pycom07g04970
ERF Family

Performs the first committed step in the biosynthesis of isoprene-containing compounds such as sterols and terpenoids

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010940
Physical Location & Seq
Forward (+)
4250415 .. 4255521
5107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGGCGGATGAGTCGCAGAAATGGGTGCTGATGGTGACGGCTCAGACGCCCACCAACATCGCCGTGATCAAGTACTGGGGGAAGAGGGACGAGAAGCTCATCCTGCCGGTCAATGACAGCATCAGCGTGACGCTTGATCCGGCCCACCTCTGCACCACCACCAGTGTCTCCGTCAGTCCTACCTTTGACCAAGATCGGATGTGGCTCAATGGCGAGGAGATATCCATTTCTGGAGGCAGATTCCAAAGTTGTTTAAGAGAAATTCGCAGTCGAGCTACCGATGTTGATGATAAGGAAAAGGGAATCAAGATTACTAAGAAGGATTGGGAAAAATTGCATGTACATATCTCTTCTTATAACAATTTTCCTACTGCTGCTGGATTGGCTTCCTCAGCTGCTGGTTTTGCTTGTCTAGTTTTTGCCCTCGGAAAGTTAATGAATGTGAAAGAAGATCACAGCCAGCTTTCTGCTATTGCAAGGCAAGGTTCTGGTAGTGCTTGCCGCAGCTTATATGGGGGATTTGTCAAGTGGATCGATATGGGGAAAGATGATAAGGGAACCGACAGTCTTGCAGTCCAACTTGCAGATGAGAAGCACTGGGATGAACTTGTTATTGTTATTGCTGTGGTAAGTTCACGGCAGAAGGAAACAAGTAGCACTACAGGAATGCGTGATACTGTTGAAACAAGTTTGCTCTTACAACATAGAGCAAAGGAAGTAGTGCCAAAACGCATATTGAAAATGGAAGAAGCCATAAAAAATCGTGATTTTGCTTCTTTTGCACAACTGACTTGTACTGACAGTAACCAGTTTCACGCTGTCTGCCTGGACACATGTCCACCCATCTTCTACATGAATGATACATCCCACAGGCAAGCCCCGATAATAAGCCTTGTTGAAAAGTGGAACCGCGCAGAAGGAACACCTCAGGTGGCTTATACATTTGATGCGGGGCCGAATGCAGTTCTAATTGCACGTAACAGAAAAACAGCTGCCCTTTTAATTCAGAAGTTGCTATTCTGCTTTCCACCAAATTCTGATGCAGATTTGAACAGTTTCGTACTTGGTGATAAGTCCATTCTAAAAGATGCTGGGGTTGAGAGCCCGGAGGATATTGAAACTTTACCCCCACCTCCAGAAATCAAGGATCCGTCCCAGAAATTCAAAGGAGATGTCAGTTACTTTATTTGCACAAGACCTGGGAGAGGTCCAGTTGTGCTGTCAGATGAAAGTCATCTTCTCAACCCTGAAACTGGATTGCCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004163 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006066 GO:0006084 GO:0006139 GO:0006163 GO:0006464 GO:0006486 GO:0006487 GO:0006488 GO:0006489 GO:0006490 GO:0006629 GO:0006637 GO:0006644 GO:0006694 GO:0006695 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008202 GO:0008203 GO:0008284 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009889 GO:0009987 GO:0016125 GO:0016126 GO:0016829 GO:0016830 GO:0016831 GO:0019216 GO:0019218 GO:0019222 GO:0019287 GO:0019538 GO:0019637 GO:0019693 GO:0030544 GO:0031072 GO:0033865 GO:0033875 GO:0034032 GO:0034641 GO:0034645 GO:0035383 GO:0036211 GO:0042127 GO:0042579 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0045540 GO:0046165 GO:0046465 GO:0046483 GO:0046490 GO:0046890 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050810 GO:0051186 GO:0055086 GO:0062012 GO:0065007 GO:0070085 GO:0071704 GO:0072521 GO:0080090 GO:0090181 GO:0090407 GO:0106118 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902652 GO:1902653 GO:1902930
KEGG Pathways
Metabolic & Signaling
ko00900 ko01100 ko01110 ko01130 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
GHMP_kinases_N

