Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0007863

Physical Location & Seq

Genomic Coordinates Reverse (-)

22368476 .. 22381754

Gene Structure 13279 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5484 bp

ATGTCGCCGTTGGCCGTTCGCCGCCTCACGGTGCTCGGCGAGTTCAAGCCGTTCGGATTAATCGCCGAAGCTCTCGACGGTAAGCCCGCCGATAACGTCACCGACAAGTATGATTACTTCCTCTTTGACCCCGAAACCGTCCGAGAACTAGACGAAACGGATGACAATGACTCTTCGGCTTCCGCGCTGAGCGATCGGTGCGATCACGAGCTGTTCATTCGACGAAATCGGATAATATGGTCAACCGGTCCGCGAGTGTTTAAGAGATTCACATTGCCGTCTCCAGTAATCACGGCTTGCTGGTGTCGCTTGGGGGATATGACCGAAGCGCTTCTATGCGTTTTACAAGTTGCTTGTTTAACAATCTACAACACATCAGGTGAAGTAGTCTCTGTCCCTCTTTCTCGAACCGTCACATCGGTATGGCCTCTTCCATTTGGTTTGTTACTTCAGCAGGCAGCTGAAGTGAACTCAACTATACATGCTCCTTCTTCATCTTCGACTACTTTGTTTGGCGTACGAGATCTCCCTCGTCCTAGAAGAGAAAGTGGACATAGTCCGCAGCATAATGTCAATTTTGTGACTTCACTTGACCATATCGCCAAGGGAGAAACAAGCCCGGTGTCCTCACATCTGATTTTGAAAGATCCATTAGAAGAACCACACTTGGCTCATATTGAAGAAAGAGGAAAACTGAACTTAATGAAGGAATTTGATGAAACTACTATTTGGACAAGTGATCGAATTCCTCTTATGGCATCATATAATACAGGAAAGATGCAACATTCTGTGTGGGTTGCGGAAACCAGTAATTCTAACCATGAAATGGCAAGTGCTAGTTTAGTAGATGCAGTTCCTGCTGGTGTTCTAGCAAAACAATTCTCATTTCGTAGAATATGGCAAGGCAAGGGTGCCCATACAGCAGCTTGTAAGGTCTTTCTAGCGACTGACGATGATGCAGCTCCTATTATTTGTTTTCTTCATCAAGAACAAAAAAAATTGATGTGTGTAAGACTTCAAAGCGTTGAGATAAATAATGAAATTATATTTGACATTAAACCCGACATGAGCTGGAGCATACCTGCAGTTGCAGCAGCACCTGTGATTGTTACCCGCCCTAGGGCGAAAGTGGGACTGCTGCCATATTCAGACATGCTTGTTCTGGCTCCTGAAAATGTCCTCCTTCTCTATTCGGGGAAACATTGCCTGTGTAGGTATATGTTGCCTTCTTGTGTTAGTGAAGGCCAATTTTCACATAATTTAGAGTTTCCAGAAACATCTGTTACTCAGGGCCTGAAGGTTATAGGATTGGCCGATGCTGTTGAGGGGCGTATCAATGTTACTGTAAATAATGGGAAGATGTTTCGGTGTGTATTACGTAGAAGCCCTGCATCTACCCTGGCAAATGATTGCATCACAGCCATGGCTGAGGGACTAAGTACCAGTTTCTATAGCCATTTTCTTAGTCTGCTTTGGAAAGATGGTGACCTTGCTTATTTAGCAGAAGCTGATTCTAGTGTTCACTCGGAATGGGACTCCTTTTGTAGCATTATAATGGAAATGTGTGGATCAAGTGCCACTTCTAAAAAGATTTCAAATCCAATGCCTCAGTCATCTTGGGAGTTTCTTATTCACAGTAAATTTCACAACAATTTCTGCAAACATAACTTTATTACTGAAAACTCTTCAGTAACATCTTTGAATGTGCGAAGAGTGGACTCCTCTCGGATAAATTCAGATGGTACAAAAAGGTCAGATAAAACATTTTATCCAAAGCTGTTGATGGAGAGTTTACATTGTTTGCATGCAGTATATGAGAGCCTGAAACTGAACAGTCTTCGCAAAAGGGACTTGGAGCTTCTGGGATTTCTATTATGCAATATTGCTAAGTTTCTGGGTAAGGAAAGTTATGTGGACCATTACATTCGTGATTTTCCTGGCCTTTCTGGAACTGTTGGAATATGTGAAACATCGTTTTCACAGGAAACTTCTCCAAGTTTATTCAGGTGGCTTGAAAACTGTTTGCTACATGGATATAATTCAGCTAGTATTAATGATCTTCCACCATTGATTCGTAAAGATGGGAATTCTGTTGTAAGCTGGGCCCGGAAGATAGTCTCATTTTATAGTTTGTTATCTGGTGCTAAACACATAGGGAAGCAACTGTCATCTGGTGTTTACTGTAATATTGCCACTGGATCATACAGCACAAATGAGGAACTCACAGTTCTGGCAATGGTTGGCGAAAAGTTTGGACTGCAACAGCTTGACTCATTGCCTTCTGGTGTGTCTCTTCCTTTGCGACATGCACTGGGTAAGTGCCGGGAATCTCCTCCCACTGGTTGGCCTGCAGCTGCATATGTTCTTCTTGGTCGGGAAGATTTGGCTTTGTCATGTCTTGAACGATCGTGCAAATCTAAGGAAATTGAAACCCAGACTAATGTGAACTTAATCTCTATGTCTGCACCTTACATGCTACATTTGCATCCAGTCACAATTCCTTCTGCAGTTTCTGATACAACTGGATTGGAGAATACTAAGTTTGAAGATGCAGATTCTGTTGATGGGTCTACGATTGATGGCATGGAGCATATCTTCAACACTAGCACACAGTTGCGTTATGGTCGTGATTTACGACTCAATGAGGTTAGACGTCTCCTCTGCTCAGCAAGACCTGTGGCAATTCAAACATCTGTTAATCCTAGTGCCTCTGATCAGGACCTCCAACAGGCCCAGCTATGGCACCTTGCACAAAGGACCACCGCCCTTCCTTTTGGTCGTGGGGCATTCACTCTTGCTACCATATATACACTTTTGACAGAGGCCTTTGCAGTCCCCAAGCTTGTTTTAGCAGGTCGGTTGCCTGCCCAGCAAAATGCAACTGTTAATCTGGACCCAAATGTAAAGAATATACAAGAAATCAAATCTTGGCCTGAATTCAACAATGCTGTTGCCGCTGGACTAAGGCTGGCTCCTCTTCAGGGAAAAATGTCAAGAATGTGGATAATATACAACAAACCAGAGGAGCCAAATGCTATTCATGCTGGACTTATCCTTGCGCTGGGATTACATGGTTATCTTCGTGTTTTAACTATTACGGACATATATCAGTATCTTTATCAGGAACATGAGACCACAACGGTTGGTATGATGCTCGGCCTGGCAGCTTCTTACAGAGGTACTATGCAACCAGCAATATCTAAGGTTCTTTATTTTCATATACCTGCTCGACTTCCAGTTGCAAGTGAAGTAGAGTTACCAACCCTTCTGCAGTCAGCAGGTCTAATGTCAGTTGGGCTTCTTTATGAAGGATCCGCACATCCACAAACAATGCAGATTCTCTTGTTGGAAATAGGACGTAGAAGTGCAGGGGATAATGTTCTTGAAAGGGAGGGTTATGCTGTTTCTGCAGGATTTTCATTGGGTCTTGTTGCATTGGGTCGGGGAGAAGATGCTCTTGGTTTCATGGACACAATGGTTGATAAATTGTTCCATTATATTGGTGGAAAAGAAGTTAACAATGATAGATCCCTTTCATCAATATTATCAGCCGATGAACACAGCCGTGCTGCTGCGCAGATGATGGATGGAACTGCAGTCAATGTTGATGCCACTGCACCTGGAGCCACACTCGCTCTTGCTTTAATGTTTTTGAAGACTGAATCACAGGTGATTGTGTCTAAGCTCTCTATTCCTCAAACACAGTTTGAGTTACAATATGTGAGGCCTGATTTCATCATGCTTCGTGTCATTGCCCGAAATTTGATAATGTGGAGCAGGATTCATCCTTCCCAAGATTGGGTAGAGTCTCAGATTCCTGATATTGTCAAAAATGGTGTCAAATGCCTTGGGGATGATGCTGATGATATTGATGAAATGGATGCTGAAGCATTTGTGCAGGCATATGTCAATATAGTGGCTGGAGCCTGCATTTCCCTTGGGTTGAGATTTGCTGGTACAAAGGATGCAAATGCTCAGGAGTTACTTTACAGCTATGCTGTTTACTTTCTGAACGAGATCGATCCAGTTTCTGCTACAAGTGGAACCTTCCCAAAGGGATTGTCTCACTATGTCGATCGAAGCACATTGGAGATATGTCTTCATCTCATTGTTCTTTCCCTGTCCGTGGTTATGGCTGGTTCTGGACATTTACAAACCTTTAAGCTGTTGAGATTTCTCCGCAACAGAAATTCTGCAGATGGACATGTTAATTATGGTGTTCAGATGGCTGTAAGCCTAGCAATTGGTTTCTTGTTCCTTGGGGGAGGAACACGGACATTTTCAACAAGCAACAGTTCAGTTGCCGCTTTGCTGATTACTCTCTATCCACGATTGCCTACTGGACCAAGTGATAATCGATGCCACCTCCAGGCATTTCGGCATTTGTATGTACTTGCAACTGAAGCCCGTTGGATCCAGACGGTTGATGTTGACACTGGTCTACCTGTGTATGCCCCTCTTGAAGTTACTATTAAGGAAACTGAACATTATTCGGAAACAAGTTTTTGTGAGGTTACGGCTTGCCTTCTACCAGAACGTGACATTTTGAAGTCAATACGCATTTGTGGCCCTCGGTATTGGCCACAAATAATAGATCTTGTCCCTGAAGATGAACCTTGGTGGACCCCTGGGGACAAGAATAGCCCATTTAATTCTGGTGTTGTGTATATCAAAAGAAAGGTTGGAGCATGTTCGTACATTGATGATCCCATAGGGTGTCAGTCACTGCTGTCACGGGCAATGCATAAGGTGTTTGGTTTGACAAGTTTAAAAGGACGTGATCCCTGGTCAAGCGGGGACTATGGACCTGGTTCTGTTACTGTTGACCAGTTGGTTGGTACGTTCTCATCTGATCCTAGCTTGCTTGCTTTTGCACAACTCTGCTGCGACCCTTCCTGGAAGAGCAAATCTGACATTAATTTCCAAGAATTTTGCTTGCAAGTATTATTCGAGTGTGTGAGCAAGGACAGACCTGCTCTTTTACAGGTCTATTTGTCATTATACACGACAATTGGATCAATGGCAGGTCAAGTTACCAGTGATGCTGTTGTTTTATCCGACTCTCTTGCTATTTGTAATCTTAAGCTTGCACTAGCCTACAACGAGACTTTGTTAAGCGGAAGATCCACTGCTTCGAGAGGCGGCATTGTTCAATCCAACTTTATAGCATGTCTTAGGAAGCAGGTGGAAGAGCTCCTAAACTGTTCTCAGGACTTGAAGGACGACTTTTGTAATTATATGTATTCTGGGAGGTGGCCAAATGGGGAATTCCACGGAGATAAACGCTCGATACTTCTCTCTTGGTACCTTCAGTGGTTCGGTGTACCAGCTCCATCTGTAATTAAGATGGCTGTGGAGAAAGTCAAGCCCAAGCTCAAGTCCTCGTCAACTTTGGTCCCTTTGTTACATCTACTATTTCCGAGGACTCACATCAATGCTATTGCCGCGATAGATAATCTTTTTTCATCTAACGAGAGCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009553 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0010154 GO:0010252 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0042592 GO:0048229 GO:0048316 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166

