 cut(s)	322, 598
EclXI	CGGCCG	1 cut(s)	276
Eco130I	CCWWGG	2 cut(s)	165, 174
Eco24I	GRGCYC	2 cut(s)	324, 600
Eco47I	GGWCC	2 cut(s)	94, 398
Eco52I	CGGCCG	1 cut(s)	276
Eco53kI	GAGCTC	2 cut(s)	322, 598
Eco91I	GGTNACC	1 cut(s)	253
EcoICRI	GAGCTC	2 cut(s)	322, 598
EcoO109I	RGGNCCY	1 cut(s)	398
EcoO65I	GGTNACC	1 cut(s)	253
EcoRII	CCWGG	1 cut(s)	573
EcoT14I	CCWWGG	2 cut(s)	165, 174
EcoT38I	GRGCYC	2 cut(s)	324, 600
ErhI	CCWWGG	2 cut(s)	165, 174
FaeI	CATG	1 cut(s)	647
FaiI	YATR	3 cut(s)	452, 605, 645
FaqI	GGGAC	4 cut(s)	80, 341, 411, 596
FatI	CATG	1 cut(s)	643
FauI	CCCGC	2 cut(s)	344, 526
FblI	GTMKAC	1 cut(s)	345
Fnu4HI	GCNGC	3 cut(s)	80, 159, 640
FokI	GGATG	2 cut(s)	557, 683
FriOI	GRGCYC	2 cut(s)	324, 600
Fsp4HI	GCNGC	3 cut(s)	80, 159, 640
FspBI	CTAG	1 cut(s)	8
GlaI	GCGC	2 cut(s)	150, 292
GluI	GCNGC	3 cut(s)	80, 159, 640
GsaI	CCCAGC	2 cut(s)	19, 79
HaeII	RGCGCY	1 cut(s)	294
HaeIII	GGCC	4 cut(s)	107, 161, 278, 494
HapII	CCGG	3 cut(s)	104, 109, 300
HgaI	GACGC	2 cut(s)	101, 403
HhaI	GCGC	2 cut(s)	151, 293
Hin1I	GRCGYC	2 cut(s)	112, 414
Hin1II	CATG	1 cut(s)	647
Hin6I	GCGC	2 cut(s)	149, 291
HinP1I	GCGC	2 cut(s)	149, 291
HinfI	GANTC	1 cut(s)	303
HpaII	CCGG	3 cut(s)	104, 109, 300
HphI	GGTGA	3 cut(s)	208, 247, 344
Hpy166II	GTNNAC	1 cut(s)	346
Hpy188I	TCNGA	3 cut(s)	234, 425, 519
Hpy188III	TCNNGA	1 cut(s)	585
Hpy8I	GTNNAC	1 cut(s)	346
Hpy99I	CGWCG	5 cut(s)	117, 149, 242, 263, 419
HpyAV	CCTTC	2 cut(s)	55, 193
HpyCH4III	ACNGT	3 cut(s)	91, 238, 332
HpyCH4V	TGCA	3 cut(s)	446, 667, 696
HpyF10VI	GCNNNNNNNGC	2 cut(s)	155, 604
HpyF3I	CTNAG	3 cut(s)	136, 323, 518
Hsp92I	GRCGYC	2 cut(s)	112, 414
Hsp92II	CATG	1 cut(s)	647
HspAI	GCGC	2 cut(s)	149, 291
KflI	GGGWCCC	1 cut(s)	398
KspI	CCGCGG	1 cut(s)	159
Kzo9I	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
LmnI	GCTCC	6 cut(s)	87, 128, 319, 536, 542, 595
Lsp1109I	GCAGC	2 cut(s)	91, 651
LweI	GCATC	2 cut(s)	433, 705
MaeI	CTAG	1 cut(s)	8
MaeIII	GTNAC	2 cut(s)	253, 332
MalI	GATC	6 cut(s)	212, 249, 264, 274, 629, 681
MbiI	CCGCTC	1 cut(s)	533
MboI	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
MboII	GAAGA	1 cut(s)	388
MhlI	GDGCHC	4 cut(s)	285, 324, 366, 600