Protein Analysis

423

Amino Acids

46.58

Weight (kDa)

6.04

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MVD-like_N PF22700 17 - 191 8.6e-64 Diphosphomevalonate decarboxylase-like N-terminal domain
GHMP_kinases_N PF00288 105 - 166 2.6e-06 GHMP kinases N terminal domain
MDD_C PF18376 205 - 406 2.5e-63 Mevalonate 5-diphosphate decarboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38700 AT3G54250
fragaria_vesca FvH4_7g05610 FvH4_7g05610
malus_domestica MD02G1256300.v1.1 MD07G1064100.v1.1
prunus_persica Prupe.2G078900_v2.0.a1
pyrus_communis pycom02g21690 pycom07g04970
rosa_chinensis RchiOBHm_Chr1g0332421
rosa_laevigata RLG00000029671
rosa_multiflora Rmu_sc0016943.1_g000003 Rmu_ssc0000161.1_g000007
rosa_roxburghii Rroxscaffold_4G00318190
rosa_rugosa Rorug01G0100700
rosa_samantha Rh1AG124800 Rh1BG095200 Rh1CG118900 Rh1DG129700
rosa_wichuraiana Rw1G010140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AccII CGCG 1 cut(s) 912
AciI CCGC 4 cut(s) 5, 502, 910, 950
AclWI GGATC 4 cut(s) 131, 539, 1142, 1155
AcsI RAATTY 3 cut(s) 261, 1033, 1160
AcyI GRCGYC 1 cut(s) 47
AfaI GTAC 4 cut(s) 74, 342, 796, 1062
AfiI CCNNNNNNNGG 2 cut(s) 1205, 1253
AflIII ACRYGT 1 cut(s) 833
AgsI TTSAA 6 cut(s) 683, 739, 899, 1051, 1118, 1165
AjnI CCWGG 2 cut(s) 825, 1198
AluBI AGCT 6 cut(s) 97, 275, 395, 463, 507, 992
AluI AGCT 6 cut(s) 97, 275, 395, 463, 507, 992
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 4 cut(s) 131, 539, 1142, 1155
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 2 cut(s) 141, 953
ApeKI GCWGC 4 cut(s) 374, 395, 504, 992
ApoI RAATTY 3 cut(s) 261, 1033, 1160
ArsI GACNNNNNNTTYG 2 cut(s) 1158, 1190
Asp700I GAANNNNTTC 1 cut(s) 1055
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 3 cut(s) 142, 953, 1208
AsuC2I CCSGG 1 cut(s) 1106
AsuHPI GGTGA 2 cut(s) 46, 1079
AvaII GGWCC 1 cut(s) 1208
AxyI CCTNAGG 1 cut(s) 927
BamHI GGATCC 1 cut(s) 1147
BanII GRGCYC 1 cut(s) 1106
BbvCI CCTCAGC 1 cut(s) 391
BbvI GCAGC 4 cut(s) 361, 382, 516, 979
BccI CCATC 2 cut(s) 25, 851
BceAI ACGGC 3 cut(s) 47, 54, 653
BcgI CGANNNNNNTGC 2 cut(s) 551, 585
BciT130I CCWGG 2 cut(s) 827, 1200
BclI TGATCA 1 cut(s) 66
BcnI CCSGG 1 cut(s) 1106
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 660
BisI GCNGC 5 cut(s) 375, 396, 502, 505, 993
BlsI GCNGC 5 cut(s) 376, 397, 503, 506, 994
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 3 cut(s) 827, 1106, 1200
Bme18I GGWCC 1 cut(s) 1208
BmgT120I GGNCC 3 cut(s) 142, 953, 1208
BmiI GGNNCC 4 cut(s) 559, 908, 954, 1149
BmrFI CCNGG 3 cut(s) 827, 1106, 1200
BmrI ACTGGG 2 cut(s) 85, 607
BmsI GCATC 4 cut(s) 129, 937, 1030, 1078
BmuI ACTGGG 2 cut(s) 85, 607
BoxI GACNNNNGTC 1 cut(s) 834
BpmI CTGGAG 2 cut(s) 252, 1119