Pfam Domains

Protein Families

ANAPC1 PC_rep

Protein Analysis

1828

Amino Acids

201.62

Weight (kDa)

6.08

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANAPC1	PF12859	34 - 274	8.6e-33	Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
ANAPC1	PF12859	294 - 518	1.3e-31	Anaphase-promoting complex subunit 1 WD40 beta-propeller domain
Apc1_MidN	PF20518	536 - 796	3.4e-21	Anaphase-promoting complex subunit 1 middle domain
APC1_3rd	PF21282	1470 - 1533	3.2e-15	APC1 beta sandwich domain
APC1_C	PF18122	1610 - 1776	3.8e-32	Anaphase-promoting complex sub unit 1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007863)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05560 AT5G05560 AT5G05560
fragaria_vesca	FvH4_7g24630 FvH4_7g24630 FvH4_7g24630 FvH4_7g24630 FvH4_7g24630 FvH4_7g24630
malus_domestica	MD07G1223400.v1.1
prunus_persica	Prupe.2G252000_v2.0.a1 Prupe.2G252000_v2.0.a1
pyrus_communis	pycom01g17190 pycom07g20210
rosa_chinensis	RchiOBHm_Chr1g0370461
rosa_laevigata	RLG00000027039
rosa_multiflora	Rmu_sc0005656.1_g000015
rosa_roxburghii	Rroxscaffold_4G00286540
rosa_rugosa	Rorug01G0356900
rosa_samantha	Rh1AG365400 Rh1BG328400 Rh1CG341500 Rh1DG358700
rosa_wichuraiana	Rw1G032210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1553
AarI	CACCTGC	1 cut(s)	5173
AatII	GACGTC	1 cut(s)	2656
Acc16I	TGCGCA	1 cut(s)	3576
Acc36I	ACCTGC	7 cut(s)	1090, 2843, 3232, 3269, 4981, 5015, 5173
Acc65I	GGTACC	1 cut(s)	5304
AccB1I	GGYRCC	3 cut(s)	911, 2742, 5304
AccB7I	CCANNNNNTGG	2 cut(s)	826, 2343
AccI	GTMKAC	2 cut(s)	2570, 4441
AccII	CGCG	3 cut(s)	185, 253, 5447
AcoI	YGGCCR	4 cut(s)	12, 1311, 4580, 5255
AcuI	CTGAAG	9 cut(s)	434, 483, 1316, 1671, 2963, 3906, 4422, 4626, 5294
AcyI	GRCGYC	1 cut(s)	2653
AfeI	AGCGCT	1 cut(s)	330
AflII	CTTAAG	1 cut(s)	5081
AflIII	ACRYGT	1 cut(s)	4204
AgeI	ACCGGT	1 cut(s)	245
AjiI	CACGTC	1 cut(s)	4778
AjnI	CCWGG	9 cut(s)	1398, 1936, 3159, 3619, 4368, 4627, 4784, 4807, 4895
AjuI	GAANNNNNNNTTGG	2 cut(s)	3441, 3473
AleI	CACNNNNGTG	1 cut(s)	3564
Alw21I	GWGCWC	2 cut(s)	36, 5196
Alw26I	GTCTC	9 cut(s)	285, 394, 2122, 2295, 2660, 3125, 3813, 4068, 5099
AlwNI	CAGNNNCTG	8 cut(s)	857, 1100, 1294, 1508, 2513, 2558, 4031, 4726
Aor51HI	AGCGCT	1 cut(s)	330
ApaI	GGGCCC	1 cut(s)	2107
ArsI	GACNNNNNNTTYG	4 cut(s)	3822, 3854, 4046, 4078
AseI	ATTAAT	3 cut(s)	59, 2052, 4917
AsiGI	ACCGGT	1 cut(s)	245
Asp700I	GAANNNNTTC	1 cut(s)	330
Asp718I	GGTACC	1 cut(s)	5304
AspA2I	CCTAGG	1 cut(s)	1118
AspLEI	GCGC	4 cut(s)	187, 331, 3061, 3577
AsuC2I	CCSGG	3 cut(s)	620, 2107, 2324