MmeI	TCCRAC	2 cut(s)	257, 297
MslI	CAYNNNNRTG	1 cut(s)	371
MspA1I	CMGCKG	1 cut(s)	158
MspI	CCGG	3 cut(s)	104, 109, 300
MspR9I	CCNGG	3 cut(s)	104, 109, 575
MvaI	CCWGG	1 cut(s)	575
MvnI	CGCG	2 cut(s)	149, 158
MwoI	GCNNNNNNNGC	2 cut(s)	155, 604
NciI	CCSGG	2 cut(s)	104, 109
NdeII	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
NlaIII	CATG	1 cut(s)	647
NlaIV	GGNNCC	6 cut(s)	96, 124, 399, 400, 469, 493
NmuCI	GTSAC	2 cut(s)	253, 332
OliI	CACNNNNGTG	1 cut(s)	371
PcsI	WCGNNNNNNNCGW	1 cut(s)	200
PfeI	GAWTC	1 cut(s)	303
PfoI	TCCNGGA	1 cut(s)	573
PkrI	GCNGC	3 cut(s)	81, 160, 641
Ple19I	CGATCG	1 cut(s)	275
PpuMI	RGGWCCY	1 cut(s)	398
Psp124BI	GAGCTC	2 cut(s)	324, 600
Psp5II	RGGWCCY	1 cut(s)	398
Psp6I	CCWGG	1 cut(s)	573
PspEI	GGTNACC	1 cut(s)	253
PspFI	CCCAGC	2 cut(s)	15, 75
PspGI	CCWGG	1 cut(s)	573
PspN4I	GGNNCC	6 cut(s)	96, 124, 399, 400, 469, 493
PspPI	GGNCC	4 cut(s)	94, 106, 398, 492
PspPPI	RGGWCCY	1 cut(s)	398
PstNI	CAGNNNCTG	1 cut(s)	614
PvuI	CGATCG	1 cut(s)	275
RsaI	GTAC	2 cut(s)	182, 563
RsaNI	GTAC	2 cut(s)	181, 562
RseI	CAYNNNNRTG	1 cut(s)	371
SacI	GAGCTC	2 cut(s)	324, 600
SacII	CCGCGG	1 cut(s)	159
SatI	GCNGC	3 cut(s)	80, 159, 640
Sau3AI	GATC	6 cut(s)	210, 247, 262, 272, 627, 679
Sau96I	GGNCC	4 cut(s)	94, 106, 398, 492
ScrFI	CCNGG	3 cut(s)	104, 109, 575
SduI	GDGCHC	4 cut(s)	285, 324, 366, 600
SfaNI	GCATC	2 cut(s)	433, 705
Sfr303I	CCGCGG	1 cut(s)	159
SgrBI	CCGCGG	1 cut(s)	159
SinI	GGWCC	2 cut(s)	94, 398
SmiMI	CAYNNNNRTG	1 cut(s)	371
SsiI	CCGC	5 cut(s)	156, 158, 341, 351, 533
SspMI	CTAG	1 cut(s)	8
SstI	GAGCTC	2 cut(s)	324, 600
StyD4I	CCNGG	3 cut(s)	102, 107, 573
StyI	CCWWGG	2 cut(s)	165, 174
TaaI	ACNGT	3 cut(s)	91, 238, 332
TaqI	TCGA	8 cut(s)	240, 271, 417, 441, 462, 482, 630, 678
TatI	WGTACW	2 cut(s)	180, 561
TauI	GCSGC	1 cut(s)	161
TfiI	GAWTC	1 cut(s)	303
TscAI	CASTG	2 cut(s)	292, 373
TseFI	GTSAC	2 cut(s)	253, 332
TseI	GCWGC	2 cut(s)	79, 639
Tsp45I	GTSAC	2 cut(s)	253, 332
TspGWI	ACGGA	1 cut(s)	115
TspRI	CASTG	2 cut(s)	292, 373
VpaK11BI	GGWCC	2 cut(s)	94, 398
XcmI	CCANNNNNNNNNTGG	1 cut(s)	502
XmiI	GTMKAC	1 cut(s)	345
XspI	CTAG	1 cut(s)	8

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.