Bpu10I CCTNAGC 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 1106
Bsa29I ATCGAT 1 cut(s) 534
BsaAI YACGTR 1 cut(s) 977
BsaHI GRCGYC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 424, 1199
Bsc4I CCNNNNNNNGG 2 cut(s) 1205, 1253
Bse118I RCCGGY 1 cut(s) 106
Bse1I ACTGG 6 cut(s) 80, 162, 602, 808, 1211, 1258
Bse21I CCTNAGG 1 cut(s) 927
BseBI CCWGG 2 cut(s) 827, 1200
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 424, 1199
BseGI GGATG 5 cut(s) 13, 99, 204, 607, 863
BseLI CCNNNNNNNGG 2 cut(s) 1205, 1253
BseMII CTCAG 3 cut(s) 56, 405, 941
BseNI ACTGG 6 cut(s) 80, 162, 602, 808, 1211, 1258
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 4 cut(s) 361, 382, 516, 979
BseYI CCCAGC 1 cut(s) 1091
BsgI GTGCAG 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 912
BshFI GGCC 2 cut(s) 143, 955
BshVI ATCGAT 1 cut(s) 534
BsiSI CCGG 3 cut(s) 107, 140, 1106
BslFI GGGAC 2 cut(s) 101, 1138
BslI CCNNNNNNNGG 2 cut(s) 1205, 1253
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 101, 1138
BsmI GAATGC 2 cut(s) 672, 964
BsnI GGCC 2 cut(s) 143, 955
Bsp1286I GDGCHC 1 cut(s) 1106
Bsp1407I TGTACA 1 cut(s) 340
Bsp143I GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
BspACI CCGC 4 cut(s) 5, 502, 910, 950
BspANI GGCC 2 cut(s) 143, 955
BspCNI CTCAG 3 cut(s) 55, 404, 940
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 1 cut(s) 912
BspLI GGNNCC 4 cut(s) 559, 908, 954, 1149
BspPI GGATC 4 cut(s) 131, 539, 1142, 1155
BsrFI RCCGGY 1 cut(s) 106
BsrGI TGTACA 1 cut(s) 340
BsrI ACTGG 6 cut(s) 80, 162, 602, 808, 1211, 1258
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 2 cut(s) 424, 1199
BssMI GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
BssNI GRCGYC 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 827, 1200
Bst4CI ACNGT 4 cut(s) 566, 679, 803, 1055
Bst6I CTCTTC 2 cut(s) 77, 355
BstACI GRCGYC 1 cut(s) 47
BstAUI TGTACA 1 cut(s) 340
BstBAI YACGTR 1 cut(s) 977
BstC8I GCNNGC 3 cut(s) 461, 499, 876
BstDEI CTNAG 4 cut(s) 42, 315, 391, 927
BstENI CCTNNNNNAGG 1 cut(s) 1203
BstF5I GGATG 5 cut(s) 13, 99, 204, 607, 863
BstFNI CGCG 1 cut(s) 912
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 6 cut(s) 69, 139, 196, 454, 534, 1150
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
BstMWI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 779
BstNI CCWGG 2 cut(s) 827, 1200
BstNSI RCATGY 2 cut(s) 341, 837
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 3 cut(s) 825, 1104, 1198
BstSFI CTRYAG 1 cut(s) 660
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 4 cut(s) 361, 382, 516, 979
BstX2I RGATCY 1 cut(s) 1147
BstYI RGATCY 1 cut(s) 1147
Bsu15I ATCGAT 1 cut(s) 534
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 2 cut(s) 143, 955
BsuTUI ATCGAT 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 5 cut(s) 13, 99, 204, 607, 863