AsuHPI	GGTGA	4 cut(s)	91, 392, 1496, 3682
AvaII	GGWCC	9 cut(s)	248, 1915, 2719, 2757, 2893, 4343, 4623, 4805, 5395
AvrII	CCTAGG	1 cut(s)	1118
BaeGI	GKGCMC	2 cut(s)	916, 2107
BalI	TGGCCA	2 cut(s)	4582, 5257
BamHI	GGATCC	2 cut(s)	3311, 4413
BanI	GGYRCC	3 cut(s)	911, 2742, 5304
BanII	GRGCYC	2 cut(s)	2107, 5196
BarI	GAAGNNNNNNTAC	6 cut(s)	101, 133, 414, 446, 3240, 3272
BauI	CACGAG	1 cut(s)	206
BbsI	GAAGAC	3 cut(s)	1829, 3662, 4091
Bbv12I	GWGCWC	2 cut(s)	36, 5196
BbvCI	CCTCAGC	1 cut(s)	1428
BceAI	ACGGC	5 cut(s)	34, 262, 309, 3549, 4533
BcgI	CGANNNNNNTGC	2 cut(s)	5048, 5082
BciT130I	CCWGG	9 cut(s)	1400, 1938, 3161, 3621, 4370, 4629, 4786, 4809, 4897
BclI	TGATCA	1 cut(s)	2713
BcnI	CCSGG	3 cut(s)	620, 2107, 2324
BcoDI	GTCTC	9 cut(s)	285, 394, 2122, 2295, 2660, 3125, 3813, 4068, 5099
BfmI	CTRYAG	8 cut(s)	1083, 1450, 2349, 2505, 3269, 3408, 3594, 4194
BfoI	RGCGCY	1 cut(s)	332
BfrI	CTTAAG	1 cut(s)	5081
BfuAI	ACCTGC	7 cut(s)	1090, 2843, 3232, 3269, 4981, 5015, 5173
BglII	AGATCT	2 cut(s)	523, 4594
BlnI	CCTAGG	1 cut(s)	1118
BlpI	GCTNAGC	2 cut(s)	188, 2665
Bme18I	GGWCC	9 cut(s)	248, 1915, 2719, 2757, 2893, 4343, 4623, 4805, 5395
BmgBI	CACGTC	1 cut(s)	4778
BmrI	ACTGGG	1 cut(s)	2321
BmuI	ACTGGG	1 cut(s)	2321
BoxI	GACNNNNGTC	1 cut(s)	4437
BpiI	GAAGAC	3 cut(s)	1829, 3662, 4091
BplI	GAGNNNNNCTC	2 cut(s)	3615, 3647
BpmI	CTGGAG	5 cut(s)	267, 1093, 3642, 3942, 4352
Bpu10I	CCTNAGC	2 cut(s)	1428, 3975
Bpu1102I	GCTNAGC	2 cut(s)	188, 2665
BpuEI	CTTGAG	1 cut(s)	5360
BpuMI	CCSGG	3 cut(s)	620, 2107, 2324
Bsa29I	ATCGAT	2 cut(s)	4020, 4357
BsaAI	YACGTR	1 cut(s)	1379
BsaHI	GRCGYC	1 cut(s)	2653
BsaI	GGTCTC	1 cut(s)	3125
BsaWI	WCCGGW	1 cut(s)	245
BsaXI	ACNNNNNCTCC	2 cut(s)	510, 540
Bse118I	RCCGGY	1 cut(s)	245
Bse3DI	GCAATG	6 cut(s)	272, 1201, 2241, 2273, 3750, 4746
BseBI	CCWGG	9 cut(s)	1400, 1938, 3161, 3621, 4370, 4629, 4786, 4809, 4897
BseCI	ATCGAT	2 cut(s)	4020, 4357
BseGI	GGATG	8 cut(s)	166, 2485, 3319, 3592, 3784, 3859, 3886, 3970
BseMI	GCAATG	6 cut(s)	272, 1201, 2241, 2273, 3750, 4746
BseMII	CTCAG	8 cut(s)	179, 1301, 1419, 1622, 2679, 3824, 3989, 5222
BseRI	GAGGAG	5 cut(s)	1711, 2322, 2648, 2964, 3038
BseSI	GKGCMC	2 cut(s)	916, 2107
BseYI	CCCAGC	4 cut(s)	2100, 2733, 2868, 3061
BsgI	GTGCAG	4 cut(s)	2448, 3387, 3600, 3917
Bsh1236I	CGCG	3 cut(s)	185, 253, 5447
Bsh1285I	CGRYCG	3 cut(s)	196, 2408, 4078
BshNI	GGYRCC	3 cut(s)	911, 2742, 5304
BshTI	ACCGGT	1 cut(s)	245
BshVI	ATCGAT	2 cut(s)	4020, 4357
BsiEI	CGRYCG	3 cut(s)	196, 2408, 4078
BsiHKAI	GWGCWC	2 cut(s)	36, 5196