BtsIMutI CAGTG 2 cut(s) 169, 595
Cac8I GCNNGC 3 cut(s) 461, 499, 876
CaiI CAGNNNCTG 1 cut(s) 398
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 3 cut(s) 142, 953, 1208
ClaI ATCGAT 1 cut(s) 534
CseI GACGC 2 cut(s) 55, 139
Csp6I GTAC 4 cut(s) 73, 341, 795, 1061
CviAII CATG 3 cut(s) 338, 834, 853
CviQI GTAC 4 cut(s) 73, 341, 795, 1061
DdeI CTNAG 4 cut(s) 42, 315, 391, 927
DpnI GATC 6 cut(s) 68, 138, 195, 453, 533, 1149
DpnII GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
Eam1104I CTCTTC 2 cut(s) 77, 355
EarI CTCTTC 2 cut(s) 77, 355
EciI GGCGGA 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 1106
Eco32I GATATC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 1208
Eco81I CCTNAGG 1 cut(s) 927
EcoNI CCTNNNNNAGG 1 cut(s) 1203
EcoRII CCWGG 2 cut(s) 825, 1198
EcoRV GATATC 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 1106
FaeI CATG 3 cut(s) 341, 837, 856
FaqI GGGAC 2 cut(s) 101, 1138
FatI CATG 3 cut(s) 337, 833, 852
FauI CCCGC 1 cut(s) 943
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 5 cut(s) 375, 396, 502, 505, 993
FokI GGATG 5 cut(s) 20, 86, 211, 614, 850
FriOI GRGCYC 1 cut(s) 1106
Fsp4HI GCNGC 5 cut(s) 375, 396, 502, 505, 993
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 913
GluI GCNGC 5 cut(s) 375, 396, 502, 505, 993
GsaI CCCAGC 1 cut(s) 1095
GsuI CTGGAG 2 cut(s) 252, 1119
HaeIII GGCC 2 cut(s) 143, 955
HapII CCGG 3 cut(s) 107, 140, 1106
HgaI GACGC 2 cut(s) 55, 139
HhaI GCGC 1 cut(s) 914
Hin1I GRCGYC 1 cut(s) 47
Hin1II CATG 3 cut(s) 341, 837, 856
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HinfI GANTC 3 cut(s) 11, 240, 303
HpaII CCGG 3 cut(s) 107, 140, 1106
HphI GGTGA 2 cut(s) 46, 1079
Hpy166II GTNNAC 2 cut(s) 635, 839
Hpy188I TCNGA 6 cut(s) 45, 198, 428, 1008, 1039, 1225
Hpy188III TCNNGA 4 cut(s) 231, 307, 764, 1136
Hpy8I GTNNAC 2 cut(s) 635, 839
HpyAV CCTTC 3 cut(s) 313, 637, 911
HpyCH4III ACNGT 4 cut(s) 566, 679, 803, 1055
HpyCH4IV ACGT 1 cut(s) 976
HpyF10VI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 779
HpyF3I CTNAG 4 cut(s) 42, 315, 391, 927
HpySE526I ACGT 1 cut(s) 976
Hsp92I GRCGYC 1 cut(s) 47
Hsp92II CATG 3 cut(s) 341, 837, 856
HspAI GCGC 1 cut(s) 912
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
Lsp1109I GCAGC 4 cut(s) 361, 382, 516, 979
LweI GCATC 4 cut(s) 129, 937, 1030, 1078
MaeI CTAG 1 cut(s) 413
MaeII ACGT 1 cut(s) 976
MaeIII GTNAC 5 cut(s) 34, 127, 803, 977, 1178
MalI GATC 6 cut(s) 68, 138, 195, 453, 533, 1149
MboI GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
MboII GAAGA 6 cut(s) 94, 342, 461, 758, 838, 1229
MflI RGATCY 1 cut(s) 1147
MhlI GDGCHC 1 cut(s) 1106
MluCI AATT 7 cut(s) 261, 332, 361, 969, 1002, 1033, 1160
MlyI GAGTC 1 cut(s) 20
MmeI TCCRAC 1 cut(s) 601