BsiSI	CCGG	4 cut(s)	246, 620, 2107, 2323
BsiWI	CGTACG	1 cut(s)	517
BsmAI	GTCTC	9 cut(s)	285, 394, 2122, 2295, 2660, 3125, 3813, 4068, 5099
BsmBI	CGTCTC	2 cut(s)	285, 2660
BsmI	GAATGC	1 cut(s)	2786
Bso31I	GGTCTC	1 cut(s)	3125
Bsp120I	GGGCCC	1 cut(s)	2103
Bsp1286I	GDGCHC	4 cut(s)	36, 916, 2107, 5196
Bsp1720I	GCTNAGC	2 cut(s)	188, 2665
Bsp19I	CCATGG	1 cut(s)	1422
BspCNI	CTCAG	8 cut(s)	180, 1300, 1420, 1621, 2678, 3823, 3988, 5221
BspDI	ATCGAT	2 cut(s)	4020, 4357
BspFNI	CGCG	3 cut(s)	185, 253, 5447
BspMAI	CTGCAG	7 cut(s)	1087, 2353, 2509, 3273, 3412, 3598, 4198
BspMI	ACCTGC	7 cut(s)	1090, 2843, 3232, 3269, 4981, 5015, 5173
BspQI	GCTCTTC	2 cut(s)	4895, 5184
BspT107I	GGYRCC	3 cut(s)	911, 2742, 5304
BspTI	CTTAAG	1 cut(s)	5081
BspTNI	GGTCTC	1 cut(s)	3125
BsrDI	GCAATG	6 cut(s)	272, 1201, 2241, 2273, 3750, 4746
BsrFI	RCCGGY	1 cut(s)	245
BssAI	RCCGGY	1 cut(s)	245
BssNI	GRCGYC	1 cut(s)	2653
BssSI	CACGAG	1 cut(s)	206
BssT1I	CCWWGG	7 cut(s)	603, 1118, 1422, 3847, 3937, 4258, 4616
Bst2BI	CACGAG	1 cut(s)	206
Bst2UI	CCWGG	9 cut(s)	1400, 1938, 3161, 3621, 4370, 4629, 4786, 4809, 4897
Bst6I	CTCTTC	9 cut(s)	178, 435, 535, 1690, 1705, 2298, 2982, 4895, 5184
BstACI	GRCGYC	1 cut(s)	2653
BstAFI	CTTAAG	1 cut(s)	5081
BstAPI	GCANNNNNTGC	1 cut(s)	857
BstBAI	YACGTR	1 cut(s)	1379
BstDSI	CCRYGG	3 cut(s)	1422, 4125, 5272
BstEII	GGTNACC	1 cut(s)	1484
BstENI	CCTNNNNNAGG	1 cut(s)	2726
BstF5I	GGATG	8 cut(s)	166, 2485, 3319, 3592, 3784, 3859, 3886, 3970
BstFNI	CGCG	3 cut(s)	185, 253, 5447
BstH2I	RGCGCY	1 cut(s)	332
BstHHI	GCGC	4 cut(s)	187, 331, 3061, 3577
BstMAI	GTCTC	9 cut(s)	285, 394, 2122, 2295, 2660, 3125, 3813, 4068, 5099
BstMCI	CGRYCG	3 cut(s)	196, 2408, 4078
BstNI	CCWGG	9 cut(s)	1400, 1938, 3161, 3621, 4370, 4629, 4786, 4809, 4897
BstNSI	RCATGY	8 cut(s)	485, 1156, 1808, 2309, 2476, 4208, 4692, 5172
BstPAI	GACNNNNGTC	1 cut(s)	4437
BstPI	GGTNACC	1 cut(s)	1484
BstSFI	CTRYAG	8 cut(s)	1083, 1450, 2349, 2505, 3269, 3408, 3594, 4194
BstSLI	GKGCMC	2 cut(s)	916, 2107
BstSNI	TACGTA	1 cut(s)	1379
BstUI	CGCG	3 cut(s)	185, 253, 5447
BstV2I	GAAGAC	3 cut(s)	1829, 3662, 4091
BstX2I	RGATCY	7 cut(s)	523, 646, 3311, 3527, 4413, 4594, 5123
BstXI	CCANNNNNNTGG	2 cut(s)	3500, 4835
BstYI	RGATCY	7 cut(s)	523, 646, 3311, 3527, 4413, 4594, 5123
Bsu15I	ATCGAT	2 cut(s)	4020, 4357
BsuTUI	ATCGAT	2 cut(s)	4020, 4357
BtgI	CCRYGG	3 cut(s)	1422, 4125, 5272
BtrI	CACGTC	1 cut(s)	4778
BtsCI	GGATG	8 cut(s)	166, 2485, 3319, 3592, 3784, 3859, 3886, 3970
BtsI	GCAGTG	3 cut(s)	3612, 4724, 5127
BtsIMutI	CAGTG	9 cut(s)	2193, 2309, 2337, 3612, 4434, 4724, 5044, 5127, 5318
BveI	ACCTGC	7 cut(s)	1090, 2843, 3232, 3269, 4981, 5015, 5173
CaiI	CAGNNNCTG	8 cut(s)	857, 1100, 1294, 1508, 2513, 2558, 4031, 4726
CfoI	GCGC	4 cut(s)	187, 331, 3061, 3577
Cfr10I	RCCGGY	1 cut(s)	245
ClaI	ATCGAT	2 cut(s)	4020, 4357
CpoI	CGGWCCG	1 cut(s)	248
CsiI	ACCWGGT	1 cut(s)	4807
CspAI	ACCGGT	1 cut(s)	245
CspCI	CAANNNNNGTGG	4 cut(s)	2658, 2693, 3124, 3159
CspI	CGGWCCG	1 cut(s)	248
DraI	TTTAAA	1 cut(s)	4770
EaeI	YGGCCR	4 cut(s)	12, 1311, 4580, 5255
Eam1104I	CTCTTC	9 cut(s)	178, 435, 535, 1690, 1705, 2298, 2982, 4895, 5184
EarI	CTCTTC	9 cut(s)	178, 435, 535, 1690, 1705, 2298, 2982, 4895, 5184
Ecl136II	GAGCTC	1 cut(s)	5194
Eco105I	TACGTA	1 cut(s)	1379
Eco130I	CCWWGG	7 cut(s)	603, 1118, 1422, 3847, 3937, 4258, 4616
Eco147I	AGGCCT	2 cut(s)	2825, 3727
Eco24I	GRGCYC	2 cut(s)	2107, 5196
Eco31I	GGTCTC	1 cut(s)	3125
Eco47I	GGWCC	9 cut(s)	248, 1915, 2719, 2757, 2893, 4343, 4623, 4805, 5395
Eco47III	AGCGCT	1 cut(s)	330
Eco53kI	GAGCTC	1 cut(s)	5194
Eco57I	CTGAAG	9 cut(s)	434, 483, 1316, 1671, 2963, 3906, 4422, 4626, 5294
Eco91I	GGTNACC	1 cut(s)	1484
EcoICRI	GAGCTC	1 cut(s)	5194
EcoNI	CCTNNNNNAGG	1 cut(s)	2726
EcoO109I	RGGNCCY	2 cut(s)	1291, 2719
EcoO65I	GGTNACC	1 cut(s)	1484
EcoRI	GAATTC	4 cut(s)	744, 2086, 2936, 5267
EcoRII	CCWGG	9 cut(s)	1398, 1936, 3159, 3619, 4368, 4627, 4784, 4807, 4895
EcoT14I	CCWWGG	7 cut(s)	603, 1118, 1422, 3847, 3937, 4258, 4616
EcoT22I	ATGCAT	1 cut(s)	4746
EcoT38I	GRGCYC	2 cut(s)	2107, 5196
ErhI	CCWWGG	7 cut(s)	603, 1118, 1422, 3847, 3937, 4258, 4616
Esp3I	CGTCTC	2 cut(s)	285, 2660
FalI	AAGNNNNNCTT	6 cut(s)	1564, 1596, 3441, 3473, 5210, 5242
FauI	CCCGC	3 cut(s)	94, 1121, 4787
FauNDI	CATATG	2 cut(s)	2359, 3904
FbaI	TGATCA	1 cut(s)	2713
FblI	GTMKAC	2 cut(s)	2570, 4441
FokI	GGATG	8 cut(s)	173, 2472, 3306, 3599, 3771, 3866, 3893, 3977
FriOI	GRGCYC	2 cut(s)	2107, 5196
FspI	TGCGCA	1 cut(s)	3576
GlaI	GCGC	4 cut(s)	186, 330, 3060, 3576
GsaI	CCCAGC	4 cut(s)	2104, 2737, 2872, 3065
GsuI	CTGGAG	5 cut(s)	267, 1093, 3642, 3942, 4352
HaeII	RGCGCY	1 cut(s)	332
HapII	CCGG	4 cut(s)	246, 620, 2107, 2323
HhaI	GCGC	4 cut(s)	187, 331, 3061, 3577
Hin1I	GRCGYC	1 cut(s)	2653
Hin6I	GCGC	4 cut(s)	185, 329, 3059, 3575
HinP1I	GCGC	4 cut(s)	185, 329, 3059, 3575
HincII	GTYRAC	5 cut(s)	243, 3517, 4432, 4825, 5388
HindII	GTYRAC	5 cut(s)	243, 3517, 4432, 4825, 5388
HindIII	AAGCTT	2 cut(s)	2840, 5084
HpaI	GTTAAC	1 cut(s)	3517
HpaII	CCGG	4 cut(s)	246, 620, 2107, 2323
HphI	GGTGA	4 cut(s)	91, 392, 1496, 3682
Hpy99I	CGWCG	2 cut(s)	80, 225