MroXI GAANNNNTTC 1 cut(s) 1055
MseI TTAA 3 cut(s) 254, 434, 1001
MslI CAYNNNNRTG 3 cut(s) 62, 125, 600
MspA1I CMGCKG 2 cut(s) 395, 992
MspI CCGG 3 cut(s) 107, 140, 1106
MspR9I CCNGG 3 cut(s) 827, 1106, 1200
Mva1269I GAATGC 2 cut(s) 672, 964
MvaI CCWGG 2 cut(s) 827, 1200
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 5 cut(s) 103, 383, 392, 404, 779
NciI CCSGG 1 cut(s) 1106
NdeII GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
NlaIII CATG 3 cut(s) 341, 837, 856
NlaIV GGNNCC 4 cut(s) 559, 908, 954, 1149
NmuCI GTSAC 2 cut(s) 34, 127
NspI RCATGY 2 cut(s) 341, 837
PciI ACATGT 1 cut(s) 833
PctI GAATGC 2 cut(s) 672, 964
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 2 cut(s) 240, 303
PkrI GCNGC 5 cut(s) 376, 397, 503, 506, 994
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
Ppu21I YACGTR 1 cut(s) 977
PscI ACATGT 1 cut(s) 833
PshAI GACNNNNGTC 1 cut(s) 834
PsiI TTATAA 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 825, 1198
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 2 cut(s) 825, 1198
PspN4I GGNNCC 4 cut(s) 559, 908, 954, 1149
PspPI GGNCC 3 cut(s) 142, 953, 1208
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 1 cut(s) 1147
PvuII CAGCTG 2 cut(s) 395, 992
RsaI GTAC 4 cut(s) 74, 342, 796, 1062
RsaNI GTAC 4 cut(s) 73, 341, 795, 1061
RseI CAYNNNNRTG 3 cut(s) 62, 125, 600
SaqAI TTAA 3 cut(s) 254, 434, 1001
SatI GCNGC 5 cut(s) 375, 396, 502, 505, 993
Sau3AI GATC 6 cut(s) 66, 136, 193, 451, 531, 1147
Sau96I GGNCC 3 cut(s) 142, 953, 1208
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 1 cut(s) 20
ScrFI CCNGG 3 cut(s) 827, 1106, 1200
SduI GDGCHC 1 cut(s) 1106
SfaNI GCATC 4 cut(s) 129, 937, 1030, 1078
SfcI CTRYAG 1 cut(s) 660
SinI GGWCC 1 cut(s) 1208
SmiMI CAYNNNNRTG 3 cut(s) 62, 125, 600
Sse9I AATT 7 cut(s) 261, 332, 361, 969, 1002, 1033, 1160
SsiI CCGC 4 cut(s) 5, 502, 910, 950
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 825, 1104, 1198
TaaI ACNGT 4 cut(s) 566, 679, 803, 1055
TaiI ACGT 1 cut(s) 979
TaqI TCGA 2 cut(s) 271, 534
TasI AATT 7 cut(s) 261, 332, 361, 969, 1002, 1033, 1160
TatI WGTACW 3 cut(s) 72, 340, 794
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 2 cut(s) 240, 303
Tru1I TTAA 3 cut(s) 254, 434, 1001
Tru9I TTAA 3 cut(s) 254, 434, 1001
TscAI CASTG 2 cut(s) 169, 602
TseFI GTSAC 2 cut(s) 34, 127
TseI GCWGC 4 cut(s) 374, 395, 504, 992
Tsp45I GTSAC 2 cut(s) 34, 127
TspDTI ATGAA 4 cut(s) 452, 618, 869, 1242
TspGWI ACGGA 2 cut(s) 160, 1140
TspRI CASTG 2 cut(s) 169, 602
VpaK11BI GGWCC 1 cut(s) 1208
XagI CCTNNNNNAGG 1 cut(s) 1203
XapI RAATTY 3 cut(s) 261, 1033, 1160
XceI RCATGY 2 cut(s) 341, 837
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 1 cut(s) 413
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.