HpyCH4IV	ACGT	7 cut(s)	96, 1378, 2653, 3358, 4537, 4777, 4841
HpySE526I	ACGT	7 cut(s)	96, 1378, 2653, 3358, 4537, 4777, 4841
Hsp92I	GRCGYC	1 cut(s)	2653
HspAI	GCGC	4 cut(s)	185, 329, 3059, 3575
KpnI	GGTACC	1 cut(s)	5308
Ksp22I	TGATCA	1 cut(s)	2713
KspAI	GTTAAC	1 cut(s)	3517
LguI	GCTCTTC	2 cut(s)	4895, 5184
MabI	ACCWGGT	1 cut(s)	4807
MaeII	ACGT	7 cut(s)	96, 1378, 2653, 3358, 4537, 4777, 4841
MfeI	CAATTG	2 cut(s)	4242, 5010
MflI	RGATCY	7 cut(s)	523, 646, 3311, 3527, 4413, 4594, 5123
MhlI	GDGCHC	4 cut(s)	36, 916, 2107, 5196
MlsI	TGGCCA	2 cut(s)	4582, 5257
MluNI	TGGCCA	2 cut(s)	4582, 5257
MlyI	GAGTC	8 cut(s)	164, 1529, 1712, 2264, 2631, 3815, 5054, 5419
MmeI	TCCRAC	7 cut(s)	1936, 2749, 3327, 4391, 4663, 5082, 5181
Mox20I	TGGCCA	2 cut(s)	4582, 5257
Mph1103I	ATGCAT	1 cut(s)	4746
MroXI	GAANNNNTTC	1 cut(s)	330
MscI	TGGCCA	2 cut(s)	4582, 5257
MslI	CAYNNNNRTG	6 cut(s)	421, 1421, 1553, 3564, 4386, 5433
Msp20I	TGGCCA	2 cut(s)	4582, 5257
MspA1I	CMGCKG	3 cut(s)	461, 2354, 2957
MspCI	CTTAAG	1 cut(s)	5081
MspI	CCGG	4 cut(s)	246, 620, 2107, 2323
MunI	CAATTG	2 cut(s)	4242, 5010
Mva1269I	GAATGC	1 cut(s)	2786
MvaI	CCWGG	9 cut(s)	1400, 1938, 3161, 3621, 4370, 4629, 4786, 4809, 4897
MvnI	CGCG	3 cut(s)	185, 253, 5447
NciI	CCSGG	3 cut(s)	620, 2107, 2324
NcoI	CCATGG	1 cut(s)	1422
NdeI	CATATG	2 cut(s)	2359, 3904
NmeAIII	GCCGAG	2 cut(s)	15, 3135
NmuCI	GTSAC	8 cut(s)	97, 412, 580, 1484, 2491, 4538, 4722, 4731
NsbI	TGCGCA	1 cut(s)	3576
NsiI	ATGCAT	1 cut(s)	4746
NspI	RCATGY	8 cut(s)	485, 1156, 1808, 2309, 2476, 4208, 4692, 5172
OliI	CACNNNNGTG	1 cut(s)	3564
PaeI	GCATGC	1 cut(s)	1808
PaqCI	CACCTGC	1 cut(s)	5173
PasI	CCCWGGG	1 cut(s)	4628
PceI	AGGCCT	2 cut(s)	2825, 3727
PciI	ACATGT	1 cut(s)	4204
PciSI	GCTCTTC	2 cut(s)	4895, 5184
PcsI	WCGNNNNNNNCGW	1 cut(s)	2632
PctI	GAATGC	1 cut(s)	2786
PdmI	GAANNNNTTC	1 cut(s)	330
PfeI	GAWTC	9 cut(s)	267, 1511, 2071, 2327, 2555, 3337, 3662, 3781, 3814
Pfl23II	CGTACG	1 cut(s)	517
PflFI	GACNNNGTC	1 cut(s)	555
PflMI	CCANNNNNTGG	2 cut(s)	826, 2343
PfoI	TCCNGGA	1 cut(s)	4895
PinAI	ACCGGT	1 cut(s)	245
Ple19I	CGATCG	3 cut(s)	196, 2408, 4078
PleI	GAGTC	8 cut(s)	164, 1529, 1712, 2264, 2631, 3814, 5054, 5419
PpsI	GAGTC	8 cut(s)	164, 1529, 1712, 2264, 2631, 3814, 5054, 5419
Ppu21I	YACGTR	1 cut(s)	1379
PpuMI	RGGWCCY	1 cut(s)	2719
PscI	ACATGT	1 cut(s)	4204
PshAI	GACNNNNGTC	1 cut(s)	4437
PshBI	ATTAAT	3 cut(s)	59, 2052, 4917
PsiI	TTATAA	1 cut(s)	1553
Psp124BI	GAGCTC	1 cut(s)	5196
Psp5II	RGGWCCY	1 cut(s)	2719
Psp6I	CCWGG	9 cut(s)	1398, 1936, 3159, 3619, 4368, 4627, 4784, 4807, 4895
PspEI	GGTNACC	1 cut(s)	1484
PspFI	CCCAGC	4 cut(s)	2100, 2733, 2868, 3061
PspGI	CCWGG	9 cut(s)	1398, 1936, 3159, 3619, 4368, 4627, 4784, 4807, 4895
PspLI	CGTACG	1 cut(s)	517
PspOMI	GGGCCC	1 cut(s)	2103
PspPPI	RGGWCCY	1 cut(s)	2719
PsrI	GAACNNNNNNTAC	2 cut(s)	837, 869
PstI	CTGCAG	7 cut(s)	1087, 2353, 2509, 3273, 3412, 3598, 4198
PstNI	CAGNNNCTG	8 cut(s)	857, 1100, 1294, 1508, 2513, 2558, 4031, 4726
PsuI	RGATCY	7 cut(s)	523, 646, 3311, 3527, 4413, 4594, 5123
PsyI	GACNNNGTC	1 cut(s)	555
PvuI	CGATCG	3 cut(s)	196, 2408, 4078
PvuII	CAGCTG	2 cut(s)	461, 2354
RseI	CAYNNNNRTG	6 cut(s)	421, 1421, 1553, 3564, 4386, 5433
Rsr2I	CGGWCCG	1 cut(s)	248
RsrII	CGGWCCG	1 cut(s)	248
SacI	GAGCTC	1 cut(s)	5196
SapI	GCTCTTC	2 cut(s)	4895, 5184
SchI	GAGTC	8 cut(s)	164, 1529, 1712, 2264, 2631, 3815, 5054, 5419
SduI	GDGCHC	4 cut(s)	36, 916, 2107, 5196
SexAI	ACCWGGT	1 cut(s)	4807
SfcI	CTRYAG	8 cut(s)	1083, 1450, 2349, 2505, 3269, 3408, 3594, 4194
SinI	GGWCC	9 cut(s)	248, 1915, 2719, 2757, 2893, 4343, 4623, 4805, 5395
SmiMI	CAYNNNNRTG	6 cut(s)	421, 1421, 1553, 3564, 4386, 5433
SmlI	CTYRAG	2 cut(s)	5081, 5375
SmoI	CTYRAG	2 cut(s)	5081, 5375
SnaBI	TACGTA	1 cut(s)	1379
SphI	GCATGC	1 cut(s)	1808
SseBI	AGGCCT	2 cut(s)	2825, 3727
SspI	AATATT	3 cut(s)	1882, 2188, 3543
SstI	GAGCTC	1 cut(s)	5196
StuI	AGGCCT	2 cut(s)	2825, 3727
StyI	CCWWGG	7 cut(s)	603, 1118, 1422, 3847, 3937, 4258, 4616
TaiI	ACGT	7 cut(s)	99, 1381, 2656, 3361, 4540, 4780, 4844
TaqII	GACCGA	1 cut(s)	338
TatI	WGTACW	1 cut(s)	4390
TauI	GCSGC	5 cut(s)	24, 2957, 4307, 5145, 5447
TfiI	GAWTC	9 cut(s)	267, 1511, 2071, 2327, 2555, 3337, 3662, 3781, 3814
TscAI	CASTG	9 cut(s)	2200, 2316, 2344, 3619, 4441, 4731, 5044, 5134, 5318
TseFI	GTSAC	8 cut(s)	97, 412, 580, 1484, 2491, 4538, 4722, 4731
Tsp45I	GTSAC	8 cut(s)	97, 412, 580, 1484, 2491, 4538, 4722, 4731
TspGWI	ACGGA	5 cut(s)	173, 3113, 4114, 4288, 5289
TspRI	CASTG	9 cut(s)	2200, 2316, 2344, 3619, 4441, 4731, 5044, 5134, 5318
Tth111I	GACNNNGTC	1 cut(s)	555
Van91I	CCANNNNNTGG	2 cut(s)	826, 2343
Vha464I	CTTAAG	1 cut(s)	5081
VpaK11BI	GGWCC	9 cut(s)	248, 1915, 2719, 2757, 2893, 4343, 4623, 4805, 5395
VspI	ATTAAT	3 cut(s)	59, 2052, 4917
XagI	CCTNNNNNAGG	1 cut(s)	2726
XceI	RCATGY	8 cut(s)	485, 1156, 1808, 2309, 2476, 4208, 4692, 5172
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3500
XmaJI	CCTAGG	1 cut(s)	1118
XmiI	GTMKAC	2 cut(s)	2570, 4441
XmnI	GAANNNNTTC	1 cut(s)	330
ZraI	GACGTC	1 cut(s)	2654
Zsp2I	ATGCAT	1 cut(s)	4746

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.